结缔组织生长因子诱导人肾小管上皮细胞向间充质细胞转化的实验研究

Objective To investigate the role of connective tissue growth factor ( CTGF) in epithelial mesenchymal transition of HK-2 cells in vitro.Methods HK-2 cells were randomly divided into two groups; (1) control group including cells cultured in DMEM medium supplemented with 10% fetal bovine serum only; and (2) experimental group including cells cultured in DMEM medium supplemented with 10% fetal bovine serum and recombinant CTGF at a final concentration of 5 p-g/L The cells were collected at 72 h time points.Direct immunoiluorescence staining and immunohistochemistry were used to evaluate the E-cadherin,Vimentin,α-SMA and ERK2 in cells.Western-blotting was used to detect the E-cadherin,Vimentin and ERK2 protein expression.Boyden Chamber was used to detect the migration of tubular endothelium at 1 d,3 d and S d.Results There were less E-cadherin but more Vimentin expressed in cells of the experimental group.The presence of α-SMA was detected at 48 h with peak at 72 h in the cells of the experimental group.On the first day,the cellular migration in the two groups showed no difference.However,after 3 days,the transformed cells migrated surpassed the control group with peak at the 5th day [ (45.0±1.1) : (14.0±1.2),P < 0.05 ) ].Conclusion Connective tissue growth factor induces mesenchymal transformation of HK-2 cells,in which the ERK2 signaling pathway may play an important role.     

结缔组织生长因子诱导人肾小管上皮细胞向间充质细胞转化的实验研究

Objective To investigate the role of connective tissue growth factor ( CTGF) in epithelial mesenchymal transition of HK-2 cells in vitro.Methods HK-2 cells were randomly divided into two groups; (1) control group including cells cultured in DMEM medium supplemented with 10% fetal bovine serum only; and (2) experimental group including cells cultured in DMEM medium supplemented with 10% fetal bovine serum and recombinant CTGF at a final concentration of 5 p-g/L The cells were collected at 72 h time points.Direct immunoiluorescence staining and immunohistochemistry were used to evaluate the E-cadherin,Vimentin,α-SMA and ERK2 in cells.Western-blotting was used to detect the E-cadherin,Vimentin and ERK2 protein expression.Boyden Chamber was used to detect the migration of tubular endothelium at 1 d,3 d and S d.Results There were less E-cadherin but more Vimentin expressed in cells of the experimental group.The presence of α-SMA was detected at 48 h with peak at 72 h in the cells of the experimental group.On the first day,the cellular migration in the two groups showed no difference.However,after 3 days,the transformed cells migrated surpassed the control group with peak at the 5th day [ (45.0±1.1) : (14.0±1.2),P < 0.05 ) ].Conclusion Connective tissue growth factor induces mesenchymal transformation of HK-2 cells,in which the ERK2 signaling pathway may play an important role.     

反义细胞外信号调节激酶对移植物血管的保护作用

目的探讨反义细胞外信号调节激酶（ERK1／2）基因治疗对移植物血管的保护作用及可能的保护机制。方法建立BN至Lewis大鼠的腹主动脉移植模型。反义ERK1／2治疗组移植前取供者动脉血管段给予经脂质体包装的反义ERK1／2基因转染；腹主动脉移植术后1个月内受者每日从尾静脉或阴茎背静脉注入经脂质体包装的反义ERK1／2寡核苷酸100μl。对照组移植未经任何处理的血管段，移植后也无特殊处理。移植术后60d取移植段主动脉进行组织病理学观察内膜和胶原纤维变化；免疫组织化学法观察移植段血管ERK1／2基因的表达和CD4^＋、CD8^＋T淋巴细胞的浸润情况；ELISA法检测血清中细胞间粘附分子（ICAM-1）的变化。结果移植术后60d，对照组的移植动脉呈慢性移植物血管病表现，血管内膜显著增厚，移植血管中ERK1／2基因高表达，CD4^＋、CD8^＋T淋巴细胞大量浸润；反义ERK1／2基因治疗组移植动脉呈血管内膜炎改变，ERK1／2基因表达不明显，内膜有少量CD4^＋、CD8^＋T淋巴细胞；对照组ICAM-1表达显著高于反义ERK1／2治疗组（P〈0．05）。结论反义ERK1／2基因治疗对移植物血管具有保护作用，可以减缓慢性移植物血管病的发生，这种保护机制可能和减少ICAM-1的表达以及减少移植血管T淋巴细胞的浸润有关。     

