Reconstruction analysis of honeybee colony collapse disorder modeling

Optimization and Engineering - The honeybee has a significant impact on industry and nature. In recent years, a mysterious disease make honeybees die, often losses reach 80–100% of the...     

Synthesis and structure of large single crystalline silver hexagonal microplates suitable for micromachining

We report a simple one-step synthesis method of large single crystalline Ag (111) hexagonal microplates with sharp edges and a size of up to tens of microns. Single silver crystals were produced by reduction silver nitrate aqueous solution with 4-(methylamino)phenol sulfate. Scanning and transmission electron microscopy, energy-dispersive X-ray spectroscopy, selected area electron diffraction and optical microscopy techniques were combined to characterize the crystals. It is shown that the microplates can be easily dispersed and transferred as single objects onto different substrates and subsequently used as a high quality plasmonic starting material for micromachining of future nanocomponents, using modern top-down techniques like focused-ion beam milling and gas injection deposition.     

Engraftment of Prevascularized,Tissue Engineered Constructs in a Novel Rabbit Segmental Bone Defect Model

The gold standard treatment of large segmental bone defects is autologous bone transfer, which suffers from low availability and additional morbidity. Tissue engineered bone able to engraft orthotopically and a suitable animal model for pre-clinical testing are direly needed. This study aimed to evaluate engraftment of tissue-engineered bone with different prevascularization strategies in a novel segmental defect model in the rabbit humerus. Decellularized bone matrix (Tutobone) seeded with bone marrow mesenchymal stromal cells was used directly orthotopically or combined with a vessel and inserted immediately (1-step) or only after six weeks of subcutaneous “incubation” (2-step). After 12 weeks, histological and radiological assessment was performed. Variable callus formation was observed. No bone formation or remodeling of the graft through TRAP positive osteoclasts could be detected. Instead, a variable amount of necrotic tissue formed. Although necrotic area correlated significantly with amount of vessels and the 2-step strategy had significantly more vessels than the 1-step strategy, no significant reduction of necrotic area was found. In conclusion, the animal model developed here represents a highly challenging situation, for which a suitable engineered bone graft with better prevascularization, better resorbability and higher osteogenicity has yet to be developed.     

6‐Dihydroparadol,a Ginger Constituent,Enhances Cholesterol Efflux from THP‐1‐Derived Macrophages

1 Scope

Ginger is reported to be used for the prevention and treatment of cardiovascular diseases (CVD). Cholesterol efflux from macrophage foam cells is an important process in reverse cholesterol transport, whose increase may help to prevent or treat CVD. In this study, we investigated the effects of 6‐dihydroparadol from ginger on macrophage cholesterol efflux.

2 Methods and results

We show that 6‐dihydroparadol concentration‐dependently enhances both apolipoprotein A1‐ and human plasma–mediated cholesterol efflux from cholesterol‐loaded THP‐1‐derived macrophages using macrophage cholesterol efflux assay. 6‐Dihydroparadol increases protein levels of both ATP‐binding cassette transporters A1 and G1 (ATP‐binding cassette transporter A1 [ABCA1] and ATP‐binding cassette transporter G1 [ABCG1]) according to Western blot analysis. The ABCA1 inhibitor probucol completely abolishes 6‐dihydroparadol‐enhanced cholesterol efflux. Furthermore, increased ABCA1 protein levels in the presence of 6‐dihydroparadol were associated with both increased ABCA1 mRNA levels and increased ABCA1 protein stability. Enhanced ABCG1 protein levels were only associated with increased protein stability. Increased ABCA1 protein stability appeared to be the result of a reduced proteasomal degradation of the transporter in the presence of 6‐dihydroparadol.

3 Conclusion

We identified 6‐dihydroparadol from ginger as a novel promoter of cholesterol efflux from macrophages that increases both ABCA1 and ABCG1 protein abundance. This newly identified bioactivity might contribute to the antiatherogenic effects of ginger.     

Static Analysis of Object References in RMI-Based Java Software

Distributed applications provide numerous advantages related to software performance, reliability, interoperability, and extensibility. This paper focuses on distributed Java programs built with the help of the remote method invocation (RMI) mechanism. We consider points-to analysis for such applications. Points-to analysis determines the objects pointed to by a reference variable or a reference object field. Such information plays a fundamental role as a prerequisite for many other static analyses. We present the first theoretical definition of points-to analysis for RMI-based Java applications, and we present an algorithm for implementing a flow- and context-insensitive points-to analysis for such applications. We also discuss the use of points-to information for corrupting call graph information, for understanding data dependencies due to remote memory locations, and for identifying opportunities for improving the performance of object serialization at remote calls. The work described in this paper solves one key problem for static analysis of RMI programs and provides a starting point for future work on improving the understanding, testing, verification, and performance of RMI-based software     

Precise Call Graphs for C Programs with Function Pointers

The use of pointers presents serious problems for software productivity tools for software understanding, restructuring, and testing. Pointers enable indirect memory accesses through pointer dereferences, as well as indirect procedure calls (e.g., through function pointers in C). Such indirect accesses and calls can be disambiguated with pointer analysis. In this paper we evaluate the precision of one specific pointer analysis (the FA pointer analysis by Zhang et al.) for the purposes of call graph construction for C programs with function pointers. The analysis is incorporated in a production-strength code-browsing tool from Siemens Corporate Research in which the program call graph is used as a primary tool for code understanding.The FA pointer analysis uses an inexpensive, almost-linear, flow- and context-insensitive algorithm. To measure analysis precision, we compare the call graph constructed by this analysis with the most precise call graph obtainable by a large category of existing pointer analyses. Surprisingly, for all our data programs the FA analysis achieves the best possible precision. This result indicates that for the purposes of call graph construction, inexpensive pointer analyses may provide precision comparable to the precision of expensive pointer analyses.     

Module embedding e]radenski@ga.unc.edu

This paper proposes a code reuse mechanism called module embedding that enables the building of new modules from existing ones through inheritance, overriding of procedures, and overriding of types; the paper also describes an implementation scheme for this mechanism. Module embedding is beneficial when modules and classes are used in combination and need to be extended together, or when modules are a more appropriate medium than classes.     

Sentinel: generating GUI tests for sensor leaks in Android and Android wear apps

Software Quality Journal - Due to the widespread use of Android devices and apps, it is important to develop tools and techniques to improve app quality and performance. Our work focuses on a...