过氧乙酸消毒剂的毒性试验

目的：对一元包装过氧乙酸消毒剂的急性和亚急性毒性进行研究,为其安全使用提供依据。方法依据卫生部《消毒技术规范》（2002年版）进行试验：（1）急性毒性试验：选用健康Wistar大鼠60只,随机分组,一次性经口灌胃不同剂量消毒剂,观察大鼠的中毒症状和死亡情况,计算半数致死量（LD50）。（2）亚急性毒性试验：选用健康Wistar大鼠40只,随机分为3个剂量组和阴性对照组,连续经口灌胃（33～342）mg/kg体重消毒剂28d,试验结束后检测大鼠体重、脏/体比值、血液学指标及血清生化指标,并进行病理组织学检查。结果对雌性、雄性大鼠LD50分别为1470mg/kg体重、1710mg/kg体重;大鼠亚急性试验各剂量组体重、血液学指标、生化指标、脏/体比值,与阴性对照组比较,统计学上均差异无显著性;大体解剖观察未见异常,未见与受试物有关的病理组织学改变。结论该过氧乙酸消毒剂对大鼠急性经口毒性为低毒级,且在本试验剂量范围内,未观察到明显的亚急性经口毒性。     

蝙蝠蛾参鹿片亚急性毒性安全性评价

目的研究蝙蝠蛾参鹿片的急性和亚急性毒性,为其食用的安全性提供试验依据。方法急性毒性试验:ICR小鼠雌雄各10只,采用最大耐受剂量法经口灌胃,观察14 d。亚急性毒性试验:Wistar大鼠雌雄各40只,随机分为低、中、高剂量组及对照组4组,每组雌雄各10只,连续喂养30 d,对各剂量组大鼠各项指标进行观察,并对数据进行统计分析。结果急性毒性试验:雌、雄性小鼠MTD 15. 2 g/(kg·BW),属无毒级。亚急性毒性试验:各剂量组与对照组大鼠在试验过程中均生长良好,各剂量组的增重、食物利用率、进食量、脏/体比、血液学指标、血液生化指标等指标与对照组比较,差异无统计学意义,主要器官未见病理改变,未观察到最大有害作用剂量。结论本试验条件下,蝙蝠蛾参鹿片具有较好的安全性。     

盐酸聚六亚甲基胍消毒洗手液的亚急性毒性研究

目的对0．3％盐酸聚六亚甲基胍（PHMB）的消毒洗手液进行了哺乳动物的亚急性毒性试验，以研究消毒剂多次接触对实验动物的蓄积毒性作用及其靶器官，并为亚慢性、慢性毒性或致癌试验的剂量设计提供依据。方法试验依据卫生部的《消毒技术规范》（第2002版）消毒产品毒理实验规范，选用SPF级的sD大鼠，进行大鼠急性经口毒性LD50试验、亚急性毒性试验。结果大鼠急性经口毒性试验ID50〉5000mg/kg·BW，属实际无毒级；亚急性毒性试验，一般生理体征无异常，对大鼠生长曲线无明显影响，血常规指标无异常，生化指标无异常，脏体系数无异常，脏器组织病理学检查未发现异常。结论0．3％盐酸聚六亚甲基胍消毒洗手液为实际无毒级，亚急性试验各指标无异常。     

溪黄草的亚慢性毒性研究

目的研究溪黄草的亚慢性毒性。方法选用80只健康SD大鼠随机分为4组,雌雄各半,分别经口灌胃给予0.000、0.625、1.250、1.875 g/kg剂量的溪黄草浸膏连续90 d,观察动物生长情况,试验中、末期对动物的血液学和血清生化指标进行检测,试验末计算动物主要脏器的脏体比值并进行组织病理学检查。结果试验期间动物无死亡。试验末各剂量组动物体重、增重、进食量、食物利用率、脏器绝对质量及脏体比值与对照组比较,差异无统计学意义(P0.05)。在试验的中、末期各剂量组动物血液学和血清生化检测指标均在本室正常值范围内,与对照组比较差异无统计学意义(P0.05)。大体解剖观察和病理组织学检查也未见与受试物相关的异常改变。其最大未观察到有害作用剂量为1.875 g/kg。结论在本实验室条件下,未观察到溪黄草浸膏对SD大鼠有明显的毒副作用。     

