芪苈强心胶囊配合保元汤加减治疗慢性心力衰竭临床研究

目的:观察芪苈强心胶囊配合保元汤加减用于慢性心力衰竭的治疗效果。方法:选取收治的慢性心力衰竭患者104例作为研究对象,运用随机数字表法将其分为观察组(52例)和对照组(52例),两组患者均接受常规治疗,对照组采用保元汤治疗,观察组同时应用芪苈强心胶囊联合保元汤治疗。观察患者治疗前及治疗后6个月的6 min步行试验的步行距离(6MWD)及纽约心脏病学会心功能分级(NYHA)、血清学指标N末端B型利尿钠肽原(NT-proBNP)、半乳糖凝集素3(Galectin-3)及白介素6(IL-6)、超敏C反应蛋白(hs-CRP)、肿瘤坏死因子α(TNF-α)、心脏指数(CI)、左室射血分数(LVEF)、左室舒张末期内径(LVEDD)、左室收缩末期内径(LVESD)、左室重量指数(LVMI)和室壁运动指数(WMI)、左室间隔厚度(IVST)变化情况。结果:治疗前后,两组患者比较心功能指标、Galectin-3、炎性因子指标、心室重构指标差异有统计学意义(P<0.05); 观察组与对照组患者相比,6 min步行距离、CI、LVEF、升高更为显著(P<0.05); 观察组患者NYHA分级、NT-proBNP、Galectin-3、IL-6、hs-CRP、TNF-α、LVEDD、LVESD、LVMI、WMI、IVST降低更为显著(P<0.05)。结论:芪苈强心胶囊辅助保元汤加减能够有效改善慢性心力衰竭患者心脏功能,有利于患者体内炎性因子及Galectin-3水平降低,抑制心室重构进展,患者心脏舒缩功能改善效果更佳。     

家兔旁臂内侧核区微量注射羟基马桑毒素所致呼吸效应

实验在44只戊巴比妥钠麻醉、双侧颈迷走神经切断的家兔上进行。一侧旁臂内侧核(NPBM)区微量注射1μ1(10μg)羟基马桑毒素(Tutin)(n=13),均可见呼(Te)、吸(Ti)时程,呼吸频率和膈肌肌电积分幅度改变,最突出的反应是呼吸暂停(n=4)。一侧注射后2～7h仍未出现呼吸停止的动物,在另一侧NPBM注     

四川红原草炭腐植酸钠对猫大脑皮层电图的影响

在清醒、箭毒化麻痹的猫实验,观察了四川红原草炭腐植酸钠对大脑皮层电图的影响。结果发现,小剂量腹腔(15mg/kg)或静脉(10mg/kg)注入,或较大剂量(30—50mg/kg)腹腔注入,在注入后观察的90min内,均未见皮层电图和同步记录的心电图出现明显的改变,但静脉注入较大剂量(15—40mg/kg)则较易出现皮层电图和心电图的异常改变。这与戊巴比妥钠引起的皮层电图改变并不相同。     

用辣根过氧化物酶溶液低温快速充灌玻璃微电极的方法(摘要)

近年来不少人将电生理学方法与神经解剖学方法结合起来研究中枢神经系统。如用充灌有HRP溶液的玻璃电极记录神经细胞的电活动后,再电泳HRP,以了解记录部位神经核团或神经元的功能、形态及神经联系。但是,在常温下用HRP溶液充灌玻璃微电极常在尖端出现气泡,可使记录不稳定或是通过的电流特性不稳定。我们参照Lewis B. Haberly和James M Bower(1981)提出的快速充灌玻璃微电极的方法,结合我们的条件,稍加改进作了一些试验。该法是在低温下进行充灌。其原理是温度降低时,可使表面张力增加,从而     

