Detection of bovine DNA in raw and heat-processed foodstuffs, commercial foods and specific risk materials by a novel specific polymerase chain reaction method

The identification of beef in animal foods is a major concern not only for the prevention of commercial fraud, but also to avoid safety risks deriving from the presence of prohibited bovine material that might be harmful to both human and animal health. Here we report a novel set of bovine-specific primers, CYTbos1 (forward) and CYTbos2 (reverse), which allow the specific amplification of a 115 base pair fragment of the bovine cytochrome b gene (cytb) between nt 844 (mitochondrial site 15,590) and nt 958 (mitochondrial site 15,704), no cross-reaction being observed with DNA from another 12 frequent commercial meat species. The polymerase chain reaction product obtained is cleaved specifically by endonucleases ScaI and TspE1 to achieve further confirmation evidence. The sensitivity of the proposed method was 0.025%. The CYTbos primers successfully detected bovine DNA in meat samples processed for 20 min at 133 °C/300 kPa or for 2 h at 121 °C. CYTbos primers also detected bovine DNA in heat-processed commercial meat products exhibiting a complex nature, as well as in bovine specific risk materials. The proposed polymerase chain reaction method, aimed at detecting a small and specific fragment of the bovine mitochondrial DNA, may be especially useful for the direct identification of bovine DNA in foodstuffs subjected to severe heating under overpressure conditions.     

评价动态有效位数的述评

介绍了动态有效位数的含义及溯源性,以及评价动态有效位数的两种方法－－正统波拟合法和FFT法,并说明了其各自的优缺点,此外,详细讨论了评价动态有效位置过程中应注意的几个问题：等相位密度采信问题、整周期信号采样问题、拟合收敛性问题、拟合初始值选取问题和校准软件评价问题等。同时给出了两条具有典型代表性的动态有效位数频率特性曲线的实测例证。     

水产品可追溯信息管理系统的设计

以SQLServer2005和ASP．NET为开发平台,采用RFIO技术和zigbee技术,建立起一个多层次、多用户、多权限的水产品追溯系统。该系统主要由读写器和一个面向对象的信息管理平台组成,其中读写器通过RFID技术读写标签,并采用zigbee技术与上位机的通信,实现数据的读取、查询功能。该系统以水产品供应链中重要采集点的信息为信息源,实现信息的采集、传输、管理、存储、查询等功能。     

基于无线射频识别技术的商品猪精准养殖与管理系统设计

当前我国商品猪养殖业快速发展,但养殖设施和技术手段还比较落后。本文设计商品猪的精准养殖与管理系统,利用无线射频识别技术实现猪身份的准确快速识别,利用电子称和以太网传输技术实现猪体重和摄食量的实时采集与传输,利用可编程控制技术实现环境参数的监控,并对养殖场日常信息进行数字化管理。通过试验表明,猪身份能够准确识别,摄食量和体重能够及时准确获取,环境因子参数控制范围达到了设计要求,能够满足商品猪精准养殖与管理的需要,同时可提供商品猪养殖全过程的相关信息,供溯源查询与分析。所设计的系统为研究不同品种猪或者同一品种不同阶段的猪的采食规律、最佳饲喂效果和建立营养模型提供了第一手的资料,为猪的可溯源管理打下基础,该系统亦可以推广到其他畜禽的精准养殖过程中。     

浅议自动测试系统的校准工作

简述了集成电路自动测试系统校准方案的制订、传递与溯源方式的选择以及通过MAP方案解决IC测试设备溯源问题的试用效果。     

用β源实现X,γ标准仪器的标定及量程扩展

用ＮＰＬ－２５６０剂量计测量自备的９０Ｓｒ检验源，标定其探头与灵敏度更高的电荷测量系统组成的新剂量计，使其测量下限向低端扩展。     

Accurate Calibration of Mercury Vapour Indicators for Occupational Exposure Measurements using a Dynamic Mercury Vapour Generator

Abstract

An accurate and rapid calibration procedure for mercury vapour indicators used for occupational exposure measurements is described, using a dynamic mercury vapour generator traceable to the standard bell-jar calibration apparatus. The method provides greater flexibility, accuracy, and trace ability than the usual methods for the calibration of mercury vapour indicators used for occupational exposure measurements. The calibration procedure allows a correction to be applied to the mass concentration value displayed by the mercury vapour indicator. Measurement results from the mercury vapour indicator at the occupational exposure limit of 25 µg ·m^?3, following calibration, have been estimated to have an expanded uncertainty of 8%.     

A traceability information model for CNC manufacturing

This paper proposes an information model for tracing CNC manufacturing operations. The objective of the model is to assure that traceability data is comprehensive and available for every CNC machined product, independent of the relationship between the subcontractor and the contractor. The prominent feature of the model is a link between CNC report data for a product instance, the CAD design and the CAM data. This link enables users to browse the traceability data and understand the relationships between the manufacturing process, the CAD design and the CAM data. The link is independent of the systems used to build the CAD and CAM data, and allows the users to be sure the manufactured product contains the required characteristics. Then if a product instance fails, this linkage will make possible to analyze the trace data and identify any exceptions or unusual conditions in the manufacturing process.     

Mapping the IEC 62264 models onto the Zachman framework for analysing products information traceability: a case study

In order to face new regulation directives regarding the environment and also for improving their customer relationship, enterprises have to increasingly be more able to manage their product information during the entire lifecycle. One of the objectives among others in this paper is to deal with product traceability along the product lifecycle. To meet this objective, the information system has to be designed and, further built in such a way all information regarding products is recorded. The IEC 62264 standards define generic logical models for exchanging product and process information between business and manufacturing levels of enterprise applications. Thus, it can be a base for product information traceability. However, its complexity comes from the fact it mixes conceptual and implementation details while no methodology exists that defines how to instantiate it. Product traceability is then needed to increase its abstraction level in order to concentrate on its concepts and managing its application by providing a methodology for its instantiation. In this paper, we propose to map the IEC 62264 standard models to a particular view of Zachman framework in order to make the framework concrete as a guideline for applying the standard and for providing the key players in information systems design with a methodology to use the standard for traceability purposes.