本文通过石油烃分散液对华贵栉孔扇贝的急性毒性试验,经SPSS的probit分析结果表明,石油烃分散液对华贵栉孔扇贝的24 h LC50、48 h LC50、72 h LC50和96 h LC50浓度依次是233.08、147.30、81.67和75.16 mg/L,安全浓度Sc是17.65 mg/L。通过生物富集试验结果显示,华贵栉孔扇贝在120 h内对石油烃的富集效果与浓度和时间成正比。石油烃分散液胁迫下对华贵栉孔扇贝血清中POD、 SOD酶活的影响呈现出低浓度促进、高浓度抑制的特征,并对其肝、鳃组织AKP酶活性具有促进作用。 相似文献
软体动物碳酸钙贝壳的形成过程涉及到C a2+的吸收、转运、贮藏、分泌和沉积等,与钙代谢这一高度受控的复杂生理过程紧密相关。本研究以栉孔扇贝转录组测序结果为基础,通过RACE技术,从栉孔扇贝外套膜中克隆得到钙调素类似蛋白的cDNA序列,全长863 bp ,编码149个氨基酸,相应蛋白质的预测分子量约为17.0 ku ,理论等电点为4.03。钙调素类似蛋白具有4个可能的EF‐hand Ca2+结合结构域,属于 EF‐hand钙结合蛋白家族,其氨基酸序列与栉孔扇贝钙调素钙调素的相似度为66%。半定量RT‐PCR检测显示,钙调素类似蛋白基因在外套膜中特异性高表达,预示其可能参与贝壳的矿化过程。实时定量PCR检测显示,钙调素类似蛋白基因的表达水平随环境中Ca2+浓度的升高呈先升后降趋势,表明钙调素类似蛋白与外套膜中的钙代谢过程密切相关;在贝壳缺刻损伤后的再生过程中,钙调素类似蛋白基因表达量显著上升,暗示其积极参与到了贝壳的再生过程中。上述结果为进一步阐明钙调素类似蛋白基因的功能及其在栉孔扇贝生物矿化过程中的作用提供可能的理论依据。 相似文献
Gonad development and sexual differentiation are important biological processes. More insight is needed in these processes to improve selection and breeding efficiency in the production of culture animals for monosex farm operations of commercially important shellfish species. Foxl2 is the earliest known sex dimorphic marker of ovarian differentiation in mammals, but studies on foxl2 in invertebrate species are rare. This study revealed the gonadogenesis and expression characteristics of foxl2 during the gonadal differentiation in the scallop Chlamys farreri, an important commercial mollusk in China. The result showed that C. farreri gonad becomes visible once juveniles reach a shell height of 5.5 mm. Sex can be distinguished based on gonad histology in animals with a minimum shell height of 9 mm. Expression of Cf‐foxl2 (C. farreri foxl2) was first observed in cytoplasm of ovarian germ and follicle cells, just after the initiation of ovarian differentiation. Results indicate that Cf‐foxl2 can be used as an early sex‐marker gene, and may have participated in the regulation of ovary differentiation in C. farreri. 相似文献