The FAST module: an add-on unit for driving commercial scanning probe microscopes at video rate and beyond

We present the design and the performance of the FAST (Fast Acquisition of SPM Timeseries) module, an add-on instrument that can drive commercial scanning probe microscopes (SPM) at and beyond video rate image frequencies. In the design of this module, we adopted and integrated several technical solutions previously proposed by different groups in order to overcome the problems encountered when driving SPMs at high scanning frequencies. The fast probe motion control and signal acquisition are implemented in a way that is totally transparent to the existing control electronics, allowing the user to switch immediately and seamlessly to the fast scanning mode when imaging in the conventional slow mode. The unit provides a completely non-invasive, fast scanning upgrade to common SPM instruments that are not specifically designed for high speed scanning. To test its performance, we used this module to drive a commercial scanning tunneling microscope (STM) system in a quasi-constant height mode to frame rates of 100 Hz and above, demonstrating extremely stable and high resolution imaging capabilities. The module is extremely versatile and its application is not limited to STM setups but can, in principle, be generalized to any scanning probe instrument.     

Frequency recovery techniques for TM/TC satellite modem in critical scenarios

Reliability and effectiveness are essential features of satellite transceivers for telemetry and telecommand applications. Modem performance has a strong impact on the success of a satellite mission, in particular, during critical scenarios as the early operation phase, the disposal of a satellite at the end of its life, or the deep‐space missions. In these specific mission critical scenarios, fast and correct data reception is even more important than high channel capacity. An unknown and fast variable channel condition, which can be caused by uncertain spacecraft attitude and large Doppler shift with respect to the data rate, requires efficient and innovative receiver architecture. This paper introduces a complete digital implementation of a transceiver for TM/TC application in low Earth orbit mission that is perfectly compliant with aforementioned requirements. Particular attention is dedicated to the definition and selection of the most appropriate frequency recovery technique; 2 open‐loop techniques that are derived from ML optimal estimator are presented and compared. Additionally, the performance of the proposed receiver is extensively studied and compared with an incoherent technique that is based on the double differential PSK modulation and is known to be suitable for sat‐com in critical scenarios.     

Effect of Asynchronous Earthquake Motion on Complex Bridges. I: Methodology and Input Motion

Based on observed damage patterns from previous earthquakes and a rich history of analytical studies, asynchronous input motion has been identified as a major source of unfavorable response for long-span structures, such as bridges. This study is aimed at quantifying the effect of geometric incoherence and wave arrival delay on complex straight and curved bridges using state-of-the-art methodologies and tools. Using fully parametrized computer codes combining expert geotechnical and earthquake structural engineering knowledge, suites of asynchronous accelerograms are produced for use in inelastic dynamic analysis of the bridge model. Two multi-degree-of-freedom analytical models are analyzed using 2,000 unique synthetic accelerograms with results showing significant response amplification due to asynchronous input motion, demonstrating the importance of considering asynchronous seismic input in complex, irregular bridge design. The paper, Part 1 of a two-paper investigation, presents the development of the input motion sets and the modeling and analysis approach employed, concluding with sample results. Detailed results and implications on seismic assessment are presented in the companion paper: Effect of Asynchronous Motion on Complex Bridges. Part II: Results and Implications on Assessment.     

A way to super resolution by a sampling method using the spectral properties of one-dimensional multilayer systems

A way to perform sampling of the evanescent spectrum of an object is considered by using a photonic bandgap (PBG). The coupling between the scattered field from the object and the PBG is discussed, showing a connection of the guide modes with selected spectral components of the scattering object in free space. Some useful examples have been discussed, showing good agreement between numerical results and theoretical previsions.     

Detailed kinetic modeling of the combustion of the four butanol isomers in premixed low-pressure flames

With regard to the importance of butanol as a potential replacement or additive to fossil transportation fuels, a detailed understanding of butanol combustion chemistry is desirable. Routes to different isomers of butanol from biomass are becoming available, and it is known that the structure of fuel molecules can be of crucial importance with respect to the intermediate species pool and the nature and amount of potential pollutants, including regulated air toxics. Quantitative major and intermediate species profiles for the combustion of the four butanol isomers under low-pressure premixed flame conditions, measured with two different in situ mass spectrometric instruments have recently been reported (P. Oßwald et al., Combust. Flame 158 (2011) 2–15), and this large consistent dataset has motivated us to further develop a detailed and comprehensive chemical kinetic model which was used here to complement the experimental results with numerical simulations, including reaction flow analyses. The major differences in the overall chemical pathways in these flames will be discussed, especially with respect to the formation of undesired emissions, and from agreement and differences between experiment and model, suggestions for further work will be given.     