微孔草籽油中药胶囊的30d大鼠喂养试验研究

目的通过青藏高原特有珍稀油料植物微孔草籽油与中成药配伍得到一种具有缓解视疲劳作用的软胶囊,为评价该软胶囊的毒理学安全性,对该样品进行30d大鼠喂养实验。方法将受试样品分别按0.9、1.8、3.6g/Kg.BW剂量(分别相当于人群日摄入量的54、108、216倍)对SPF级Wistar大鼠连续经口灌胃30d,观察大鼠中毒症状和死亡情况。结果各实验组大鼠体重、进食量、食物利用率、血常规、血液生化指标、脏体比及病理组织学观察等各项指标与阴性对照相比较差异均无统计学意义。结论 30d大鼠喂养实验表明该受试样品未发现明显毒性作用。     

辅酶Q10毒理学安全性评价

目的探讨辅酶Q10毒理学安全性。方法采用小鼠急性经口毒性试验,遗传毒性试验（Ames试验、小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸形试验）及大鼠30d喂养试验,分别观察辅酶Q10的急性毒性、遗传毒性和亚急性毒性。结果辅酶Q10对小鼠的最大耐受剂量（MTD）大于30000mg／kg．BW,遗传毒性试验结果均为阴性;以360、540、720mg／kgBW（分别相当于人体推荐用量的50、75、100倍）3个剂量的样品连续给大鼠灌胃30d,试验期间动物生长发育良好,各剂量组大鼠的体重、增重量、进食量、食物利用率、血常规和生化检测指标、脏器重量及脏器／体重比值与对照组比较,差异均无统计学意义（P〉0．05）;组织病理学检查,各实验组大鼠肝、肾、胃肠、脾、睾丸和卵巢等组织均未见有病理改变。结论辅酶Q10对动物无明显毒性。     

苦瓜醇提取物亚急性经口毒性研究

目的评价苦瓜醇提取物的经口亚急性毒性。方法将苦瓜醇提取物按1.25、2.50和5.00g/kg·bw剂量给大鼠连续灌胃30d,测定体重、进食量,计算食物利用率,实验末采血测定血象和血生化指标。结果苦瓜醇提取物各剂量对大鼠体重增长、总进食量及食物利用率无显著影响(P0.05)。与相应对照组比较,雄鼠1.25、2.50g/kg·bw剂量组血肌酐显著降低,雌鼠三个剂量组总蛋白均较对照组降低,其中2.50、5.00g/kg·bw剂量组总蛋白降低有显著性。各剂量对其他血液学、血生化指标、肝脏、脾脏、肾脏和雄鼠睾丸、雌鼠卵巢的脏体比值无显著影响。大体解剖和组织病理检查未见与样品有关的异常改变。结论苦瓜醇提取物给大鼠喂养30d,2.50g/kg·bw以上剂量可引起雌性大鼠总蛋白降低,高达5.00g/kg·bw的剂量未产生其它可观察到的毒副作用。     

益生降糖饮的食用安全性毒理学评价

目的 对几种药食同源物的复合水提成分"益生降糖饮"的食用安全性进行毒理学评价.方法 Wistar大鼠80只,雌雄各半,体重(200±20)g,随机分成4组,包括低、中、高剂量组和对照组.对益生降糖饮进行急性经口毒性试验和30 d喂养试验.每周记录1次体重和2次食物摄入量,计算食物利用率.试验期末,检测大鼠血常规及血清生化指标,并取大鼠主要脏器进行组织病理学观察.结果 益生降糖饮对Wistar大鼠的急性经口LD50>100 mg/kg,是推荐饮用量的200倍以上.30 d喂养试验显示,大鼠一般情况未见明显异常;体重、食物利用率及脏体比与对照组相比,差异均无统计学意义;各剂量组的血常规及血清生化指标检测值均与对照组无明显差别;各剂量组大鼠大体解剖及心、脑、肝、肾脏组织病理学检查未见明显异常.结论 益生降糖饮未观察到明显的急性经口毒性和亚急性毒性,在试验剂量范围内有较高的食用安全性.     

