整合素α4β1及其配体与大鼠肝肿瘤周边肥大细胞募集的关系

目的：D整合素α4β1(VLA-4)主其配体VCAM－1（vascular cell adhesion molecular-1)和FN（fibronectin)与肝肿瘤周边肥大细胞（mast cell,MC)募集的关系。方法：根据肝肿瘤周边肥大细胞数量,将18只雄性Wistar大鼠移植肝肿瘤模型进行分析,8只正常雄性Wistar大鼠作对照,用间接免疫荧光和流式细胞术检测各级一大鼠腹腔肥大细胞整合素VLA－4分子的表达水平,同时用免疫组化研究肿瘤周边肝组织血管内皮细胞和肝窦内皮细胞表面VCAM－1和肿瘤周边FN的表达。结果：不同肝肿瘤大鼠肿瘤周边浸润肥大细胞数量有明显差异。各组大鼠腹腔MC表达整合素VLA－4分子均呈阳性,肿瘤周边肥大细胞浸润较多组,其整合素α4β1表达水平也较高。肿瘤周边血管内皮和窦内皮细胞表达VCAM－1阳性。肿瘤周边沉积大量呈阳性表达的FN与须大细胞紧密相联。结论：整合素α4β1及其配体VCAM－1和FN在肝肿瘤周边肥大细胞集要作用;整合素α4β1的表达水平与肿瘤周边MC数呈平行关系。     

饿蚂蝗化学成分研究

目的:研究饿蚂蝗Desmodium sambuens全草的化学成分。方法:采用硅胶、凝胶色谱法进行化学成分的分离纯化、通过理化性质和波谱分析进行结构鉴定。结果:从饿蚂蝗全草75%乙醇提取物中分离鉴定了13个化合物,结构分别为β-香树脂醇(1),白桦脂酸(2),胡萝卜苷(3),正三十烷酸(4),羽扇20(29)-烯-3-酮(5),二十四烷酸单甘油酯(6),豆甾-5-烯-3β-7α-二醇(7),脱镁叶绿素甲酯(8),邻羟基苯甲酸(9),β-谷甾醇(10),d-儿茶素(11),木犀草素(12),表没食子儿茶素(13)。结论:化合物1～13均为首次从饿蚂蝗植物中分离得到。     

The comparative study on ultrastructure and immunohistochemistry in AFP negative and positive hepatocellular carcinoma.

Summary: To comparatively investigate ultrastructural characteristics and expressions of AFP (alpha-fetoprotein) and Tn (Thomsen-Friedenreich-related antigen) protein in AFP negative (AFP-) and AFP positive (AFP+) primary hepatocellular carcinoma. Fourty-three cases of AFP- and AFP+ hepatocellular carcinoma (HCC) tissues and five cases of normal liver tissues were divided into three groups: control group (normal liver tissue, n=5); AFP+ HCC group (the serum AFP level was higher than 10 ng/ml, n = 22); AFP- HCC group (the serum AFP level was lower than 10 ng/ml, n=21). The ultrastructural morphology was studied by transmission electron microscopy, the expressions of AFP and Tn protein were detected by immunohistochemistry and cell image analysis. 1. The immunohistochemical study showed that (1) the expression intensity and positive rate of Tn protein in AFP- HCC group were markedly higher than that in AFP+ HCC group (P<0.01); (2) The expression intensity of AFP in AFP- HCC group was lower than that in AFP+ HCC group (P<0.01). 2. The transmission electron microscopy demonstrated that some AFP- HCC cells linked closely with each other, others dispersed loosely just as cultured cells, the remarkable morphologic features in AFP- HCC cells were simple organelles, but they were abundant in the free polyribosomes. In AFP+ HCC group, all the HCC cells linked closely together and were rich organelles in their cytoplasm, especially the rough endoplasmic reticula. In addition, mitochondria and Golgi complex were obviously observed. (1) The AFP and Tn protein had discrepancy distribution in AFP- and AFP+ HCC tissues, Tn protein may be one of the early diagnostic indicators in AFP- HCC; (2) The synthetic locations of the AFP and Tn protein were different in hepatocarcinoma cells by ultrastructural observation.     

Supravitale Morphologische Veränderungen der Skelettmuskulatur in frühen Postmortalen Zeitintervalen

Current Medical Science - Die morphologischen Veränderungen des mechanisch bzw. Elektrisch gereizten Skelettmuskels von 20 Leichen mit erhaltener supravitaler Reagibilitat in...     

