碳纳米管的生物相容性

碳纳米管（CNT）是一种非常有序、高纵横比的碳同素异形体，包括单壁碳纳米管（SWCNT）和多壁碳纳米管（MWCNT）。它的特性使其在生物医学领域得到广泛应用，包括生物传感器、药物和疫苗传递，以及特殊生物材料的制备。本文总结了现有碳生物材料性能，概述了纳米毒理学研究内容，探讨了CNT细胞毒性和生物相容性。     

社会支持、自我效能和应对方式对COVID-19疫情下乳腺癌患者心理弹性的影响

目的 研究社会支持、自我效能和应对方式对新型冠状病毒肺炎（COVID-19）疫情下乳腺癌患者心理弹性和作用路径的影响。方法选取确诊的799例乳腺癌患者作为研究对象。运用一般资料问卷、心理弹性量表（CD-RISC）、社会支持量表（SSQ）、自我效能量表（GSES）和应对方式量表（MCMQ）评估患者的心理弹性、社会支持、自我效能和应对方式水平,并研究三者对乳腺癌患者心理弹性水平的影响及作用路径。结果乳腺癌患者心理弹性总分为（59.72±13.3）分,社会支持总分为（32.45±8.3）分,自我效能总分为（46.7±8.2）分,对应方式总分为（48.56±10.4）分。乳腺癌患者的主观支持与自我效能对其心理弹性有直接影响和间接正效应影响,面对和屈服应对方式对心理弹性分别有直接的正效应和负效应影响,可解释72.3%的弹性心理（F=114.703,P=0.004）。结论COVID-19疫情下,乳腺癌患者心理弹性水平中等偏低。乳腺癌患者的主观支持、自我效能、面对和屈服的应对方式影响心理弹性水平。     

基于板蓝根中（R,S）-告伊春含量研究板蓝根加工方法

目的 研究不同干燥方法及软化切制方法对板蓝根中（R,S）-告伊春含量的影响,从而建立提升黑龙江产板蓝根质量的加工方法。方法 采用高效液相色谱法对板蓝根中（R,S）-告伊春含量进行测定,比较晒干与烘干板蓝根药材的（R,S）-告伊春含量差异,比较不同投料形式板蓝根颗粒和饮片中（R,S）-告伊春含量差异。结果 60 ℃烘干后的板蓝根药材中（R,S）-告伊春含量高于晒干的板蓝根药材,且能明显缩短板蓝根药材的产地干燥时间;经软化切制的板蓝根饮片中（R,S）-告伊春含量明显高于板蓝根粗颗粒。结论 在板蓝根产地加工过程中,可用烘干法代替晒干法;以板蓝根药材作为中成药投料,建议采用软化切制后的板蓝根饮片。     

胰岛素抵抗和单硝酸异山梨酯对S HR心肌细胞凋亡的影响

目的：观察胰岛素抵抗（IR）和单硝酸异山梨酯（ISMN）对自发性高血压大鼠（SHR）心肌细胞凋亡的影响。方法14周龄雄性Wistar大鼠（W）和SHR（S）各40只,分别或联合喂饲普通饲料（普）、高脂高糖（HFHG）饲料及生理盐水（NS）和ISMN灌胃。将其分为普＋NS组（W 普和S普）,HFHG ＋NS组（W高和S高）,普＋ISMN组（W单和S单）,HFHG＋ ISMN组（W高单和S高单）,每组各10只。喂养12周,取颈动脉血检测血糖及血清胰岛素水平并计算IR指数（HOM A‐IR）;取心肌组织4份,分别观察光镜下形态、组织中一氧化氮（NO）水平,bcl‐2、bax基因及其蛋白的表达情况。结果心肌NO水平、bax基因mRNA及相关蛋白表达水平,在给予 HFHG和ISMN干预的各组均高于普通组,bcl‐2基因mRNA及相关蛋白表达反之;S组大鼠心肌组织NO水平、bax基因 mRNA及相关蛋白表达均较相应的W组增多,bcl‐2基因mRNA及相关蛋白表达反之;W高组大鼠心肌组织NO水平与HOMA‐IR呈显著正相关,W单组大鼠HOMA‐IR与心肌组织NO水平与呈正相关。结论 SHR心肌细胞凋亡较Wistar大鼠增多,ISMN、IR均能加重SHR心肌细胞凋亡,且ISMN与IR互相诱导、互为因果。     

原发性肝癌患者肝动脉化疗栓塞术后心理弹性情况及影响因素分析

目的：分析原发性肝癌（PLC）患者肝动脉化疗栓塞（TACE）术后心理弹性情况及其影响因素。方法：选取2020年4月至2022年7月在该院行TACE治疗的117例PLC患者进行横断面研究。采用心理弹性量表（CD-RISC）评估患者心理弹性水平,对TACE术后PLC患者心理弹性的相关因素进行单因素和多元线性回归分析。结果：117例PLC患者TACE术后CD-RISC总分为（56.27±4.48）分,低于国内常模。不同受教育程度、TACE治疗次数、应对方式评分、家庭功能、不良反应分级、恐惧疾病进展简化量表（FOP-Q-SF）评分的PLC患者TACE术后CD-RISC评分比较,差异有统计学意义（P<0.05）。受教育程度、TACE治疗次数、应对方式评分、家庭功能、不良反应分级、FOP-Q-SF评分均是PLC患者TACE术后心理弹性的影响因素（P<0.05）。结论：受教育程度、TACE治疗次数、应对方式、家庭功能、不良反应分级、恐惧情绪等均为TACE术后PLC患者心理弹性水平的影响因素,应予以针对性预防措施,以提高其心理弹性,改善其预后。     

