电泳四氟乙烯耐磨涂层

论述了电泳四氟乙烯的泳前处理、工艺规范、质量控制、验收技术标准。     

毛细管电泳法测定银杏叶片中的橙皮素、芦丁和槲皮素

建立了一种利用毛细管电泳测定银杏叶片中橙皮素、芦丁和槲皮素的方法。研究了缓冲溶液pH值和浓度、分离电压和进样时间对分离的影响，在优化的条件下，10min内实现了三种物质的良好分离。橙皮素、芦丁和槲皮素分别在0．03～0．80、0．06～0．00，0．04～0．90mg/mL浓度范围内和峰电流成线性关系，线性相关系数分别为0．9992、0．9976、0．9991，检出限分别为0．005、0．009和0．006mg/mL。     

毛细管SDS无胶筛分电泳测定小分子多肽相对分子质量

目的建立快速毛细管SDS无胶筛分电泳法(SDS-NGS),测定小分子多肽相对分子质量。方法涂层毛细管(管长30cm,内径100μm);分离电压为9kV(300V/cm),分离温度为20℃;检测波长为214nm,检测时间为18min;所用SDS-多肽相对分子质量标准范围为2 512~16 949;以橙G(OG)为内标参照物。结果在相对分子质量2512~16949范围内,多肽相对分子质量的对数与其相对迁移率具有良好的线性关系(r=0.996);应用该方法测定了重组人奈西立肽、重组人表皮生长因子、虎纹镇痛肽、重组人心钠肽4种重组小分子多肽的相对分子质量,所测结果变异系数CV均小于3%。结论毛细管SDS无胶筛分电泳方便快速,灵敏度高,重现性好,结果准确,可作为小分子多肽相对分子质量的检测方法。     

基于ARM和DSP的嵌入式ICE芯片扫描分析系统

集成毛细管电泳（ICE）芯片信号采集与处理系统的微型化、智能化是ICE芯片能否得到广泛应用的关键。针对传统的ICE芯片扫描分析系统结构复杂、处理速度慢、体积大等弱点,设计了一种基于DSP＋双口RAM＋ARM的嵌入式ICE芯片扫描分析系统。该系统将高速DSP与在通讯、网络和实时控制方面具有独特优势的ARM处理器,以及实时性好、接口电路简单、数据传输量大的双口RAM结合起来,为开发高性能、集成化、便携式的新型微小生化分离分析系统奠定了一定的技术基础。     

Electrochemical deposition of polypyrrole–carbon nanotube films using steroid dispersants

Polypyrrole (PPR)–carbon nanotube (CNT) films were prepared by an electrodeposition method, combining PPR electropolymerization and anaphoresis of CNT. PPR polymerization experiments showed advantages of a dopant from the catechol family for the deposition of PPR films at reduced electrode potentials. The method allowed the formation of adherent films on stainless steel substrates. The amphiphilic molecules with steroid-like structures, such as carbenoxolone disodium salt, glycyrrhizic acid, ammonium salt, and sodium taurodeoxycholate, were used for dispersion and charging of CNT. The new dispersing agents showed outstanding dispersion ability. In addition to dispersing properties, electrodeposition experiments revealed film-forming properties of carbenoxolone and ability to form pure carbenoxolone or carbenoxolone–CNT films. The PPR–CNT films formed using carbenoxolone disodium salt, glycyrrhizic acid ammonium salt, and sodium taurodeoxycholate showed diverse microstructural features. The dispersion and deposition mechanisms were discussed.     

The murine plasma protein response to polymicrobial intra-abdominal sepsis

Protein biomarkers in the peripheral blood could potentially be used as early indicators of sepsis and a means to stratify patients for clinical trials. Although individual molecular markers have been proposed for sepsis, none has clinical utility. The global changes in plasma proteins over the clinical course of sepsis have not been characterized using proteomic methods. We used cecal ligation and puncture to induce polymicrobial sepsis in mice and generated plasma protein profiles using 2‐D DIGE of plasma from septic mice and surgical controls. Replicate cohorts (n = 3) of 4–7 animals each were used to identify 62 gel features that changed significantly (Student's t‐test, p<0.05). We identified a suite of plasma proteins that describe uniquely the host plasma response to polymicrobial septic insult. Principal components analysis of protein abundance showed that ～90% of the variability between samples was due to sepsis. In addition to canonical acute phase proteins, we identified proteins that are associated with metabolic changes (e.g. α‐2 HS glycoprotein and zinc α‐2 glycoprotein) consistent with the pathophysiology of sepsis. The panel of sepsis‐associated molecular markers identified herein may prove useful in the diagnosis and categorization of sepsis.     

不同品系北京鸭血清前白蛋白多态性的研究

应用聚丙烯酰胺凝胶电泳法研究了北京鸭的血清前白蛋多态性,在2个位点观察到多态,Pam位点为单态,在Pas位点发现有5种表现型,它们的频率是：Pas2-2O 26．67％,Pas1-2为12％,Pas2-1为45.33%,Pas1-1为2．67％,Pas2-0为13．33％,在Paf位点发现有4种表现型,它们的频率是：PafF为29．33％,PafS为41．33％,PafFS为10．67％,Paf0为18．67％,并计算了它们的基因频率,群体平均杂合度和遗传距离,遗传分析表明,樱桃谷鸭和奥白星鸭遗传结构相似,而与B系北京鸭遗传结构有明显差异。     

浅谈阴极电泳涂装中涂膜形成针孔的原因及解决措施

分析了阴极电泳涂装中形成针孔的原因,提出了在实际施工过程中解决此类问题的方法以及各项工艺参数的控制。     

扑尔敏对映体的毛细管电泳分离研究

本文采用毛细管电泳对扑尔敏对映体的分离进行了讨论。测定了不同pH值条下两对映体与β-环糊精相互作用的结合常数,讨论了结合常数与分离选择性的关系。针对理论计算出的两对映体淌度差达到最大时所需的β-环糊精最佳浓度理论值与达到最大分离度所需的β-环糊精实际浓度值之间存在一定偏差的现象,结合分离度的定义,推导了分离度与对映体淌度之间的关系并进行了验证。