葡萄酒中白藜芦醇研究进展

综述了近年来关于葡萄酒中白藜芦醇及其衍生物的药理、生物活性,葡萄酒酿造工艺过程中白藜芦醇含量变化及影响因素等方面的研究进展.同时展望了上述研究领域今后预期的主要研究方向.     

白藜芦醇功能和作用机理研究进展

白藜芦醇主要存在于葡萄、虎杖、花生等植物中,是一种天然的抗肿瘤、抗心血管疾病、抗突变、抗菌、抗炎、抗氧化、诱导细胞凋亡及雌激素调节物质。文章介绍了近年来对白藜芦醇生物活性和作用机理方面的研究进展,并展望白藜芦醇在食品方面的开发和应用。     

花生中白藜芦醇的研究现状及应用展望

简要介绍白藜芦醇的理化特性及对疾病预防和治疗作用方面的研究进展,并重点阐述了近年来国内外花生中白藜芦醇的提取分离工艺,分析检测方法的研究现状,为研究和开发花生中白藜芦醇的工作者提供参考,同时展望了花生中白藜芦醇的开发和应用前景.     

发芽处理后花生中白藜芦醇积累情况的高效液相色谱法的研究

花生中白藜芦醇是一种天然存在的生物活性很强的多酚类物质。目的：本实验通过高效液相色谱法研究不同的胁迫试剂提高花生发芽过程中白藜芦醇的积累。方法：通过三种不同的花生发芽培养液(无菌去离子水,600 μM CuSO4溶液和10 mg/L壳聚糖溶液)对花生发芽过程进行处理,并且建立了提取和净化花生中白藜芦醇的前处理方法。结果：不同胁迫试剂处理发芽花生对白藜芦醇的富集影响不同。用无菌去离子水对发芽花生处理中72 h白藜芦醇含量最高为130.2 ng/g,用600 μM CuSO4溶液对发芽花生处理中24 h白藜芦醇含量最高为568.7 ng/g,用10 mg/ L的壳聚糖溶液处理五天后白藜芦醇含量最高为383.6 ng/g。结论：在三种处理方式中,根据发芽产品的需要,用CuSO4和壳聚糖都可以作为胁迫试剂对发芽花生进行处理以此来提高花生不同发芽阶段白藜芦醇的含量。     

白藜芦醇的生物活性及其运载体系研究进展

白藜芦醇是一种主要存在于葡萄、花生、虎杖等植物中的天然多酚,具有抗氧化、抗菌消炎、抗心血管疾病、抗肿瘤等多种生物活性,在食品、药品及日化品领域都有广泛应用。但由于白藜芦醇性质不稳定、水溶性差等特点,限制了其应用范围,因此,利用运载体系提高其生物利用度是一个关键的研究方向。本文总结了白藜芦醇理化性质及生物活性,并对近年来有关白藜芦醇的运载体系进行综述,提出现有运载体系存在的问题,以期为白藜芦醇的运载体系的发展提供参考。     

花生种子及花生植株各部位白藜芦醇含量的测定

为准确测定白藜芦醇在花生种子及花生植株各部位中的含量,通过超声萃取和高效液相色谱法测定花生种子及花生植株各部位的白藜芦醇含量。结果表明,花生植株根部中白藜芦醇含量最高,达到629.487μg/g,花生种子中含量极低,不超过2μg/g;种子发芽处理后,白藜芦醇含量最高,达到28.041μg/g,满足工业提取对白藜芦醇含量的要求,说明花生种子处理后及花生植株对白藜芦醇来源有较大的经济价值。     

花生红衣多酚的研究进展

花生红衣中含有丰富的活性物质,具有很高的营养和利用价值,但在我国作为花生产业副产品的红衣利用率并不高。近年来,其生物活性日益受到人们关注。多酚物质是花生红衣中一种天然大分子化合物,根据对近年来国内外相关资料文献的统计分析,从提取、纯化和生物活性等方面对其研究现状作了介绍,并展望了其应用前景。     

白藜芦醇的提取、检测技术及其生物活性研究进展

介绍白藜芦醇在天然植物中的分布、理化性质、生物活性及其药理作用,阐述近年来国内外有关植物中白藜芦醇的提取与分离纯化、分析测定等方面的研究进展.     

