高盐饮食对盐敏感性高血压大鼠肾脏损伤的影响

目的 研究高盐饮食对盐敏感性高血压大鼠肾脏组织损伤的影响。方法 将20只实验大鼠按照大鼠品系分为对照组（SS-13^BN大鼠,n=10）与盐敏感性高血压大鼠模型组（Dahl/SS大鼠,n=10）,通过喂食盐含量为8%的高盐饲料构建盐敏感性高血压大鼠模型,分析两组大鼠肾脏组织损伤程度及肾脏组织中白介素-6（IL-6）、白介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的表达情况。结果 模型组大鼠的收缩压为（183.82±3.87）mmHg,对照组为（129.03±3.94）mmHg,差异有统计学意义（P<0.05）。模型组大鼠肾脏组织中Nephrinuria含量为（12.12±0.92）mg/d、肾损伤程度评分为（1.93±0.52）分、TNF-α含量为（623.14±43.12）pg/mg,均高于对照组,差异有统计学意义（P<0.05）。模型组大鼠肾脏组织中IL-6 mRNA的相对表达量为（2.25±0.81）、IL-1β mRNA为（2.89±0.86）,均高于对照组,差异有统计学意义（P<0.05）。模型组大鼠肾脏组织中IL-6蛋白相对表达量为（1.71±0.08）、IL-1β为（2.23±0.13）,均高于对照组,差异有统计学意义（P<0.05）。结论 高盐摄取会对盐敏感大鼠的肾脏造成严重的损伤,引起炎症反应,从而影响肾小球等肾脏器官的正常功能。     

男性年龄与精液常规参数及精子CFTR表达的关系研究

目的 探讨男性年龄与精子质量及精子中CFTR表达的关系。方法 选取2011年3月~8月于笔者医院生殖中心门诊就诊的精液常规参数正常的健康可育男性293例,按照年龄分为3组,A组20~29岁,105例;B组30~39岁,156例;C组≥40岁,32例。进行精液常规分析及精液形态学分析,精子CFTR免疫荧光染色。分析男性年龄与精子质量及CFTR表达间的关系。结果 3组精液：体积分别为A组2.56±0.95ml,B组2.45±0.98ml,C组2.28±0.85ml,前向活动力分别为A组77.91%±17.42%,B组77.71%±14.94%,C组77.68%±15.71%,正常形态率分别为A组7.04%±4.52%,B组6.36%±4.31%,C组5.40%±4.22%,精子浓度分别为A组（106.78±65.33）×10⁶/ml,B组（111.85±65.70）×10⁶/ml,C组（134.59±75.30）×10⁶/ml,精子总数分别为A组（291.3±187.9）×10⁶,B组（256.9±143.4）×10⁶,C组（264.4±170.4）×10⁶,CFTR表达率分别为A组34.58%,B组28.32%,C组23.15%,所有常规参数3者间比较差异均无统计学意义（P>0.05）,组间两两比较差异亦无统计学意义（P>0.05）;相关分析显示精子体积、向前活动力、正常形态率、浓度、总数及CFTR表达率均与年龄无关（P>0.05）。结论 随着年龄的增长,精液体积、前向活动力、正常形态率呈下降趋势,精子浓度呈升高趋势,精子总数无明显变化,但均与年龄无相关性。     