茶树油皮肤消毒液的毒理学安全性评价

目的研究茶树油皮肤消毒液反复使用对哺乳动物的毒性,为茶树油消毒剂的进一步开发利用提供理论依据。方法按照《消毒技术规范〉）（2002年版）,采用大、小鼠急性经口毒性试验、小鼠骨髓嗜多染红细胞微核试验、家兔皮肤刺激性毒性试验和大鼠亚急性毒性试验,观察茶树油皮肤消毒液对实验动物的毒性作用。结果大、小鼠的急性经口毒性试验结果显示,茶树油皮肤消毒液的LD50均大于5000mg／kgBW,属实际无毒级;小鼠骨髓嗜多染红细胞微核试验结果为阴性,未发现该受试样品对体细胞有诱变作用;新西兰家兔的多次完整皮肤束B激实验结果,每只动物的平均积分小于0．5,属无刺激性;亚急性毒性试验,给药量在1000mg／kgBW以下,各剂量组与阴性对照组比较,大鼠的体重、脏体系数、血清生化指标及血常规无显著影响,脏器组织病理学检查未发现异常。结论在本实验条件下,茶树油皮肤消毒液毒性较低,刺激性小,将其开发成消毒剂具有广阔的前景。     

DEHP对子代大鼠血液学变化的影响

目的 观察DEHP对子代大鼠血液学变化的影响.方法成熟Wistar大鼠,在妊娠期7-16d,每天经口灌胃给予DEHP高(500mg/kg)、低(250mg/kg)三剂量组,对照组经口灌胃给予照物玉米油.检测子代大鼠血液学指标.结果 子代大鼠血液学中Hb、RBC、WBC及Lym、Mon、Neu、Eos、Bas指标与对照组相比,差异无统计学意义(P＞0.05).结论 在本次试验中未见到DEHP对子代大鼠血液学变化的明显影响.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.     

Possible Spread of adenovirus type 3 from poultry to humans ：indirect evidence from an outbreak in China

Objective:To explore the epidemiology and etiology for an outbreak of acute respiratory tract infection that occurred in one county of Jiangsu Province, China 2004. Methods: Only cases meeting the case definition were included in the study. We reviewed the medical records of the cases who were admitted to the local hospitals, interviewed cases by a standard questionnaire, and then described the epidemiotogic features and analyzed risk factors by means of a case-control study. We collected pharyngeal swab specimens and sent them to different laboratories for isolation and culture. The laboratory used different detection methods such as DIP, PCR, electron microscope examination and microneutralization assay, to identify and then type the positive specimens. Results:A total of 871 cases were reported during the period from April 18 to July 4,2004. The distribution of onset times presented two peaks, one in late May and another in middle June. The epidemic occurred mainly in the elementary and junior high schools in ten townships of one county, and the mean age of the cases was 12 years (range 7 months to 18 years). The course of the disease was acute, and was characterized by fever accompanied with sore throat and tonsillitis. The WBC count of cases was normal or elevated. The mean duration of illness was 5 days (range 2 to 12 days). No fatalities from illness were reported. A case-control study indicated that the possible risk factors were close contact with a case and/or poultry before onset and sharing of towels among members of the family. The typical CPE was observed through inoculating pharyngeal swab specimens into the HEP-2 cell cultures in different laboratories. An infection of adenovirus type 3 was verified by detecting positive specimens in different methods. Conclusion:This investigation demonstrated that the acute respiratory infection in cases was caused by adenovirus type 3. Cases occurred in over 70 schools in ten townships in 2004, and the route of transmission was possibly close contact with cases or droplet transmission.