桔梗试管苗茎尖玻璃化法超低温保存及植株再生

目的为桔梗Platycodongrandiflorum种质资源的保存提供一条新途径。方法采用玻璃化法研究了桔梗试管苗茎尖超低温保存及植株再生。结果桔梗嫩梢在培养基(MS 5%DMSO 103g/L蔗糖)上培养3d,切取2~3mm茎尖,室温(20℃)下装载液(60%PVS2)过渡20min,0℃下玻璃化液(PVS2)处理90min,投入液氮保存1d后,40℃水浴化冻,含410g/L蔗糖的MS培养基洗涤20min,接种于含0.6mg/LKT、0.2mg/LBA、0.05mg/LNAA的MS培养基表面的滤纸上,暗处理1d后转移到新鲜的上述再生培养基中,暗培养1周后转到正常光下,80%以上成活,植株生长正常。结论桔梗种质资源的玻璃化法超低温保存操作简单、成活率高、再生植株正常,可用于生产实践。     

佛手茎尖玻璃化超低温保存和植株再生

目的 为佛手种质资源的保存提供一条新途径。 方法 对佛手茎尖进行了玻璃化超低温保存,并对保存后的茎尖进行微嫁接试验,获得再生植株。 结果 佛手茎尖在含5%DMSO和5%蔗糖的MS培养基上预培养48 h后,切取3～4 mm的茎尖,室温(25 ℃)下装载液(60%PVS2)预处理30 min,0 ℃下玻璃化液PVS2处理80 min,投入液氮保存24 h后取出,在40 ℃水浴快速化冻,室温下分别用1.2 mol/L蔗糖和MT基本培养基洗涤2次,每次10 min。保存后的茎尖经TTC法检测,成活率达81.25%;将茎尖嫁接到黑暗培养基的砧木上,再生率可达52.94%,且再生植株生长和分化正常,经PCR检测不含黄龙病病原。 结论 玻璃化超低温保存方法可用于佛手种质资源保存。     

罗汉果试管苗茎尖玻璃化法超低温保存及植株再生

目的 为罗汉果Siraitia grosvenorii种质资源的保存提供一条新途径。方法 以继代15 d生长健壮的罗汉果试管苗0.8～1 cm长嫩梢进行预培养,剥取2～3 mm茎尖,25 ℃下60% PVS₂装载,再用100% PVS₂在0 ℃脱水处理,更换新鲜100% PVS₂后投入液氮保存24 h,化冻,用MS+1.2 mol/L蔗糖的液体培养基洗涤,滤纸吸干后接种于MS+1.0 mg/L 6-BA+0.05 mg/L NAA+0.1 mg/L GA₃+0.8%琼脂粉+45 g/L蔗糖的恢复培养基上, 25 ℃暗培养7 d, 转入正常光下培养。再生苗生根培养基为1/2 MS+0.2 mg/L NAA+30 g/L蔗糖。结果 嫩梢于MS+0.7 mol/L蔗糖的培养基上预培养3 d,茎尖装载40 min,脱水50 min,液氮保存后于40 ℃水浴中快速化冻,洗涤40 min,恢复培养1 周时存活率最高可达100%,30 d时再生率最高可达78.33%。再生苗转入生根培养基可形成完整植株。结论 罗汉果种质资源的玻璃化法超低温保存操作简单、成活率高、再生植株正常。     

茅苍术种质资源的超低温保存

目的:为茅苍术种质资源的保存提供一条新途径。方法:采用干冻法研究了茅苍术种子超低温保存,采用玻璃化法研究了试管苗茎尖的超低温保存。结果:采用干冻法冻存的茅苍术种子发芽率在70%以上,采用玻璃化法冻存的茅苍术试管苗茎尖,再生率在30%以上。结论:超低温保存可用于茅苍术种子及试管苗茎尖的保存,为茅苍术种质资源的长期保存提供了理论依据。     

