排序方式: 共有69条查询结果,搜索用时 15 毫秒
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目的:探讨腹腔镜联合胆道镜微创保胆取石术治疗胆囊结石的安全性和临床应用价值.方法:回顾分析从2007年5月~2009年5月期间29例胆囊结石患者实施腹腔镜联合胆道镜保胆取石,术后进行随访观察.结果:术后超声检查证实29例患者胆囊结石全部被清除,无手术死亡病例.术后无出血、胆漏、急性胆囊炎、胰腺炎、继发性胆总管结石及切口感染等并发症发生.术后2周至术后1个月复查胆囊收缩功能正常,超声见胆囊大小、形态正常.随访6~12个月,无结石复发.结论:腹腔镜辅助胆道镜保胆取石术治疗胆囊结石及胆囊息肉样病变安全可靠,效果良好,术后并发症少,技术难度小. 相似文献
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Objective To investigate the change of apoptosis in LNCaP cells after inhibition of autophagic process under androgen removal conditions. Methods The autophagic level was deter-mined by using confocal microscopy and RT-PCR. The DAPI staining was used to indicate the apopto-sis of LNCaP cells after inhibition of autophagic by 3-MA. Also, Z-VAD-FMK was used to extend the apoptosis results. Results ①Androgen deprivation led to increased autophagy in LNCaP cells. LN-CaP cells cultured in complete medium(CM) presented low autophagic process with 1.9 scores. After 24 hours, the punetate GFP-LC3 structures were accumulated in the cells cultured in serum-free medi-um (SF)(2.64 scores). In contrast, the number of punctate GFP-LC3 remained at a very low level (1.85 scores), when cells were incubated with DHT in SFA(serum-free medium+DHT). Statistical analysis showed the significant difference between SF and SFA (P<0.01). Semiquantitative RT-PCR was employed to examine the mRNA expression of LC3. Indeed, cells grown in the medium without serum had a higher LC3 mRNA expression with the highest at 12 hour time point as compared with the cells grown in CM. DHT treatment reduced the level of LC3 mRNA. ②Blockage of autophagy by 3-MA increased the apoptosis of LNCaP cells. LNCaP cells in SF and SFA just presented a basal level of apoptosis, which is (3.19±1.09)% and (3.01±0.33)% , respectively. Under androgen-free con-ditions, inhibition of autophagy by 3-MA could increase apoptosis significantly(10. 90±2.91%). While Z-VAD-FMK, a pan Caspase inhibitor, was able to suppress this apoptotic process to the level of (1.16±0.52)%, which was statistically significant(P<0.01). Conclusions Androgen removal can lead to the increase of autophagy in LNCaP cells. Moreover, inhibition of autophagy promotes the occurrence of apoptosis. 相似文献
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目的:研究Cox7a2的荧光表达载体在TM3睾丸Leydig细胞中的表达和定位及对ERK1/2磷酸化的影响。方法:将Cox7a2克隆到pEYFP-N1。细胞转染和细胞荧光显微镜观察其在TM3细胞中的表达和定位,Westernblot检测ERK1/2磷酸化水平。结果:在TM3细胞中,绿色荧光YFP-Cox7a2和红色荧光线粒体标记物-Mitotracker有完全的共定位,过量表达YFP-Cox7a2能引起ERK1/2磷酸化水平增高。结论:成功表达了YFP-Cox7a2融合蛋白,确认了其在TM3细胞的线粒体定位。YFP-Cox7a2过表达和ERK1/2磷酸化水平有联系,为探讨Cox7a2对TM3细胞睾酮分泌及其相关信号传导通路的影响奠定了基础。 相似文献
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目的:分析不同内镜下止血方式治疗非静脉曲张消化道出血的疗效。方法:本次的100例研究对象均选自本院2016年1月-2017年12月接收的非静脉曲张消化道出血患者,将其依照不同治疗方式分为氩气组、钛夹组、电凝组、注射组和联合组,各20例。对比各组患者的临床治疗成效。结果:联合组的即时出血率(100.0%)、再出血率(5.0%)和72h止血率(95.0%)均明显优于氩气组、钛夹组、电凝组和注射组,差异明显,统计学有意义(X~2=4.251,4.962,4.365,4.214,P0.05)。结论:对非静脉曲张消化道出血患者实施内镜下联合治疗方式,72小时内的治学成效十分显著,值得进一步采纳推广。 相似文献
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目的观察中西医结合治疗肝硬化合并自发性细菌性腹膜炎(SBP)的临床疗效。方法72例肝硬化合并sBP患者分成治疗组(n=43)和对照组(n=29),对照组予内科常规治疗,治疗组在对照组治疗基础上采用加味小承气汤(生夫黄、枳实、厚朴、蒲公英、生地、赤芍、栀子、丹参)保留灌肠。治疗两周后观察临床效果。结果治疗组临床治愈17例,有效20例,无效6例,总有效率为86.05%。对照组29例,临床治愈8例,有效10例,无效11例,总有效率为62.07%。两组总有效率比较,差异有统计学意义(P〈0.05)。结论加味小承气汤对肝硬化合并SBP有清热解毒、轻下热结的功效,中西医结合方法治疗肝硬化合并SBP效果良好,值得进一步推广使用。 相似文献
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我院1998年4月~2003年2月共收治髌骨骨折72例。其中53例采用单纯丝线缝合固定,术后经石膏固定和CPM锻炼,获得良好结果,现报告如下。 相似文献
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