医学院校图书馆社区服务的调查分析

利用网络调查了我国部分医学院校图书馆社区服务的形式和服务内容,分析了医学院校图书馆社区服务的现状,并提出了提高医学院校图书馆社区服务质量的几点看法。     

基于靶基因文库的高通量测序平台建立肥厚型心肌病患者致病基因突变检测方法

目的基于靶基因文库,应用下一代半导体高通量测序平台,建立快速、准确的肥厚型心肌病(hypertrophic cardiomyopathy,HCM)常见致病基因突变检测方法,有利于HCM患者的早期预防及临床分子诊断。方法选择国内外公认的与HCM致病相关的常见基因(MYH7,MYBPC3,TNNT2,TNNI3,ACTC1,PRKAG2,MYL2和MYL3),应用Primer5及Primerselect引物设计软件对靶基因编码区进行引物设计。基于下一代半导体高通量测序平台,对2019年3月至8月在大理大学第一附属医院采集的4例HCM患者外周静脉血液标本,提取基因组,构建靶基因文库,进行上机测序,测序质量评估及变异位点注释。最后对注释分析得到的与HCM致病相关的突变位点进行Sanger测序验证。结果通过建立的高通量测序方法,检出了4个与HCM致病相关的突变位点MYBPC3-c.478 C>T,MYH7-c.161 G>A,TNNI3-c.370 G>C和MYL3-c.337 A>G,并通过金标准的Sanger测序验证,结果完全一致。结论基于靶基因文库及高通量测序平台建立的HCM患者常见致病基因突变检测方法,能为临床分子诊断实验室工作者开展遗传基因突变检测提供参考.     

高校图书馆文献信息资源建设绩效审计实施路径

分析了高校图书馆文献信息资源建设和绩效评价的研究现状,结合绩效审计的流程,总结了适合高校图书馆文献信息资源建设绩效审计的具体步骤,并详细阐述了步骤中绩效审计证据采集、评价和绩效审计评价标准的确定等关键环节,以期为当前高校图书馆文献信息资源建设提供参考。     

知识经济与高校图书馆

 

全面地论述了知识经济的由来、内涵及基本特征，揭示了知识经济与图书馆之间存在着本质的、必然的联系以及知识经济对图书馆带来的严峻挑战和发展机遇。

     

医院图书馆开展信息诊疗服务

医院图书馆利用丰富的馆藏资源和便利的网络信息为病人提供信息诊疗服务，与病人共享现代医学科学资泺，可帮助病人了解病情，提高其生存和生活质量。     

Peptide synthesis on SepharoseTM beads

NHS-activated Pharmacia HiTrap Sepharose^TM was modified with 1,3-diaminopropane to give an amino-functionalized support suitable for solid-phase peptide synthesis. The amide linker p-[(R,S)-α-[1-(9H-fluoren-9-yl)-methoxyformamido]-2,4-dimethoxybenzyl]phenoxyacetic acid was incorporated and the acyl carrier protein sequence 65-74 was synthesized manually on this support by the Fmoc procedure under controlled conditions with monitoring of the coupling reactions. The performance of the support in automated multiple synthesis in open reactors, with an Abimed AMS 422 according to standard protocols, was evaluated by the synthesis of the acyl carrier protein sequence 65-74 and two other 15-mer and 18-mer peptides. The quality of the resulting crude peptides was determined by HPLC and MALDI-MS, and compared with the same sequences synthesized in parallel on the commercial peptide synthesis resin TentaGel S RAM. The modified HiTrap material was found to be particularly suited for Fmoc solid-phase peptide synthesis and should be advantageous for the utilization of immobilized peptides and peptide libraries in biological assays. © Munksgaard 1997.     

Solid-phase synthesis of peptide-4-nitroanilides

A wide variety of Glu/Asp and Gln containing peptide-4-nitroanilides and other chromogenic peptidyl-arylamides could be quickly synthesized by a Fmoc-based solid-phase synthesis strategy employing the side-chain carboxyl groups for transient anchoring to the resin. Suitable synthons for this method, Fmoc-Glu-NH-Np and Fmoc-Asp-NH-Np. were prepared using a diphenylphosphinic chloride-mediated coupling reaction. Peptides of the common structure Suc-Ala-Phe-Pro-Xaa-NH-Np (Xaa = Glu/Asp, Gln) were synthesized and were shown to be substrates for the protease subtilisin Carlsberg (E.C.3,4,21,14a) and for peptidyl-prolyl cis/trans-isomerases (PPIases E.C.5,2,1,8,). The method was extended to amino acids possessing a side chain missing an anchor for binding to the matrix. We synthesized Suc-Ala-Phe-Pro-Gln-Phe-NH-Np anchoring the dipeptide derivative Fmoc-Glu-Phe-NH-Np with the carboxyl group to Rink amide resin using standard SPPS procedures. Additionally this procedure allowed us the preparation of peptidyl-arylamides, utilizing the commercial available Fmoc-Glu-OAll as building block. A mixture of pentapeptide-4-nitroanilides with the general sequence Ala-Ala-Xaa-Pro-Gln-NH-Np was synthesized. Electrospray ionization mass spectrometry (ESI-MS) was used to evaluate the hydrolysis of the peptide mixture by the protease subtilisin Carlsberg. It could be shown that peptides with the hydrophobic amino acids Phe. Tyr, Leu and Val in the varied P3-position were most rapidly cleaved under the chosen conditions. Hydrolysis of the Gln-NH-Np bond in Ala-Ala-Pro-Pro-Gln-NH-Np has not been observed. © Munksgaard 1996.     

主动互动:提升医学院校图书馆的服务质量

从提升医学院图书馆服务质量的角度，分析了医学院图书馆的互动活动从被动转向主动的必要性，提出了开展主动互动的准则，并对主动互动的方式进行了探讨。     

知识经济时代图书馆管理体制的改革

传统图书馆管理体制存在着诸多弊端 ,难以形成知识信息的传播中心。知识经济时代 ,图书馆管理体制的改革必须融合计算机管理、信息化管理、网络化管理、知识更新管理、智力竞争管理、人才流通管理等各种模式 ,从而建立以国家为核心、省级馆为中心、市县馆为分馆的垂直管理领导体系。