黄独带芽茎段增殖和生根的初步研究

目的对黄独带芽茎段的增殖和生根进行初步的研究。方法采用单因子试验和植物组织培养的方法。结果6-BA或KT与NAA组合有利于黄独带芽茎段增殖;蔗糖质量浓度过高将导致黄独带芽茎段愈伤化不利于黄独带芽茎段增殖;液体培养有利于黄独带芽茎段增殖;在一定范围内NAA质量浓度的增加有利于黄独带芽茎段生根,但质量浓度太高将会抑制生根。结论黄独带芽茎段增殖的最佳培养基为MS KT 2 mg/L NAA 1 mg/L或MS 6-BA 2 mg/L NAA 0.5 mg/L;黄独带芽茎段再生的最佳蔗糖量为30 g/L,最佳培养方式为液体培养,最佳的生根培养基为MS NAA 2 mg/L。     

獐牙菜的组织培养

目的 针对獐牙菜野生资源受到严重破坏的情况,系统地探讨了通过组织培养为手段进行人工繁殖的方法。方法 将种子接种于诱导培养基上,待其长成小苗后分别取其不带芽茎段、叶和带芽茎段作为外植体,在MS培养基上添加不同的激素配比,改变培养方式。结果 在所有的实验方案中,不带芽茎段是理想的外植体材料。较适宜的初代培养基为MS+BA 0.5 mg/L+蔗糖3.0%,增殖培养基为MS+BA 0.5 mg/L+IBA 0.1 mg/L+蔗糖3.0%,而根的诱导则是在1/2MS+NAA 0.5 mg/L+1.5%蔗糖的培养基上进行。结论 采用组织培养方式可进行獐牙菜的快速繁殖,为确保这一珍稀药用植物资源的保护和可持续利用提供有效途径。     

甘肃道地黄芪种苗的离体快繁研究

目的筛选出适于无菌短枝快繁的培养基及研究试管苗移栽驯化技术。方法以MS为基本培养基添加不同质量浓度的BA和NAA,对黄芪的不同外植体进行组织快繁研究。结果一年生植株的带芽茎段在MS BA0.1mg/L培养基上短枝长势最好;一年生植株的新生芽和两年生植株的带芽茎段,在MS BA0.8mg/L NAA0.1mg/L培养基上均能产生丛生芽。将短枝接种于1/2MS 0.1%活性炭或1/2MS NAA1.5mg/L 0.1%活性炭的培养基上,经诱导产生有效根。小苗经沙培驯化后,移栽成活。结论利用蒙古黄芪的无菌短枝快繁优良种苗是一种经济、快速、可行的育苗方式。     

凤丹的体外增殖研究

为探索凤丹体外增殖的最佳条件,利用组织培养技术进行观察。结果显示以下培养条件较适宜：凤丹种子灭种后在35℃下于遥床上振动72h,取出胚,在1/2MS 10%椰子汁的培养基上培养1个月,然后将带有腋芽的茎的茎段切下置于MS BA（1mg/L)培养基上进行培养,形成丛生芽,当芽长出2-3片叶子时,转移至MS IBA（1mg/L) 3%蔗糖培养基上,1个月后即长出白色的直根,形成完整的植株。     

