针刺外关穴和假穴小脑激活的PET脑功能成像研究

目的观察针刺右侧外关穴后小脑局部葡萄糖代谢变化,研究针刺外关穴小脑的激活区域,探讨针刺穴位小脑的作用机制。方法将18例健康志愿者分为外关穴针刺组、外关穴假针刺组和假穴针刺组3组,每组6例,分别接受右侧外关穴针刺、右侧外关穴假针刺及外关穴旁开假穴点针刺干预,行正电子发射体层显像脑功能成像检查,所得图像采用SPM8软件包进行处理和分析。结果外关穴针刺组及假针刺组各1例因明显刺痛感或头部移动明显,其数据不作进一步统计。与外关穴假针刺组比较,针刺右侧外关穴激活的小脑脑区为左侧小脑前叶山顶及右侧小脑后叶下半月小叶。与假穴针刺组比较,针刺右侧外关穴激活的小脑脑区为右侧小脑后叶山坡、左侧小脑扁桃体、左侧小脑后叶下半月小叶、双侧小脑前叶山顶及双侧小脑后叶。结论针刺穴位特异激活了小脑部分区域。针刺外关穴的治疗作用可能是激活小脑前叶及后叶的结果。     

强化基本技能训练 培养创新实践型人才

目的：探讨针灸推拿学专业基本技能教学改革模式,激发学生实践创新能力。方法：在集体备课基础上,从硬件和软件两方面,即从改善实验教学环境、改革实验课程设计（包括教学内容、考核体系、教学方式等）着手,对针灸推拿专业基础课《经络腧穴学》《刺法灸法学》《推拿学》《功法手法学》《实验针灸学》进行实验教学改革。结果：加强了针灸推拿学技能教学实验中心的建设,规范了针灸推拿专业基本技能训练教学方法、教学内容、操作技术以及考核标准,并编写和修改实验报告,组织编著出版《针灸推拿实验教材》。形成了实验与理论教学穿插交替进行,老师指导与学生自主练习相结合的技能训练模式。结论：基本技能教学改革对于激发学生创新能力,培养高素质实践型中医药针灸推拿专业人才有着重要意义。     

针刺太冲穴治疗高血压机制研究

近年来有许多国内外学者将针刺太冲穴作为治疗高血压的有效手段,并进行了大量的研究探索。该文总结近年来的相关文献,通过针刺太冲穴引起内皮素、血清NO含量、肾素-血管紧张素-醛固酮系统、胰岛素抵抗等的改变,论述其对内分泌系统的调节;通过交感神经兴奋性的改变和神经肽的作用,论述针刺太冲穴对神经系统的调节;并介绍了针刺太冲穴对细胞信号转导途径的影响,从而起到降压作用。在此基础上,对针刺太冲穴治疗高血压的机制研究,进行小结和展望。     

Effect of Acupuncture on Proliferation and Differentiation of Neural Stem Cells in Brain Tissues of Rats with Traumatic Brain Injury

Objective:To observe the effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injuny.Methods:Thirty SD rats were randomly and equally allocated to the sham-operated,the model and the acupuncture groups.The traumatic brain injury model was established by the free drop method.For the rats in the acupuncture group,acupuncture was applied once a day for 7 days.Brain histotomy was carried out when treatments were completed.Immunohistochemical techniques were adopted to detect the cells that express nestin,neurofilament proteins(NF)-200 and glial fibrillary acidic proteins(GFAP),the markers of neural stem cells,neurons,astrocytes respectively.Results:Compared to the sham-operated group,the number of nestin-positive cells and NF-200-positive cells in brain tissues was decreased significantly in the model group(P<0.01),whereas the number of GFAP-positive cells was significantly increased (P<0.01).Compared to the model group,the positive cells of nestin,NF-200,GFAP in brain tissues in the acupuncture group were increased obviously(P<0.01).Conclusions:Acupuncture can significantly increase the number of nestin-positive cells,NF-200-positive cells and GFAP-positive cells,indicating the significant increase of neural stem cells,neurons and astrocytes in number.Acupuncture can improve neuranagenesis by promoting the proliferation and differentiation of neural stem cells in brain tissues.This might be one of the mechanisms for acupuncture to treat traumatic brain injury and to promote the repair of nervous function.     

