共查询到20条相似文献,搜索用时 93 毫秒
1.
目的 探索建立超快速液相色谱(UFLC)法测定复方丹参片中三七皂苷R1、人参皂苷Rg1及Rb1。方法 采用SHIMADZU Shim-pack XR-ODS Ⅲ(2.0 mm×75 mm, 1.6 μm)色谱柱;以乙腈-水作为流动相进行梯度洗脱;体积流量为0.4 mL/min;检测波长为203 nm;进样体积为3 μL。结果 三七皂苷R1、人参皂苷Rg1及Rb1分别在0.025 7~0.257 0、0.101 2~1.012 0、0.104 4~1.044 0 μg与峰面积呈良好的线性关系,平均加样回收率分别为96.7%、98.1%、98.8%。结论 本方法在15min内可以将三七皂苷R1、人参皂苷Rg1及Rb1有效分离,节省了大量人力和流动相的消耗,为中药的质量控制技术提供参考方法。 相似文献
2.
目的 建立HPLC-MS/MS同时测定同济2号颗粒中5个主要活性成分黄芪甲苷、绿原酸、人参皂苷Rg1、人参皂苷Rb1及三七皂苷R1的方法。方法 以水(0.1%甲酸)-乙腈(0.1%甲酸)为流动相,梯度洗脱,体积流量为0.4 mL/min。YMC-Pack Pro C8色谱柱分离,采用电喷雾离子源(ESI源),负离子模式,以选择性离子监测模式(SIM)进行测定。监测离子分别是m/z 829(黄芪甲苷)、m/z 353(绿原酸)、m/z 845(人参皂苷Rg1)、m/z 1 108(人参皂苷Rb1)、m/z 932(三七皂苷R1)。结果 黄芪甲苷、绿原酸、人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1分别在0.075~2.4、0.95~30.3、1.71~54.72、1.12~35.92、0.45~14.28 μg/mL与峰面积呈良好线性关系。结论 该方法专属性好,灵敏度高,准确快捷,适用于同济2号颗粒的快速检测,为该药的质量标准提供依据。 相似文献
3.
摘 要 目的:建立HPLC梯度法同时测定复方三七维康胶囊中三七皂苷R1、人参皂苷Rb1、Rg13种皂苷的含量。方法: 色谱柱:大连Spherisorb C18分析柱(200 mm×4.6 mm,5 μm);流动相:乙腈-水,梯度洗脱;流速:1.0 ml·min-1;检测波长 203 nm。结果:三七皂苷R1、人参皂苷Rg1和Rb1分别在1.06~18.55 μg(r=0.997 3)、2.02~35.35 μg(r=0.998 2)和2.02~35.35 μg(r=0.998 2)之间线性关系良好。平均回收率三七皂苷R1为100.8%(RSD=1.53%,n=5),人参皂苷 Rg1为98.9%(RSD=1.87%,n=5),人参皂苷Rb1为99.7%(RSD=1.90%,n=5)。结论:HPLC梯度洗脱法能够将多种皂苷很好地分离检测,该方法准确可靠,重复性好,可用于控制其质量。 相似文献
4.
目的 建立同时测定三七提取液中三七皂苷R2(S)、七叶胆苷XVII及人参皂苷F2含量的高效液相色谱法。方法 色谱柱为Waters Acquity UPLC CSH-C18(50 mm×2.1 mm,1.7 μm),流动相为0.01%甲酸-水(A)和0.01%甲酸-乙腈(B),梯度洗脱,检测波长为203 nm,进样量为5 μL,流速为0.35 mL·min-1,柱温为40 ℃。结果 在43 min内可完成三七皂苷R2(S)、七叶胆苷XVII和人参皂苷F2的分离测定。3种皂苷峰面积和浓度线性关系良好(r2>0.999 8);日内和日间精密度RSD<3.4%;回收率为98.4%~102.1%。结论 该方法简便、准确,重复性好,可用于三七提取液中皂苷成分的测定。 相似文献
5.
