组织特异性胞嘧啶脱氨酶和5-氟胞嘧啶热化疗对裸鼠结肠癌肝脏转移的影响

Objective To investigate the effects of tissue specific cytosine deaminase/5-fluorocytosine (CD/5-FC) thermotherapy on hepatic metastasis of colonic carcinoma in nude mice. Methods Forty-five nude mice were randomly divided into control group, 5-FC group and 5-FC thermotherapy group according to the random number table (15 mice in each group). Mice models of hepatic metastasis of colonic carcinoma were established by portal vein injection of LoVo/CEACD cells. The hepatic metastasis rate and number of metastatic nodules of the 3 groups were compared by ehi-square test and one-way ANOVA. The pathological changes in tumor tissues and apoptotic index of tumor cells were observed. The expression of the CD gene in tumor tissues was detected by fluorescent quantitative RT-PCR and Western blot. Results The number of metastatic nodules and liver metas-tasis rate were 4.6±1.3 and 100.0% in control group, 2.2±1.0 and 60.0% in 5-FC group, 0.5±0.8 and 13.3% in 5-FC thermotherapy group, with statistical difference among the 3 groups (F=25.898, χ2=5.208, 19.548, 5.168, P<0.05). The mean apoptotic indexes of tumor cells of the 3 groups were 4.6%, 9.9% and 17.4%, respectively. Vacuolar degeneration, cell necrosis, cytolysis and apoptotic bodies were mostly observed in the 5-FC thermotherapy group. The expression of CD gene in tumor tissue was detected in all the groups. Conclusion Tissue specific CD/5-FC thermotherapy has inhibitory effects on the hepatic metastasis of LoVo cells transfected with CD gene.     

直肠癌全直肠系膜切除术后直肠阴道瘘原因与防治

本文通过分析近5年来我院18例女性直肠癌全直肠系膜切除术后直肠阴道痿的临床资料。发现直肠阴道痿多发生于中低位直肠癌行全直肠系膜切除保肛手术的患者。痿的发生与肿瘤的位置、肿瘤的分期、肿瘤距肛门口的距离．以及手术技巧、手术难度、引流方法有关。而与是否进行预防性造口，以及是否采用腹腔镜手术无关。预防上强调应重视术中直肠前壁的锐性分离、结肠直肠吻合以及术后盆腔的负压引流。治疗上应首先进行保守治疗，即肠内营养，阴道冲洗。早期配合肠外营养、及全身或局部抗炎治疗，大部分直肠阴道痿通过保守治疗可以治愈。保守治疗无效时考虑结肠或回肠末端造口。若长期不愈应考虑吻合口肿瘤复发的可能。     

人核受体hLRH-1不同变异体在多种肿瘤组织和细胞系中的表达

目的:初步分析人核受体hLRH-1不同变异体在多种肿瘤组织和细胞系中的表达。方法:常规RT-PCR法分析hLRH-1v1和hLRH-1在肝癌等多种肿瘤组织和HepG2等肿瘤细胞系中的表达情况，实时定量PCR法分析hLRH-1v1，hLRH-1和hOct4在HepG2，SMMC-7721和BEL-7402三株肝癌细胞系的表达水平。结果:hLRH-1v1的表达见于所有检测的12种类型肿瘤和8种恶性肿瘤细胞系，hLRH-1的表达则仅在12种类型肿瘤中的6种肿瘤组织中可检测到，但8种恶性肿瘤细胞系均为hLRH-1阳性表达；在HepG2，SMMC-7721和BEL-7402三株肝癌细胞系中hLRH-1v1和hOct4的表达呈正相关。结论:hLRH-1v1在肿瘤中广泛表达，可能在维持肿瘤干细胞的自我更新中发挥重要作用。     

转自杀基因CD的LoVo细胞死亡机制的研究

目的　研究转染逆转录病毒载体 (G1CEACDNa)的肠癌细胞LoVo株的死亡机制。方法　脂质体法转染G1CEACDNa至LoVo细胞。TRIzol试剂提取总RNA ,行RT -PCR间接验证CD基因的表达情况。用含 1mmol·L-15 -FC的 164 0培养基培养细胞 ,MTT法测细胞生长曲线和旁观者效应 ;透射电镜观察细胞的超微结构 ;流式细胞仪annexinⅤ和PI双重染色行凋亡相关检查。结果　RT -PCR产物电泳显示在 1 5kb处可见相应的显影条带。MTT比色实验示 1mmol·L-15 -FC作用下CD+ LoVo细胞 48h开始生长抑制 ,72h、96h、12 0h抑制率分别约为 3 0 %、5 0 %、80 %。电镜下可见凋亡的早、中、晚期细胞及凋亡小体 ,同时可见部分细胞呈坏死改变。流式细胞仪显示 48h开始有少量细胞出现凋亡 ,72h、96h细胞群凋亡和坏死的比例分别为 2 3 8%、3 5 1%和 2 0 4%、2 6 0 %。结论　转染G1CEACDNa的LoVo细胞给予 5 -FC治疗 ,其细胞死亡机制为坏死和凋亡并存 ,凋亡相关过程的诱导可能是该治疗过程中旁观者效应的重要机制。     