对穴位-脑功能影像学研究现状的思考——基于功能连接与穴脑相关学说的探讨

目前穴位效应的脑功能影像学研究基本肯定了针刺穴位后脑内相关区域的影像学信号会产生不同程度的变化,然而这些变化中是否存在着某些潜在的规律性联系却是一个值得探讨的问题。利用脑功能影像学研究中"功能连接"的概念分析针刺作用下的穴-脑相关的作用机制,从目前血氧水平依赖的神经活动影像信号分析,过渡到基于神经生物学手段的基因表达分析,对于进一步探讨穴位的脑功能特异性有着较为积极的意义。     

穴位贴敷经皮给药药贴对哮喘豚鼠支气管肺组织中基质金属蛋白酶-9的影响

目的观察穴位经皮给药药贴对实验性哮喘豚鼠基质金属蛋白酶-9(MMP-9)的影响。方法将48只豚鼠随机分为正常对照组、模型组、地塞米松组和经皮给药药贴组各12只,用卵蛋白致敏法制作实验性过敏性哮喘模型。经皮给药药贴组予穴位贴敷经皮给药药贴,地塞米松组予腹腔注射地塞米松。结果模型组豚鼠肺组织中MMP-9水平明显高于正常对照组(P0.05),经皮给药药贴组、地塞米松组MMP-9水平均明显低于模型组(P均0.05),均高于正常对照组(P均0.05)。经皮给药药贴组、地塞米松组MMP-9水平无显著性差异(P0.05)。结论穴位贴敷经皮给药药贴可通过降低MMP-9水平,对支气管哮喘产生治疗作用。     

针刺对脑挫伤大鼠脑组织坏死面积及脑细胞凋亡的影响

目的观察针刺对脑挫伤模型大鼠脑组织坏死面积与脑细胞凋亡的影响。方法参照Feeney自由落体撞击造模法建立脑挫伤大鼠模型,将40只大鼠随机分为假手术组、模型组、纳络酮组、针刺组,每组10只。针刺组、纳络酮组分别给予针刺、腹腔注射纳络酮,每天1次,共7天。处理结束后,采用生物医学图像分析系统计算坏死面积、TUNEL法检测脑细胞凋亡。结果模型组大鼠脑组织损伤严重,坏死面积大于损伤面积,出现大量的脑细胞凋亡(P0.01);针刺组、纳络酮组脑组织坏死面积较模型组明显减小,且均小于损伤面积(P0.01);两组大鼠脑细胞凋亡指数较模型组明显减少(P0.01);针刺组脑组织坏死面积与纳络酮组比较无统计学意义(P0.05)。结论针刺可缩小脑挫伤模型大鼠脑织坏死面积、减少脑细胞凋亡,具有确切的抗继发性脑损伤作用。     

针刺对幼鼠HIBD脑组织发育及NGF蛋白表达的实验研究(1)

目的探讨针刺对新生大鼠缺氧缺血性脑损伤(HIBD)脑组织重量及NGF表达的作用及其机制。方法选用85只7日龄清洁级SD大鼠,将大鼠结扎左侧颈总动脉后恢复2h,置于透明密闭容器中,并入37℃恒温水中以1L/min的速度通入低氧气体,2.5h后将动物取出,将存活者继续保温1h时后作行为测定,翻身不能、平衡异常或左旋者视为成功的模型。共72只,随机分为3组,A组为HIBD 针刺Ⅰ组,B组为HIBD 针刺Ⅱ组,C组为HIBD对照组。另设假手术组D组,每组24只。选用幼鼠百会、患侧颞Ⅰ针、内关、曲池、足三里、涌泉,A组于造模后24h开始针刺,前7d仅针四肢部穴位,第8d开始加针头部穴位,B组造模后第8d开始针刺,头、体针同步。针刺均为1次/d,A组连续针刺20d,B组连续针刺13d,假手术组干预措施与A组相同,至HIBD后第21d处死取材,测量各组脑组织重量,采用免疫组化方法测定针刺对HIBD大鼠海马区NGF表达,用图像分析仪在光镜下计算及NGF阳性表达的细胞数目。结果①动物模型的存活率、前肢触觉刺激试验和脑重量的变化结果,都是针刺组优于对照组(P<0.01);②4组左侧海马CA1区神经元NGF阳性数差异有统计学意义,其中D相似文献   

