江苏南部农村12883例宫颈癌筛查结果分析

目的了解江苏南部农村地区妇女宫颈癌前病变和宫颈癌的发病情况,探寻适合农村大规模宫颈癌筛查的方法。方法选取2011年江苏盛泽镇农村已婚妇女12883例,采用宫颈刮片脱落细胞学检查,对结果为不典型鳞状细胞（ASCUS）及以上者进行阴道镜及宫颈活检。结果12883例中,宫颈刮片细胞学ASCUS 531例（4.1％）,不能排除高级别鳞状上皮内病变不典型鳞状细胞（ASC‐H ）38例（0.3％）,低度鳞状上皮内病变（LSIL ）66例（0.5％）,高度鳞状上皮内病变（HSIL ）64例（0.5％）。接受阴道镜下宫颈活检432例,炎性反应237例,宫颈上皮内瘤变（CIN ）1级95例,CIN 2级42例,CIN 3级50例,浸润癌8例。结论宫颈刮片细胞学检查适合农村地区的宫颈癌筛查,结合阴道镜下宫颈活检可减少宫颈癌的漏诊率。     

一级护理的适用对象护理内容及护理质量评价方法

对一级护理的适用对象、护理内容、护理质量评价方法等进行了综述.     

一次性橡胶手套在手术室预防压伤的应用效果分析

目的在手术室中对患者进行压伤的预防采取一次性橡胶手套,对其应用效果展开分析。方法本研究纳入的对象为我院2015年9月至2017年9月的手术室患者60例,随机平分为两组,在手术室中进行常规护理为参照组;进行常规手术护理的同时采用一次性橡胶手套进行预防压伤为研究组,对两组患者出现压伤的情况展开比较。结果数据表示,研究组出现压伤的患者例数明显少于参照组,进行统计学分析存在明显差别P0.05。结论在对手术室患者进行常规手术护理时可以采用一次性橡胶手套来进行压伤的预防,具有明显的预防效果。     

弓形虫GRA1基因的体外扩增、克隆及鉴定

目的　体外扩增弓形虫RH株致密颗粒蛋白GRA1基因 ,并构建真核表达质粒。方法　从接种了弓形虫RH株的小鼠腹水中收集、纯化速殖子 ,提取基因组DNA ;据已知的GRA1基因列 ,设计合成一对引物 ,并引入EcoRⅠ和BamHⅠ酶切位点。应用PCR技术 ,从弓形虫RH株基因组DNA中扩增GRA1基因片段。扩增目的基因片段经纯化、双酶切后 ,插入真核表达质粒pEGFP -N3中 ,转化大肠杆菌DH5α感受态细胞 ,于卡那霉素阳性LB平板上筛选阳性克隆。重组子经EcoRⅠ和BamHⅠ双酶切、PCR鉴定。结果　从弓形虫RH株基因组DNA中扩增出 785bp的GRA1基因片段 ,构建重组质粒pEGFPN3-GRA1,酶切和PCR鉴定产物大小均与预期值相符。 结论　成功地从弓形虫RH株基因组DNA中获取了GRA1基因 ,并构建了pEGFPN3-GRA1重组质粒。为重组质粒的进一步表达和核酸疫苗的研究创造了条件     

Effect of thyroid hormone level on the expression of synaptotagmin Ⅰ in adult rat hippocampus

Objective To observe the effect of different thyroid hormone level on the expression of synaptotagmin Ⅰ(Syt Ⅰ) in adult rat hippocampus. Methods All 28 adult male SD rats were assigned randomly into hypothyroid, hyperthyroid and control group, hypothyroid group was established by daily intraperitoneal injections with propylthiou raci(PTU, 10.0 mg/kg body weight) for 6 weeks and hyperthyroid group with L-Thyroxine (L-T4, 0.5 mg/kg body weight) for 3 weeks. Radioimmunity method was used to assay the levels of serum T3 and T4, immunohistochemical S-P technology to assay the levels of Syt Ⅰ protein in hippoeampus CA1, CA3 and dentate gyrus (DG). The layers analyzed in the different subfields include the polymorphic cell layer(the stratum oriens, SO), pyramidal cell layer(PCL), stratum radiatum (SR), lacunosum-molecular layer (SLM) in CA1 and CA3, granular cell layer(GL) and molecular layer(ML) in DG. Results The levels of serum T3 and T4[(0.34±0.12), (41.03± 11.37)nmol/L]in the hypothyroid rats were significantly lower than those in the control group[(0.65±0.15), (55.20±10.68)nmol/L, P < 0.01 or < 0.05], and the positive granule of Syt Ⅰ was significantly lower in PCL and SR of CA1 and CA3, GL of DG. The average optical value responsible for Syt Ⅰ immunoreactivity was obviously reduced in SO(0.048±0.007), PCL(0.299±0.035), SR(0.042±0.007), SLM(0.038±0.006) of CA1, PCL(0.085± 0.019), SR(0.040±0.011), SLM (0.038±0.006) of CA3, GL (0.076±0.019) of DG than normal controls (0.068± 0.014, 0.376±0.053, 0.053±0.008,0.056±0.009,0.118±0.026,0.052±0.010,0.053±0.009,0.099±0.015; P< 0.01 or < 0.05). Serum T3 and T4 levels [(1.43±0.30), (157.18±19.95)nmol/L]of hyperthyroid rats were significantly higher than those of control group(P < 0.01). The value was reduced in PCL(0.322±0.050), SR(0.039±0.006), SLM (0.042±0.006) of CA1, PCL(0.098±0.034), SR(0.046±0.013), SLM(0.046±0.010) of CA3 and GL(0.085± 0.024), ML (0.042±0.009) of DG (P < 0.05 or < 0.01). Conclusion Adult-onset of hypothyroidism and hyperthyroidism can reversibly decrease the expression of Syt Ⅰ in CA1, CA3 and DG regions of hippocampus.     

唾液腺内神经肽及内分泌属性研究

自Cohen等1960年在小鼠下颌下腺中检测出神经生长因子(NGF)和表皮生长因子(EGF)而获诺贝尔医学奖以来,人们在下颌下腺相继发现了转化生长因子、胰岛素、内皮素、白细胞介素等30余种生物活性物质.1994年Mathison等提出颈交感干-颌下腺轴的新概念,国外学者对此十分关注,将其与下丘脑-脑垂体-内分泌腺轴相提并论.     

L-选择素配体合成酶在卵巢癌组织的表达

达水平随临床分期增加而增加.结论 N-乙酰氨基葡萄糖-6-磺基转移酶作为L-选择索配体合成酶在卵巢癌的发生、发展、转移侵袭过程中发挥作用.     

妊娠合并先天性心脏病161例临床分析

近年来先天性心脏病(先心病)呈增多趋势,由于心内、外科技术的发展,使得先心病患者能够健康的牛长至生育年龄[1].因此,埘于妊娠合并先心病的管理尤为重要.本文回顾性分析161例妊娠合并先心病患者的临床资料报道如下.