正交函数分光光度法测定复方头孢氨苄胶囊中头孢氨苄含量

本文采用正交函数分光光度法，以二次正交多项式（ｊ＝２），波长点数Ｎ＝６，波长间隔△λ＝４ｎｍ，中间波长λｍ＝２６６．４ｎｍ，测定复方头孢氨苄胶囊中头孢氨苄的含量．方法平均回收率为９９．２％（ＲＳＤ＝０．６％，ｎ＝９），与现行药品标准方法比较，结果不存在显著性差异（Ｐ＝０．９５）．     

反相高效液相色谱法测定清热解毒口服液中盐酸小檗碱的含量

本文建立了用反相高效液相色谱法测定清热解毒口服液中盐酸小檗碱含量的方法。在ＺｏｒｂａＯＤＳ－Ｃ１８柱上，以０．０２Ｍ磷酸二氢钾－乙腈（６０：４０）为流动相，检测波长为３４６ｎｍ。加样回收率为９９．７８％，ＲＳＤ为２．４６％（ｎ＝５），本法简便、准确，其它组分对测定无干扰。     

双波长分光光度法测定复方替硝唑含漱液中替硝唑及醋酸洗必泰的含量

本文应用双波长分光光度法，在２３１ｎｍ、２８４．５ｎｍ和３１７ｎｍ波长处，以硼砂缓冲液（ｐＨ８．２）作溶剂，直接测定替硝唑和醋酸洗必泰的含量，方法简便，结果准确。回收率分别为１００．６２％和９９．１０％，ＲＳＤ分别为０．８９％和０．５７％。     

双波长分光光度法测定复方替硝唑含嗽液中替硝唑及醋酸洗必…

本文应用双波长 光度法，在２３１ｎｍ、２８４．５ｎｍ和３１７ｎｍ波长处，以硼硝缓冲液（ＰＨ８．２）作溶剂，直接测定替硝唑和醋酸洗必泰的含量，方法简便，结果准确。回收率分别为１００．６２％和９９．１０％；ＲＳＤ分别为０．８９％和０．５７％。     

分光光度法同时测定复方呋喃西林滴鼻液中两组分的含量

采用分光光度法同时测定复方呋喃西林滴鼻液中盐酸麻黄碱和呋喃西林（ＵＶ法）的含量，盐酸麻黄碱的测定波长为２５６．０ｍｍ，参比波长为３７３．６ｍｍ，Ｋ系数为２．４７７，平均回收率为９９．８０％，ＲＳＤ＝０．２２％。呋喃西林测定波长为３７３．６ｎｍ，平均回收率为９９．４０％。     

紫外分光光度法对曲尼司特胶囊的含量测定

曲尼司特胶囊为一新的平喘药。其含量测定常用薄层色谱法和中和法。本文采用紫外分光光度法对曲尼司特胶囊进行定量测定。测定波长３３４ｎｍ，结果回收率为９９．４６％，ＲＳＤ为１．８７％（ｎ＝５），标准曲线呈良好的线性（ｒ＝０．９９９９）。本方法简捷、准确。     

紫外分光光度法测定复方甲硝唑滴耳液中甲硝唑和诺氟沙星的含量

采用差示分光光度法和单波长分光光度法，在３２０ｎｍ和２７７ｎｍ波长处，测定复方甲硝唑滴耳液中甲硝唑和诺氟沙星的含量。回收率分别为９９．５４％（ＲＳＤ＝０．５０％）和１００．１０％（ＲＳＤ＝０．４２％）。此法适宜于该制剂质量控制。     

紫外分光光度法测定丙硫氧嘧啶片的含量

采用紫外分光光度法测定丙硫氧嘧啶片的含量,测定波长为２７４ｎｍ,其方法线性范围为１～９μｇ／ｍＬ,平均回收率为９９．８％,ＲＳＤ为０．５％（ｎ＝６）。     

用HPLC法测定至灵胶囊中腺苷的含量

采用高效液相色法测定至灵胶囊中腺苷的含量，选用ＹＷＧ－Ｃ１８层，磷酸二氢钾（０．００５ｍｏｌ／Ｌ）甲醇（８５：１５）为流动相，检测波长为２６０ｎｍ平均回收率为９９．４４％，Ｒｓ为１．２８％（ｎ＝５）。     

双波长分光光度法测定心脏基础液中L—苹果酸的含量

本文采用双波长分光光度法测定心脏基础液中Ｌ－苹果酸的含量，测定波长为３７０．０ｍｍ，参比波长为４６８．０ｎｍ平均回收率为９９．４５％，ＲＳＤ为０．４３％，该法快速，简便，结果准确，适用于该制剂质量控制。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.