祛腐生肌膏促进感染创面愈合作用的实验研究

目的观察祛腐生肌膏对感染创面的作用,为中医古方和临床用药提供科学依据。方法采用新西兰大白兔12只,制作感染创面动物模型,形成36个创面,随机分为实验组、药物对照组和空白对照组,每组12个创面。实验组用祛腐生肌膏,药物对照组用红霉素软膏,空白对照组用生理盐水进行换药治疗,分别于伤后7天在创面肉芽组织取材,测定肉芽组织蛋白质含量,计数成纤维细胞、毛细血管,检查脓液中吞噬细胞数,记录创面愈合率、愈合时间。结果祛腐生肌膏可以促进创面成纤维细胞及毛细血管的增殖（P〈0.01）;增加创面蛋白质含量（P〈0.01）,促进肉芽组织生长,提高创面愈合率（P〈0.01）,缩短创面愈合时间（P〈0.01）。结论祛腐生肌膏具有促进感染创面愈合的作用,其机制包括促进组织修复细胞和炎性细胞的增殖,促进肉芽组织生长。     

凉血解毒油治疗Ⅱ°烧伤45例临床疗效观察

目的探讨凉血解毒油对Ⅱ°烧伤创面治疗的临床疗效.方法将90例Ⅱ°烧伤患者随机分为两组,治疗组45例应用凉血解毒油治疗,对照组45例采用京万红油膏治疗.两组均采用暴露或包扎疗法,然后观察创面变化、感染、全身反应、创面愈合等情况,并进行比较和统计学分析.结果总有效率治疗组为93.33%,对照组为91.11%,两组比较差异无显著性(P >0.05).结论凉血解毒油治疗Ⅱ°烧伤与京万红油膏比较更能加速创面愈合,减轻临床症状,治疗效果理想.     

雾化烧伤灵药效学研究

目的　观察雾化烧伤灵治疗烧伤的药理学作用并探讨其作用机制。方法　制作豚鼠浅Ⅱ度、深Ⅱ度烧伤模型及Ⅲ度烧伤感染模型 ,观察雾化烧伤灵组 (实验组 )、湿润烧伤膏组 (药物对照组 )及空白对照组 (溶剂组 )烧伤创面结痂面积、脱痂时间和愈合时间的变化。结果　与空白对照组比较 ,雾化烧伤灵组可明显减少创面渗出、缩小结痂面积、缩短脱痂时间及愈合时间 (P <0 .0 1) ,且其作用明显优于湿润烧伤膏组 (P <0 .0 5 ) ,并可抑制烧伤创面绿脓杆菌的感染。结论　雾化烧伤灵具有明显的促进烧伤创面愈合及创面抗感染作用 ,是一种较理想的外用治疗烧伤药物     

改良负压封闭引流技术在Ⅳ期压疮应用中的临床效果

目的:评价改良式负压封闭引流技术在治疗Ⅳ期压疮中的应用效果。方法:选取2009年10月-2012年10月在我院就诊的Ⅳ期压疮患者45例,随机分为实验组(23例)和对照组(22例),实验组采用改良负压封闭引流装置处理伤口,对照组采用传统换药处理伤口,比较两组患者伤口愈合创面感染控制情况、伤口换药平均时间,患者伤口疼痛评价及每例伤口的均次数换药费用。结果:实验组创面的愈合效果、愈合时间和创面感染控制率与对照组比较。差异有统计学意义(P<0.05);实验组患者在疼痛、换药频次与对照组比较,差异有统计学意义(P<0.05)。结论:采用改良负压封闭引流技术治疗Ⅳ期压疮可明显缩短创面愈合时间、减轻患者痛苦、降低治疗费用。     

湿性敷料治疗压疮的有效性研究

目的 探讨湿性敷料治疗压疮的有效性.方法 将Ⅰ～Ⅳ期压疮患者43例(53处),随机分为实验组22例(28处)和对照组21例(25处),实验组使用湿性敷料换药治疗创面,对照组使用常规换药方法治疗创面.比较两组患者创面的愈合情况、愈合时间、感染例数.结果 两组患者治疗后创面愈合情况、感染例数、Ⅲ期压疮愈合时间及换药次数比较差异均有统计学意义(P<0.05).结果 湿性敷料治疗压疮疗效确切,治愈率达60.7%,感染率降低至3.6%,还可减少换药次数,缩短愈合时间,适合临床推广.     

马勃联合鱼肝油滴剂治疗压疮的疗效观察及护理体会

目的 探讨马勃联合鱼肝油滴剂治疗压疮的临床疗效及护理方法.方法 选取2013年8月-2015年8月我院80例压疮患者,随机分为对照组和观察组,对照组采用传统护理方法治疗,观察组采用马勃联合鱼肝油滴剂治疗,比较2组的临床疗效.结果 2组临床效果比较,观察组治疗有效率为92.5％,对照组治疗有效率为72.5％,差异具有统计学意义(P＜0.05);2组愈合时间比较,观察组为(7.46±2.37)d,对照组为(12.32±2.12)d,差异显著具有统计学意义(P＜0.05).结论 马勃联合鱼肝油滴剂治疗压疮效果良好,能加快创面愈合,减轻患者痛苦,值得临床推广.     

