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41.
XIA Chun XU Guangxian LIN Changyou HU Tuanjun YAN Ruoqian George F GAO 《自然科学进展(英文版)》2004,14(5):403-410
In order to clarify the molecular sequences,allelic polymorphism and the tertiary structure of grass carp (Ctenophayngodon idellus) MHC class I,and to further study their relationship with disease resistances,grass carp MHC class I gene (Ctid-MHC I) was cloned from a cDNA library and the allelic polymorphism in the population was investigated.The results showed that most of the variations exist in the peptide-binding domain (PBD) and high polymorphism was identified in the Ctid-MHC I allelic genes from 12 individuals.Based on the genetic distance,Ctid-MHC class I can be classified into 6 types (from Ctid-MHC I-UA to Ctid-MHC I-UF) which were subdivided into 9 lineages (from A to I).Comparison of the Ctid-MHC I among animals and humans showed that the key amino acids of the peptide binding sites are conserved.Analysis of the tertiary structure of the PBD between Grass carp and human crystallographic data of HLA-A2,the variation with insertion or deletion was found in eight regions (A~H).The phylogenetic tree of MHC class I indicates the evolution of MHC class I among grass carp,fish,amphibian,birds,higher vertebrates and humans. 相似文献
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Construction of a genetic map with SRAP markers and localization of the gene responsible for the first-flower-node trait in cucumber (Cucumis sativus L.) 总被引:13,自引:1,他引:13
Cucumber (Cucumis sativus L.) is an annualclimber originated from the tropic rain forest area inthe south of Himalayas, which belongs to the Cucur bitaceae family. Cucumber is one of the importantvegetables in the world, and its planting area is sec ond only to that of tomato[1]. However genetics re search on cucumber obviously drops behind that of thelatter. The classic genetic map of cucumber, with sixlinkage groups, is composed of more than 100 genesfor color, morphology, and dise… 相似文献
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With an F2 population from the cross of two cucumber inbred lines, S06 and S52, sequence-related amplified polymorphism (SRAP) was used to construct a genetic linkage map in cucumber (Cucumis sativus L.). Sixty-four SRAP primer combinations generated 108 polymorphic bands in the F2 population analysis. The average of polymorphic bands produced by one primer pair was 1.5, and the maximum was 5. Using Mapmaker 3.0, a linkage map was constructed, which consisted of 77 SRAP markers distributed in nine linkage groups (LOD≥3.0) and spanned 1114.2 cM with an average interval of 14.5 cM between markers. The gene for the first-flower-node trait, termed ffn, was mapped to linkage group Ⅸ, flanked by DC1EM5 and ME7EM2A at 10.3 cM and 12.1 cM distance, respectively. 相似文献
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Introduction In March 2003, a novel coronavirus (CoV) was dis-covered in association with the outbreak of severe acute respiratory syndrome (SARS)[1-3]. The complete genome sequence of several SARS-CoV isolates was soon determined and characterized[4,5]. Comparison of variant SARS-CoV genome sequences has identified certain genetic signatures that can be used to trace sources of infection[6]. Vaccines are now being devel-oped and molecular modeling has suggested that modi-fied rhinovir… 相似文献
47.
麦洼牦牛和犏牛乳蛋白的遗传多态性 总被引:5,自引:2,他引:5
用聚丙烯酰胺凝胶电泳分析了54头麦洼牦牛和19头犏牛乳蛋白的遗传多态性.结果显示,牦牛和犏牛的α乳清蛋白均呈BB型.β乳球蛋白在犏牛中为AB型,在牦牛中A型β乳球蛋白(βLg)在凝胶上染色通常极弱.另外,在7头处于干乳期犏牛的乳腺分泌物中,有5头其A型βLg的电泳迁移率显著快于其它犏牛的A型βLg,而其B型βLg则慢于牦牛和其它犏牛的B型βLg.牦牛和犏牛的β酪蛋白以A1A1型为主,αs1酪蛋白以BB型为主.试验还观察到犏牛的A型αs1酪蛋白电泳迁移率快于牦牛的A型αs1酪蛋白 相似文献
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不同品种果蝇及其杂交后代的RAPD多态性分析 总被引:9,自引:1,他引:8
通过果蝇近交系的建立,用RAPD技术研究了不同品种果蝇及其杂交后代的DNA多态性。结果表明:(1)近交作为一种育种方法,只要利用得当,得是大于害的,在畜禽育种的某些条件下还是可以采用的;(2)不同品种或系间有特异性的RAPD带;(3)在近交系与它们的杂交后代间的RAPD带中,多数带为正反交子代与双亲均相同或正反交子代与单亲共有的带,但有些带上亲代与子代间存在着擀和量的差异,在质的差异上出现双亲中没 相似文献
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杜扬 《贵州大学学报(自然科学版)》1999,16(4):314-318
随着计算机技术的不断发展 ,为了建立、扩展和维护庞大而复杂的软件系统 ,面向对象的程序设计以其具有的封装性、继承性、多态性而成为编程语言的标准 .本文就C 的多态性 ,讨论了重载在编程中的应用 ,并通过具体的实例 ,体现了重载给编程带来的好处 相似文献
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