炭凝试验诊断猪细颈囊尾蚴病的研究

作者采用炭凝集法诊断活猪细颈囊尾蚴病,证明它是一种简便、快速,适于现场操作的方法。炭凝集诊断所用抗原,经试验筛选,以减压分馏提纯细颈囊尾蚴囊液制作的炭抗原为好,其浓度以1—4为宜,符合率86.6％。该抗原与猪姜片形吸虫、猪蛔虫、猪巨吻棘头虫惠猪溶血进行炭凝试验,无交叉反应,其特异性较强。     

四川省一市两县猪细颈囊尾蚴病流行病学调查研究

四川省成都市、合川县、乐至县猪细颈囊尾蚴的感染率为36.30—82.75％,感染强度为1—264。感染初期蚴虫主要寄生于肝脏,后期主要寄生于网膜;寄生于犬体的细颈囊尾蚴的成虫——泡状带绦虫,感染率为25—38.8％(屠场附近),犬人工感染细颈囊尾蚴后,在第62—64天开始排出成熟孕节,排出孕节的动态呈波浪式并有明显的季节性,以3—5月和9—11月为高峰,严冬和盛夏很少排出;调查的55头阳性猪,因细颈囊尾蚴寄生,平均每头减少肉重0.35kg,足油0.5kg,我省每年出栏生猪约6千万头,按30％感染率计,每年全省将损失2千多万元人氏币。该研究为防治猪细颈囊尾蚴病(感染)提供了科学依据。     

丙硫咪唑治疗猪人工感染细颈囊尾蚴病的试验研究

1990年7月—1991年7月应用丙硫咪唑治疗猪人工感染细颈囊尾病获得成功,这在国内尚属首次。在多种剂型、剂量和投药方法对比试验中,以粉剂90mg/kg,三次口服疗效最好,驱(杀)虫率为99.44—100％,投药方便无副作用。治疗后6—13天蚴虫死亡,死虫14—25天干酪化,外膜上有一薄层纤维膜。26—60天干酪化、纤维化。177天吸收较好。丙硫咪唑是广谱驱虫药且价格较低,是目前治疗猪细颈囊尾蚴病(感染)较理想的药物。该研究为治疗猪细颈囊尾蚴病(感染)提供了科学依据。     

几种寄生虫抗原组分的SDS-PAGE电泳比较分析

通过提取制备猪带绦虫、猪囊尾蚴虫体、猪囊虫囊液、多房型束球绦虫、肝片吸虫、细颈囊尾蚴、细颈囊尾蚴囊液和羊绦虫等8种寄生虫抗原，运用SDS－PAGE电泳法比较各种抗原的蛋白质的组成，不连续SDS－PAGE电泳结果显示猪带绦虫、猪囊尾蚴虫体、猪囊虫囊液、多房示绦虫、肝片吸虫、细颈囊尾蚴、细颈囊尾蚴囊液和羊绦虫等几种抗原的蛋白质在凝胶中分别有31，23，18，17，21，22，8和24条带，分子量在13kD-134kD之间，8种抗原的电泳带之间既有相似性又有特异性，其抗原的共同蛋白质亚基带的分子量为63kD,38kD和24kD。     

利用间接血凝试验检测猪气喘病

本文介绍采用间接血凝反应(IHA)技术检测猪气喘病。试验结果表明,对患有气喘病猪的检出率可达97.7％,对健康猪的检出率达100％。在诊断猪气喘病方面,IHA是一种快速,敏感的检测方法,比目前普遍采用较长时间的观察临床症状的诊断方法,更具有可靠性和实用性。     

猪肉絛蟲的囊尾蚴熱力致死試驗

前言猪肉条虫在我国分布甚广,南北各地均有发现。其感染来源是由于人食入未熟含有囊尾蚴之猪肉而得。因此猪之囊尾蚴感染,为人体猪肉条虫病流行之主要因素;根据上海屠场一年内之统计,囊尾蚴病猪平均发病率为0.2357％,天津为2.5％,青岛市屠场1954年宰猪总数为580971头,囊尾蚴病猪数为2728头,其平均发病率为0.469％。猪囊尾蚴感染率之高低与人类猪肉条虫病的感染有着密切关系。猪肉是我国人民主要的肉食(除个别民族外)。在食用方法上虽各地不同,但在食肉习惯     

猪囊尾蚴病抗体技术研究进展

猪囊尾蚴病每年造成大量的经济损失,抗体技术防治猪囊尾蚴病的出现极大地提高了治疗此病的手段。本文从单克隆抗体、单链抗体两个方面对猪囊尾蚴病防治做了详细的介绍。     

牛羊细粒棘球蚴病现场快速检测方法的建立及应用

建立了一种基于恒温隔绝PCR(Insulated isothermal PCR,ii PCR)技术现场检测牛羊包虫病的方法.结果显示,该方法只能检出细粒棘球绦虫,对多房棘球绦虫、肝片吸虫、细颈囊尾蚴、前后盘吸虫、日本血吸虫、金黄色葡萄球菌、伪结核杆菌无关病原不检出;检测下限为100个拷贝,重复性好.对55份牦牛源包囊样本和70份羊源包囊样本的检出率分别为61.8和4.3%,与文献报道的检测方法的符合率分别为100%;本研究建立的ii PCR方法特异性好、灵敏度高、重复性好,从核酸提取到报告检测结果仅需1小时,操作方便,可现场使用,为牛羊包虫病的现场快速诊断提供有力的工具.     