P21 WAF1/Cip1 Gene Expression in Primary Human Hepatocellular Carcinoma and its Relationship with P53 Gene Mutation

Ｐ２１ＷＡＦ１／Ｃｉｐ１ｐｒｏｔｅｉｎ（ｐ２１）ｉｓｔｈｅｐｒｏｄｕｃｔｏｆｗａｆ１ｇｅｎｅ,ａｌｓｏｋｎｏｗｎａｓｃｉｐ１,ｓｄｉ１［１,２］．Ｐ２１ＷＡＦ１／Ｃｉｐ１ｉｓａｕｎｉｖｅｒｓａｌｉｎｈｉｂｉｔｏｒｏｆｃｙｃｌｉｎ－ｄｅｐｅｎｄｅｎｔｋ...     

The prevalence of stroke and related risk factors among residents aged ≥ 40 years in Chongqing,Southwest China

Journal of Public Health - China bears the largest global stroke burden, yet little is known about its rates in Chongqing, southwest China. We aimed to investigate the prevalence and related risk...     

二乙基亚硝胺诱发大鼠肝癌变过程中三种基因的原位表达

为了了解癌基因在实验性肝癌形成中的作用，我们应用原位杂交技术对大鼠肝组织在二乙基亚硝胺（ＤＥＮＡ）诱癌过程中Ｎ－ｒａｓ，ｃ－ｍｙｃ，Ｈ－ｒａｓ的表达进行了研究。结果表明，诱癌早期增生的肝细胞及变异肝细胞灶中即显示Ｎ－ｒａｓ和ｃ－ｍｙｃ的过度表达，并随增生肝细胞结节的形成和演进，二者过表达细胞增多且相伴而存。诱癌中期出现Ｈ－ｒａｓ的过表达。结果提示，Ｎ－ｒａｓ，ｃ－ｍｙｃ的异常表达是大鼠肝癌变中较早出现的分子改变，可能与肝癌的启动有关，也是肝癌形态发生的分子基础之一，二者具有协同作用。而Ｈ－ｒａｓ的异常表达则可能对癌前病变的发展具促进作用。同时也表明，肝细胞的恶性转化需要多个癌基因的协同作用。     

大鼠腹腔肥大细胞肿瘤坏死因子合成与释放的研究

应用免疫细胞化学技术及酶联免疫吸附试验（ELISA）对大鼠腹腔肥大细胞合成和释放肿瘤坏死因子（TNF）的状况进行了观测。结果:新鲜分离纯化的大鼠腹腔肥大细胞胞浆呈TNF蛋白阳性。单纯肥大细胞培养早期（2～4 h）,上清液中未能检测到TNF,随着培养时间延长,上清液中TNF含量增多;盐酸吗啡与肥大细胞共同培养2h时,上清液中出现可测TNF。四甲基偶氮唑盐（MTT）比色分析试验表明,肥大细胞培养上清液具体有TNF的抗肿瘤活性中。提示:大鼠腹腔肥大细胞能合成和释放TNF,盐酸吗啡能促进肥大细胞释放TNF。     

Studie über eine alte Leiche von der Zeit der Streitenden Reiche aus dem Grab Nr. 1 am Guo-Jia-Gang in Stadt Jingmen der Provinz Hubei

An ancient femal corpse excavated from the robbed tomb No. 1 at Guo-Jia-Gang in Jingmen city of Hubei Province was studied. It was confirmed that the corpse was buried more than 2 300 years ago, namely at the middle stage of the Warring States period and is the earliest one of the ancient coffin corpses unearthed in China sofar. It is of the same type as the ancient corpses of Weatern Han Dynasty excavated from tomb No. 1 at Ma-Wang-Dui in Changsha City and from the tomb No. 168 at Phoenix Hill in Jinagling City respectively. The collagenous fibers of connective tissue from all parts in the corpse were well preserved, showing fine ultrastructure with clear periodical cross banding, and a lot of bacterial spores were found between them. In addition, a large number of eggs ofclonorchis sinensis andtrichuris trichiura were found in the content of the bowel. The level and the cause of good preservation were discussed and it was believed that the good preservation may resulte from the combined effect of the internal and external environmental factors around the corpse.     

人肝癌组织nm23的表达及其与转移的关系

目的：研究ｎｍ２３基因在肝癌转移，发生，发展中的意义，方法：应用免疫组人技术（ＳＰ法）检测ｎｍ２３在２４例人肝癌组织的表达，结果ｎｍ２３在肝癌组织中较高表达，在癌旁组织中也有表达，在已发生临床转移的肝癌组织中ｎｍ２３的表达水平明显低于尚未发生转移，结论：ｎｍ２３在抑制肝癌转移中有一定的作用，但在肝癌转移过程显然尚有其他调节因素的存在。