A shape analysis framework for neuromorphometry

This paper addresses in an integrated and systematic fashion the relatively overlooked but increasingly important issue of measuring and characterizing the geometrical properties of nerve cells and structures, an area often called neuromorphology. After discussing the main motivation for such an endeavour, a comprehensive mathematical framework for characterizing neural shapes, capable of expressing variations over time, is presented and used to underline the main issues in neuromorphology. Three particularly powerful and versatile families of neuromorphological approaches, including differential measures, symmetry axes/skeletons, and complexity, are presented and their respective potentials for applications in neuroscience are identified. Examples of applications of such measures are provided based on experimental investigations related to automated dendrogram extraction, mental retardation characterization, and axon growth analysis.     

Grafting kinetics of poly(methyl methacrylate) on microparticulate silica

Grafting of poly(methyl methacrylate) (PMMA) on microparticulate silica was achieved by initiating the polymerization of MMA by 4,4′-azobis(4-cyanopentanoic acid) that was covalently bound to the silica surface. The initiator seems to be destabilized upon binding it to the silica surface. The kinetics of the graft polymerization are described and are largely affected by the Trommsdorff effect, which makes it possible to graft a high amount of PMMA on silica.     

Resource allocation in grid computing

Grid computing, in which a network of computers is integrated to create a very fast virtual computer, is becoming ever more prevalent. Examples include the TeraGrid and Planet-lab.org, as well as applications on the existing Internet that take advantage of unused computing and storage capacity of idle desktop machines, such as Kazaa, SETI@home, Climateprediction.net, and Einstein@home. Grid computing permits a network of computers to act as a very fast virtual computer. With many alternative computers available, each with varying extra capacity, and each of which may connect or disconnect from the grid at any time, it may make sense to send the same task to more than one computer. The application can then use the output of whichever computer finishes the task first. Thus, the important issue of the dynamic assignment of tasks to individual computers is complicated in grid computing by the option of assigning multiple copies of the same task to different computers. We show that under fairly mild and often reasonable conditions, maximizing task replication stochastically maximizes the number of task completions by any time. That is, it is better to do the same task on as many computers as possible, rather than assigning different tasks to individual computers. We show maximal task replication is optimal when tasks have identical size and processing times have a NWU (New Worse than Used; defined later) distribution. Computers may be heterogeneous and their speeds may vary randomly, as is the case in grid computing environments. We also show that maximal task replication, along with a c μ rule, stochastically maximizes the successful task completion process when task processing times are exponential and depend on both the task and computer, and tasks have different probabilities of completing successfully.     

Cost and Waste Comparison of Reusable and Disposable Shipping Containers for Cut Flowers

Logistics is a fundamental component of the post‐harvest processes, especially in the floricultural sector where the products have to be marketed in short times after the cutting because of their fragility. In the previous years, new approaches to efficiently manage the logistical function and to reduce costs and environmental impacts have been consolidated. In this paper, the investments for two different containers for flower transportation, one disposable and one reusable, are compared, considering a specific supply chain located in central Italy. All data were provided by stakeholders. These were divided into four groups: purchasing, administration, logistical and disposal costs. For the reusable containers, a reverse logistical system, to collect the return components, was hypothesised by using the network analysis tool of the geographic information system (GIS). Results show that the use of a reusable system, which assures a production with less waste and less environmental pressures, is also economically advantageous. Reusable containers involve an initial investment but became cheaper from the second year of the investment and also remained convenient, considering all the costs related to the return collection of the items. Reusable system permits a financial advantage of approximately 140 k€ in total and 21 k€ for each year, considering all the nine years of analysis. Logistics is responsible of the great contribution of total cost in both the reusable and the disposable container (88% and 76%), probably because of the high fragmentation in the delivery service to sale points. Reverse logistics represents the 17% of the reusable container total costs. Copyright © 2011 John Wiley & Sons, Ltd.     

Linear variable optical filter-based ultraviolet microspectrometer

An IC-compatible linear variable optical filter (LVOF) for application in the UV spectral range between 310 and 400 nm has been fabricated using resist reflow and an optimized dry-etching. The LVOF is mounted on the top of a commercially available CMOS camera to result in a UV microspectrometer. A special calibration technique has been employed that is based on an initial spectral measurement on a xenon lamp. The image recorded on the camera during calibration is used in a signal processing algorithm to reconstruct the spectrum of the mercury lamp and the calibration data is subsequently used in UV spectral measurements. Experiments on a fabricated LVOF-based microspectrometer with this calibration approach implemented reveal a spectral resolution of 0.5 nm.     

Microwave-assisted hydrothermal synthesis of zinc oxide particles starting from chloride precursor

Zinc oxide (ZnO) was synthesized using a microwave assisted hydrothermal (MAH) process based on chloride/urea/water solution and under 800 W irradiation for 5 min. In the bath, Zn²⁺ ions reacted with the complex carbonate and hydroxide ions to form zinc carbonate hydroxide hydrate (Zn₄CO₃(OH)₆·H₂O), and the conversion from Zn₄CO₃(OH)₆·H₂O to ZnO was synchronously achieved by a MAH process. The as-prepared ZnO has a sponge-like morphology. However, the initial sponge-like morphology of ZnO could change to a net-like structure after thermal treatment, and compact nano-scale ZnO particles were finally obtained when the period of thermal treatment increased to 30 min. Pure ZnO nanoparticles was obtained from calcination of loose sponge-like ZnO particles at 500 °C. The analysis of optical properties of these ZnO nanoparticles showed that the intensity of 393 nm emission increased with the calcination temperature because the defects were reduced and the crystallinity was improved.     

