一株新的急性髓系白血病细胞系SH-2的建立及其鉴定

Objective To establish and characterize a novel human myeloid leukemia cell line SH-2. Methods Bone marrow mononuclear cells(BMMNC) isolated from a AML-M2 patient, who failed to ob-tain complete remission after chemotherapy and allogenic bone marrow transplantation were passed in a long term IMDM culture medium supplemented with 20% fetal calf serum. Stromal cells were retained and rh-IL-3was added in the culture system. A new human myeloid leukemia cell line SH-2 was successfully established with a cytogeuetic characteristics of a loss of Y chromosome(- Y), a derivative chromosome 16 resulting from unbalanced translocation between chromosome 16 and 17, monosome 17, trisomy 19 and p53 alteration. Vari-ous methods were employed to characterize SH-2 cell line. Results SH-2 cells has been maintained without cytokine and stromal cells for more than 3 years without EB virus and mycoplasma contamination. SH-2 cells had the basically same morphological, immunophenotypic and cytogenetic features as the patient' s leukemia cells did, such as myeloid morphology, an immunophenotype of CD13+ , CD33+ , CD56+ , CD16/56+ and a hypodiploid karyotype of 45, X, - Y, der(16) t(16;17) (q24;q12) , - 17, + 19, which were gradually de-creased and replaced by the near-tetraploid cells with a karyotype of 73 - 102 (80), XX, - Y, - Y, del (lq31) ×2, der(16)t(16;17) (q24;q12) ×2, - 17, - 17, + 19, + 19. FISH and multiple FISH delineated all the abnormalities and revealed a loss of one p53 allele due to monosomy 17. DNA direct sequencing detec-ted a point mutation of CAG to CAT at codon 576 of exon 5 in another p53 allele. RT-PCR showed that SH-2 cells expressed apoptosis-related genes (bcl-2, Fas, GST- π and p21) rather than MDR-related genes. Short tandem repeat PCR provided powerful evidence for the derivation of SH-2 cell line from the patient' s leukemia cells. SH-2 cells had certain colony formation and tumorigenic capacities in nude and SCID mice. Conclu-sion SH-2 is a new myeloid leukemia cell line with a unique biology background, and will provide a useful tool for leukemia research.     

一株新的急性髓系白血病细胞系SH-2的建立及其鉴定

Objective To establish and characterize a novel human myeloid leukemia cell line SH-2. Methods Bone marrow mononuclear cells(BMMNC) isolated from a AML-M2 patient, who failed to ob-tain complete remission after chemotherapy and allogenic bone marrow transplantation were passed in a long term IMDM culture medium supplemented with 20% fetal calf serum. Stromal cells were retained and rh-IL-3was added in the culture system. A new human myeloid leukemia cell line SH-2 was successfully established with a cytogeuetic characteristics of a loss of Y chromosome(- Y), a derivative chromosome 16 resulting from unbalanced translocation between chromosome 16 and 17, monosome 17, trisomy 19 and p53 alteration. Vari-ous methods were employed to characterize SH-2 cell line. Results SH-2 cells has been maintained without cytokine and stromal cells for more than 3 years without EB virus and mycoplasma contamination. SH-2 cells had the basically same morphological, immunophenotypic and cytogenetic features as the patient' s leukemia cells did, such as myeloid morphology, an immunophenotype of CD13+ , CD33+ , CD56+ , CD16/56+ and a hypodiploid karyotype of 45, X, - Y, der(16) t(16;17) (q24;q12) , - 17, + 19, which were gradually de-creased and replaced by the near-tetraploid cells with a karyotype of 73 - 102 (80), XX, - Y, - Y, del (lq31) ×2, der(16)t(16;17) (q24;q12) ×2, - 17, - 17, + 19, + 19. FISH and multiple FISH delineated all the abnormalities and revealed a loss of one p53 allele due to monosomy 17. DNA direct sequencing detec-ted a point mutation of CAG to CAT at codon 576 of exon 5 in another p53 allele. RT-PCR showed that SH-2 cells expressed apoptosis-related genes (bcl-2, Fas, GST- π and p21) rather than MDR-related genes. Short tandem repeat PCR provided powerful evidence for the derivation of SH-2 cell line from the patient' s leukemia cells. SH-2 cells had certain colony formation and tumorigenic capacities in nude and SCID mice. Conclu-sion SH-2 is a new myeloid leukemia cell line with a unique biology background, and will provide a useful tool for leukemia research.     