西安与汉中屠宰场常见的寄生蠕虫

一前言寄生蠕虫对于家畜的危害较为普遍,要想增加畜产质量,提高家畜的役使能力,研究家畜寄生虫病,是很重要的工作之一。几年来我们在西安与汉中屠宰场中采到一些标本,其中吸虫纲五种、绦虫纲四种、线虫纲二种、棘头虫纲一种。在汉中屠宰场采到的有肝片形吸虫、大片形吸虫、胰阔盘吸虫、姜片虫、猪囊虫、细颈囊尾蚴、猪蛔虫等。在西安屠宰场采到的有姜片虫、矛形复腔吸虫、猪囊虫、扩展莫氐绦虫、细颈囊尾蚴、猪蛔虫、捻转血矛线虫及大棘吻棘头虫等。现将这些标本加以整理鉴定,并提出粗浅的建议,供有关部门参考。     

猪带绦虫抗原基因TS76真核表达的研究

将猪囊尾蚴抗原基因TS76亚克隆至VR1020真核表达载体中，用菌浇原位杂交法筛选重组质粒，将其转染Cos7细胞，用Northern Blot鉴定插入片段的mRNA转录，用ELISA检测其表达产物。结果表现：VTS76在真核细胞中能够转录TS76基因；表面产物被兔抗猪囊尾蚴高免血清所识别，在转染真核细胞后24h，即可检测到正确表达的猪囊尾蚴抗原蛋白。     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Wuhan University Journal of Natural Sciences TOTAL CONTENTS

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Advances in broadband wireless communications under high-mobility scenarios

Future mobile communication systems aim at providing very high data transmission rates, even in high-mobility scenarios such as high-speed wheel-track trains, maglev trains, highway vehicles, airplanes, guided missiles or spacecraft. A particularly important commercial application is the strong and increasing worldwide demand for high- speed broadband wireless communications （up to 574.8 km/ h test speeds or 380 km/h commercial speeds） in railways, providing data, voice and video services for applications such as onboard entertainment services to passengers, train control, train dispatch, train sensor status handling and sur- veillance. In such high-mobility scenarios, there are a number of communication challenges, including fast hand- over, location updating, high-speed channel modeling, estimation and equalization, anti-Doppler spreading tech- niques, fast power control, and dedicated network architec- ture. Because signal transmission in very high-speed scenarios will inevitably experience serious deterioration, it is imperative to develop key broadband mobile communi- cation techniques for such high-speed vehicles.     

Perspherical Approaches for Electrons in Atoms and Charged Excitons in Quantum Wells

Instead of following Fock’s expansion,we solve the Schrodinger equation for some quantum mechanical manybody systems such as electrons in atoms and charged excitons in quantum wells in a similar way in hyperspherical coordinates by expanding the wave functions into orthonormal complete basis sets of the hyperspherical hannonics(HHs)of hyperangles and generalized Laguerre polynomials(GLPs)of the hyperradius.This leads the equation to     

Scale-based spatial data model for GIS

Being the primary media of geographical information and the elementary objects manipulated, almost all of maps adopt the layer-based model to represent geographic information in the existent GIS. However, it is difficult to extend the map represented in layer-based model. Furthermore, in Web-Based GIS, It is slow to transmit the spatial data for map viewing. In this paper, for solving the questions above, we have proposed a new method for representing the spatial data. That is scale-based model. In this model we represent maps in three levels: scale-view, block, and spatial object, and organize the maps in a set of map layers, named Scale-View, which associates some given scales.Lastly, a prototype Web-Based GIS using the proposed spatial data representation is described briefly.     

英文诗歌中的喻类修辞

为了有助于提高英文爱好者的个人情操、文化素养和学习兴趣,以及专业人员对英语学习和研究进行多途径的探索,本文通过一些精选诗歌的引证和分析,着重论述了喻类修辞法在英文诗歌中的运用其及效果。     

Man Is Unable to Change the Miserable Life——Analysis of the Arrangement of Four Characters in The Glass Menagerie

Tennessee Williams is considered as one of the most important American playwrights since World War II.The Glass Menagerie is his first successful drama,which describes a tragic situation of family and means to say that Man is unable to change the miserable life,no matter whatever means he try,This essay focuses on the analysis of the arrangement of the four main characters:Laura.Amanda,Jim and Tom to reveal the theme.Laura is fragile.Amanda is brave.Jim is vital.Tom is sensible.And all of them develop and try the different means to struggle against life,but fail tragically.With the evidence,the paper comes to conclusion naturally that Man is unable to change the miserable life,and he dooms to fail.     

Characterization of root-nodulating bacteria on Retama raetam in arid Tunisian soils

The aim of this study is to investigate the diversity of Retama raetam root-nodule bacteria isolated from arid regions of Tunisia. Twelve isolates, chosen as representative for different 16S rRNA gene patterns, were characterized by 16S rRNA gene sequencing and phenotypic analysis. Isolates were assigned to Sinorhizobium, Rhizobium and Agrobacterium. Symbiotic properties of Sinorhizobium and Rhizobium isolates showed a large diversity in their capacity to infect their host plant and fix atmospheric nitrogen. Strain RK 22 identified as Rhizobium was the most effective isolate.     

WIMAX与WI-FI技术分析及联合组网

介绍了WiMAX与Wi-Fi两种无线宽带接入技术,并对两者之间的关系及相互之间的影响做了对比及分析,并对WiMAX的关键技术进行了详细说明,最后对两者的联合组网方式做了简单的探讨。     

An Affordable Accurate Evaluation of the Binding Conformations Predicted by Molecular Docking Studies in Bio-System

正Recently,docking has been widely used to predict the binding-modes of protein-inhibitors,when the crystal complexes structure was absent.Most docking algorithms are able to generate a large number of probable conformations,it,however,is difficult to effectively evaluate these docking poses and identify the most reasonable bindingmode.In the present study,on the basis of the crystallographic data of human 3-hydroxy-3-methylglutaryl coenzyme