表现基因分型:一个预测伯-韦综合征患者肿瘤患病风险及其类型的工具

患有韦-伯综合征(BW S)的患者有7.5%～10%在儿童期发生肿瘤,其中60%的患儿发生W ilm s瘤。约70%BW S病例染色体11p15上的两个功能不同的印迹基因簇发生异常甲基化。作者的目的是研究这两个印迹基因簇(BW SIC1/2)的印迹状态和肿瘤发生率及类型之间的关系。在114例临床诊断为BW S的患者身上,收集到H19和K CNQ1O T1的甲基化模式。随访这些患者到5岁,然后记录肿瘤的发生率和类型。发现,与其他BW S患者相比,BW SIC2甲基化缺陷的患者发生儿童期肿瘤的概率较小。这些患者中没有发现W ilm s瘤,然而在BW SIC1甲基化缺陷的患者中,W ilm…     

血液滤过时不同滤器对血小板数量的影响

目的：评估血液滤过时分别应用三醋酸纤维素膜（FB190U）、聚砜膜（AV600S）两种不同的滤器对患者血小板数量的影响。方法：选取急性肾功能衰竭需要行血液滤过治疗患者21例,采用自身对照方法分别应用FB190U及AV600S治疗12 h,分别于治疗前、后检测血小板数量。结果：应用FB190U治疗前、后血小板数比较差异无统计学意义（P〉0.05）,应用AV600S治疗12 h后血小板数较治疗前明显降低,差异有统计学意义（P〈0.01）。结论：血液滤过时应用三醋酸纤维素膜（FB190U）对血小板数量的影响比应用聚砜膜（AV600S）小。     

不同浓度的多壁碳纳米管对HEK293细胞的影响

目的 研究多壁碳纳米管(MWCNT)对正常人胚肾293细胞的增生及细胞活性的影响.方法 采用离体培养的正常人胚肾293细胞株,接种人96孔板,细胞生长18h后加入不同浓度的单壁碳纳米溶液,碳纳米溶液作用于人胚肾293细胞48个h后,使用MTT(methyl thiazolyl tetrazoliun)比色法测每个孔的吸光值,计算人胚肾293细胞的生长抑制率并绘制细胞活性曲线.结果 不同浓度的多壁碳纳米管对人胚肾293细胞具有不同程度的抑制作用,且当其浓度达到0.5μg/ml时对细胞出现显著的抑制作用.结论 多壁碳纳米管能通过正常人胚肾293细胞膜进入细胞内,一定程度地影响细胞的活性,到达一定剂量时,细胞增生能力显著降低.因此,可以推测多壁碳纳米管对正常细胞生长作用有一定的安全剂量范围.     

弹性成像联合精细血流显像诊断乳腺癌腋窝淋巴结转移的价值

目的 探讨灰阶超声联合精细血流显像（fine flow）和弹性成像在评估乳腺癌腋窝淋巴结转移中的诊断价值。方法 选取乳腺癌患者的148枚同侧腋窝淋巴结,手术前分别用灰阶超声观察图像特征、测量皮质厚度及长短径,并计算纵横比（L/S）;精细血流显像观察其血供分布类型;弹性成像对其行弹性评分。并与术后病理结果对比分析。结果 灰阶超声显示转移性腋窝淋巴结L/S值≤2.0,皮质厚度≥0.3cm,甚至淋巴门消失,与非转移性淋巴结比较差异有统计学意义（P<0.05）。精细血流显像显示淋巴结的血供分布非转移性以Ⅰ型、Ⅱ型为主,转移性以Ⅲ型、Ⅳ型为主,差异有统计学意义（P<0.05）。弹性成像检查非转移性淋巴结弹性分值以1、2分为主,转移性淋巴结弹性分值以3、4分为主,差异有统计学意义（P<0.05）。3种检查方式联合使用诊断准确度较任意单一检查方式均提高,差异有统计学意义（P<0.05）。结论 灰阶超声、精细血流显像和弹性成像技术3种检查方式联合应用明显提高了超声对乳腺癌腋窝淋巴结转移诊断的准确性。     

临床护士心理弹性与职业倦怠的关系研究

目的 分析临床护士心理弹性状况以及与职业倦怠的关系,从心理弹性的角度探讨临床护士职业倦怠,为其心理干预提供依据.方法 采用整群随机方法,对600名临床护士进行心理弹性问卷和职业倦怠量表调查,以多元线性回归进行分析.结果 临床护士总体心理弹性处于中等水平（2.42±0.49）,从各维度来看,坚韧处于中等水平（2.56±0.52）,自强、乐观处于中等偏下水平（2.26±0.43和2.09±0.45）;临床护士不同转班方式、学历、医院等级、年龄、工龄、婚姻、用工性质影响心理弹性总分和部分维度（P＜0.01）;心理弹性总分和各维度与职业倦怠各维度显著相关,心理弹性可以预测职业倦怠水平（P＜0.01）.结论 临床护士总体心理弹性处于中等水平,有待提高.心理弹性对临床护士职业倦怠具有较大的影响,良好的心理弹性更有可能具有较强的抗压能力,更有可能体验较低水平的职业倦怠.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.