Microcystin-LR toxicity on dominant copepods Eurytemora affinis and Pseudodiaptomus forbesi of the upper San Francisco Estuary

This study investigates the toxicity and post-exposure effects of dissolved microcystin (MC-LR) on the dominant copepods of the upper San Francisco Estuary (SFE), where blooms of the toxic cyanobacteria Microcystis aeruginosa coincide with record low levels in the abundance of pelagic organisms including phytoplankton, zooplankton, and fish. The potential negative impact of Microcystis on the copepods Eurytemora affinis and Pseudodiaptomus forbesi has raised concern for further depletion of high quality fish food. Response of copepods to MC-LR (MC) was determined using a 48-h standard static renewal method for acute toxicity testing. Following exposure, a life table test was performed to quantify any post-exposure impacts on survival and reproduction. The 48-h LC-50 and LC-10 values for MC were 1.55 and 0.14 mg/L for E. affinis; and 0.52 and 0.21 mg/L for P. forbesi. Copepod populations recovered once dissolved MC was removed and cultures returned to optimal conditions, suggesting no post-exposure effects of MC on copepod populations. Dissolved microcystin above 0.14 mg/L proved likely to have chronic effects on the survival of copepods in the SFE. Since such high concentrations are unlikely, toxicity from dissolved microcystin is not a direct threat to zooplankton of the SFE, and other mechanisms such as dietary exposure to Microcystis constitute a more severe risk.     

2D BASIN MODELLING IN THE EASTERN VARISCAN FOLD BELT (CZECH REPUBLIC): INFLUENCE OF THRUSTING ON PATTERNS OF THERMAL MATURATION

This study evaluates the geothermal history of Palaeozoic sedimentary rocks from the easternmost part of the Variscan external zone in the NE Czech Republic. The objective was to investigate the geothermal history of pre‐Variscan Palaeozoic carbonates in the study area, and to assess its relationship with that of the overlying Variscan flysch. In the study area, the Palaeozoic succession occurs at the surface or is overlain by Miocene sediments of the Carpathian Foredeep. Palaeozoic nappes and the main Variscan overthrust have been documented in the subsurface at the deep Pot?tát‐1borehole. Vitrinite reflectance measurements on 38 samples from the Pot?tát‐1 well and 19 samples from nearby surface outcrops and shallow boreholes were available. A 2D thermal model was created using PetroMod and the thermal maturity evolution was modelled by EASY%Ro. The thermal model was constructed based on interpretations of two NW‐SE seismic profiles (lines 5/83 and 5/84) oriented perpendicular to the main Variscan thrusts. The results were calibrated using measured vitrinite reflectance and were adjusted with 1D models from three shallow boreholes. At the Pot?tát‐1 borehole, modelled maximum palaeo‐temperatures of the Variscan flysch (Moravice Formation) ranged from 310°C at a depth of 7.3 km (the top of the preserved succession) to 395°C at the base of the succession, resulting in thermal maturities of >4%R_r. Peak maturation occurred prior to the end of Variscan thrusting. Modelling suggests that the basal heat flow for these thrust units reached a maximum value of 63 mW/m² at 325 Ma. In addition, the modelling suggests that the maturity of the Palaeozoic carbonates was controlled by the thickness of the overlying Variscan flysch nappes. Maximum palaeo‐temperatures for the Palaeozoic carbonates ranged from 265°C at the top of the interval (at a depth of 7.1 km) to 290°C at the base, resulting in a maturity of 3.8 to >4%R_r which is within the dry gas window. The study suggests that basal heat flows in the original (pre‐thrust) Early Carboniferous sedimentary basin were slightly higher than those in the post‐thrust location for the Variscan flysch nappes. This should be taken into account when evaluating the petroleum system in the South Moravian oil province (SW Czech Republic) where a complete sedimentary sequence has not been preserved.     

嵌入型量测设备及封闭式控制回路在提升研磨制程能力的应用

诺威量测为第一家首先提出嵌入式量测解决方案的公司,凭借其在嵌入式量测及先进制程控制所取得的经验,提供在化学机械研磨制程上降低生产成本且可提高产品质量的测量系统。探讨嵌入型量测的观念及原理,封闭式回路控制在芯片生产上已被应用多年。     

The Role of Fc Receptors on the Effectiveness of Therapeutic Monoclonal Antibodies

Since the approval of the first monoclonal antibody (mAb) in 1986, a huge effort has been made to guarantee safety and efficacy of therapeutic mAbs. As of July 2021, 118 mAbs are approved for the European market for a broad range of clinical indications. In order to ensure clinical efficacy and safety aspects, (pre-)clinical experimental approaches evaluate the respective modes of action (MoA). In addition to antigen-specificity including binding affinity and -avidity, MoA comprise Fc-mediated effector functions such as antibody dependent cellular cytotoxicity (ADCC) and the closely related antibody dependent cellular phagocytosis (ADCP). For this reason, a variety of cell-based assays have been established investigating effector functions of therapeutic mAbs with different effector/target-cell combinations and several readouts including Fcγ receptor (FcγR)-mediated lysis, fluorescence, or luminescence. Optimized FcγR-mediated effector functions regarding clinical safety and efficacy are addressed with modification strategies such as point mutations, altered glycosylation patterns, combination of different Fc subclasses (cross isotypes), and Fc-truncation of the mAb. These strategies opened the field for a next generation of therapeutic mAbs. In conclusion, it is of major importance to consider FcγR-mediated effector functions for the efficacy of therapeutic mAbs.