β1受体阻滞剂上调肠道上皮细胞HO-1对脓毒症大鼠肠道功能的保护作用

目的 探讨β₁受体阻滞剂艾司洛尔（Esmolol）能否上调血红素氧合酶-1（heme oxygenase-1,HO-1）起到对脓毒症大鼠肠道功能的保护作用。方法 本实验于哈尔滨医科大学附属第一医院外科中心实验室完成。40只雄性Wistar大鼠按随机数字表法分为4组：假手术组（sham组,n=10）、脓毒症组（CLP组,n=10）、艾司洛尔干预组（Esmolol+CLP组,n=10）、HO-1抑制剂组（Esmolol+ZnPP+CLP组,n=10）。采用盲肠结扎穿孔术（CLP）制备脓毒症大鼠模型,Esmolol组通过静脉输注艾司洛尔注射液,速度为15mg/（kg·h）,Esmolol+ZnPP+CLP组术前1h腹腔内注射ZnPP溶液（40μmol/kg）,术后同速静脉输注艾司洛尔注射液。其余各组大鼠腹腔注射等体积生理盐水,并且静脉输注等渗盐水,速度2ml/（kg·h）。术后12h处死大鼠,ELISA法检测各组血清肿瘤坏死因子（TNF-α）和白细胞介素（IL-1β）水平,采用蛋白印迹法及免疫组化法检测大鼠肠组织HO-1表达水平,光学显微镜观察大鼠肠组织病理变化情况。结果 与Sham组相比,CLP组大鼠血清TNF-α、IL-1β水平均明显升高（43.71±6.24pg/ml vs 2742.69±221.71pg/ml,52.69±14.15pg/ml vs 482.73±125.49pg/ml,P<0.05）;肠组织中HO-1水平表达升高（P<0.05）;肠组织病理损伤明显。与CLP组相比,Esmolol+CLP组血清TNF-α、IL-1β水平均明显下降（2742.69±221.71pg/ml vs 968.81±99.46pg/ml,482.73±125.49pg/ml vs 156.15±38.29pg/ml,P<0.05）;肠组织HO-1水平表达明显升高（P<0.05）;肠组织病理损伤程度减轻。与CLP组相比,Esmolol+ZnPP+CLP组大鼠血清TNF-α、IL-1β水平无明显差异（2742.69±221.71pg/ml vs 2545.18±173.74pg/ml,482.73±125.49pg/ml vs 474.43±113.98pg/ml,P>0.05）;肠组织病理损伤程度无明显差异。结论 Esmolol能改善脓毒症诱发的肠道损伤,其可能是通过上调HO-1通路来减轻肠组织炎性反应,继而减轻肠道损伤。     

咖啡因对大鼠神经行为学与海马形态学及神经元内γ-氨基丁酸和5-羟色胺表达的影响

【目的】 观察不同剂量咖啡因对大鼠神经行为学与海马形态学及神经元内γ-氨基丁酸(GABA)和5-羟色胺表达的影响,以探讨咖啡因改善学习记忆能力的可能作用机制。 【方法】 将30只雌性SD大鼠按完全随机分组法分成3组,对照组(n=10)每天给予生理盐水灌胃,实验组分低剂量组(n=10)和高剂量组(n=10),每天分别给予 20、60 mg/kg咖啡因灌胃,连续18 d,进行咖啡因干预处理。通过Morris水迷宫实验测试各组大鼠的学习记忆能力,运用免疫组织化学方法检测各组大鼠海马神经元内GABA和5-羟色胺表达变化,借助H-E染色、尼氏染色和电子显微镜观察各组大鼠海马神经元形态结构改变。 【结果】 Morris水迷宫实验结果显示咖啡因可以改善大鼠学习记忆能力,高剂量咖啡因作用更加显著,差异具有统计学意义(P<0.05);免疫组织化学染色显示高剂量组(0.214 1±0.006 6)和低剂量组(0.279 4±0.008 1)海马神经元内GABA表达较对照组(0.355 1±0.011 7)减少(F=172.603,P<0.05);高剂量组(0.551 3±0.017 8)和低剂量组(0.485 6±0.008 5)5-羟色胺表达较对照组(0.290 3±0.009 7)增多(F=289.541,P<0.05),且高剂量组变化更加明显;H-E染色未见异常;尼氏染色显示高剂量组(0.559 8±0.009 5)和低剂量组(0.575 9±0.004 9)海马神经元内尼氏体较对照组(0.385 3±0.008 1)增多(F=31.776,P<0.05),且低剂量组增多更加显著;电镜观察发现实验组海马神经元内游离核糖体密度较对照组明显增高,高剂量组海马部分神经元局部内质网轻度扩张、线粒体肿胀、神经髓鞘松解受损。 【结论】 在一定剂量范围内,咖啡因可剂量依赖性地改善大鼠学习记忆能力,其作用机制可能与抑制海马神经元内GABA表达和促进5-羟色胺表达有关。     