Depressive Neurosis Treated by Acupuncture for Regulating the Liver -A Report of 176 Cases

Objective： To observe therapeutic effect of acupuncture for regulating the liver on depressive neurosis. Methods： In a multi-center randomized controlled trial, 440 patients were divided into 3 groups： Acupuncture group for regulating the liver （Acup., 176 cases） was treated by acupuncture at Siguan Points, i.e. bilateral Hegu （LI 4） and Taichong （LR 3）, Baihui （GV 20） and Yintang （EX-HN3） plus ear-acupuncture, Prozac group （P., 176 cases） by oral administration of Prozac, and Non-acupoint needling group （NAN, 88 cases） by acupuncture at non-acupoints as acupuncture placebo. Self-rating Depression Scale （SDS） was examined before treatment, and one month, two and three months after treatment respectively to evaluate therapeutic effect, and Rating Scale for Side Effects （SERS） was used to evaluate the safety. Results： After one month of treatment, SDS scores in Acup. Group were significantly lower than that in P. Group （P〈0.05） and than that in NAN Group （P〈0.01）, and SDS scores in P. Group were lower than that in NAN Group （P〈0.05）, showing the SDS scores in Acup. Group 〈P. Group 〈NAN Group. After 2 months of treatment, SDS scores in Acup. Group were also significantly lower than that in P. Group （P〈0.01） and than that in NAN Group （P〈0.01）, and SDS scores in P. Group were also lower than that in NAN Group （P〈0.05）, showing the SDS scores in Acup. Group 〈P. Group 〈NAN Group. After 3 months of treatment, SDS scores in Acup. Group were also significantly lower than that in P. Group （P〈0.01） and than that in NAN Group （P〈0.01）, and SDS scores in P. Group were also lower than that in NAN Group （P〈0.01）, showing the SDS score in Acup. Group 〈P. Group 〈NAN Group. After treatment, SERS scores were 0.16±0.95, 6.51±5.09 and 0.23±1.36 in Acup. Group, P. Group and NAN Group respectively. A significant difference existed between Acup. Group and P. Group （P〈0.05）, but no significant difference between Acup. Group and NAN Group （P〉0.05）, showing the SERS scores in Acup. Group 〈NAN Group 〈P. Group. No side effect was found in Acup. and NAN groups. Conclusion： The therapeutic effect of acupuncture on depressive neurosis is better than or similar to that of Prozac but with less side effect.     

捏脊结合针刺对糖尿病胃轻瘫新西兰兔血管活性肠肽含量的影响

目的:探讨捏脊结合针刺对糖尿病胃轻瘫新西兰兔胃窦和近端结肠组织中的血管活性肠肽含量的影响,进而探讨其对缓解DGP症状的作用机制.方法:综合采用链脲佐菌素腹腔注射、高热量饲料不规则喂养和熟地高渗溶液灌胃制备糖尿病胃轻瘫兔模型,将实验新西兰兔分为正常对照组、模型组、捏脊组、针刺组、捏脊针刺组、西沙必利组,经要求治疗2周后采用放射免疫法测定其胃窦和近端结肠组织中血管活性肠肽的含量.结果:与正常对照组比较,组织中血管活性肠肽的含量均下降(P<0.01);与模型组比较,治疗组的血管活性肠肽的含量显著回升(P<0.05),具有统计学意义;四个治疗组之间两两比较,血管活性肠肽的含量无显著差异(P>0.05).结论:捏脊结合针刺能促进糖尿病胃轻瘫新西兰兔胃窦和近端结肠组织中血管活性肠肽的分泌,加快恢复胃肠动力,从而起到降血糖和促进胃肠排空的作用.