4种培养基口腔毛滴虫培养效果的比较

目的:探讨口腔毛滴虫体外培养的理想培养基。方法:配制由牙垢等成分构成的培养基并进行口腔毛滴虫培养。以LES双相培养基作为对照,观察培养效果。在培养过程中,每隔24 h对各种培养基中口腔毛滴虫的繁殖情况、存活率和活动能力等作动态观察,并进行统计学分析。结果:口腔毛滴虫在4种培养基中均在3 d内达到生长高峰,但在培养基Ⅱ中存活时间超过15 d,而培养基Ⅰ(LES双相培养基)、培养基Ⅲ、培养基Ⅳ的存活时间分别为13、11、9 d。此外,口腔毛滴虫在培养基Ⅱ中的虫体密度均大于其他3种培养基。结论:添加牙垢的培养基Ⅱ为培养口腔毛滴虫的最佳培养基,可推广应用。     

人芽囊原虫多样化繁殖方式短暂共存现象

目的为了解人芽囊原虫对外界条件的适应性,观察培养条件改变对其形态和繁殖方式的影响. 方法从腹泻患者粪便中检查并分离培养获得人芽囊原虫,分别用locke氏-鸡蛋双相培养基（LES）和改良1640培养基（RPMI-1640）培养,改变培养条件,观察人芽囊原虫形态和繁殖方式. 结果在两种培养基中均可见到人芽囊原虫的四种主要形态：空泡型、颗粒型、阿米巴型和包囊型,其繁殖方式以二分裂为主,可见少量出芽生殖.当从LES培养基突然变为RPMI-1640培养基时,人芽囊原虫出现短时间（48～72h）内生长停滞,随后出现多种形式的繁殖方式,可见较多的二分裂、出芽生殖、三分裂、多分裂、裂体生殖,持续24h后恢复为二分裂为主. 结论培养条件改变能够引起人芽囊原虫繁殖方式的改变,出现短暂性多样化繁殖方式,人芽囊原虫对环境的适应能力很强.     

人表皮细胞在三种无血清培养基中的生长与增殖

对人原代表皮细胞在无血清培养基 A、B和 C中的生长过程进行动态观察 ,并于培养第 5 d用 MTT法和3H- Td R掺入法分别测定其生长和代谢情况。结果表明 :人原代表皮细胞在无血清培养基 A中生长 (OD值 :0 .6 38± 0 .0 33)和代谢 (COM:92 8± 46 .6 9)优于无血清培养基 B(OD值 :0 .5± 0 .0 37,CPM:740 .11± 43.2 9) ,差异有极显著意义 (MTT法 :P<0 .0 1,3H - Td R掺入法 :P<0 .0 1)。在无血清培养基 B中人原代表皮细胞在培养第 12 d仍未汇合联接成片 ;而在无血清培养基 C中细胞的生长增殖与无血清培养基 A相似 (P>0 .0 5 ) ,细胞在这二种培养基中于培养第 12 d均可汇合联接成片。     

兔角膜基质细胞的体外培养及生物学特性

目的改进兔角膜基质细胞培养技术,观察其生物学特性。方法　先同时消化角膜上皮及内皮层,然后刮去上皮、内皮、前弹力和后弹力层,接着将剩余的基质层剪碎消化、接种,采用MTT法观察细胞生长情况,并对比有、无血清培养基对细胞生长的影响。结果　角膜基质细胞10d后形成细胞单层,增殖旺盛。有血清培养基培养到第8天左右达到生长高峰,无血清培养基培养的细胞生长较慢,第9天左右达到生长高峰。结论　改进的角膜细胞培养技术简便、成功率高,培养出的角膜基质细胞生长良好。血清对角膜基质细胞生长有一定的影响。     

兔角膜基质细胞的体外培养及生物学特性

目的 改进兔角膜基质细胞培养技术，观察其生物学特性。方法 先同时消化角膜上皮及内皮层，然后刮去上皮，内皮，前弹力和后弹力层，接着将剩余的基质层剪碎消化，接种，采用MTT法观察细胞生长情况，并对比有，无血清培养基对细胞生长的影响。结果 角膜基质细胞10d后形成细胞单层，增殖旺盛，有血清培养基培养到第8天左右达到生长高峰，无血清培养基培养的细胞生长较慢，第9天左右达到生长高峰，结论 改进的角膜细胞培养技术简便，成功率高，培养出的角膜基质细胞生长良好，血清对角膜基质细胞生长有一定的影响。     