摘 要 目的: 建立同时测定人参三七颗粒中人参皂苷Rg1、Re、Rb1和三七皂苷R1含量的方法。方法: 采用HPLC法,色谱柱为Sunfire C18(150 mm×4.6 mm,5 μm)分析柱,流动相以乙腈-水梯度洗脱;检测波长为203 nm;柱温30℃;流速1.0 ml·min-1。结果:人参皂苷Rg1,Re,Rb1和三七皂苷R1之间有较好的分离度,4种成分在线性范围内与峰面积之间线性关系良好,人参皂苷Rg1、Re、Rb1和三七皂苷R1加样回收率分别为99.83%,97.84%,98.43%,97.34%,RSD分别为2.08%,1.66%,1.73%和1.42%(n=5)。结论:本方法可同时测定人参三七颗粒中的人参皂苷Rg1,Re,Rb1和三七皂苷R1含量。 相似文献
6.
羽叶三七叶中甙类成分的研究 总被引:3,自引:0,他引:3
从羽叶三七叶中分离到十三种甙类成分,经FAB-MS,13CNMR谱,双照射1HN-MR谱,1H-1H COSY谱及与标准品直接对照,证明十一种为已知化合物,分别为人参皂甙F1(Ⅰ),F2(Ⅱ),F3(Ⅲ),Rg2(Ⅳ),Ra(Ⅴ),Rd(Ⅵ),Rb1(Ⅷ),Rb3(Ⅷ),24(S)-假人参甙F11(Ⅸ),人参黄酮(Ⅹ)和珠子参甙F1(Ⅺ);另外两种为新的达玛烷型皂甙,命名为羽叶三七甙F1(Ⅻ)和F2(ⅫⅠ),并确定其化学结构。同时修正珠子参甙F3的结构。进一步阐明人参黄酮甙结构中的两个糖的连接方式。 相似文献
7.
目的 对活骨丸进行质量标准研究。方法 采用薄层色谱法,对制剂中丹参、地黄、当归、川芎、三七、土鳖虫、制草乌进行专属性鉴别;采用高效液相色谱(HPLC)法对处方三七有效成分人参皂苷Rg1、Rb1和三七皂苷R1进行含量测定。以乙腈和水按不同比例混合后进行梯度洗脱,流速每为1.0 ml/min;柱温30℃;检测波长为203 nm;进样量为10 μl。结果 丹参、地黄、当归、川芎、三七、土鳖虫、制草乌等的薄层图谱特征斑点清晰,分离度好,阴性对照无干扰。三七皂苷R1、人参皂苷Rb1和Rg1分别在39.92~399.2、84.28~842.8 μg/ml和135.86~1 358.6 μg/ml范围内有良好的线性关系;三七皂苷R1、人参皂苷Rb1和人参皂苷Rg1的平均回收率分别为102.35%、103.84%、102.97%,RSD均小于2.0%。结论 所建立的质量标准准确、重复性良好,可用于活骨丸的质量控制。 相似文献
8.
9.
目的 建立同时测定跌打丸中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1含量的HPLC-ELSD检测方法。方法 采用Phenomenex Kinetex C18色谱柱(100 mm×4.6 mm,2.6 μm),柱温30 ℃,流速0.5 mL·min-1,流动相为乙腈-水,梯度洗脱,ELSD检测器,漂移管温度110 ℃,载气(空气)体积流量3.0 L·min-1。结果 三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1分别在0.158~3.16 μg(r=0.999 8),0.407~8.14 μg(r=0.999 5),0.446~8.92 μg(r=0.999 9)内呈良好的线性关系,平均加样回收率分别为97.55%(RSD=1.04%),98.09%(RSD=1.03%),97.34%(RSD=0.81%)。结论 该方法简便、快速、准确,可用于跌打丸的质量控制。 相似文献
10.
11.
12.
A total of 37 commercial samples of paeoniae radix (Paeonia lactiflora Pall. = P. albiflora Pall, and P. vitchii Lynch), were collected from Taiwan's herbal market. The contents of eight constituents (gallic acid, oxypaeoniflorin, albiflorin, paeoniflorin, benzoic acid, pentagalloylglucose, paeonol, and benzyoylalbiflorin) in these samples were determined by high-performance liquid chromatography. It was found that constituent contents in P. lactiflora samples were generally higher than in P. vitchii samples except for the albiflorin/oxypaeoniflorin ratio and paeonol content. The major compounds of P. lactiflora were paeoniflorin, albiflorin, and pentagalloylglucose, while those in P. vitchii were paeoniflorin, pentagalloylglucose, and oxypaeoniflorin. The peak-area ratio of albiflorin/oxypaeoniflorin was higher than unity in P. lactiflora samples but lower than unity in P. vitchii, and the ratio can be used to differentiate the origin of Paeonia species clearly. In addition, the former had higher contents in the core wood but the latter in the cortex. 相似文献
13.
