高效液相色谱法测定通经活络片中青藤碱的含量

[目的]建立通经活络片中青藤碱的质量控制方法.[方法]色谱柱为Kromasil C18色谱柱(250 mm×4.6 mm,5μm),甲醇为流动相A,体积分数0.05％二乙胺为流动相B,体积比为57∶43,流速为1 mL/min,柱温为25℃,检测波长为262nm,进样量15 μL.[结果]青藤碱含量在0.612 ～ 4.08 μg范围内,与峰面积呈良好的线性关系,线性关系良好(r2=0.999 5);平均回收率99.97％.[结论]本研究方法简便、准确、灵敏度高、重复性好,可用于通经活络片中青藤碱的含量测定.     

高效液相色谱法测定花生红衣中儿茶素的含量

目的：建立高效液相色谱法测定花生红衣中儿茶素含量的方法.方法：色谱柱为Agilent HC-C18柱（4.6 mm×250 mm,5 μm）;流动相为水-甲醇-乙腈-冰醋酸（90∶5∶5∶0.4）,检测波长为280nm,流速为0.8 ml/min.结果：线性范围0.09817～3.92688 μg,r=0.9999,平均加样回收率为99.40％,RSD=1.37％.结论：高效液相色谱法简便快速、准确性和重复性好,可用于花生红衣中儿茶素的含量测定.     

高效液相色谱法测定人血清中氨苄西林、丙磺舒的浓度研究

目的:建立测定人血清中氨苄西林、丙磺舒浓度的高效液相色谱法。方法:(1)氨苄西林,以诺氟沙星为内标,血清样品用0.6%的高氯酸沉淀蛋白后进行测定;色谱柱为HigginsTMC18柱(250 mm×4.6 mm,5μm);流动相为有机相(甲醇-乙腈-异丙醇,100∶2∶5,v/v):水相(0.01 mol/L磷酸二氢钾,pH3.5)=23∶77;检测波长210 nm;流速1.0 ml/min;柱温30℃。(2)丙磺舒,以缬沙坦为内标,血清样品用乙腈沉淀蛋白后进行测定;色谱柱为HigginsTMC18柱(250 mm×4.6 mm,5μm);流动相为有机相(甲醇-乙腈,400∶275,v/v):水相(乙酸-水,1∶60,v/v)=77∶23;检测波长280 nm;流速1.0 ml/min;柱温30℃。结果:氨苄西林在0.14~11.2μg/ml浓度范围内线性关系良好(r=0.999 5),最低检测限为0.14μg/ml;丙磺舒在0.20~16.0μg/ml浓度范围内线性关系良好(r=0.999 1),最低检测限为0.20μg/ml。结论:高效液相色谱法操作简便、灵敏、专一性好,适用于人体药动学研究。     

银翘解毒丸（大蜜丸）中牛蒡苷含量的测定

目的建立以高效液相色谱法（RP-HPLC）测定银翘解毒丸中牛蒡苷含量的方法,为其质量控制提供快速、有效的分析手段。方法色谱柱为岛津C18（250 mm×4.6 mm,编号：0032140）色谱柱,柱温30℃,流动相为甲醇∶水（47∶53）,流速1 mL.min-1,进样量10μL,检测波长280 nm,用外标法考察了在该色谱条件下药物的色谱峰,同时进行了专属性、线性关系、精密度等项的考察。结果牛蒡苷在0.808～2.828μg线性范围内与吸收峰面积有良好的线性关系,r=0.999 5,平均回收率为100.2%、RSD为2.82%。结论该方法专属性强,重现性好,且简便、可靠,可用于银翘解毒丸（大蜜丸）中牛蒡苷的含量测定。     