罗汉果脱毒苗的快速繁殖研究

目的筛选适宜的罗汉果脱毒苗茎段增殖培养基,研究培养温度与光照对茎段增殖培养的影响。方法采用正交试验设计和完全随机实验设计筛选罗汉果脱毒苗茎段增殖培养基。正交L9(34)实验以在M S BA 0.1 m g/L NAA 0.1 m g/L上培养30 d的罗汉果脱毒苗单芽姊妹系的单节茎段为外植体,以培养基中BA,NAA,IBA及蔗糖为试验因子,各因子设3个水平,以在不同处理得到的增殖系数为测定指标;在此基础上进一步研究了培养基中BA和蔗糖质量浓度对增殖效果的影响;采用完全随机实验设计研究温度,光照对茎段增殖培养生成试管苗的影响。结果通过对正交实验结果的极差分析及方差分析表明,在4个因子中,对罗汉果茎段增殖系数影响最大的是BA,其次是蔗糖的NAA,IBA的影响最小;BA,NAA,IBA及蔗糖对增殖系数的影响都达到极显著。根据新复极差检验的结果,当前研究得到的罗汉果离体茎段的最佳增殖培养基为M S BA 0.5 m g/L NAA 0.01 m g/L IBA 0.1 m g/L 蔗糖40 g/L,罗汉果茎段在此培养基上培养30 d后增殖系数为13.13;不同浓度BA与蔗糖对茎段培养的研究结果表明,BA在1.0 m g/L增殖系数最大。考虑为保持试管苗的遗传稳定性,BA为0.5 m g/L为宜。增殖培养基中的蔗糖适宜质量浓度为4.0%;罗汉果茎段培养适宜条件为培养温度25℃,培养光强1 000~3 000lx。结论增殖培养基的成功筛选及培养温度与光强的选择,有助于实现罗汉果脱毒苗的工厂化生产。     

黄独脱毒苗快繁技术的研究

目的 以黄独脱毒苗为试材，研究不同因素对黄独带芽茎段芽增殖和生根的影响，以期对黄独脱毒苗的快繁技术进行优化。方法 采用植物组织培养的方法进行茎尖培养和快繁研究，采用 RT-PCR 法对茎尖脱毒植株进行病毒检测。结果 黄独脱毒苗带芽茎段的最佳培养基是 MS+KT 2 mg/L+6BA 1 mg/L+NAA 0.5 mg/L；黄独脱毒苗带芽茎段增殖的最佳蔗糖质量浓度和琼脂质量浓度分别是 30 和0 g/L；黄独脱毒苗带芽茎段生根的最佳培养基是 1/2 MS+IBA 0.1 mg/L+NAA 0.5 mg/L+PP₃₃₃ 1 mg/L；黄独试管苗移栽的最好基质是珍珠岩∶蛭石 (2∶1)；黄独试管苗移栽时最佳的PP₃₃₃ 质量浓度是 50 mg/L。结论 首次成功建立了黄独脱毒苗的快繁技术，为黄独脱毒苗的工厂化生产奠定了技术基础。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.     

Possible Spread of adenovirus type 3 from poultry to humans ：indirect evidence from an outbreak in China

Objective:To explore the epidemiology and etiology for an outbreak of acute respiratory tract infection that occurred in one county of Jiangsu Province, China 2004. Methods: Only cases meeting the case definition were included in the study. We reviewed the medical records of the cases who were admitted to the local hospitals, interviewed cases by a standard questionnaire, and then described the epidemiotogic features and analyzed risk factors by means of a case-control study. We collected pharyngeal swab specimens and sent them to different laboratories for isolation and culture. The laboratory used different detection methods such as DIP, PCR, electron microscope examination and microneutralization assay, to identify and then type the positive specimens. Results:A total of 871 cases were reported during the period from April 18 to July 4,2004. The distribution of onset times presented two peaks, one in late May and another in middle June. The epidemic occurred mainly in the elementary and junior high schools in ten townships of one county, and the mean age of the cases was 12 years (range 7 months to 18 years). The course of the disease was acute, and was characterized by fever accompanied with sore throat and tonsillitis. The WBC count of cases was normal or elevated. The mean duration of illness was 5 days (range 2 to 12 days). No fatalities from illness were reported. A case-control study indicated that the possible risk factors were close contact with a case and/or poultry before onset and sharing of towels among members of the family. The typical CPE was observed through inoculating pharyngeal swab specimens into the HEP-2 cell cultures in different laboratories. An infection of adenovirus type 3 was verified by detecting positive specimens in different methods. Conclusion:This investigation demonstrated that the acute respiratory infection in cases was caused by adenovirus type 3. Cases occurred in over 70 schools in ten townships in 2004, and the route of transmission was possibly close contact with cases or droplet transmission.