Short communication: Comparison of growth kinetics at different temperatures of Streptococcus macedonicus and Streptococcus thermophilus strains of dairy origin

Within the genus Streptococcus, S. thermophilus and S. macedonicus are the 2 known species related to foods. Streptococci are widely used as starter cultures to rapidly lower milk pH. As S. macedonicus has been introduced quite recently, much less information is available on its technological potential. Because temperature is an important factor in fermented food production, we compared the growth kinetics over 24 h of 8 S. thermophilus and 7 S. macedonicus strains isolated from various dairy environments in Italy, at 4 temperatures, 30°C, 34°C, 37°C and 42°C. We used the Gompertz model to estimate the 3 main growth parameters; namely, lag phase duration (λ), maximum growth rate (µ_max), and maximum cell number at the stationary phase (N_max). Our results showed significant differences in average growth kinetics between the 2 species. Among the strains tested, 37°C appeared to be the optimal temperature for the growth of both species, particularly for S. macedonicus strains, which showed mean shorter lag phases and higher cell numbers compared with S. thermophilus. Overall, the growth curves of S. macedonicus strains were more similar to each other whereas S. thermophilus strains grew very differently. These results help to better define and compare technological characteristics of the 2 species, in view of the potential use of S. macedonicus in place of S. thermophilus in selected technological applications.     

Variations in milk protein fractions affect the efficiency of the cheese-making process

The aim of this study was to assess the influence of the amounts of the α_S1-, α_S2-, β-, and κ-casein (CN) and the α-lactalbumin and β-lactoglobulin protein fractions on the efficiency of the cheese-making process independently of their genetic polymorphisms. The study was carried out on milk samples from 1,271 Brown Swiss cows from 85 herds classified into 4 categories according to management, feeding, and housing characteristics (traditional and modern systems). To assess the efficiency of the cheese-making process, we processed the milk samples according to a laboratory cheese-making procedure (1,500 mL/sample) and obtained the following measures: (1) 3 percentage cheese yields (%CY_curd, %CY_solids, %CY_water), (2) 2 daily cheese yields obtained by multiplying %CY (curd and total solids) by daily milk yields (dCY_curd, dCY_solids), (3) 4 measures of nutrient recovery in the curd (REC_fat, REC_protein, REC_solids, REC_energy), and (4) 2 measures of cheese-making efficiency in terms of the ratio between the observed and theoretical %CY (Ef-%CY_curd, Ef-%CY_solids). All the aforementioned traits were analyzed by fitting 2 linear mixed models with protein fractions as fixed effects expressed as percentage in the milk (model M-%milk) and as percentage of the total casein content (model M-%cas) together with the effects of total casein content (only in model M-%cas), daily milk yield (only in model M-%milk; not for dCY traits), dairy system, herd (random effect), days in milk, parity, and vat. The efficiency of overall cheese yield (Ef-%CY_curd) was mostly positively associated with β-CN content in the milk, whereas Ef-%CY_solids was greater with higher amounts of κ-CN and α_S1-CN (M-%milk) due to the strong influence of both fractions on the recovery rate of milk components in the curd (fat and total solids, protein with α_S1-CN only) when expressed as percentage of milk and of total casein; only β-CN was more important for REC_protein. In contrast, we found β-lactoglobulin to be highly negatively related to all the traits related to the cheese-making process and to the daily cheese yield per cow, whereas α-lactalbumin was positively associated with the latter traits. Additional research on this topic is needed, with particular focus on the genetic and genomic aspects of the role of protein fractions in the cheese-making process and on the associations between genetic polymorphisms in milk protein and milk nutrient recovery in the curd.     

Carbonic anhydrase inhibitors: design of membrane-impermeant copper(II) complexes of DTPA-, DOTA-, and TETA-tailed sulfonamides targeting the tumor-associated transmembrane isoform IX

The synthesis and carbonic anhydrase (CA, EC 4.2.1.1) inhibitory activity of two series of aromatic sulfonamides and their Cu(II) derivatives, incorporating metal-complexing moieties of the DTPA, DOTA, and TETA type are reported. The new compounds were designed in such a way as to possess high affinity for Cu(II) ions, exploiting four pendant carboxylate moieties in the DTPA derivatives, as well as the cyclen/cyclam macrocyles, and three pendant acetate moieties in the DOTA and TETA derivatives. The new derivatives showed modest inhibition of the cytosolic isoform CA I (K(I) values in the range of 66-2130 nM), were better CA II inhibitors (K(I) values in the range of 21-360 nM), and excellent inhibitors of the tumor-associated isoform CA IX (K(I) values in the range of 4.1-110 nM), with selectivity ratios for the inhibition of the tumor (CA IX) over the cytosolic (CA II) isozyme in the range of 10.74-20.88 for the best derivatives. Copper complexes were more inhibitory than the corresponding ligand sulfonamides, and showed membrane impermeability, thus, having the possibility to specifically target the transmembrane CA IX that has an extracellular active site. Incorporation of radioactive copper isotopes in this type of CA inhibitor may lead to interesting diagnostic/therapeutic applications for such compounds.