提高血氧分压对不同储存期血液红细胞影响的探讨

目的：探讨提高血氧分压在4℃保存条件下对不同储存期红细胞的影响。方法：在无菌条件下，分别对采集0d，7d，14d，21d的库存血进行无菌氧处理以提高血氧分压。分实验组和对照组，测定血液红细胞在提高血氧分压后的血浆游离Hb浓度，血浆K^＋离子浓度，红细胞超氧化物歧化酶（SOD）活性，谷胱甘肽过氧化物酶（GSH—Px）活性。结果：与对照组比较，在4℃保存条件下，血液红细胞在提高血氧分压初期（1—2d），血浆游离Hh浓度和血浆K^＋离子浓度无明显变化（p〉0．05），红细胞SOD活性、红细胞GSH—Px活性有所提高（p〈0．05）；随着储存时间的延长，血浆游离Hb浓度增加、血浆K^＋离子浓度增高（p〈0．01），红细胞SOD活性和红细胞GSH—Px活性降低（p〈0．01）。结论：（1）不同储存期红细胞能保持较高血氧分压和血氧保和度；（2）血液红细胞在提高血氧分压的初期不会产生明显的溶血反应和其它损伤，红细胞抗氧化酶活性有所提高。随着储存时间的延长，红细胞抗氧化酶活性下降，溶血反应加重，存在红细胞膜氧化损伤作用，这与氧自由基攻击红细胞膜发生过氧化反应及红细胞抗氧化酶活性降低有关。     

SOD强化刺梨汁对冠心病患者抗脂质过氧化作用的研究

本文报道对31例老年冠心病患者服用SOD强化刺梨汁前后血浆丙二醛(MDA)含量、红细胞超氧化物歧化酶(SOD)和硒谷胱甘肽过氧化物酶(SeGSH-Px)活性的测定。结果表明,服药后与服药前比较,血浆MDA含量显著降低(P<0.05);红细胞SOD活性升高,但无显著性(P>0.05);SeGSH-Px活性明显升高(P<0.01),提示SOD强化刺梨汁具有不同程度地升高两种抗氧化酶活性的作用,能明显抑制冠心病患者体内的脂质过氧化作用。     

癫痫患者血浆及红细胞中抗氧化剂水平的测定

目的：探讨癫痫疾病状态下血浆及红细胞（RBC）中抗氧化剂水平的变化。方法：测定39例正常对照组、32例癫痫疾病组和26例癫痫治疗组的RBC内谷胱甘肽还原酶(GR)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)、红细胞膜过氧化脂质代谢产物丙二醛（MDA）、红细胞溶血百分率以及血浆中铜蓝蛋白（CER）、维生素A,C,E(VitA、VitC、VitE)等。结果：疾病组的红细胞MDA,溶血百分率,GSH-Px,CAT及血浆CER显著高于对照组（P<0.05），红细胞GR、SOD和血浆VitA，VitC，VitE显著低于对照组（P<0.05,其中红细胞GR，血浆维生素C,P<0.01）；治疗组的红细胞GR,SOD，血浆VitC,VitE均显著高于疾病组(P<0.05,其中GR,P<0.01)。结论：提示采取抗氧化保护措施对患者的治疗有重要意义。     

癫痫患者血浆及红细胞中抗氧化剂水平的测定

目的：探讨癫痫疾病状态下血浆及红细胞（RBC）中抗氧化剂水平的变化。方法：测定39例正常对照组、32例癫痫疾病组和26例癫痫治疗组的RBC内谷胱甘肽还原酶(GR)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)、红细胞膜过氧化脂质代谢产物丙二醛（MDA）、红细胞溶血百分率以及血浆中铜蓝蛋白（CER）、维生素A,C,E(VitA、VitC、VitE)等。结果：疾病组的红细胞MDA,溶血百分率,GSH-Px,CAT及血浆CER显著高于对照组（P<0.05），红细胞GR、SOD和血浆VitA，VitC，VitE显著低于对照组（P<0.05,其中红细胞GR，血浆维生素C,P<0.01）；治疗组的红细胞GR,SOD，血浆VitC,VitE均显著高于疾病组(P<0.05,其中GR,P<0.01)。结论：提示采取抗氧化保护措施对患者的治疗有重要意义。     

表没食子儿茶素没食子酸酯对缺血再灌注损伤大鼠心肌脂质过氧化及心肌酶活性的影响

目的：观察表没食子儿茶表没食子酸酯（EGCG）对缺血再灌注损伤大鼠心肌脂质过氧化及心肌酶活性的影响。方法：雄性Wistar大鼠50只分为5组，假手术组、缺血再灌注组（IR），丹参（SM，100mg/kg）组，EGCG1（10mg/kg)组和EGCG2（20mg/kg）组，每组10只动物，采用结扎大鼠左冠状动脉前降支30min后再灌注60min的方法建立在体缺血再灌注损伤模型，分别测定心肌丙二醛（MDA）含量，超氧化物歧化酶（SOD）及ATP酶活性和血浆乳酸脱氢酶（LDH）水平。结果：与假手术组比，IR组大鼠心肌MDA及血浆LDH明显升高，SOD及ATP酶活性则显著降低（P＜0．01）；EGCG和SM均能显著，降低MDA与LDH，明显增加SOD与ATP酶活性（与IR组比，P＜0．01），并且EGCG（20mg/kg)，降低MDA与LDH的作用较SM（100mg/kg)更明显（P＜0．05）。结论：EGCG通过抑制脂过氧化反应和提高心肌SOD与ATP酶活性，对大鼠缺血再灌注损伤心肌有显著的保护作用。     

