体外培养条件下LAK细胞对瘤细胞的杀伤效应——扫描电镜及冷蚀…

应用扫描电镜和冷冻蚀刻电镜法观察了体外培养中ＬＡＫ细胞及对Ｒａｊｉ瘤细胞的杀伤攻击现象。文中首先描述了ＬＡＫ细胞及Ｒａｊｉ瘤细胞的超微形态，并重点探讨了对瘤细胞的杀伤效应。作者认为杀伤过程可分为两步：第一步系ＬＡＫ细胞主动性识别靠拢和贴附包围瘤细胞，第二步系攻击效应，攻击杀伤时主要表现为ＬＡＫ细胞以胞吐分泌方式对靶细胞进行穿凿性降解，导致靶细胞胞体空间形成或贯通性损伤，最终引起靶细胞胞浆相泡而死。     

人胚脾LAK细胞体外抗肿瘤作用的实验研究

本文对人胚脾ＬＡＫ细胞的制备及其体外抗肿瘤作用进行了实验研究，为临床推广应用提供了可行性的依据。实验表明，在红细胞低渗休克法，溶血盐法，淋巴细胞分离液分离法中，以最后一种方法制备人胚脾ＬＡＫ前体细胞的效果最佳。用重组白细胞介素２诱导的人胚脾ＬＡＫ细胞在培养的第５～８ｄ对Ｋ５６２靶细胞的细胞毒活性最高，达６５．１％；而对Ｒａｊｉ靶细胞的细胞毒活性较低，在培养的第５ｄ为２６．７％。由此可见，人胚脾ＬＡＫ细胞对Ｋ５６２和Ｒａｊｉ两种肿瘤细胞均有杀伤活性。     

原发性卵巢癌患者CD3AK细胞和LAK细胞杀瘤活性比较

为探讨ＣＤ３ＡＫ细胞和ＬＡＫ细胞杀瘤作用的特异性及靶细胞选择性，对比观察了３例原发性卵巢癌患者ＣＤ３ＡＫ细胞和ＬＡＫ细胞对卵巢癌细胞系和人早幼粒细胞白血病细胞系的杀伤活性。结果表明；ＬＡＫ细胞在培养第１－３周，各效靶比对ＨＬ６０的杀伤活性略高于对３ＡＯ的杀伤活性，但差异无显著性；ＣＤ３ＡＫ细胞在培养第１周，各效靶比对ＨＬ６０的钉伤活性均显著高于对３ＡＯ的杀伤活性，而培养第２，３，４周时，对ＨＬ６０     

正常鼠和免疫鼠CD3AK细胞和LAK细胞杀伤活性的比较研究

实验取正常鼠和Ｐ８１５肿瘤细胞致敏的免疫鼠脾细胞，经抗ＣＤ３抗体与低剂量ＩＬ－２或高剂量ＩＬ－２分别诱生为ＣＤ３ＡＫ细胞和ＬＡＫ细胞，用改良的乳酸脱氢酶释放法以Ｐ８１５细胞和ＹＡＣ－１细胞为靶细胞，测定ＣＤ３ＡＫ和ＬＡＫ细胞的杀伤活性。结果表明：正常鼠ＣＤ３ＡＫ细胞对Ｐ８１５细胞的杀伤活性高于ＬＡＫ细胞，对ＹＡＣ－１细胞的杀伤活性低于ＬＡＫ细胞。免疫鼠ＬＡＫ细胞对Ｐ８１５细胞的杀伤活性明显增高，高     

LAK细胞对有丝分裂期肿瘤细胞的杀伤作用

为了提高淋巴因子激活的杀伤细胞（ＬＡＫ细胞）临床应用的疗效，采用抗肿瘤化疗药物长春新碱诱导人肺腺癌细胞Ａｎｉｐ９７３进入有丝分裂期，研究了ＬＡＫ细胞对有丝分裂期肿瘤细胞的杀伤作用。结果表明，肿瘤细胞进入有丝分裂期后，形态学上发生了明显的变化，ＬＡＫ细胞对有丝分裂期肿瘤细胞的杀伤作用明显增高。电镜下，ＬＡＫ细胞与有丝分裂期的肿瘤细胞以绒毛相接触，呈犬牙交错状，靶细胞的死亡形式是溶解坏死和凋落。     

多抗甲素对人NK细胞活性的作用机理研究

作者应用单细胞细胞毒试验及形态学观察，研究多抗甲素（ＰＡＡ）对人体自然杀伤（ＮＫ）细胞活性的作用机理。结果表明：ＰＡＡ能增强食道癌患者淋巴细胞（ＰＢＬ）明显低下的结合率（Ｂ％）和杀伤率（Ｋ％），也可增强正常人ＰＢＬ的Ｋ％，但对正常人ＰＢＬ的Ｂ％无影响。形态学观察发现：与靶细胞结合的ＰＢＬ除大颗粒ＰＢＬ外，尚有普通的非颗粒ＰＢＬ，结合的靶细胞损伤首先表现为线粒体肿胀、空泡变，然后核固缩，核膜损伤，细胞严重空泡变性等，偶见靶细胞与自然杀伤细胞结合部质膜破损的现象。经ＰＡＡ预处理的靶效细胞结合的形态改变无明显差异，但食道癌组的杀伤作用出现较早，且明显增强。     

