基于蓝光光盘技术的黄曲霉毒素B1快速定量检测

提出了基于蓝光光盘（Blu-ray disc, BD）技术和间接竞争免疫反应原理的黄曲霉毒素B1（Aflatoxin B1,AFB1）的定量检测方法。文中采用NaOH溶液活化水解BD表面使生物分子固定在其表面,并结合二甲基硅氧烷（PDMS）微流控通道制备间接竞争免疫检测结构,通过纳米金标记/银染技术增强信号,利用标准光驱结合光盘数据质量诊断软件（PlexUtilities）分析光盘上反应条带的错误数与AFB1浓度之间的关系,实现了对AFB1的定量检测。同ELISA的检测结果进行对比,二者结果基本一致。该方法检测限达到0.5 ng/mL,检测范围为0.5~200 ng/mL,满足我国国标对食品中AFB1的限定标准。     

智能手机光谱仪的开发与水中六价铬的定量检测

光谱仪是进行生化检测与光谱分析时所需的基本仪器,近些年来,研究开发小型、便携式的光谱仪已经成为重要的研究方向。设计了基于智能手机的光谱检测方法及装置,该装置以手机内置LED灯为宽谱光源,以DVD光盘聚碳酸酯层的周期性凹槽结构作为衍射光栅,利用智能手机进行光谱分析。以水中Cr~(6+)为例,利用显色反应生成紫红色络合物,采用所开发装置结合分光光度法实现了水中Cr~(6+)的快速定量检测。检测结果与常用的分光光度计结果一致,检测限为0.02 mg/L,达到了国标对饮用水中Cr~(6+)含量的检测要求。     

基于智能手机数字图片比色法的生化检测技术研究进展

传统的生物分子检测方法通常都依赖于精密仪器以及专业的操作,在现场以及缺乏专业设备的地区很难开展。而即时检测(point-of-care, POC)旨在提供快速实时诊断,该技术在医疗诊断、食品安全检测、环境监测等领域发挥着越来越重要的作用。数字图片比色法作为一种新型的比色分析方法近年来被广泛地研究和应用,利用这种比色法进行比色分析时通过分析数字图片的相关信息就可以进行定量检测。随着智能手机的快速发展,大规模生产和普及,它们为数字图片比色法即时检测的应用提供了便携、经济、易操作的平台。基于此,本文将介绍课题组在智能手机生化检测技术的开发与应用方面所取得的进展。     

基于蓝光光盘扫描成像技术的高通量生物 分子点阵定量检测仪

蓝光光盘不仅可以作为超清电影的存储媒介,而且由于其优良的光学特性和纳米级别的表面平整度,可以替代传统硅片作为生化反应的基底材料,并且结合蓝光光驱的高精度光学读取和伺服系统,可以用于研发新型的高通量分析传感装置,光盘表面制备的生物反应点阵会对光驱激光束的正常反射形成干扰,从而使得光驱光电探测器接收到的激光强度相应降低,通过采集光盘扫描过程中光电探测器的光强值信号,经蓝光光盘扫描成像分析,实现了对生物分子的定量检测。文中在光盘表面打印不同大小和灰度的喷墨点阵,结果表明该成像技术的分辨率可达100μｍ,验证了系统高通量检测的特点和定量检测的可行性,在蓝光光盘表面制备了生物反应点阵,经成像分析实现了对链霉亲和素浓度的定量检测,检测限达到0.01mol/Ｌ。     

基于智能手机的超灵敏高精度pH检测

研究开发了一种新型的基于智能手机的pH检测方法及装置,可以在多种环境条件下对pH值进行高精度测量,具有快速、灵敏、便携的特点。为了避免光照环境对测量结果的影响,采用3D打印技术制备了外置的手机光学附件装置。该pH传感检测装置可以与市售的pH试纸条一起配套使用,测量精度达到了0.05。将实际样品pH值的测量与pH计检测结果进行了对比,结果表明基于智能手机的超灵敏高精度pH检测方法是可行的,不需要专门的培训或复杂的仪器,具有广泛的市场应用前景。     

Fabrication of ordered micro- and nano-scale patterns based on optical discs and nanoimprint

A simple method to fabricate one-dimensional(1-D) and two-dimensional(2-D) ordered micro- and nano-scale patterns is developed based on the original masters from optical discs, using nanoimprint technology and soft stamps. Polydimethylsiloxane(PDMS) was used to replicate the negative image of the 1-D grating pattern on the masters of CD-R, DVD-R and BD-R optical discs, respectively, and then the 1-D pattern on one of the PDMS stamps was transferred to a blank polycarbonate(PC) substrate by nanoimprint. The 2-D ordered patterns were fabricated by the second imprinting using another PDMS stamp. Different 2-D periodic patterns were obtained depending on the PDMS stamps and the angle between the two times of imprints. This method may provide a way for the fabrication of complex 2-D patterns using simple 1-D masters.     

Protonation effect on the nonlinear absorption, nonlinear refraction and optical limiting properties of tetraphenylporphyrin

Nonlinear optical properties of tetraphenylporphyrin (H2TPP) and protonated tetraphenylporphyrin (H4TPP2+) in toluene were investigated by Z-scan technique using a nanosecond laser with 5 ns pulse at 532 nm. Results show that H4TPP2+ exhibits weaker nonlinear refraction but enhanced reverse saturable absorption (RSA) and optical limiting performance in comparison with pristine H2TPP. Since no nonlinear scattering is observed in H4TPP2+ under low input fluence, and H4TPP2+ exhibits weaker nonlinear scattering signals than H2TPP under high input fluence, the enhancement of RSA and optical limiting performance can be attributed to the larger ratio of excited state absorption cross-section to that of the ground state of H4TPP2+. H4TPP2+ also exhibits superior optical limiting performance, even better than the benchmark RSA material C60.     

基于光雕技术的还原氧化石墨烯压力传感器制备

石墨烯目前已被广泛用于压力传感器的制备,但由于传统石墨烯制备方法的制约,大部分已报道的压力传感器都只在较低范围内具有高灵敏度,无法用于宽范围的压力检测。文中将氧化石墨烯涂覆在贴有柔性薄膜的光雕光盘表面,通过光雕技术将氧化石墨烯进行了图案化还原,并将其用于制备测量宽压力范围的压力传感器。结果表明,此压力传感器对0~500 k Pa的压力范围有很好的线性响应,并且具有良好的稳定性。     

Study on optical nonlinearity and optical limiting property of porphyrin-oxygenated carbon nanomaterial blends

The goal of Optoelectronics Letters is to rapidly report original,new and important results in the fields of photonics and optoelec-tronics in English,to advance the international academic exchanges.Optoelectronics Letters pays a particularly attention to the cross topics between photonics and electronics.The scope of Optoelectronics Letters is as follows:Novel OEdevices and applications;New materials and functional components;Nano-structures and OE integration;Nano-quantum optoelectronics;Optoelectronic information technology;Sensing,measurement and inspection;Storage and display;Image and information processing;Biomedical photonics,etc.