系数倍率法测定氧氟沙星地塞米松滴眼液的含量

采用系数倍率法,在波长λ１＝２４３．５ｎｍ、λ２＝２９４ｎｍ处测定氧氟沙星地塞米松滴眼液的紫外吸收度的差值ΔＡ,以ΔＡ对药物浓度进行线性回归,得氧氟沙星的线性回归方程：Ｃ＝－２０．３５ΔＡ－０．０７９,相关系数ｒ＝０．９９９７,地塞米松的回归方程为：Ｃ＝－１．７４ΔＡ－０．００７,相关系数ｒ＝０．９９９７,氧氟沙星平均回收率为１０１．１％,ＲＳＤ为１．４０％,地塞米松的平均回收率为９８．５％,ＲＳＤ为０．９７％。     

系数倍率法测定氧氟沙星地塞米松滴眼液的含量

采用系数倍率法,在波长λ１＝２４３．５ｎｍ,λ２＝２９４ｎｍ处测定氧氟沙星地塞米松滴眼液的紫外吸收度的差值△Ａ,以△Ａ对药物浓度进行线性回归,得氧氟沙星的线性回归方程：Ｃ＝－２０．３５△Ａ－０．０７９,相关系数ｒ＝０．９９９７,地塞米松的回归方程为：Ｃ＝－１．７４△Ａ－０．００７,相关系数ｒ＝０．９９９７,氧氟沙星平均回收率为１０１．１％,ＲＳＤ为１．４０％,地塞米松的平均回收率为９８．５％,ＲＳ     

HPLC法测定长效异乐定胶囊中5—单硝酸异山梨酯的含量

用反相高效液相色谱法测定了长效异乐定胶囊中５－单硝酸异山梨酯的含量。色谱柱为ＹＷＧ－ＯＤＳ Ｃ１８（２５０ｍｍ×４．６ｍｍＩ．Ｄ，５μｍ）；流动相为甲醇－水（１：１）；检测波长为２３０ｎｍ。本法测定平均回收率为９９．２９％，ＲＳＤ＝０．２４％；日内精密度ＲＳＤ为０．３２％；日间精密度ＲＳＤ为０．９２％。与比色法比较，本法测定结果准确可靠。     

薄层扫描法测定健脾口服液中百秋李醇的含量

目的：采用薄层扫描法对健脾口服液中百秋李醇进行含量测定。方法：样品经过适当提取后点于硅胶Ｇ薄层板上,以石油醚（３０～６０℃）－乙酸乙酯－冰醋酸（９０：１５：０．２）为展开剂,５％三氯化铁乙醇液显色,检测波长λＳ＝５９０ｎｍ,λＲ＝６５０ｎｍ。结果：平均回收率为９９．３１,ＲＳＤ＝１．４０％。结论：薄层扫描法简便、灵敏、准确,可用于健脾口服液质量控制。     

用薄层色谱法测定骨康片中士的宁和马钱子碱含量

目的 建立骨康片中士的宁和马钱子碱的含量测定方法。方法 采用双波长薄层扫描法,硅胶ＧＦ２５４薄层板,展开剂为甲苯－丙酮－乙醇－浓氨试液（４：３：１：２）,检测波长,士的宁λｓ＝２５８ｎｍ,λＲ＝３１０ｎｍ;马钱子碱λＳ＝３０１ｎｍ,λＲ＝３３５ｎｍ。结果 士的宁和马钱子碱的相关系数分别为０．９９９６和０．９９９９,方法的回收率分别为９９．８３％（ＲＳＤ＝１．０３％）和９６．０９％（ＲＳＤ＝２．０１     

亚香棒虫草多糖含量测定

目的　测定亚香棒虫草中多糖的含量。方法　采取硫酸－ 蒽酮比色法于波长为６２４ ｎｍ 测定其含量。结果　亚香棒虫草多糖为１０ ．０ ％ （ ｎ ＝ ５） ,平均加样回收率为１０１ ．２ ％ , Ｒ Ｓ Ｄ＝ １ ．３２ ％ （ ｎ ＝ ５） 。结论　本法操作简便、准确、稳定性好。可为临床替代冬虫夏草提供一理论依据。     

高效薄层扫描法测定四物汤中阿魏酸含量

用高效薄层扫描法（ＨＰＴＬＣ）测定了中药复方四物汤群煎液、分煎混合液、当归和川芎单煎液中阿魏酸的含量。以高效硅胶薄层板，苯－氯仿－乙酸乙酯－甲酸（８∶２∶２∶０．５）为展开剂，在紫外灯３６５ｎｍ波长下定位，用ＣＳ－９３０型薄层扫描仪进行测定，λｓ＝３２０ｎｍ，λＲ＝２４０ｎｍ，狭缝１．２ｍｍ×１．２ｍｍ，线形化参数：ＳＸ＝７，反射法锯齿形扫描，二点外标法定量测定     

双波长紫外分光光度法测定氨茶碱血药浓度

目的：建立氨茶碱的血药浓度测定方法。方法：用双波长紫外分光光度法测定氨茶碱的血药浓度,测定波长为２７４ｎｍ,参比波长为２９８ｎｍ。结果：样品浓度在０．５～２．５μｇ／ｍｌ范围内,与ΔＡ＝Ａ２７４－Ａ２９８呈良好的线性关系。标准曲线：Ｃ（μｇ／ｍｌ）＝１８．１７４４ΔＡ－０．２１８８,ｒ＝０．９９８９。方法的平均回收率为１００．４４％,相对标准偏差ＲＳＤ为３．８０％。结论：双波长紫外分光光度法能消除血清干扰,方法简便、快速、准确。     

高效液相以谱法测定小儿健脾冲剂中橙皮甙的含量

以反相高效液相色谱法测定小儿健脾冲剂中橙皮甙的含量。色谱柱：ＹＷＧ－Ｃ１８,流动相：乙腈－水－甲醇－磷酸（２０：８０：０．５：０．１）,检测波长：２８３ｎｍ。橙皮甙在２．６～２６ｕｇ范围内具有良好的线性关系,ｒ＝０．９９９８,回收率为９７．２％,ＲＳＤ为１．２２％。     

小蔓长春花组织培养物中长春胺的含量测定

采用薄层扫描法测定小蔓长春花组织培养物中长春胺的含量。应用高效硅胶Ｇ薄层板，展开剂为石油醚－氯仿－甲醇（１０：１０：１．２）。在λＳ＝４９０ｎｍ，λＲ＝５５０ｎｍ波长处扫描。长春胺标准品的线性范围为０．５３５￣３．７４５μｇ；平均回收率为９８．６８％，ＲＳＤ＝１．９％（ｎ＝６）。小蔓长春花组织培养物经碱性氯仿和２％硫酸提取后点样，按随行外标两点法进行测定，计算样品的含量。测得在固体和液体培养基Ｂ５     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.