不同处理方式对发芽花生中白藜芦醇积累的影响

目的通过高效液相色谱法研究不同的胁迫试剂提高花生发芽过程中白藜芦醇的积累。方法通过3种不同的花生发芽培养液(无菌去离子水, 600μmol/L CuSO_4溶液和10 mg/L壳聚糖溶液)对花生发芽过程进行处理,并且建立提取和净化花生中白藜芦醇的前处理方法。结果不同胁迫试剂处理发芽花生对白藜芦醇的富集影响不同。用无菌去离子水对发芽花生处理72 h白藜芦醇含量最高为130.2 ng/g,用600μmol/L CuSO_4溶液对发芽花生处理24 h白藜芦醇含量最高为568.7 ng/g,用10 mg/L的壳聚糖溶液处理5 d后白藜芦醇含量最高为383.6 ng/g。结论用CuSO_4和壳聚糖都可以作为胁迫试剂对发芽花生进行处理,可以提高花生不同发芽阶段白藜芦醇的含量。     

花生功能成分及其综合利用

介绍了世界和我国的花生种植和生产情况,综述了花生的功能成分及其特性.对花生油、花生蛋白、花生种皮和花生壳的开发现状进行了较全面的总结,对花生中的磷脂、维生素E、植物甾醇、蛋白质、维生素K、白藜芦醇和粗纤维等功能成分在食品、医药、饲料和化工等行业中的应用前景进行了较详尽的讨论,可为合理有效地综合利用花生资源提供有价值的参考.     

萌芽花生营养成分变化及其功能作用研究进展

花生萌芽过程中,蛋白质发生水解,脂肪含量下降,而白藜芦醇含量急遽增加,可作为理想的功能食品基料。本文统计了近50年关于萌芽花生的文献,重点分析了花生萌芽过程中蛋白质、脂肪等主要营养物质以及白藜芦醇含量的变化,介绍了萌芽花生抗氧化和降脂等功能作用的研究进展,同时还详细阐述了国内外萌芽花生加工利用的研究现状。在现有研究基础上,指出了目前萌芽花生的营养因子富集、功能作用研究等方面存在的问题,并提出萌芽花生理论研究与食品开发等方向的展望,以期为萌芽花生在食品工业中的进一步开发与利用提供参考。     

Metabolism and bioavailability of trans-resveratrol

Resveratrol (3,4',5-trihydroxy-trans-stilbene) is a polyphenolic compound accounting to the stilbene class. Most stilbenes in plants act as antifungal phytoalexins, compounds that are usually synthesized only in response to infection or injury. Resveratrol has been detected in trees, in a few flowering plants, in peanuts, and in grapevines. The major dietary sources of resveratrol include grapes, wine, peanuts, and peanut products. Numerous in vitro studies describe different biological effects of resveratrol. The major impacts are the antioxidative, anti-inflammatory, and estrogenic effects as well as anticancer and chemopreventive activities. In order to reveal information on absorption, metabolism, and the consequent bioavailability of resveratrol, different research approaches were performed, including in vitro, ex vivo, and in vivo models, all of which are considered in this review. Summarizing the data, resveratrol is absorbed and metabolized. Around 75% of this polyphenol are excreted via feces and urine. The oral bioavailability of resveratrol is almost zero due to rapid and extensive metabolism and the consequent formation of various metabolites as resveratrol glucuronides and resveratrol sulfates. The potential biologic activity of resveratrol conjugates should be considered in future investigations.     

Optimisation for resveratrol accumulation during peanut germination with phenylalanine feeding & ultrasound‐treatment using response surface methodology

Germinated peanut has been used as a novel functional food these days, which has almost six times trans‐resveratrol to peanut seeds. Resveratrol is a natural occurring stilbene phytoalexin phenolic compound produced in response to a variety of biotic and abiotic stresses. In this study, we investigated the effects of ultrasonic treatments and phenylalanine feeding simultaneously on enhancing resveratrol during peanut germination. The influences of germinal time, phenylalanine concentration as well as ultrasound frequency, treatment temperature and time on resveratrol accumulation in germinated peanut were studied, respectively. Subsequently, based on Box–Behnken design, interactive effects of the phenylalanine feeding & ultrasound parameters were evaluated. The optimum conditions for resveratrol accumulation were as follows: phenylalanine concentration at 0.8 mm , ultrasonic power at 240 W, ultrasonic treatment time at 30 min and ultrasonic treatment temperature at 35 °C. Using the optimum condition, resveratrol concentration in germinated peanuts reached 36.99 μg g^?1 (DW), which was almost 9.4 times higher than that in the nongerminating peanut.     

