移植肾组织TGF-β1和MMP-2表达及IV型胶原沉积与慢性移植肾肾病的关系

目的 探讨移植肾组织中转化生长因子β1(TGF-β1)和基质金属蛋白酶2(MMP-2)的表达及IV型胶原沉积与慢性移植肾肾病(CAN)的关系.方法 对18例CAN患者进行了移植肾切除术.光镜下观察切除的肾组织标本并根据其纤维化的程度进行分期,用免疫组织化学技术和图像分析法检测移植肾组织中TGF-β1和MMP-2表达量及IV型胶原沉积情况,并分析患者血肌酐(Cr)和尿素氮(BUN)的水平与移植肾纤维化程度的关系.结果 随着患者移植肾纤维化程度的加重,血清肌酐、尿素氮及肾组织中TGF-β1、IV型胶原基因表达量均增加,MMP-2表达量于移植肾纤维化早期明显升高,并随移植肾纤维化程度加重而逐渐降低.结论 移植肾组织中TGF-β1表达量增高及IV型胶原沉积可以促进CAN的进程,而MMP-2则可抑制其进展.     

Ⅳ型胶原和透明质酸测定早期肝纤维化的临床价值

肝纤维化是一切恶性肝病的共同病理学基础,是肝硬变的初级阶段,并与肝癌的发生有一定关系,肝纤维化是细胞外基质,合成和降解失衡的结果,在进入肝硬变之前尚有逆转的可能。Ⅳ型胶原和透明质酸是细胞外基质的主要成分,作为检测肝纤维化的指标[1]。我科通过检测56例健康人和108例肝病患者血清HA和IV-C的含量,并探讨其临床价值。     

医用胶原膜在义齿种植术后骨融合期引导骨再生作用的临床分析

目的：探讨种植术中使用医用胶原膜对术后骨融合期引导骨再生的影响。方法：对31例种植术中发现骨量不足的骨缺损区和即刻种植术中种植体与拔牙创间骨间隙较大区域采用医用胶原膜覆盖并严密缝合，术后1周，1个月，2个月，4个月连接种植体上部结构时摄X线片，对新骨生长情况进行连续性临床和X线的观察分析。结果：31例患植入种植体47枚，其中39枚种植体植入后使用了胶原膜，二期手术时种植体均已与骨组织形成理想的骨融合，顺利完成种植义齿修复。39枚种植体中有15枚种植体于术后2个月的X线片可见到种植体封闭螺帽上方骨密度增高影，4个月后二期手术切开牙龈时可见到新骨覆盖种植体表面，用骨凿等去除新骨后方可见到封闭螺帽。结论：医用胶原膜在种植术中有较好的引导骨再生利用，有利于种植术后骨融合期新骨的形成。     

大鼠脂肪干细胞复合胶原-壳聚糖-硫酸软骨素三维支架构建组织工程软骨

Objective To evaluate the character of the collagen-chitosan-chondroitin sulfate scaffold seeded with rat adipose tissue-derived stromal cells. Methods A dipose tissue were harvested from 6 weeks old Wistar rats and the stromal cells were harvested by type Ⅰ collagenase and then cultured in vitro. Type Ⅰ collagen was fully mixed with chitosan, freeze-dried and cross-linked with chondroitin sulfate, then freeze-dried again and sterilized by ethylene oxide. The pore diameter, water content, porosity of the scaffold were tested. The adipose tissue-derived stromal cells were digested, seeded into the plates, scaffold, and cen-trifuged into pellet, and then induced into cartilage. MTT detection for cell proliferation was done. After 3 weeks, the cell morphology, and cell proliferation and adhesion were observed, and chondrngenic differenti-ation was also analyzed. Results The pore diameter, water content, porosity tested for the scaffold showed an appropriate form. Cell proliferation showed faster in the scaffold and pellet culture system after 5 day, there was still cell proliferation in the scaffold system after 14 days but no obvious changes in the pellet cul-ture system; ceils on the scaffold proliferated densely showed by histological staining, but there was a scaf-fold structure residues in the inner layer. The finding of type Ⅱ immunohistochemistry stain showed that cells express strong positive for type Ⅱ collagen in the scaffold and pellet culture system whereas it was weakly positive in the plate culture system; the specific mRNA for cartilage, type Ⅱ collagen, aggrecan and SOX-9 were expressed in all three systems showed by RT-PCR, but type X collagen was expressed continu-ously in the plate culture system and expressed after 21 days in the pellet culture system, whereas it was not detected in the collagen-chitosan-chondroitin sulfate scaffold system. Conclusion The parameters of the collagen-chitosan-chondroitin sulfate scaffold were suitable in our study. The results suggested that it can promote the adipose tissue-derived stromal cells proliferation and chondrogenic differentiation better than the plate and pellet culture systems and maintain the phenotype of chondrocytes well; it is the optimal choice for cartilage tissue engineering in the future.     

