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| 人核受体hLRH-1不同变异体在多种肿瘤组织和细胞系中的表达 | |
| 引用本文： | 邓小军,王水良,兰风华,柯龙凤,黎成金,曾志勇.人核受体hLRH-1不同变异体在多种肿瘤组织和细胞系中的表达[J].国际病理科学与临床杂志,2007,27(6):3-480. |
| 作者姓名： | 邓小军 王水良 兰风华 柯龙凤 黎成金 曾志勇 |
| 作者单位： | 1. 南京军区福州总医院全军检验医学研究所分子医学研究中心,福州,350025 2. 南京军区福州总医院普通外科,福州,350025 3. 南京军区福州总医院心胸外科,福州,350025 |
| 基金项目： | 福建省青年科技人才创新项目(2004J067)，福建省自然科学基金计划资助项目(C0710038)，福州总医院院立课题资助项目(200638)~~ |
| 摘 要： | 目的:初步分析人核受体hLRH-1不同变异体在多种肿瘤组织和细胞系中的表达。方法:常规RT-PCR法分析hLRH-1v1和hLRH-1在肝癌等多种肿瘤组织和HepG2等肿瘤细胞系中的表达情况，实时定量PCR法分析hLRH-1v1，hLRH-1和hOct4在HepG2，SMMC-7721和BEL-7402三株肝癌细胞系的表达水平。结果:hLRH-1v1的表达见于所有检测的12种类型肿瘤和8种恶性肿瘤细胞系，hLRH-1的表达则仅在12种类型肿瘤中的6种肿瘤组织中可检测到，但8种恶性肿瘤细胞系均为hLRH-1阳性表达；在HepG2，SMMC-7721和BEL-7402三株肝癌细胞系中hLRH-1v1和hOct4的表达呈正相关。结论:hLRH-1v1在肿瘤中广泛表达，可能在维持肿瘤干细胞的自我更新中发挥重要作用。 |
| 关 键 词： | 核受体hLRH-1 变异体 肿瘤 基因表达分析 |
| 文章编号： | 1673-2588(2007)06-0476-05 |
| 收稿时间： | 2007-7-4 |
| 修稿时间： | 2007年7月4日 |
Expressions of different variants of human nuclear receptorhLRH-1 in multiple cancer tissues and cell lines |
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| DENG Xiao-jun,WANG Shui-liang,LAN Feng-hua,KE Long-feng,LI Cheng-jin,ZENG Zhi-yong.Expressions of different variants of human nuclear receptorhLRH-1 in multiple cancer tissues and cell lines[J].Journal of International Pathology and Clinical Medicine,2007,27(6):3-480. | |
| Authors: | DENG Xiao-jun WANG Shui-liang LAN Feng-hua KE Long-feng LI Cheng-jin ZENG Zhi-yong |
| Affiliation: | 1.Center for Molecular Medicine, Institute of Laboratory Medicine; 2.Department of General Surgery;3.Department of Cardiothoracic Surgery, Fuzhou General Hospital, Fuzhou 350025, China |
| Abstract: | Objective To investigate the expression profiles of different isoforms of hLRH-1 in multiple cancer tissues and cell lines.Methods Regular RT-PCR was carried out to analyze the expressions of hLRH-1v1 and hLRH-1 in multiple cancer tissues including hepatocellular carcinoma,and cancer cell lines such as HepG2.The relative mRNA abundance of hLRH-1v1,hLRH-1 and hOct4 in HepG2,SMMC-7721 and BEL-7402 was also analyzed by real time quantitative PCR.Results The hLRH-1v1 was widely expressed in all detected 12 types of cancer tissues and 8 cancer cell lines,while hLRH-1 was expressed only in 6 out of 12 types of cancer tissues,although all 8 cancer cell lines were shown to have expression of hLRH-1.In HepG2,SMMC-7721 and BEL-7402,the expression level of hLRH-1v1 was correlated with that of hOct4.Conclusion hLRH-1v1 is generally expressed in tumors where it may play important role in self-renewal of cancer stem cell. |
| Keywords: | nuclear receptor hLRH-1 variant tumor analysis of gene expression |
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