外周血中性粒细胞与淋巴细胞比值对慢性鼻窦炎患者诊断及术后复发判断的价值

目的 探讨外周血中性粒细胞数与淋巴细胞数比值（NLR）对慢性鼻窦炎（CRS）患者的诊断及术后复发判断的价值。方法 收集2014年6月至2017年1月在合肥市第三人民医院就诊并确诊为CRS患者67例,作为CRS组。同时,严格筛选同期健康体检者109例作为对照组。采集空腹外周血进行白细胞、中性粒细胞、淋巴细胞计数。术后6个月再次对以上患者进行血细胞计数,并将患者分为术后复发组和术后非复发组,对CRS组、术后复发组、术后非复发组及对照组的上述血细胞指标进行分析。结果 CRS组白细胞计数（7.59±2.47）×10⁹/L、中性粒细胞计数（5.91±2.14）×10⁹/L及NLR水平（5.37±1.80）高于对照组,淋巴细胞计数（1.13±0.37）×10⁹/L低于对照组,差异均具有统计学意义（P<0.05）。ROC曲线分析显示,NLR预测CRS的最佳诊断值为4.37（95%CI：0.931～0.977,P<0.05）,ROC曲线下面积为0.954。方差分析表明,术后未复发组、对照组及CRS组3组间白细胞计数、中性粒细胞计数及NLR水平存在差异,差异具有统计学意义（P<0.05）。术后复发组白细胞计数（8.14±2.92）×10⁹/L、中性粒细胞计数（6.60±2.57）×10⁹/L及NLR水平（6.74±1.88）高于术后未复发组,淋巴细胞计数（1.00±0.36）×10⁹/L低于术后未复发组,差异均具有统计学意义（P<0.05）。结论 外周血NLR在CRS患者的诊断及术后复发评价中具有重要价值。     

胸腺五肽对新辅助化疗的乳腺癌患者局部组织中浸润淋巴细胞的影响

目的 探讨胸腺五肽对新辅助化疗的乳腺癌组织微环境中浸润淋巴细胞的影响。方法 自2012年1月~2014年1月,前瞻性收集笔者医院收治的接受新辅助化疗的乳腺癌患者80例,将患者随机分为研究组和对照组,所有患者均行新辅助化疗,研究组在新辅助化疗期间给予胸腺五肽,化疗结束后进行手术切除肿瘤组织,留取乳腺癌组织标本,观察乳腺癌组织中和外周血中CD4⁺T细胞、CD8⁺T细胞、CD4⁺/CD8⁺T细胞比例、外周血中IL-6、IL-10、TGF-β1水平。结果 与对照组比较,研究组患者乳腺癌组织中CD4⁺T细胞显著降低（21.47%±3.29% vs 23.88%±3.43%,P=0.002）;CD8⁺T细胞显著增高（30.36%±4.38% vs 28.43%±4.16%,P=0.047）;CD4⁺/CD8⁺T细胞显著降低（0.71±0.14 vs 0.84±0.18,P=0.001）。两组患者治疗前外周血CD4⁺T细胞、CD4⁺/CD8⁺T细胞、IL-6、IL-10、TGF-β1等比较,差异均无统计学意义（P>0.05）。与对照组比较,研究组患者术后外周血CD4⁺T细胞水平显著增加（27.67%±4.24% vs 25.09%±3.68%,P=0.005）;CD4⁺/CD8⁺T显著增加（0.83±0.14 vs 0.75±0.18,P=0.029）;IL-6水平显著增高（12.39±2.87 vs 9.24±3.38ng/L,P=0.000）;IL-10水平显著降低（8.49±2.59 vs 12.59±3.65ng/L,P=0.006）;TGF-β1水平显著降低（12.47±3.88 vs 17.76±4.12ng/ml,P=0.000）。两组患者住院时间、切口感染、手术部位出血、复发率和病死率等比较,差异均无统计学意义（P > 0.05）。结论 胸腺五肽联合新辅助化疗可能有助于改善乳腺癌组织微环境中免疫状态和全身免疫功能。     

