Effects of haloperidol on communicative and aggressive behavior in male mice with different experiences of aggression.

Effects of two doses of haloperidol (0.1 and 0.4 mg/kg, 30 min and 24 h, IP) on communicative and aggressive behavior in C57BL/6J male mice have been studied. Some of the mice were without prior experience of aggression ("recruits"); the others had been victorious in 20 daily aggressive confrontations ("experienced winners"). Communicative behavior was estimated as the behavioral reaction to a standard tester (loser) in the partition test. Haloperidol in either dose significantly reduced communicative behavior in the "recruits." but not in the "experienced winners." Significantly fewer attacks, less total attacking time, and total time of aggressive behavior (aggressive grooming + attacks) were demonstrated by the "experiences winners," than by the "recruits," while the latency of the first attack, the number, the total and average duration of aggressive grooming events were significantly higher. In the "recruits," haloperidol dose dependently increased the latency and decreased the number of attacks, the total attacking time, and the total time of aggressive behavior 30 min and 24 h after injection. However, haloperidol did not affect the average or total time of aggressive grooming. Neither dose significantly affected any measure of aggressive behavior in the "experienced winners." It has been concluded that repeated aggression experience reduces the pharmacological sensitivity of the dopamine receptors.     

Post-Irradiation Recovery of B14-150 Fibrosarcoma Cells after Combined Irradiation with Low and High Linear Energy Transfer

Bulletin of Experimental Biology and Medicine - The use of radiation with low and high linear energy transfer (LET) in the same treatment regimen is promising in terms of increasing the efficiency...     

Study of the toxic and hemolytic properties of sera in the massive transfusion syndrome

The toxic and hemolytic properties of the sera of animals receiving massive transfusions of homologous blood were studied in experiments on 20 dogs and 432 mice. The toxicity of the recipients' sera was estimated in vivo in mice whose reticuloendothelial system had been blocked by Trypan Blue by calculating the mortality in per cent, and in vitro by the blood culture method. Hemolytic activity was studied by Dacie's principle with the use of erythrocytes labeled with radioactive chromium. The lost blood (45–50 ml/kg) was replaced in a volume 1.5 times greater than the volume of blood removed. The experiments showed that acute blood loss followed by massive transfusion of homologous blood caused the formation of toxic products in the recipient's serum. These toxic properties were found by two biological tests. The maximal toxigenic action was found 2 h and during the first day after blood replacement. On the 17th day no toxic effect of the sera could be found. During a parallel investigation of the hemolytic properties of the serum, a marked increase in its activity was discovered. The highest level of hemolytic activity was reached 24 h after blood replacement. After 3 days the hemolytic properties of the serum no longer differed from what they were initially.Presented by Academician of the Academy of Medical Sciences of the USSR N. A. Fedorov.Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 82, No. 7, pp. 792–794, July, 1976.     

Separation and detection of all phosphoinositide isomers by ESI-MS

Phosphoinositides (PIs) play fundamental roles as signalling molecules in numerous cellular processes. Direct analysis of PIs is typically accomplished by metabolic labelling with ³H-inositol or inorganic ³²P followed by deacylation, ion-exchange chromatography and flow scintillation detection. This analysis is laborious, time-consuming, and involves massive amounts of radioactivity. To overcome these limitations we established a robust, non-radioactive LC–ESI–MS assay for the separation and analysis of deacylated PIs that allows discrimination of all isomers without the need for radioactive labelling. We applied the method to various cell types to study the PI levels upon specific stimulation.     

Experimental investigation of the immunotherapy of burns

The effect of convalescent burn serum on the toxic properties, level of activity of proteolytic enzymes, and morphological changes after burns was studied in experiments on rats. After burns the serum and organ extracts were found to acquire toxic properties, proteolytic enzyme activity was increased, and marked morphological changes developed. Injection of convalescent burn serum promoted detoxication, reduced the proteolytic enzyme activity distinctly, and reduced the severity of the morphological changes. Serum of healthy animals gave a much smaller therapeutic effect.Pathophysiological Laboratory, Central Institute of Hematology and Blood Transfusion. Biochemical Laboratory, A. V. Vishnevskii Institute of Surgery. Laboratory of Embryonic Histogenesis, Research Institute of Human Morphology, Academy of Medical Sciences of the USSR, Moscow. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 84, No. 11, pp. 534-537, November, 1977.