Engraftment of Prevascularized,Tissue Engineered Constructs in a Novel Rabbit Segmental Bone Defect Model |
| |
Authors: | Alexandre Kaempfen Atanas Todorov Sinan Güven René D Largo Claude Jaquiéry Arnaud Scherberich Ivan Martin Dirk J Schaefer |
| |
Affiliation: | 1.Clinic for Plastic, Reconstructive, Aesthetic and Hand Surgery, University Hospital Basel, 4031 Basel, Switzerland; E-Mails: (R.D.L.); (D.J.S.);2.Institute for Surgical Research and Hospital Management, University Hospital Basel, 4031 Basel, Switzerland; E-Mails: (A.T.); (S.G.); (A.S.); (I.M.);3.Clinic for Oral and Cranio-Maxillofacial Surgery, University Hospital Basel, 4031 Basel, Switzerland; E-Mail: |
| |
Abstract: | The gold standard treatment of large segmental bone defects is autologous bone transfer, which suffers from low availability and additional morbidity. Tissue engineered bone able to engraft orthotopically and a suitable animal model for pre-clinical testing are direly needed. This study aimed to evaluate engraftment of tissue-engineered bone with different prevascularization strategies in a novel segmental defect model in the rabbit humerus. Decellularized bone matrix (Tutobone) seeded with bone marrow mesenchymal stromal cells was used directly orthotopically or combined with a vessel and inserted immediately (1-step) or only after six weeks of subcutaneous “incubation” (2-step). After 12 weeks, histological and radiological assessment was performed. Variable callus formation was observed. No bone formation or remodeling of the graft through TRAP positive osteoclasts could be detected. Instead, a variable amount of necrotic tissue formed. Although necrotic area correlated significantly with amount of vessels and the 2-step strategy had significantly more vessels than the 1-step strategy, no significant reduction of necrotic area was found. In conclusion, the animal model developed here represents a highly challenging situation, for which a suitable engineered bone graft with better prevascularization, better resorbability and higher osteogenicity has yet to be developed. |
| |
Keywords: | animal model decellularized bone tissue engineering osteosynthesis vascularization bone resorption |
|
|