通心络治疗冠心病疗效观察及其机制初步探讨

目的 观察通心络胶囊对25例冠心病患者的疗效，并探讨其作用机制。方法 45例冠心病患者随机分为通心络治疗组23例及对照组（常规治疗）22例，观察治疗前后临床症状心电图、胆固醇、甘油三脂、低密度脂蛋白、血浆纤维蛋白原、血浆丙二醛（MDA）及红细胞超氧化物歧化酶（SOD）等指标，并进行评估。结果 经过4周治疗后，通心络治疗组临床症状、心电图、胆固醇、血浆纤维蛋白原等指标的改善优于对照组，血浆丙二醛（MDA）含量降低比对照组明显，而红细胞超氧化物歧化酶（SOD）活性则明显高于对照组。结论 通心络对冠心病具有较好的疗效，其作用机制可能与该药有保护SOD活性，清除氧自由基，从而减少氧自由基对细胞膜的损伤有关，且能降低血脂，降低纤维蛋白原，对改善血液流变学有利。     

肉桂酸对小鼠运动疲劳的实验研究

目的：探讨肉桂酸对提升小鼠运动耐力的影响。方法通过建立力竭性游泳来建立小鼠力竭性运动模型,比较安静对照组、运动训练组和运动给药组小鼠力竭游泳持续时间,血浆中乳酸（LAC）含量来探讨肉桂酸对小鼠的耐力提升;检测肝脏组织匀浆中超氧化物歧化酶（SOD）、丙二醛（MDA）和过氧化酶（CAT ）的含量来探讨肉桂酸的抗氧化能力,通过Western blot方法检测肉桂酸对小鼠股四头肌中Na＋／K＋‐ATP酶、Ca2＋／Mg2＋‐ATP酶表达水平来进一步研究其对小鼠运动能力提升的影响。结果肉桂酸显著延长小鼠的游泳时间;运动给药组与安静对照组比较：血浆 LAC含量虽然有所升高,但差异无统计学意义（P＞0．05）,肝脏组织匀浆中SOD和CAT 显著下降（P＜0．05）,MDA显著上升（P＜0．01）,CAT 虽下降但差异无统计学意义（P＞0．05）,Na＋／K＋‐ATP酶和Ca2＋／Mg2＋‐ATP酶的表达水平显著下降（P＜0．01）;运动训练组与安静对照组比较,血浆LAC含量显著升高（P＜0．01）,肝脏组织匀浆中SOD和CAT 显著下降（P＜0．01）,MDA显著上升（P＜0．01）,Na＋／K＋‐ATP酶和Ca2＋／Mg2＋‐ATP酶的表达水平显著降低（P＜0．01）;运动给药组与运动训练组比较,血浆LAC含量显著降低（P＜0．01）,肝脏组织匀浆中SOD和CAT显著上升（P＜0．01）,MDA显著下降（P＜0．01）,CAT 显著下降（P＜0．01）,Na＋／K＋‐ATP酶和Ca2＋／Mg2＋‐ATP酶的表达水平显著降低（P＜0．05）。结论肉桂酸具有良好的抗氧化能力和抗疲劳能力。     

增力祛疲口服液对大鼠运动性疲劳的作用

目的：探讨增力祛疲口服液抗运动性疲劳作用的机制。方法：将30只雄性SD大鼠随机分成正常对照组、疲劳对照组和口服液组，每组10只，选用游泳训练导致大鼠运动性疲劳模型，用组织学观察、生化测定和细胞化学分析等方法，研究3组大鼠骨骼肌组织结构和线粒体中脂质过氧化物（LPO）、Ca^2＋、K^＋含量以及血清中睾酮（T）、皮质醇（C）、T／C值、超氧化物歧化酶（SOD）、丙二醛（MDA）、肌红蛋白（Mb）的变化。结果：与正常对照组比较，疲劳对照组大鼠血清中T／C值、SOD、Mb和线粒体中Ca^2＋、K^＋均降低，而血清MDA和线粒体LPO升高（P〈0．05）；与疲劳对照组比较，口服液组大鼠上述指标均改善。结论：该药有利于骨骼肌组织疲劳后的功能恢复，有增强抗疲劳的作用，     

回春汤对D-半乳糖衰老模型小鼠抗氧化系统的影响

目的探讨回春汤对D-半乳糖衰老模型小鼠抗氧化系统的影响。方法将小鼠随机分为空白组、衰老模型组、维生素E组、回春汤大、小刺量组5组，采用灌胃给药6周后测定指标。选用：血浆丙二醛（MDA）含量、红细胞超氧化物歧化酶（S0D）活力、肝组织谷胱甘肽过氧化物酶（Gsh-Px）活力为指标，观察回春汤对D-半乳糖衰老模型小鼠抗氧化系统的影响。结果回春汤2个剂量组均能提高D-半乳糖所致衰老小鼠的红细胞SOD活力、肝组织谷胱甘肽过氧化物酶（GSH—PX）活力，降低血清MDA含量。结论回春汤具有良好的抗氧化作用，有助于延缓衰老过程。     

脑活素对大鼠海马缺血再灌注损伤的影响

本文通过结扎大鼠两则颈总动脉30min后再8灌注1小时，造成海马缺血再灌注损伤模型，观察脑活素对大鼠脑缺血再灌注损伤的影响。海马缺血再灌注时超氧化物歧化酶（SOD）Na^ —K^ ATPase，Ca^ 2—ATPase的活性均降低；丙二醛（MDA）含量增高；预防性应用脑活素可以明显增加总抗氧化活性，降低MDA含量。结果提示脑活素具有抗氧化作用，对脑缺血再灌注损伤具有保护作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.