Interleukin 1 receptor antagonist prevents the epileptogenesis in a rat model of febrile seizures:critical role of IL-1 signaling in epileptogenesis

OBJECTIVE To investigate whether the transient augment of cytokine Interleukin 1(IL-1) in hippocampus after febrile seizures initiates molecular cascades to promote epileptogenesis.METHODS Febrile seizures(FS) were induced by exposing SD rat pups(P8-10) to a hyperthermal chamber(44±2)℃ to raise the body temperature with stereotyped behaviors.IL-1 receptor antagonist(IL-1ra) was injected into the hippocampus immediately,24 h,3 d or 7 d after FS.0.3,1,3 and 10 ng/0.4 μl of IL-1 were injected alone in normal rat pups(P8-10).CB1 receptor antagonist SR141716A(ip) was administered 3 d or 7 d after FS induction and IL-1 injection.When the rats were adults,their sensitivity to epilepsy was measured by Maximal electroshock(MES).Expression of CB1 receptors was detected by Western Blotting at postnatal d 15 and 45,respectively.RESULTS IL-1ra administered immediately and 24 h after FS reversed the enhanced susceptibility to MES-induced seizures and upregulated the expression of CB1 receptors.IL-1 alone increased both the susceptibility to seizures and the expression of CB1 receptors till day 15 at all doses,and the dose of 1 ng showed a long-lasting effect(at least for 45 d).CB1 receptor antagonist SR141716A abolished the enhanced susceptibility to seizures.CONCLUSION Our study indicates that augmented levels of IL-1 in the hippocampus after FS might promote epileptogenesis by,at least partly,modulating endocannabinoid signaling.     

油酰乙醇胺对高脂血症大鼠降血脂及肝脏的保护作用

目的观察油酰乙醇胺(OEA)对高脂血症模型大鼠降血脂及肝脏保护作用。方法高脂饮食建立高脂血症大鼠模型,分别观察OEA(10,203,0 mg/kg)对高脂血症大鼠的血清胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、谷丙转氨酶(ALT)、肝重和肝脏系数、肝脏丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)的影响。制作冰冻切片观察大鼠肝脏脂质变性程度。结果与模型组相比,OEA(20,30mg/kg)具有降血脂作用,同时降低血清ALT、肝脏脂质、肝重和肝脏系数、肝脏MDA水平,升高肝脏GSH-Px活力。结论 OEA能降低高脂血症大鼠血脂、抑制肝脏脂肪沉积,并减轻脂质过氧化物对肝脏的损伤。