绞股兰治疗氯丙嗪所致白细胞减少2例报告

绞股兰治疗氯丙嗪所致白细胞减少２例报告东风汽车公司茅箭医院（４４２０１２）姜学军，李新胜１病例报告例１，女，３４岁，住院号１２８７。体检内科和神经系统未见异常。诊断为精神分裂症。给予氯丙嗪治疗５００ｍｇ／日，二个月后血象由入院时的４．３×１０９／Ｌ降...     

电子文献数据库收录期刊的质量瑕疵及对策

 

以限题检索为手段，以收录期刊所载论文为指标，比较分析了国内常用的4种医学电子文献数据库收录医学期刊的质量。结果表明4种医学电子文献数据库均存在收录“非法医学期刊”之瑕疵，但各数据库的收录量有差异。提示广大读者和电子文献数据库使用者注意，并针对此问题提出了相应的对策和建议。

     

精神分裂症患者的血液流变学与正常人的对照分析

精神分裂症患者的血液流变学与正常人的对照分析东风汽车公司茅箭医院（４４２０１２）精神科姜学军检验科杨玉声精神分裂症的病因及病理机制尚不十分明确。为探索精神分裂症的血液流变学特性，通过２９例精神分裂症患者血液流变学检测结果，与我院体检的健康正常人中条件...     

内镜微波治疗食管狭窄的疗效研究

内镜微波治疗是国外 80年代开展的一项内镜治疗新技术。日本 1982年开始将内镜微波治疗用于消化道出血。我国1991年南京大学医学院附属鼓楼医院张志宏教授用于治疗消化道息肉 [1 ] 。以后又开展治疗食管癌。 1994年福建医学院附属协和医院陈红绉等报导了内镜微波治疗吻合口狭窄 [2 ]。但尚未见对食管平滑肌瘤采用微波治疗的报导。我们于 1995年 3月开始采用南京新技术研究所研制的电脑 4型微波治疗仪治疗消化道息肉。在此基础上于 1996年 1月～ 1998年 1月开展了内镜微波治疗不同原因引起的食管狭窄共 2 7例 ,行38次治疗 ,取得了良好的效果 …     

电子文献数据库收录期刊的质量瑕疵及对策

以限题检索为手段，以收录期刊所裁论文为指标，比较分析了国内常用的4种医学电子文献数据库收录医学期刊的质量。结果表明4种医学电子文献数据库均存在收录“非法医学期刊”之瑕疵，但各数据库的收录量有差异。提示广大读者和电子文献数据库使用者注意，并针对此问题提出了相应的对策和建议。     

雄激素剥夺条件下阻断自噬增加LNCaP细胞凋亡

Objective To investigate the change of apoptosis in LNCaP cells after inhibition of autophagic process under androgen removal conditions. Methods The autophagic level was deter-mined by using confocal microscopy and RT-PCR. The DAPI staining was used to indicate the apopto-sis of LNCaP cells after inhibition of autophagic by 3-MA. Also, Z-VAD-FMK was used to extend the apoptosis results. Results ①Androgen deprivation led to increased autophagy in LNCaP cells. LN-CaP cells cultured in complete medium(CM) presented low autophagic process with 1.9 scores. After 24 hours, the punetate GFP-LC3 structures were accumulated in the cells cultured in serum-free medi-um (SF)(2.64 scores). In contrast, the number of punctate GFP-LC3 remained at a very low level (1.85 scores), when cells were incubated with DHT in SFA(serum-free medium+DHT). Statistical analysis showed the significant difference between SF and SFA (P<0.01). Semiquantitative RT-PCR was employed to examine the mRNA expression of LC3. Indeed, cells grown in the medium without serum had a higher LC3 mRNA expression with the highest at 12 hour time point as compared with the cells grown in CM. DHT treatment reduced the level of LC3 mRNA. ②Blockage of autophagy by 3-MA increased the apoptosis of LNCaP cells. LNCaP cells in SF and SFA just presented a basal level of apoptosis, which is (3.19±1.09)% and (3.01±0.33)% , respectively. Under androgen-free con-ditions, inhibition of autophagy by 3-MA could increase apoptosis significantly(10. 90±2.91%). While Z-VAD-FMK, a pan Caspase inhibitor, was able to suppress this apoptotic process to the level of (1.16±0.52)%, which was statistically significant(P<0.01). Conclusions Androgen removal can lead to the increase of autophagy in LNCaP cells. Moreover, inhibition of autophagy promotes the occurrence of apoptosis.     