14.
Insights into the neutral loss of 30 Da of Paeonia monoterpene glycosides in electrospray ionization tandem mass spectrometry and the rapid screening of monoterpene glycosides by LC/MS/MS 下载免费PDF全文
Monoterpene glycosides are the major bioactive compounds of Paeonia lactiflora Pall (P. lactiflora). Characteristic neutral loss of 30 Da has been extensively reported for monoterpene glycosides in tandem mass spectrometry. However, little is known about mechanism of this fragmentation. The neutral loss of 30 Da was studied for eleven monoterpene glycosides (1–11) from P. lactiflora by ion trap mass spectrometry in this report. Compounds 1–5 with a hemiacetal structure could readily lose 30 Da at low collision energy of 30% in MS/MS by ion trap mass spectrometry. For compounds 6–11, neutral loss of 30 Da could also be observed at low abundance, but the collision energy had to be increased to 60%. In both cases, high-accuracy mass spectrometry assigned the 30 Da as CH2O. After careful analysis of the structures and mass spectra, we believe that the neutral loss of 30 Da in compounds 1–5 was due to cleavage of the hemiacetal structure, whereas it was ascribed to the cleavage of 5¢-hydroxymethyl group of the glucosyl residue in other monoterpene glycosides. Furthermore, the characteristic neutral loss of 30 Da at low collision energy was used to screen hemiacetals from crude extracts of P. lactiflora and related plant species. Significant differences among Paeonia species were observed by 30 Da neutral loss analysis. 相似文献
15.
Yen PH Kiem PV Nhiem NX Tung NH Quang TH Minh CV Kim JW Choi EM Kim YH 《Archives of pharmacal research》2007,30(10):1179-1185
A new monoterpene glycoside, 6'-O-beta-D-glucopyranosylalbiflorin (1), and four known compounds; albiflorin (2), 6'-O-benzoylalbiflorin (3), paeoniflorin (4) and benzoyl paeoniflorin (5), were isolated from the methanolic extract of the roots of Paeonia lactiflora Pall.. Their chemical structures were completely elucidated using a combination of 2D NMR techniques (COSY, HMQC and HMBC) and HRESI-MS analyses. To investigate the bioactivities of these compounds, their effects on the differentiation of osteoblastic MC3T3-E1 cells were tested. Compound 1 (0.01-10 microM) significantly increased the alkaline phosphatase activity and nodules mineralization of MC3T3-E1 cells compared to those of the control (P<0.05). These results suggest that newly isolated compound 1 has a direct stimulatory effect on bone formation in vitro and may contribute to the prevention for osteoporosis. 相似文献
16.
17.
18.
Qiang Fu Shu-Bin Wang Shao-Hua Zhao Xiao-Juan Chen 《Journal of Asian natural products research》2013,15(7):697-702
Three new monoterpene glycosides, 2′-O-benzoylpaeoniflorin, albiflorin R2, and albiflorin R3 (1–3) were isolated from the roots of Paeonia lactiflora. Their structures were elucidated on the basis of spectroscopic means including one- and two-dimensional NMR experiments. 相似文献
19.
目的 揭示白芍不同发育时期不同组织部位中单萜苷含量及其生物合成基因的表达差异和两者之间的相关性。方法 通过HPLC测定白芍叶、花、果、根及根中不同部位(韧皮部、木质部、须根和根皮)在不同发育时期氧化芍药苷、白芍内酯苷、芍药苷和苯甲酰芍药苷含量。采用RT-PCR对筛选出来的8个白芍单萜苷生物合成相关基因进行相对含量测定。应用R语言分析4种单萜苷含量与各生物合成相关基因表达量的相关性。结果 芍药苷和苯甲酰芍药苷主要分布于根中,而氧化芍药苷和白芍内酯苷主要分布于花中。氧化芍药苷、芍药苷和苯甲酰芍药苷主要分布于木质部中,白芍内酯苷主要在韧皮部和须根。4种单萜苷成分在根中花期和果期含量均较低,在黄枯期中最高,展叶期次之。单萜苷含量与其关键酶基因表达量相关性分析结果表明,HMGR2和IDI基因与芍药苷、苯甲酰芍药苷和氧化芍药苷含量呈高度正相关。结论 白芍单萜苷及其生物合成相关基因存在时空特异表达模式,甲羟戉酸途径是白芍中生物合成萜类骨架的主要途径。 相似文献