乳腺癌治疗方法的变迁

乳腺癌是女性最常见的恶性肿瘤,全球每年约有近50万人死于该病。19世纪人们认为乳腺癌是一种局部性疾病,区域淋巴结是癌细胞通过的机械屏障,遵循时间与解剖学的规律,按照＂淋巴-血液＂的传播途径进行,即乳腺癌先经局部病变后发     

粘贴式负压冲吸器在合并切口裂开的高流量肠外瘘治疗中的应用

肠外瘘是胃肠道术后的严重并发症，高流量肠外瘘…合并切口裂开时如何有效地控制和收集流出的肠液，使切口和周围皮肤不受污染和刺激，这是临床上处理肠外瘘的难题。对于此类患者，普遍采用的措施是开放式滴水双套管负压吸。然而，此方法存在较多问题，结果不尽人意。作者自2002年4月至2006年8月对44例合并伤口裂开的高流量肠外瘘患者采用粘贴式负压冲吸器治疗，与1996年2月至2002年3月治疗的38例同类患者在相比。在治愈率、伤口换药次数、敷料消耗量、伤口愈合时间、[第一段]     

组织特异性胞嘧啶脱氨酶和5-氟胞嘧啶热化疗对裸鼠结肠癌肝脏转移的影响

Objective To investigate the effects of tissue specific cytosine deaminase/5-fluorocytosine (CD/5-FC) thermotherapy on hepatic metastasis of colonic carcinoma in nude mice. Methods Forty-five nude mice were randomly divided into control group, 5-FC group and 5-FC thermotherapy group according to the random number table (15 mice in each group). Mice models of hepatic metastasis of colonic carcinoma were established by portal vein injection of LoVo/CEACD cells. The hepatic metastasis rate and number of metastatic nodules of the 3 groups were compared by ehi-square test and one-way ANOVA. The pathological changes in tumor tissues and apoptotic index of tumor cells were observed. The expression of the CD gene in tumor tissues was detected by fluorescent quantitative RT-PCR and Western blot. Results The number of metastatic nodules and liver metas-tasis rate were 4.6±1.3 and 100.0% in control group, 2.2±1.0 and 60.0% in 5-FC group, 0.5±0.8 and 13.3% in 5-FC thermotherapy group, with statistical difference among the 3 groups (F=25.898, χ2=5.208, 19.548, 5.168, P<0.05). The mean apoptotic indexes of tumor cells of the 3 groups were 4.6%, 9.9% and 17.4%, respectively. Vacuolar degeneration, cell necrosis, cytolysis and apoptotic bodies were mostly observed in the 5-FC thermotherapy group. The expression of CD gene in tumor tissue was detected in all the groups. Conclusion Tissue specific CD/5-FC thermotherapy has inhibitory effects on the hepatic metastasis of LoVo cells transfected with CD gene.     

小肠血管瘤6例报告

本文对本院1981年～2001年收治的6例小肠血管瘤患者进行回顾性分析.现报告如下. 一、临床资料: 本组6例,男4例,女2例,年龄最小24岁,最大47岁.其中5例有慢性消化道出血,及反复黑便史,2例伴突发大出血,慢性出血5例均有贫血、乏力、头晕之症状,其中2例大出血急诊手术病人术前边抢救边行选择性肠系膜血管造影术,找到出血部位急诊手术,1例大出血因病情危急,未经检查,剖腹探查,术中找到病灶并切除,术后病理证实诊断.另4例慢性出血病人2例经锝99mTC体外标记红细胞后注入静脉内找到腹腔出血位置,另2例行血管造影确诊.     

黄芪注射液诱导人结肠癌Lovo细胞凋亡机制的研究

目的研究黄芪注射液诱导结肠癌Lovo细胞株的凋亡机制。方法以不同浓度的黄芪注射液作用于人结肠癌Lovo细胞株,用MTT法测黄芪注射液对Lovo细胞体外增殖的影响,透射电镜观察细胞超微结构;流式细胞仪AnnexinV和PI双重染色行凋亡相关检查。结果与对照组相比,实验组对Lovo细胞株增殖均有明显的抑制作用,且与浓度和作用时间呈正比;电镜下可见凋亡的早、中、晚期细胞及凋亡小体,同时可见部分细胞呈坏死改变。流式细胞仪显示:黄芪注射液作用Lovo细胞48 h后,0.05 g/mL、0.1 g/mL、0.15 g/mL、0.2g/mL各组的凋亡率分别为4.8%,18.6%,42.1%,64.8%。结论黄芪注射液能抑制Lovo细胞生长,诱导细胞凋亡。