雌二醇及他莫昔芬对LAK细胞杀伤卵巢癌细胞作用的影响

目的：探讨雌二醇（Ｅ２）和他莫昔芬（ＴＡＭ）对淋巴因子激活的杀伤细胞（ＬＡＫ细胞）杀伤卵巢癌细胞３ＡＯ作用的影响。方法：分别用Ｅ２,ＴＡＭ,Ｅ２＋ＴＡＭ预处理ＬＡＫ细胞及３ＡＯ细胞１２ｈ,然后以５：１效靶比将效、靶细胞混合作用４ｈ,用流式细胞术检测ＬＡＫ细胞杀伤活性,比较用药前后杀伤活性的变化。结果：Ｅ２预处理ＬＡＫ细胞及３ＡＯ细胞后,其杀伤活性提高了５６．６％～１９９．２％;ＴＡＭ处理后提高了４     

采用MTT方法测定小鼠抗癌效应细胞的细胞毒反应

本文成功地应用ＭＴＴ方法检测抗癌效应细胞的细胞毒反应，确定了ＣＴＬ、ＮＫ和ＬＡＫ细胞的杀伤反应条件，认为选择１０＾５／ｍｌ数量级作为靶细胞密度，杀伤时间为２４小时，效靶比在２０：１或１０：１较为合适。     

LAK细胞及其冻融液,培养上清液对肿瘤细胞的杀伤作用

目的研究ＬＡＫ细胞的活性诱导和杀伤机制。方法用ＭＴＴ法检测不同培养时间的ＬＡＫ细胞、ＬＡＫ细胞冻融液及培养上清液对肿瘤细胞杀伤作用的相关性以及对肿瘤的杀伤作用。方法加入终浓度为１ｍｇ／Ｌ的放线菌素Ｄ以阻断检测过程中肿瘤增殖对实验结果影响。结果①ＬＡＫ细胞冻融液和培养上清液的杀伤活性与ＬＡＫ细胞活性呈正相关（ｒ分别为０．９９６和０．９９９，直线相关性ｔ检验Ｐ＜０．００１和０．００５）；②动态杀伤分析显示，ＬＡＫ细胞动态杀伤活性与ＬＡＫ细胞冻融液、培养上清液之间不呈直线性相关（ｒ分别为０．８４７，０．７９９，Ｐ值均＞０．０５），而ＬＡＫ细胞冻融液和培养上清液间则呈显著直线相关（ｒ为０．９６９，Ｐ＜０．０１）。这表明ＬＡＫ细胞对肿瘤细胞的杀伤方式为快速杀伤，而ＬＡＫ细胞冻融液和培养上清液对肿瘤细胞的杀伤方式则为慢杀伤，ＬＡＫ细胞杀伤活性明显高于ＬＡＫ细胞冻融液。结论ＬＡＫ细胞活性诱导与胞浆内细胞毒因子表达有关；ＬＡＫ细胞可能通过释放细胞毒因子对肿瘤细胞起直接杀伤作用；ＬＡＫ细胞通过与肿瘤细胞直接接触释放杀伤介质，而导致肿瘤细胞凋亡，是其高效、快速杀伤肿瘤细胞的作用机制。     

妇科肿瘤患者CD3AK细胞和LAK细胞杀瘤活性比较

为比较ＣＤ３ＡＫ细胞和ＬＡＫ细胞的杀瘤特性，观察发妇科肿瘤患者ＣＤ３ＡＫ细胞和ＬＡＫ细胞对卵巢癌细胞系的杀伤活性。结果表明；良性肿瘤组ＬＡＫ细胞杀瘤活性高峰在培养第１，２周，显著高于同期培养的ＣＤ３ＡＫ细胞，第３周则较低，而ＣＤ３ＡＫ细胞的杀瘤活性高峰在培养第３，４周，显著高于ＬＡＫ细胞；恶性肿瘤组，ＬＡＫ细胞杀瘤高峰在培养第１周，显著高于同期培养的ＣＤ３ＡＫ细胞，而ＣＤ３ＡＫ细胞杀瘤高峰在培养第     

尖海龙几种不同提取物抗癌作用的比较

将尖海龙用水、磷酸缓冲液、40％乙醇、水煎4种不同方法进行提取,再将4种不同提取物分别作用于Hela、GLT-82、SK-RB-3细胞系,于加药后1d、2d、3d倒置显微镜观察细胞的形态变化,四种提取物3种肿瘤细胞系均有杀伤,且随加药浓度的增加、时间的延长,杀伤效力增强。加药后3d,用MTT法测定细胞的存活率,几种提取方法结果相差不大,对海龙的有效抗癌成分仍不能进行准确的定位,推测海龙的抗癌成分可能不是大分子.也不是脂溶性物质,可能是水溶性的小分子物质。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.