肺炎克雷伯菌中检出16S rRNA甲基化酶基因mtB及β内酰胺酶基因blaLAP-2

肺炎克雷伯菌(klebsiella pneumoniae,Kpn)是医院感染病原菌中最常见菌种之一,对包括广谱β内酰胺类、氮基糖苷类和氟喹诺酮类在内的常用抗菌药物呈现多重耐药性日趋严重,甚至出现了对碳青霉烯类抗生素耐药的临床分离株[1-2],导致抗菌感染治疗相当困难.     

耐亚胺培南铜绿假单胞菌Ⅰ类整合子遗传标记及β-内酰胺类抗生素耐药相关基因研究

目的 了解临床分离的耐亚胺培南铜绿假单胞菌（IRPa）中Ⅰ类整合子及β-内酰胺类抗生素耐药相关基因存在状况。方法 自临床分离28株IRPa，采用聚合酶链反应及序列分析的方法检测Ⅰ类整合子遗传标记（intll和qacEace△I-sull基因）及18种β-内酰胺类抗生素耐药相关基因。结果 28株中，intll、qacEΔ1-sull、blaTEM、blaoxA-30群和blaCARB基因阳性株数（％）分别为21株（75．0％）、24株（85．7％）、20株（71．4％）、1株（3．6％）和1株（3．6％），经序列分析分别确认为blaaxA-J0（GenBank注册号：AY841859）和blaCARB-3,25株（89．3％）oprD基因缺失，blaIDM、blaVIM、blaSHV、blaHR、blaVEB、blaCES、blaMIR、blaDHA、blaSPM、blaGIM、blaaxA，群、blaoxA-2群、blaBEL-1、blaCTX-M-1群基因均阴性。结论 临床分离的IRPa中Ⅰ类整合子携带率很高，至少存在blaTEM、boxA-10和blaCARB-3种β-内酰胺酶基因，oprD基因缺失突变是其对亚胺培南耐药的主要原因。     

高血压对2型糖尿病患者心脏自主神经病变的影响

目的 探讨高血压对2型糖尿病患者心脏自主神经病变的影响。方法 应用短时程心率变异性分析(Heart rate variability，HRV)，对70例糖尿病患者及71例正常对照组测定了HRV，并与有关影响因素作相关分析。结果 糖尿病患者各年龄组的心率变异时域分析指标参数均较对照组降低，HRV与年龄、糖尿病病程、高血压病程呈显著负相关。结论 心脏自主神经病变为2型糖尿病常见的并发症，高血压是加重心脏自主神经病变的因素之一。     

三维试验法检测鲍曼不动杆菌产超广谱β内酰胺酶和AmpC酶

为了解我院鲍曼不动杆菌对β 内酰胺类抗生素耐药原因,我们采用三维试验法对临床分离的菌株进行超广谱β 内酰胺酶(ESBLs)和AmpC酶检测。一、材料和方法1.菌株来源及鉴定　 60株菌株均分离自我院 2000年 6月~2002年 12月住院患者临床标本,分别为痰液 33份、创伤伤口分泌物 13份、烧伤创面分泌物 10份、气管切开口分泌物 2份、中段尿和血液各 1份,采用法国生物梅里埃公司API20NE鉴定菌种。标准菌株为大肠埃希菌(ATCC25922)和铜绿假单胞菌 (ATCC27853);肺炎克雷伯菌(ATCC700603) (产SHV 18型ESBLs)和阴沟肠杆菌(029M) (去阻…     