姜黄素对实验性大鼠非酒精性脂肪肝病氧化应激、炎性因子及细胞凋亡水平的影响

目的 探讨姜黄素对实验性大鼠非酒精性脂肪肝病肝脏氧化应激、炎性因子及细胞凋亡等水平的影响并初步探讨其机制.方法 将大鼠随机平均分为正常对照组(NC组)、高脂饮食组(HF组)和高脂饮食+姜黄素治疗组(HF+curcumin组),各组给予相应处理造模后,观察肝脏病理变化并分析相关血清学指标.结果 NC组、HF组和HF+curcumin组大鼠血GSH-Px、T-AOC、SOD分别为28.5±2.5、21.7±3.1、24.1±1.2;4.3±0.9、1.8±1.1、2.9±0.7;163.7±13.4、123.5±21.1、143.1±16.7,MAD分别为1.3±0.5、3.2±0.8、2.4±0.6,血IL-6、TNF-α分别为67.5±12.4、114.5±15.2、91.3±16.1;32.8±5.5、40.5±4.2、34.5±6.1,组间比较差异均有统计学意义(P<0.05或P<0.01);各组间NF-κB/GAPDH及Bcl-2/Bax比值差异均有统计学意义(P<0.05或P<0.01).结论 姜黄素能够减轻NAFLD大鼠肝脏脂肪变性程度,改善胰岛素抵抗、肝功能及病理损伤,明显减轻其发病关键环节氧化应激,炎性反应及细胞凋亡的程度.     

70岁及以上非肥胖腹股沟疝患者临床路径预防性使用抗菌药物的临床效果

目的 探讨非肥胖腹股沟疝患者（≥70岁）围手术期是否使用预防性抗菌药物在临床路径（CP）中的效果。方法 选择合肥市第三人民医院普外科2017年2月至2019年12月CP中控制好合并症的76例非肥胖高龄腹股沟疝患者,按照随机数字表法,分为试验组（38例）与对照组（38例）。试验组患者不使用抗菌药物,对照组患者使用预防性抗菌药物。分析两组患者术后切口情况及相关指标。结果 试验组患者术后切口并发症发生率为7.89%,对照组为5.26%,两组差异无统计学意义（P>0.05）。两组患者术前白细胞计数[（5.52±1.28）×10⁹/L vs（5.73±1.59）×10⁹/L]、中性粒细胞比值[（63.91±6.55）% vs（63.77±9.18）%]、C反应蛋白（CRP）[（3.35±0.80）mg/L vs（3.30±0.79）mg/L]及最高体温[（36.59±0.44）℃ vs（36.62±0.36）℃]进行比较,差异无统计学意义（P>0.05）。两组患者术后白细胞计数[（5.98±1.37）×10⁹/L vs（6.11±1.66）×10⁹/L]、中性粒细胞比值[（65.22±6.49）% vs（64.98±9.09）%]、CRP[（7.47±0.85）mg/L vs（7.13±0.86）mg/L]及最高体温[（36.84±0.45）℃ vs（36.81±0.34）℃]进行比较,差异无统计学意义（P>0.05）。两组患者手术前后白细胞计数、中性粒细胞比值、CRP及最高体温差值进行比较,差异无统计学意义（P>0.05）。。结论 对于CP中控制好合并症的非肥胖高龄腹股沟疝患者,围手术期可以不使用预防性抗菌药物。     