DMEM培养基体外培养阴道毛滴虫效果的实验观察

目的研究阴道毛滴虫在不同浓度胎牛血清DMEM培养基中的生长效果,比较阴道毛滴虫在DMEM培养基与15%肝浸汤培养基中的生长情况。方法调节DMEM培养基中胎牛血清终浓度分别为10%、20%、50%,接种密度为15×104/ml的阴道毛滴虫,置37℃恒温箱培养,每24小时取样一次,光镜观察虫体形态,利用血球计数板计数阴道毛滴虫增殖密度并计算增殖倍数。接种密度为1. 8×104/ml的阴道毛滴虫分别至DMEM、15%肝浸汤培养基中,比较阴道毛滴虫生长时间、增殖倍数及形态。结果接种密度为15×104/ml的阴道毛滴虫在终浓度为10%、20%、50%胎牛血清的DMEM培养基内分别培养48 h、24 h、72 h达增殖高峰。接种密度为1. 8×104/ml的阴道毛滴虫在DMEM培养基中120 h增殖倍数达高峰,为32. 63倍,虫体形态大而圆;在15%肝浸汤培养基中96 h增殖倍数达高峰,为59. 40倍,虫体形态多呈梨形;虫体在DMEM培养、肝浸汤培养基中生存时间最长分别为14 d、5 d。结论阴道毛滴虫在DMEM培养基中增殖率低于在肝浸汤培养基中,但虫体在DMEM培养基中存活时间更长,其形态大而圆,因此DMEM培养基适合阴道毛滴虫的体外生长,保种,染色,及形态观察。     

雨生红球藻fachb-712快速繁殖生长的适宜条件

目的 探讨雨生红球藻fachb-712快速繁殖生长的适宜条件.方法 利用分光光度法和显微镜计数法绘制光密度与细胞密度的关系曲线,从不同的pH值、光照强度、接种密度、温度等培养条件入手,对影响雨生红球藻生长的各种主要培养条件进行初步研究.结果 雨生红球藻生长的适宜培养条件为:pH 8.0;光照强度1 klx下连续培养24h;温度24 ℃;接种量1.95×105mL-1.结论 雨生红球藻细胞密度与培养物光密度值满足线性关系,pH 8.0、光照强度1 klx下连续培养24h、温度24 ℃、接种量1.95×105mL-1的培养条件适宜雨生红球藻快速繁殖生长.     

人芽囊原虫在RPMI1640培养基中培养条件的优化

目的优化人芽囊原虫(Blastocystishominis)在RPMI1640培养基中的培养条件。方法将B.hominis接种至RPMI1640培养基中,观察酸碱度、接种量、血清种类、浓度、温度、空气中氧与粪便标本的选择对B.hominis生长的影响。结果在RPMI1640培养基中,pH7.5、接种量200000细胞/管、20%小牛血清,37℃,加入青霉素、链霉素为最佳培养条件。结论在RPMI1640中,接种以B.hominis阳性(颗粒型为主)的血粘液便,加入20%小牛血清,青霉素、链霉素,在pH7.5于37℃条件下厌氧培养,每6d转种一次,可达到长期培养B.hominis的目的。     

胎鼠牙胚细胞体外培养研究

目的 摸索正常胎鼠牙胚细胞离体培养所需条件，为进一步将其作为牙组织工程种子细胞奠定基础。方法 体视显微镜下取17d龄胎鼠下颌第一磨牙牙胚，胶原酶／分离酶消化，以含10％胎牛血清(FCS)的DMEM为培养基进行原代培养。倒置显微镜下观察培养细胞的生长特点，MTT法测定细胞活性绘制生长曲线，取原代培养4～9d的细胞进行组织化学和免疫组织化学染色。结果 倒置显微镜下观察，培养细胞在24h内贴壁，约7～9d生长连成片状。细胞有多角形、大而扁平梭形和长梭形几种形态。生长曲线表明：培养细胞在前3天生长缓慢，第4天呈对数生长，第7天达高峰。免疫组化结果表明：培养细胞来源于两种组织。结论 采用酶消化培养法，用胶原做基质饲养层，含10％FCS的低糖DMEM作为培养基，能将胎鼠牙胚细胞培养成活，并维持其细胞学特征。     

迈氏唇鞭毛虫的培养观察

目的 :研究迈氏唇鞭毛虫在体外培养的生长情况。方法 :采用 L ocke- Egg- Serum(L ES)培养基 ,血清浓度为 2 5 % ,PH5 .8和 6 .8,无菌条件下 37℃培养。结果 :滋养体能在 L ES培养基中生长 ,接种第 2天后 ,生长量达最大。转种培养时 ,生长情况没有初始培养生长旺盛。结论 :迈氏唇鞭毛虫的体外培养 ,在目前情况下 ,初始培养能获取更多的虫体。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.