持续静脉泵入不同剂量舒芬太尼在小儿术后镇痛中的应用

目的探讨持续静脉泵入舒芬太尼在小儿术后镇痛中的应用。方法将45例行下腹部手术的患儿术后按照舒芬太尼不同剂量随机分为3组,采用静脉持续泵入进行术后镇痛,舒芬太尼Ⅰ组剂量为0.03μg·kg-1·h-1、舒芬太尼Ⅱ组0.04μg·kg-1·h-1、舒芬太尼Ⅲ组0.05μg·kg-1·h-1,各组均加注昂丹司琼4mg。3组均于术后4h、8h、12h、24h、48h对镇痛及镇静效果进行观察,同时记录不良反应的发生情况。结果 3个剂量组在各时点的平均镇痛效果差异有统计学意义(F组别=47.02,P﹤0.01),随时间变化的镇痛效果差异也有统计学意义(F组别×时间=16.91,P﹤0.05),舒芬太尼Ⅰ组的疼痛得分高于其余两组;3组患儿在各时点上平均的镇静效果(F组别=16.76,P﹤0.05)及随时间变化的镇静效果差异有统计学意义(F组别=11.54,P﹤0.05),舒芬太尼Ⅲ组的镇静评分高于其余两组。舒芬太尼Ⅲ组出现较多例数的恶心及呕吐,但经统计学分析3组的不良反应率差异无统计学意义(P﹥0.05)。结论静脉持续泵入舒芬太尼0.04μg·kg-1·h-1镇痛对小儿是有效、安全可行的方法,可取得良好的镇痛效果和较低的不良反应率。     

农村住院分娩补助项目效果评价

目的：评价农村孕产妇住院分娩补助项目是否提高了住院分娩率和达到了预期目标。方法：数据来源于卫生部2004--2011年《中国卫生统计年鉴》和2009--2011年的《全国妇幼卫生年报》,分析住院分娩补助项目的目标实现情况,分别计算东、中、西部地区住院分娩率的变化趋势,以及重点地区住院分娩率的提高幅度和不同基线水平下的提高幅度。结果：2010年,全国31个省、自治区、直辖市全部完成住院分娩率提高的目标,西部省份增加幅度较大。全国住院分娩率低于80％的县由2008年的334个减少至2010年的151个。结论：住院分娩补助重大专项的实施大大促进了农村孕产妇住院分娩,尤其是孕产妇住院分娩率基线水平较低的地区,区域不公平的问题在一定程度上得到改善。     

一例伴有低亚二倍体异常的多发性骨髓瘤的临床和实验研究

目的 报告1例伴有低亚二倍体复杂异常的多发性骨髓瘤病例并探讨其临床和实验室特点.方法 采用骨髓细胞短期培养法制备染色体,用R显带技术进行核型分析.用13q14、p53、Rbl、lq21一系列单色探针和IgH/CCND1双色双融合探针对其进行荧光原位杂交检测.用流式细胞仪检测DNA含量.结果 R显带核型分析提示该患者5个细胞为包含35条染色体的低亚二倍体核型,3个细胞为低亚二倍体克隆的复制,另外4个细胞为正常核型.间期荧光原位杂交证实核型中存在1号、13号、14号、17号染色体单体,且显示marl来源于11号染色体并造成CCND1基因的扩增.流式细胞仪检测DNA含量显示其有低亚二倍体克隆峰,DNA指数为0.8426.结论 低亚二倍体核型在多发性骨髓瘤中发生率极低,荧光原位杂交技术是检测多发性骨髓瘤分子异常的可靠手段.     

低流行区新发现麻风患者畸残状况调查

河北省为麻风低流行区,近年来由于低流行条件下散在发生病例特点和外来人口增加给麻风防治带来新的困难。为了解麻风新发患者的畸残状况与低流行状况相关联系。我们对2000—2009年河北省累计新发病例畸残状况进行调查。1资料与方法资料来源于河北省麻风疫情报表及新发现麻风患者病例。复发患者不计算在内。麻风诊断分型参照《麻风防治手册》2002年版。畸残分级按WHO（1998）标准分为0、Ⅰ和Ⅱ级（脱眉不计算在内）。