粉防己碱和氯丙嗪对人胚肺成纤维细胞胶原和透明质酸合成的影响

研究粉防己碱和氯丙嗪胚肺成纤维细胞胶原与透明质酸合成的影响，为应用钙拮抗剂治器官纤维化提供依据。采用［＾３Ｈ］脯氨酸掺入和放射免疫法分别测定胶原与ＨＡ合成。结果：Ｔｅｔ５－８０μｍｏｌＬ＾－１和Ｃｈｌ１０－４０μｍｏｌＬ＾－１均以浓度依赖方式抑制胶原与ＨＡ合成。Ｔｅｔ５－２０μｍｏｌＬ＾－１对细胞无明显毒性却显著抑制胶厚与ＨＡ合成。     

慢性肾脏病患者肾组织蛋白酶B的表达及意义

目的 探讨慢性肾脏病患者肾组织蛋白酶B(cathepsin B，CB)的表达及其与肾组织纤维化的关系。方法 采用免疫组织化学SP法和计算机图像分析系统检测肾组织CB、IV型胶原的表达情况，并以体视学多能目镜测试系统对肾活检组织病理切 片进行形态计量，计算硬化肾小球比率及病变肾小管肾间质比率。结果 CB在近曲小管表达最高，在肾小球和肾小管其他部位表达较少。轻度纤维化组和中度纤维化组CB的表达明显低于无纤维化组。CB表达与Ⅳ型胶原表达、病变肾小管肾间质比率及硬化肾小球比率呈显著负相关。结论 CB主要在肾近曲小管表达，其表达成少是细胞外基质(extracellular matrtx，ECM)降解减少的原因之一，参与肾组织纤维化的发生机制。     

抗Ⅲ型前胶原肽单克隆抗体的制备及初步应用

制备出两株分泌抗ＰⅢＰ（Ⅲ型前胶原肽）单克隆抗体的杂交瘤细胞株。竞争抑制ＥＬＩＳＡ试验表明：两株单抗与ＰＣＩ（Ⅰ型前胶原）及ＣⅢ（Ⅲ型胶原）无交叉反应。Ｄｏｔ－ＥＬＩＳＡ示两株单抗钧针对ＰⅢＰ构象决定簇。应用所制备的单抗对人肝细胞癌组织及大鼠、小鼠成纤维母细胞进行免疫定位。结果显示两种成纤维母细胞呈明显膜阳性；肝癌细胞浆内呈粗颗粒状染色，提示肝癌细胞具有合成Ⅲ型前胶原的能力。     

胶原植入与美容

胶原来源及成份本品从小牛皮肤提炼制成，是一种半透明的物质，含Ⅰ型胶原95～98％，其余是Ⅱ型胶原，1ml制剂中含胶原35mg，另有磷酸盐缓冲液和利多卡因。冷藏保存。由四川省中药研究所提供。临床资料一、观察对象：本组病例21人，女11人，男10人，年龄最大48岁，最小7岁，平均21岁。痤疮后遗疤痕14例，老年性皱纹2例，外伤后遗疤痕3例，脓疱疮，疖后遗疤痕2例。其中20例未作过任何治疗，一例因外伤后遗疤痕于1年前作过磨削术，疗效不满意。     

糖尿病肾脏胶原非酶糖化与氨基胍阿斯匹林的治疗作用

本实验系统观察了ＳＴＺ所致糖尿病鼠在１、３、６个月后肾脏组织胶原发生非酶糖化的情况，及氨基胍、阿斯匹林对肾脏胶原非酶糖化的阻断作用。结果表明：各病程糖尿病动物肾组织的胶原含量和非酶糖化早期产物（５－ＨＭＦ）及胶原相联荧光值均明显高于正常对照。氨基胍能够减弱糖尿病动物肾组织胶原含量的增加和荧光产物的生成，但对５－ＨＭＦ含量无影响。阿斯匹林未显示出对胶原非酶糖化的影响。     

慢性肝病患者血清TGFβ1,I—C,Ⅲ—C水平及其与肝纤维化的关系

应用酶免疫技术对１１８例慢性肝炎，肝硬化患者进行血清转化生长因子β１（ＴＧＦβ１），Ⅰ，Ⅲ型胶原（Ⅰ－Ｃ，Ⅲ－Ｃ）含量测定，分析三者与肝功能指标间相关性，结果表明，患者血清ＴＧＦβ１，Ⅰ，Ⅲ型胶原含量均高于正常对照组，且随慢迁肝→慢活肝→肝硬化逐渐递增，组间差异显著，血清ＴＧＦβ１，与Ｉ－Ｃ，Ⅲ－Ｃ谷丙转氨酶呈正相关，与白／球比值，血清总胆红素不相关，提示ＴＧＦβ１可能是一种很有前途的肝纤维化血清