机械磨削联合微针导入重组人酸性成纤维细胞生长因子和基因重组人表皮生长因子促进创面愈合的临床观察

目的探讨机械磨削联合微针导入重组人酸性成纤维细胞生长因子(rhaFGF)和基因重组人表皮生长因子(rhEGF)促进创面愈合的临床效果。方法 2010年4月—2011年9月表浅瘢痕患者60例,随机分为试验组、对照组和空白对照组,每组20例。试验组采用机械磨削联合微针导入rhaFGF和rhEGF,对照组采用机械磨削联合外涂rhaFGF及rhEGF,空白对照组采用机械磨削。研究三组创面愈合时间。结果试验组平均创面愈合时间(9.35±0.87)d,对照组(10.02±0.96)d,空白对照组(10.66±1.02)d,三组比较差异有统计学意义(P<0.05);其中试验组平均创面愈合时间较对照组和空白对照组均明显缩短,差异有统计学意义(P<0.05);对照组平均创面愈合时间较空白对照组明显缩短,差异有统计学意义(P<0.05)。结论机械磨削联合微针导入rhaFGF和rhEGF可促进创面愈合,缩短了创面愈合时间,提高了愈合质量。     

复方薰衣草油膏治疗Ⅱ度烧伤创面临床对比观察

目的:观察复方薰衣草油膏对于Ⅱ度烧伤创面的治疗效果。方法:随机选取烧伤后早期入院的中小面积以Ⅱ度烧伤为主的成年患者,按照同体对照分别采取两种不同治疗方法:实验组创面外用复方薰衣草油膏治疗,对照组创面仅外用SD-Ag糊剂治疗。对比观察两组创面愈合时间、创面感染率、愈后瘢痕增生等指标。结果:外用复方薰衣草油膏治疗组比单纯外用SD-Ag糊剂组可明显缓解疼痛、改善创面且愈合时间短、愈后增生性瘢痕轻、创面感染率低。结论:Ⅱ度烧伤创面早期应用复方薰衣草油膏能起到缓解疼痛、避免创面加深、缩短病程、减轻炎症反应的作用,并且能减轻愈后疤痕。     

裂口扩创加内括约肌切开术联合中药治疗慢性肛裂

目的 观察裂口原位扩创加内括约肌切开术联合中药治疗慢性肛裂的临床疗效.方法 将94例慢性肛裂患者随机分为治疗组和对照组,各47例,治疗组采用裂口扩创加内括约肌切开术,对照组采用开放性肛门右后侧切术治疗,2组术后均给予痔疾洗液坐浴及红油膏敷创,观察2组术后创面疼痛、水肿、出血的变化,以及创面感染、裂开等不良反应,并评价临床疗效.结果 治疗组术后第3、10天创面水肿、疼痛等症改善情况优于对照组(P＜0.05),创面愈合时间、术后创面不良反应以及术后肛门功能评价方面优于对照组(P＜0.05).结论 裂口扩创加内括约肌切开术联合中药可减轻慢性肛裂术后并发症和肛门功能损害,缩短创面愈合时间.     

矾冰纳米乳对大鼠深Ⅱ度烫伤创面治疗作用的研究

目的 研究矾冰纳米乳对大鼠深Ⅱ度烫伤创面的治疗作用.方法 140只SD大鼠,制成深Ⅱ度烫伤模型,随机分为7组,即空白对照组、基质组、阳性对照组、矾冰纳米乳低、中、高剂量组及矾冰液组.采用相应药物局部涂抹,2次/d,0.2 mL/次,连续给药21 d.每天观察创面愈合情况,记录创面愈合时间,分别于第7、14及21天计算创面愈合率,第7天取创面组织测定皮肤羟脯氨酸含量并进行病理组织学检验.结果 矾冰纳米乳各剂量组、烫疮油、矾冰液组创面于19～25d基本愈合,而空白对照组、基质组创面于23～27 d基本愈合;矾冰纳米乳低剂量、烫疮油组、矾冰液组及中高剂量组与空白对照组比较,能缩短创面愈合时间(P<0.05;P<0.01),创面愈合百分率明显提高(P<0.05;P<0.01);各实验组羟脯氨酸含量在烫伤后7d高于空白对照组,但差异无显著性;创面组织病理形态学结果 提示矾冰纳米乳具有明显的促进烫伤创面愈合的作用.结论 矾冰纳米乳可明显提高大鼠深Ⅱ度烫伤创面愈合率,缩短创面愈合时间,加速创面愈合.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.