反相高效液相色谱法测定茶多酚中表没食子儿茶素没食子酸酯的含量

目的采用反相高效液相色谱法测定茶多酚中表没食子儿茶素没食子酸酯(EGCG)的含量。方法以甲醇∶水∶乙酸=26∶73.5∶0.5(体积比)为流动相(1.0mL/min),C18柱(250.0mm×4.6mm,5μm),UV检测器(278nm)。结果EGCG在0.1303￣0.6517mg/mL范围内线性关系良好(r=0.9995),平均回收率为100.26%,RSD为0.86%。结论该法简便、快速,可作为测定茶多酚中EGCG含量的方法。     

高效液相色谱法测定鸡骨草胶囊中相思子碱的含量

目的 建立鸡骨草胶囊中相思子碱的含量测定方法.方法 采用反相高效液相色谱法测定鸡骨草胶囊中相思子碱的含量.色谱柱为Phenomenex-ODS(250 mm×4.0 mm,5 μm)柱;流动相为甲醇-0.5%(体积分数)冰乙酸溶液,梯度洗脱;流速为1.0 mL/min;检测波长为270 nm;温度为室温.结果 相思子碱...     

高效液相色谱法测定丙戊酸钠中醋酸的含量

目的 建立使用高效液相色谱法(HPLC)测定丙戊酸钠中醋酸含量的方法.方法 色谱柱为Nano ChromCoreC8(4.6 mmX150 mm,5 μm);梯度洗脱,流动相A为磷酸水溶液(磷酸调节pH值至2.3),流动相B为甲醇;检测波长为210 nm;流速为0.5 mL/min;柱温为40 ℃;进样体积10 μL....     

反相高效液相色谱法测定野生四叶参中氨基酸

目的:建立测定野生四叶参中氨基酸含量的反相高效液相色谱法(RP-HPLC).方法:用盐酸水解大别山区野生四叶参中的蛋白质.采用反相高效液相色谱法,色谱条件为:色谱柱:Hypersil ODS-BP C18柱(4.6 mm×250 mm,5 μm);流动相A:醋酸-醋酸钠缓冲液(pH=7.2);流动相B:甲醇,流速1.0 mL/min,流动相采用梯度洗脱方式,荧光检测波长为450 nm.分析四叶参中各种氨基酸含量.采用外标法定量.结果:氨基酸在25.0～800.0 μmol/L范围内浓度与色谱峰面积有良好的线性关系(r≥0.993),该方法的回收率在89.5%～109.2%,RSD在3.2%～9.0%,检测限在1.0～3.7 μmol/L.野生四叶参中含有12种氨基酸,总氨基酸含量为51.49 mg/g,必需氨基酸含量为6.95 mg/g.结论:建立的分析方法精密度好、准确度高,适用于野生四叶参中氨基酸含量的分析.     

养心草中槲皮苷的含量测定

目的建立养心草中槲皮苷含量的测定方法。方法用反相高效液相色谱法,色谱柱:AQ-C18(4.6 mm×250 mm,5μm);流动相:甲醇-乙腈-0.1%磷酸溶液梯度洗脱;检测波长:254nm;流速:0.8 ml/min;柱温:30℃。结果槲皮苷在8.16～816μg/mL有良好的线性关系。结论反相高效液相色谱法操作简便,检测灵敏,有良好的重复性和稳定性,可测定养心草中槲皮苷的含量。     

水牛角与羚羊角的HPLC法鉴别

建立一种鉴别水牛角与羚羊角药材的质控方法。采用OPA柱前衍生反相高效液相色谱法,色谱柱为HP Hypersil ODS柱(125 mm×4.6mm×5μm),以十八烷基硅烷键合硅胶为填充剂。流动相A:8.0 mmol/L磷酸盐缓冲液(pH7.2),含φ=0.3％四氢呋喃;流动相B:8.0 mmol/L磷酸盐缓冲液(pH7.2)+甲醇+乙腈(50+35+15)。线性梯度洗脱;荧光检测器,Ex=340nm,Em=450 nm。结果水牛角与羚羊角药材在氨基酸的含量上有明显差异。本文所建立的方法简便,重现性好,可用于水牛角与羚羊角药材的鉴别。     

HPLC法测定广东土牛膝中腺苷的含量

目的 测定广东土牛膝中腺苷的含量.方法 采用反相高效液相色谱法,色谱柱:DiamonsilTM C18柱,流动相:甲醇-磷酸盐缓冲溶液(体积比12:88),流速:1. 0 mL·min-1 ,检测波长260 nm,柱温30 ℃.结果 腺苷的平均回收率为99.5%,RSD值为1.69%;方法 精密度试验RSD值为1.15%(n=6).结论 该法可用于广东土牛膝中腺苷的含量测定.     

HPLC法测定板蓝根药材及农本方颗粒中表告依春的含量

目的:采用HPLC法比较板蓝根药材及其农本方颗粒中表告依春的含量。方法:采用反相高效液相色谱法,色谱柱为Kromail C18(250mm×4.6mm,5μm);以乙腈-水(15∶85)为流动相,流速:1.0 mL/min;检测波长245 nm,柱温30℃。结果:表告依春在6.3~84.0μg/mL范围内浓度与峰面积成良好的线性关系,r=0.9995;板蓝根粉末平均回收率为99.13%,RSD为1.21%;农本方颗粒平均回收率为99.09%,RSD为1.31%;测得板蓝根粉末以及农本方颗粒中表告依春含量分别为4.81mg/g,4.53mg/g。结论:本实验建立的高效液相色谱法准确、可靠,可用于板蓝根药材及其制剂的质量评价。结果表明板蓝根农本方制剂中的表告依春含量达到其药材中含量的90%以上。农本方颗粒制剂值得推广。     

高效液相色谱法测定奶茶中表儿茶素和咖啡因的含量

目的:对6种不同品牌的奶茶中表儿茶素和咖啡因的含量进行测定比较,为奶茶的质量评价提供可靠依据.方法:采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水,梯度洗脱,流速1.0 ml/min,检测波长为254 nm,柱温40℃.结果:在上述色谱条件下检测,6种奶茶样品中均含有咖啡因,...     

柱前衍生高效液相色谱法测定瓜蒌皮中游离氨基酸含量

目的:建立柱前衍生高效液相色谱法(RP-HPLC)测定瓜蒌皮中游离氨基酸含量的方法。方法 :采用异硫氰酸苯酯(PITC)为柱前衍生试剂,高效液相色谱法色谱柱为(Atlantis dC18 Column(3μm,4.6 mm×150 mm,Waters),流动相A:甲醇-乙腈(1∶2),流动相B:0.15 mol/L乙酸钠,流速1.0mL/min,梯度洗脱,柱温35℃,吸收波长254 nm。结果:各种氨基酸均能达到基线分离。结论:柱前衍生高效液相色谱法作为瓜蒌皮药材中游离氨基酸的测定手段之一,准确可靠。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.