星状神经节阻滞术对大鼠急性心肌梗死后炎症反应的影响

目的 探讨星状神经节阻滞术（SGB）对大鼠急性心肌梗死（AMI）后心肌细胞炎症的保护作用。方法 选取8周龄雄性SPF级Wistar大鼠60只,体重200～250 g,随机分为假手术组、AMI组和SGB组,每组20只。假手术组只打开胸腔暴露心脏,不结扎冠状动脉;AMI组结扎冠状动脉左前降支复制AMI模型;SGB组在AMI模型复制成功后行SGB。模型复制成功后1周采集各组大鼠血清及心脏组织,qRT-PCR检测各组大鼠心肌组织中炎症因子（IL-6、IL-1β及TNF-α）mRNA;酶联免疫吸附试验检测各组大鼠血清中炎症因子（IL-6、IL-1β及TNF-α）,全自动生化分析仪检测各组大鼠血清中心肌酶（AST、LDH和CK-MB）。结果 各组大鼠心肌组织中炎症因子IL-6、IL-1β及TNF-α mRNA相对表达量比较,差异有统计学意义（P <0.05）,SGB组IL-6、IL-1β及TNF-α mRNA的相对表达量均较AMI组降低（P <0.05）。各组大鼠血清中炎症因子IL-6、IL-1β及TNF-α表达水平比较,差异有统计学意义（P <0.05）,AMI组、SGB组IL-6、IL-1β及TNF-α表达水平较假手术组升高（P <0.05）,SGB组IL-6、IL-1β及TNF-α表达水平较AMI组降低（P <0.05）。各组大鼠血清AST、LDH及CK-MB表达水平比较,差异有统计学意义（P <0.05）,AMI组、SGB组AST、LDH及CK-MB表达水平较假手术组升高（P <0.05）,SGB组AST、LDH和CK-MB表达水平较AMI组降低（P <0.05）。结论 SGB对大鼠AMI后心肌细胞炎症有一定的保护作用。     

姜黄素对沙漠干热环境大鼠生存率影响的实验

目的 探讨姜黄素对沙漠干热环境下大鼠生存率的影响。方法 选择SPF级6～8周龄雄性SD大鼠40只,随机分为对照组、姜黄素低剂量预处理组、姜黄素中剂量预处理组、姜黄素高剂量预处理组、溶剂组。对照组,正常饲喂;低剂量组、中剂量组、高剂量组、溶剂组分别等体积灌胃连续7 d。置于西北地区特殊环境人工实验舱,设定沙漠干热环境气候模式(温度41℃,湿度10%,紫外线辐射度40W/m²)。观察各组大鼠机体的生命体征并每半小时监测核心体温变化,记录死亡时间。结果 五组大鼠从被置于沙漠干热环境开始,0～30 min,姜黄素三个剂量组的核心体温与对照组、溶剂组比较无显著差异(P>0.05)。从60 min开始,60 min、90 min、120 min、150 min、180 min、210 min、240 min7个时间点,低剂量组、中剂量组、高剂量组的核心体温低于对照组、溶剂组,差异有显著性(P<0.05)。从0～240 min各时间点低剂量组、中剂量组、高剂量组组间比较均无显著性差异(P>0.05)。对照组、姜黄素低、中、高剂量治疗组及溶剂组五组大鼠平均生存时间分别为(222.95±28.23)min、(231.35±40.96)min、(255.00±30.39)min、(263.80±56.71)min、(223.00±27.23)min。中、高剂量姜黄素预处理组大鼠的生存时间均明显长于低浓度预处理组、对照组和溶剂组(P<0.05),其中高剂量组效果显著(P<0.01)。结论 姜黄素预处理能够提高沙漠干热环境大鼠的生存时间,提示姜黄素可能在提高沙漠干热环境大鼠耐热性方面发挥重要作用,姜黄素可能在沙漠干热环境中暑的防治方面具有潜在的临床应用价值。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.