HPLC法测定痛风胶囊中青藤碱和大黄酸的含量

目的建立痛风胶囊中青藤碱、大黄酸含量的测定方法。方法采用HPLC法,青藤碱含量测定的色谱柱为Agilent Eclipse Plus C18(5μm,4.6 mm×250 mm),流动相为乙腈-0.08%(体积分数)三乙胺(体积比21∶79),流速为1.0 mL.min-1,检测波长为262 nm;大黄酸含量测定的色谱柱为Kromasil C18(4.6mm×250 mm,5μm),流动相为甲醇-0.05%(体积分数)H3PO4(体积比85∶15),流速为1.0 mL.min-1,检测波长为254 nm。结果青藤碱在0.115 6～3.855μg范围内与峰面积呈良好的线性关系(r=0.999 5),平均回收率为99.48%,RSD为1.93%;大黄酸在0.083 2～0.832μg范围内与峰面积呈良好的线性关系(r=0.999 5),平均回收率为98.62%,RSD为1.13%。结论本方法专属性强、准确、重复性好,可用于痛风胶囊的质量控制。     

HPLC法测定山绿茶中芦丁的含量

目的:建立山绿茶中芦丁含量的测定方法.方法:采用ODS C18柱,5μm,4.6 mm×250 mm;以乙腈-体积分数2.5％的醋酸溶液(15∶85)为流动相;流速1.0 mL/min;柱温30℃;检测波长254 nm.结果:芦丁在0.01833～0.18330 g/L范围内与峰面积呈良好的线性关系(r=0.9998...     

高效液相色谱法测定利胆排石片中黄芩苷的含量

目的 建立高效液相色谱(HPLC)法测定利胆排石片中黄芩苷的含量.方法 采用色谱柱为Chromanalysis ODS柱(4.6 mm×250mm,5μm)；流动相为甲醇-水-冰醋酸(48∶52∶1)；检测波长278 nm;柱温20℃；流速1.0 mL/min.结果 黄芩苷在2.55～20.4 μg/mL的范围内浓度对峰面积具有良好的线性关系.黄芩苷平均回收率为100.1％,RSD为1.47％.结论 建立的HPLC法操作简便、灵敏度高、精密度好,可为利胆排石片的质量控制提供依据.     

HPLC法测定红霉素明胶微球的含量

目的 建立红霉素明胶微球中红霉素含量的测定方法.方法 采用高效液相色谱法,色谱柱为DIKMA C18色谱柱(5 μm,250 mm×4.6 mm),流动相为0.1 mol/L磷酸二氢铵(三乙胺调节至pH6.5) 乙腈(体积比67∶33),流速1 mL/min;检测波长210 nm.结果 红霉素质量浓度在0.099 2～0.496 0 mg/mL范围内与峰面积呈良好线性关系,r=0.999 9;平均回收率为100 1%,RSD=0.95%(n=9).结论 本文方法可用于红霉素明胶微球的含量检测.     

HPLC法测定小半夏汤中6-姜酚和6-姜醇

目的 研究以HPLC法同时测定小半夏汤中6-姜酚、6-姜醇的方法。方法采用Kromasil C18柱(250 mm×4.6 mm， 5 μm)；流动相：甲醇水(65∶35)；检测波长：280 nm；柱温：30 ℃；体积流量：0.8 mL/min。结果6-姜酚在1.273～11.457 μg与峰面积呈现良好的线性关系， r=0.999 9；6-姜醇在0.444～3.996 μg与峰面积呈现良好的线性关系， r=0. 999 8。平均加样回收率(n=5)：6-姜酚为99.82%；6-姜醇为100.30%。结论本法操作简便，结果可靠，重现性好，可作为小半夏汤质量控制的方法。     

HPLC 法同时测定复方三黄酊中黄芩苷及盐酸小檗碱的含量

目的：建立测定复方三黄酊中2组分含量的高效液相色谱法。方法色谱柱：Thermo,ODS-2HYPERSIL,Dim（ mm）250＆#215;4.6。流动相：甲醇-乙腈-0.025 mol/L磷酸溶液（用三乙胺调节pH为3.0）（15：15：70）;流速：1．0ml/min;检测器：DAD检测器;检测波长：280 nm和345 nm;柱温：25℃。结果黄芩苷在0.06862 ug～0.6862 ug之间进样量与峰面积积分值呈良好的线性关系,平均加样回收率为99.42％, RSD＝0.4％（ n＝6）;盐酸小檗碱在0.0198～0.198 ug之间进样量与峰面积积分值呈良好的线性关系,平均加样回收率100.6％,RSD＝1.9％（ n＝6）。结论本法简单易行,可用于复方三黄酊的含量测定。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.