雄激素剥夺条件下阻断自噬增加LNCaP细胞凋亡

Objective To investigate the change of apoptosis in LNCaP cells after inhibition of autophagic process under androgen removal conditions. Methods The autophagic level was deter-mined by using confocal microscopy and RT-PCR. The DAPI staining was used to indicate the apopto-sis of LNCaP cells after inhibition of autophagic by 3-MA. Also, Z-VAD-FMK was used to extend the apoptosis results. Results ①Androgen deprivation led to increased autophagy in LNCaP cells. LN-CaP cells cultured in complete medium(CM) presented low autophagic process with 1.9 scores. After 24 hours, the punetate GFP-LC3 structures were accumulated in the cells cultured in serum-free medi-um (SF)(2.64 scores). In contrast, the number of punctate GFP-LC3 remained at a very low level (1.85 scores), when cells were incubated with DHT in SFA(serum-free medium+DHT). Statistical analysis showed the significant difference between SF and SFA (P<0.01). Semiquantitative RT-PCR was employed to examine the mRNA expression of LC3. Indeed, cells grown in the medium without serum had a higher LC3 mRNA expression with the highest at 12 hour time point as compared with the cells grown in CM. DHT treatment reduced the level of LC3 mRNA. ②Blockage of autophagy by 3-MA increased the apoptosis of LNCaP cells. LNCaP cells in SF and SFA just presented a basal level of apoptosis, which is (3.19±1.09)% and (3.01±0.33)% , respectively. Under androgen-free con-ditions, inhibition of autophagy by 3-MA could increase apoptosis significantly(10. 90±2.91%). While Z-VAD-FMK, a pan Caspase inhibitor, was able to suppress this apoptotic process to the level of (1.16±0.52)%, which was statistically significant(P<0.01). Conclusions Androgen removal can lead to the increase of autophagy in LNCaP cells. Moreover, inhibition of autophagy promotes the occurrence of apoptosis.     

雄激素剥夺条件下阻断自噬增加LNCaP细胞凋亡

目的 探讨雄激素剥夺条件下阻断自噬后LNCaP细胞凋亡变化与半胱天冬酶(caspase)激活的关系.方法 应用激光共聚焦显微镜、RT-PCR方法观察雄激素剥夺致细胞自噬增加后,利用DAPI染色观察细胞凋亡变化及药物抑制caspase后对凋亡的影响.结果 ①雄激素去除后LNCaP细胞自噬体增加,标准培养基(CM)培养下LNCaP细胞自噬体数量为1.90分;无血清培养基(SF)中细胞自噬体数量增高为2.64分;加入双氧睾酮(SFA组)后细胞自噬体下降至1.85分(P<0.01).CM中LNCaP细胞LC3 mRNA表达率为23%,血清饥饿12 h后,LC3表达量上调至100%,而SFA组LC3 mRNA表达量为86%;血清饥饿24 h后,SF组LC3 mRNA表达量为62%,SFA组为35%.②SF组和SFA组LNCaP细胞基础凋亡率分别为(3.19±1.09)0A和(3.01±0.33)%,加入3-甲基腺嘌呤(3-MA)阻断自噬24 h后,SF组凋亡率为(10.90±2.91)%,SFA组为(4.63±1.69)%.SF+3-MA组中加入Z-VAD-FMK后,细胞凋亡减至(1.16±0.52)%.组间差异有统计学意义(P<0.01).结论 剥夺雄激素后LNCaP细胞中自噬明显增加,阻断自噬后凋亡发生率增加.     

以血栓形成为首发症状的阵发性睡眠性血红蛋白尿（PNH）1例

患者 ,老年男性 ,67岁 ,因头昏 ,言语不清 ,左侧肢体活动障碍 12小时入院。既往有缺血性心脏病史 10余年 ,半月前患者左下肢有静脉血栓形成 ,曾在当地医院给予中药治疗。入院查体 :体温 3 6.4℃ ,脉搏 80次 /分 ,血压 14 / 10ｋＰａ ,呼吸 2 0次 /分 ,神志清 ,中度贫血貌 ,口唇无紫绀 ,双肺呼吸音清 ,未闻及罗音 ,心率 80次 /分 ,律齐。腹软 ,无压痛。左侧鼻唇沟略浅 ,口角右歪 ,伸舌左偏。左侧肢体肌力Ⅳ级 ,肌张力正常 ,病理征 ( - )。心电图示Ｖ3～Ｖ6 ＳＴ段下移 0 .0 5ｍＶ ,Ｔ波低平。颅脑ＣＴ示右基底节脑梗塞。髂穿骨髓常规示幼红细…     

腹腔镜联合十二指肠镜同期治疗胆囊结石合并肝外型肝胆管结石疗效观察

目的观察对胆囊结石合并肝外型肝胆管结石患者采用双镜(腹腔镜和十二指肠镜)联合治疗的临床效果。方法收集110例胆囊结石合并肝外型肝胆管结石患者作为研究对象,以患者及患属对治疗、此次研究知情同意为前提,根据临床不同治疗方式将其分为研究组(双镜同期治疗)、对照组(常规小切口开腹治疗)两个组别,病例数分别为60例、50例,观察治疗效果及并发症发生情况差异。结果同对照组相比研究组患者手术时间更短、术后下床活动时间早,且住院天数也更少(P0.05);研究组并发症发生率5.0%明显较对照组的18%更低(P0.05)。结论腹腔镜联合十二指肠镜同期治疗胆囊结石合并肝外型肝胆管结石效果好,术后下床早,恢复快,是一种新型有效肝胆部结石治疗手段。