脾移植治疗血友病甲长期疗效观察

脾移植治疗血友病甲长期疗效观察同济医科大学器官移植研究所（４３００３０）陈如水，夏穗生，刘乐欣，于昌松姜洪池现在哈尔滨医科大学一院周晓霞，王滔，曾凡军，刘慎微，刘教贵，徐慧珍血友病甲是最常见的遗传性出血性疾病，过去无根治方法，仅靠补充外源性第Ⅷ因于或...     

超声下细针插入引导肝切除

术中超声常用于肝脏切除的定位以使切除边缘有足够范围的无瘤区。这需要重复使用术中超声而致手术时间的延长。本文介绍了一种新的简单的方法,即在超声引导下向肝脏插入细针,再沿细针行肝切除术。技术:日本Aloka—SSD256型实时线阵显像仪,5MHz消毒探头用作术中超声。直接将探头置于肝脏表面检查肿瘤与肝内血管的解剖关系。在超声引导下从供应肿瘤营养的门脉分支结扎处的远端穿刺和注入     

神经降压素基因在大鼠生长发育各期肝脏上的表达

神经降压素(NT)是一种位于成年脑组织和空回肠粘膜内皮细胞上的三十多肽,是调节肠道运动、分泌以及小肠和结肠粘膜生长的十分重要的激素。编码NT基因(同时编码neuromedin N,即NT/N基因)的结构最近已在大鼠、犬和人体中测定出来。NT/N基因和NT多肽除在正常肠组织表达外,还在许多胃肠道恶性肿瘤中异常表达。例如,NT/N并不在正常成年肝脏表达,但在肝细胞癌的一种变型——纤维板层肝癌中可见表达。后者的特点是α-FP阴性和vitB_1~2高粘合力,且预后比典型的肝细胞较好。本研究的目     

既往不能切除的肝肿瘤体外肝切除

深部和邻近大血管的肝肿瘤,如位于肝蒂门静脉分叉处和肝静脉进入下腔静脉(IVC)汇合处肝肿瘤的切除,由于存在着不可控制的大血管出血的可能危险,故可治性显著减少。近年原位肝移植的进展,无肝期静脉转流的应用,低温肝脏保存的安全延长,可行原     

亲属脾移植术后移植物抗宿主反应和移植脾亢的成功治疗经验

移植物抗宿主反应（ＧＶＨＲ）和移植脾亢是脾移植的两个棘手问题，因二者来势凶猛，控制困难，既往治疗切除移植脾脏。我们介绍一例你亲供脾移植术后天和２１天发生ＧＶＨＲ和移植脾亢，分别采用深部Ｘ线及钴６０局获得成功的治疗经验。     

同种肾移植术后应用咪唑立宾免疫抑制治疗的临床研究

目的探讨咪唑立宾(MZR)与环孢素A(CsA)和激素组成基础免疫抑制方案在同种肾移植中的有效性与安全性。方法28例首次肾移植患者(男19例,女9例)随机分为2组:MZR组,14例,MZR初始剂量为1、2mg/(kg·d);MMF组,14例,MMF初始剂量采用1.5～2.0g/d。两组均接受CsA与激素联合的基础免疫抑制方案,CsA浓度保持同一水平,随访6个月。结果两组人、肾存活率均为100%,各出现1例次急性排斥反应,术后24周肌酐平均水平,MZR组为(100.2±1.7)μmol/L,MMF组(98.6±2.5)μmol/L,没有显著性差异(P>0.05)。副作用方面,胃肠道不适和白细胞减少等两组类似,而MZR组出现2例高血糖,可能与激素有关。结论MZR用于肾移植术后维持免疫抑制治疗,可成功替代MMF,有效预防急性排斥发生。