8-羟基喹啉修饰电极阳极溶出伏安法测定人体中痕量锌

本文用强吸附法研制了8-羟基喹啉化学修饰电极,并将其用于人体中痕量锌的分析。由于修饰电极上的8-羟基喹啉能与锌生成疏水性螯合物,从而大大提高了电极的选择性富集效率,使此法具有较高的灵敏度和较好的选择性。在测定血液、头发、指甲等人体样品时,一般不需加镓克服电极上铜锌互化物的生成,就可直接测定锌,方法检出下限为7.6×10~(10mol)/L(受空白所限)。实测人体样品,锌的回收率为93.4±5.7％(X±SD),变异系数CV=6.1％。     

8-羟基喹啉化学修饰电极溶出伏安法测定人体中痕量铜

我们研制了8-羟基喹啉化学修饰电极,并将其应用于人体中痕量铜的分析。由于修饰在电极上的8-羟基喹啉能与铜在一定条件下生成疏水性螯合物,因而能有效地提高电极的选择性畜集效率,使本法具有较高的灵敏度和较好的选择性,方法的检出下限为5×10~(-10)mol/L(受空白所限)。实测人体样品,铜的回收率为94.2±6.0％(±SD),变异系数CV=6.4％。用本法测定了铅蓄电池制造工和对照组的血铜、发铜,获得满意结果。     

高压消化-金电极阳极溶出伏安法测定人体中痕量硒

我们用高压溶样器做人体样品预处理,有效防止了样品消化过程中硒的挥发损失;用金电极阳极溶出伏安技术研究了人体中痕量硒的测定方法,确定了最佳测试条件,检出下限为2×10~(-8)g/L。实测人体样品,硒的回收率为96.6±6.7％( SD),变异系数CV为7.0％。     

邻苯二胺同位修饰电极溶出伏安法测定人发中痕量硒

由于邻苯二胺能与硒形成配位化合物,并且能较好地吸附玻碳汞膜电极表面,因而能有效地提高电极对硒选择性富集。本文用同位修饰电极,以邻苯二胺为修饰剂,测定人发中痕置硒,其检出下限为3.2×10~(-9)mol/L。实测人体样品硒的回收率为97.0±4.3％((?)±s),变异系数CV=4.9％。     

螯合树脂化学修饰碳糊电极的研制及其在发铜测定中的应用

我们研制了螯合树脂(DUOLITE ES 467)化学修饰碳糊电极,并用于人体发样中痕量铜的测定。由于修饰在电极上的螯合树脂与铜离子在进行离子交换的同时发生螯合反应,故本法具有较好的选择性和较高的灵敏度。方法的检出下限为6×10~(-9)mol/L。实测人体样品铜的回收率为108.2±8.3％((?)±s),变异系数=7.7％。     

微分电位溶出分析法在尿铅测定中的应用

1 资料与方法 1.1 试验对象 剑河县1995～1998年驾驶职业性体检人员 1.2 仪器 MP～1型溶出分析仪,MP～1型极普工作台,均为山东电讯七厂产品。 1.3 试剂 1:1硝酸(优级纯);1:1乙醇(清洗电极时使用);1mol硝酸钾溶液;镀汞液:用硝酸汞配成含汞40mg/L硝酸溶液;铅标准溶液:用优级纯硝酸铅配成1ml=10mg铅的1％(V/V)硝酸     

铅中毒诊断标准及铅卫生标准对铅接触者健康保护意义的探讨

目的：探讨铅中毒诊断标难及铅卫生标难对铅接触者健康保护效果。方法：测定某铅冶炼厂158名铅接触工人的血铅(PbB)、尿铅(PbU)、锌gLDb(ZPP)、游离原卟啉(FEP)、δ—氨基乙配丙酸(δ—ALA)含量及车间空气中铅浓度并计算血铅等五项指标超过诊断值的超标率。结果：工人在超过国家卫生标难(铅烟：0．03mg／m^3)726倍、180倍及ll倍的环境中工作，其各项指标的超标率：726倍的为29．6％～57．4％，180倍的为10．0％～48．0％，ll倍的为0～3．7％。结论：现行铅中毒诊断标难及铅卫生标难是安全的，对接触者的身体健康有较好的保护效果。     

碳糊电极的研制及其在食品铅测定中的应用

本文研究了碳糊电极的研制 ,并对其性能进行了测试 ,并且用于食品铅的测定中。在 10 .0mg/LHg2 +- 0 .2 5mole/L盐酸溶液中测量铅 ,检测限达到 0 .0 2 0 μg/L(电富集 10分钟 ) ,用于测定食品中的铅含量。标准加入回收率为 93 .5 %。RSD(n =11) <8.3 %。碳糊电极的峰高均值为 2 68.5 7μA/V ,标准差为 3 .2 5 6μA/V ,变异系数为 1.2 13 %。GC电极的峰高均值为 3 73 .3 μA/V ,标准差为 5 .7987μA/V ,变异系数为 1.5 5 3 %。碳糊电极具有比GC电极更高的稳定性。溶出分析的电极以GC玻碳电极最灵敏 ,但对于碳糊电极的研究很少 ,碳糊…     

儿童血铅含量相关因素的研究

众所周知,铅是一种有毒的渗透性化学物质,可导致儿童在神经系统、生理系统和行为方面出现问题。血铅浓度较低时,可造成人听力障碍和智商降低,血铅浓度较高时,可出现严重脑部疾病、记忆力丧失甚至死亡。因此,即使是暴露在相对较低的铅环境中,铅对人体也会产生危害。目前,世界卫生组织(WHO)和美国疾病预防控制中心(CDC)将血铅浓度≥10μg/dL作为人体血液铅含量超标的参     

微分溶出伏安法测定中成药中的微量砷

在中药的出口中 ,对中药砷、铅、汞的含量控制非常严格 ,因此 ,中草药、中成药中砷、铅、汞含量的测定非常重要。本文采用微分溶出分析法[1] 测定中成药中微量砷 ,较原子吸收光谱法 ,具有操作简便、仪器价格低廉、快速。1　材料与方法1.1　仪器　MP - 2型溶出分析仪 (山东电讯七厂 )三电极系统 :玻碳电极、铂电极、饱和甘汞电极。1.2　试剂1.2 .1　镀汞液　称取硝酸汞 68.5mg、硝酸钾 2 5 .3g混合溶于纯水中加入 0 .63ml浓硝酸 ,用蒸馏水定容 10 0 0ml。1.2 .2　砷标准溶液　取 10ml标准储备液 ( 10 0 μg/ml)与 10 0ml容量瓶中 ,加 1%硝酸…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.