慢性胃炎病瘀血理论及活血化瘀治疗临床研究

笔者于1968年开始感到慢性胃炎治疗困难，一些铁道兵病人长期住院、经久不愈。从此，笔者回头学习中医中药，试图寻找中西医结合有效方法。于1979年在中华内科杂志上提出的“今后值得研究的几个问题……(5)各型胃炎的中西医结合治疗”的鼓动下，开始运用中医理论探讨本病的病理改变，寻找中西医结合诊治本病的病理学依据。     

家兔臂旁内侧核在“人中”穴呼吸效应中的作用研究

Experiments were performed on 27 rabbits, bivagotomized, gallamine triethiodide immobilized and artificially ventilated. The effects of electric stimulation of "Renzhong" (Du26) area, NPBM separately, and "Renzhong" area and nucleus parabrachialis medialis (NPBM) simultaneously, on phrenic nerve discharge were observed before and after electolytic and chemical lesion of NPBM. Evoked potential (EP) could be recorded in NPBM during the electric stimulation "Renzhong" area. Electric stimulation of the same site of NPBM where the EP was recorded could induce respiratory response and change the effect of electric stimulation of "Renzhong" area on respiration. But after electrolytic and chemical lesion of NPBM, stimulation of NPBM could no longer induce the respiratory response mentioned above and could not change the "Renzhong" effect on respiration either. These results suggest that NPBM might take part in respiratory effects of "Renzhong" area stimulation, but is not essential to it.     

家兔延髓吻端腹外侧区在“人中”穴加压效应中的作用

延髓吻端腹外测区(Rostral ventrolateral medulla,RVL)微量注射红藻氨酸(Kainic acid,KA)前,分别用弱、强电刺激“人中”穴时可引起动脉血压(BP)显著升高,心率(HR)明显增快。一侧或双侧RVL注射KA后,再用弱、强电刺激“人中”穴时,BP、HR的反应基本消失。结果表明,RVL在“人中”穴加压效应中起关键性作用。     

家兔“人中”穴区传入神经的中枢投射：CB—HRP跨神经节追踪法研究

实验选用成年家兔9只,将2%CB-HRP5微升注入“人中”穴区,其中4只事先切断一侧眶下神经。未切断神经者,双侧半月神经节有标记神经元,双侧三叉神经脊束核、三叉神经感觉主核和延髓网状结构有标记终支;切断一侧眶下神经者,只对侧有标记;无论切断眶下神经与否,双侧面神经核均有标记细胞。对其投射的功能意义也作了讨论。     

家兔臂旁内侧核在“人中”穴加压效应中的作用

Experiments were performed on 18 rabbits, bivagotomized, gallamine triethiodide immobilized and artificially ventilated. The effects of electrical stimulation of "renzhong" (Du 26) area and NPBM, separately and simultaneously, on arterial blood pressure and heart rate were observed before and after electrolytic or chemical lesion of NPBM. Electrical stimulation of "renzhong" area or NPBM, separately could elicit an increase in arterial blood pressure; when both "renzhong" and NPBM were stimulated, the pressor response to NPBM stimulation could affect that of "renzhong" area's. But after electrolytic or chemical lesion of NPBM, stimulation of NPBM could no longer elicit pressor response. On the other hand, the pressor response induced by electrical stimulation of "renzhong" area decreased obviously, after lesion of NPBM or transection of brain stem between NPBM and Nucleus trigemini principalis. These results suggest that NPBM might take part in the pressor response of "renzhong" area stimulation.     

家兔臂旁内侧核在“人中”穴呼吸效应中的作用研究

作者在电解和化学毁损家兔臂旁内侧核(NPBM)前、后,分别及同时电刺激“人中”穴,观察NPBM对膈神经放电(PND)活动的影响。结果:毁损前电刺激“人中”穴在NPBM内记录到诱发电位(EP),刺激EP记录点能引起PND的兴奋效应,并能改变单独刺激人中穴的PND效应;毁损后刺激毁损点不表现NPBM的PND效应。由此表明,在整体情况下NPBM可能参与了“人中”穴的呼吸效应。