HPLC法测定不同品种花生根中白藜芦醇的含量

本研究采用高效液相色谱法(HPLC)测定河南产花育20、豫花11、豫花14、豫花15、红花1号、海花1号、丰花5号、白沙17A共八种花生根中白黎芦醇的含量,结果表明:这八种花生品种中以红花1号花生根中白黎芦醇含量最高,为103-3μg/g,不同品种花生根中白黎芦醇含量差异比较大。     

大孔吸附树脂纯化花生根中白藜芦醇工艺及其动力 学研究

以花生根白藜芦醇提取液为原料,对选取的5种大孔树脂进行静态吸附试验,确定DA-201树脂为最优吸附树脂.通过DA-201树脂吸附白藜芦醇的动力学试验、DA-201树脂等温吸附试验、上样量试验与动态洗脱试验以及考察上样流速、洗脱流速和洗脱溶剂浓度的三元二次通用旋转组合设计柱层析试验等研究发现:DA-201树脂等温吸附白藜芦醇过程符合Langmuir和Freundilch方程.在上样质量浓度为0.7 mg/mL,上样液pH 3,上样体积为20 mL,洗脱体积为15 mL的条件下,进行DA-201大孔吸附树脂柱层析纯化试验,建立了大孔吸附树脂柱层析纯化白藜芦醇的数学模型.经回归与方差分析,对方程进行局部寻优得出:在上样流速1.00 mL/min,洗脱流速1.60 mL/min,乙醇体积分数75%,其纯化后白藜芦醇的得率为(80.13±0.01)%,经HPLC检测其纯度可以达到39.61%.     

响应面法优化花生根中白藜芦醇提取工艺研究

为了探索花生根白藜芦醇提取的最佳条件,在单因素试验的基础上,应用响应面法优化花生根白藜芦醇的提取条件。结果表明：乙醇浓度、提取温度和提取时间对花生根白藜芦醇的提取效果有显著影响,且为非线性关系,最佳提取条件为乙醇浓度65%、提取温度52℃、提取时间39min,在此条件下白藜芦醇的提取率为0.012%。实验证明响应面法对花生根白藜芦醇提取条件的优化是可行的,得到的白藜芦醇提取条件具有实际应用价值。     

反相高效液相色谱法测定不同品种花生白藜芦醇含量

以珍珠红1号、花育20、花育22、花育31号为原料,采用反相高效液相色谱法(RP-HPLC)测定花生各部位中白藜芦醇含量。测定结果表明,不同品种花生其白藜芦醇含量不同,且花生各部位含量也有所不同;其中珍珠红1号花生根白藜芦醇含量最高,为90.37μg/g。该法回收率98.08%,RSD 2.01%,操作简便、结果准确可靠。     

Differential effects of resveratrol and its naturally occurring methylether analogs on cell cycle and apoptosis in human androgen‐responsive LNCaP cancer cells

Stilbenes are phytoalexins that become activated when plants are stressed. These compounds exist in foods and are widely consumed. Resveratrol is a grape‐derived stilbene, which possesses a wide range of health‐promoting activities, including anticancer properties. Several other stilbenes structurally similar to resveratrol are also available in food, but their biological activities remain largely unknown. In this study, we compared the effects of resveratrol and its natural derivatives pterostilbene, trans‐resveratrol trimethylether, trans‐pinostilbene and trans‐desoxyrhapontigenin on androgen‐responsive human prostate cancer LNCaP cells. We found that these compounds exert differential effects on LNCaP cell growth, cell cycle and apoptosis. Trans‐resveratrol trimethylether appeared to be the most potent compound among the stilbenes tested. Treatment of LNCaP cells with trans‐resveratrol trimethylether resulted in G2/M blockage while other compounds, including resveratrol, induced G1/S arrest. Moreover, different from other compounds, trans‐resveratrol trimethylether induced apoptosis. At the molecular level, the effects of these compounds on cell cycle correlated with induction of the cyclin‐dependent kinase inhibitor 1A and B mRNA levels. Additionally, these compounds also inhibited both androgen‐ as well as estrogen‐mediated pathways. These results provide mechanistic information on how resveratrol and its methylether analogs may act to contribute to potential antiprostate cancer activity.     

超声提取花生根中白藜芦醇的工艺研究

以乙醇溶液为溶剂,对超声提取花生根中白藜芦醇的工艺进行了研究。首先,建立了白藜芦醇含量测定的紫外分光光度法。然后,以白藜芦醇的提取率为考察指标,考察了不同乙醇浓度、提取时间、提取温度、料液比对花生根中白藜芦醇提取工艺的影响。通过单因素试验和正交试验确定了最佳超声提取条件:乙醇溶液浓度为80%,提取时间为40min,提取温度为50℃,料液比为m(花生根质量,g):υ(乙醇溶液体积,ml)=1:10。此时白藜芦醇得率为0.192‰。