北五味子的超临界萃取及产物的HPLC-APCI-MS/MS分析

优化超临界流体萃取北五味子中木脂素类成分的提取工艺,对萃取物中所含有效成分的归属进行分析鉴定.以五味子总木脂素为评价指标,采用正交实验法和不同影响因素系统考察法对CO2 -SFE萃取北五味子中木脂素类成分提取工艺进行优化,利用液相色谱-大气压化学电离质谱联用法(HPLC-APCI-MS/MS)分析鉴定产物结构.确定了最...     

微透析-液相色谱-质谱联用技术在中药研究中的应用

微透析-液相色谱-质谱联用技术不仅可以实现活体、实时、连续地微创取样,还具有高灵敏度、分析速度快、检测范围宽等特点,是中药研究的利器,已广泛应用于中药体内过程及作用机制研究等方面。本文对微透析技术进行了简要介绍,并结合相关研究实例,着重介绍了微透析-液相色谱-质谱联用技术在中药活性成分筛选、体内外代谢、药代动力学、代谢组学等研究中的策略及应用。     

液质联用技术分析精氨酸双糖苷对麦芽糖酶的抑制活性

Maltulosyl arginine is the initial maillard reaction product of maltose and arginine, which may have anti-diabetic activity. In this study, LC/MS was combined with ultrafiltration as a new method to investigate its inhibitory activity on maltase. The quantitative data was got from a triple quadrupole instrument in multiple reaction monitoring (MRM) mode. The experimental results proved that maltulosyl arginine is an effective maltase inhibitor and can be used for the treatment of diabetes and obesity.     

Semi-LC/MS技术快速定量分析黄芪样品中黄芪甲苷含量

为了测定黄芪中的黄芪甲苷含量,应用半高效液相色谱-质谱串联技术（Semi-LC/MS）,以薯蓣皂苷为内标化合物,仅用一段色谱保护柱分离掉大部分干扰性成分,同时使目标化合物富集,采用Waters公司的多反应监测（MRM）扫描模式检测。结果表明：黄芪甲苷在2.85~57.0 mg/L的范围内线性关系良好,稳定性和重复性实验RSD<3%,样品回收率达98.9%。Semi-LC/MS方法可以对黄芪样品中黄芪甲苷进行快速定量分析,是一种黄芪甲苷的简单、高效、灵敏的定量检测方法。     

三聚氰胺的基质辅助激光解吸/电离飞行时间质谱分析

Oxidized carbon nanotubes are tested as the matrix for analysis of the melamine by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). Traditional MALDI matrix are not suit for analysis of the low molecular compounds due to the interference associated to the matrix clusters. Oxidized carbon nanotubes can transfer energy to the analyte under the laser irradiation, which makes analyte well ionized or desorbed. Moreover, the interference of the intrinsic matrix ions can be eliminated. Melamine as the a toxic additive which had been added in the milk powder, then it is necessary to establish a new method for detection of the melamine rapid and sensitive.     

酒黄连的电喷雾质谱研究

Using ESI-MS, adding of different weight yellow wine and different roasting temperature of prepared Coptidis Rhizoma were investigated. The results were that the influence adding of different weight yellow wine on the content of alkaloids was little, but the roasting temperature was reverse. This study offers a perspective of the technique of prepared Coptidis Rhizoma .     

利用电喷雾质谱技术研究复方中药的配伍原则

以天然产物为基础的传统中药是中华文化的重要组成部分.由于复方中药组成的复杂性,给有效成分和疗效的研究带来很大的困难.本研究应用电喷雾电离(ESI),串联质谱(MSn)及高效液相色谱(HPLC)等技术,通过气相离子反应研究复方中药煎煮过程中发生的化学反应,进而揭示复方中药的配伍原则及解毒机理.     

白山草乌中二萜生物碱的电喷雾串联质谱分析

利用电喷雾串联质谱 ( ESI-MS2 )对吉林省白山市地产草乌的乙醇提取液进行了直接分析 ,方法简便 ,直观 ,用样量少。ESI-MS可以给出分子量信息 ,MS2 方法则可以从复杂体系中获得结构信息。在该植物中发现中乌头碱、次乌头碱乌头碱及中乌头碱的水解产物和脂类生物碱等九种二萜生物碱     

马钱苷的电喷雾串联质谱及傅立叶变换离子回旋共振质谱研究

利用电喷雾串联质谱（ESI-MSⁿ）技术研究马钱苷的质谱碎裂机理, 探讨其碎裂方式,并利用傅立叶变换离子回旋共振质谱（FTICR-MS）及SORI-CID高分辨率、高质量准确度对其碎裂机理进行验证研究。采用 H/D 交换和串联质谱的方法,发现马钱苷羟基上的活泼氢在质谱中性碎片丢失过程中较易被转移,表现出较大的化学活性。通过加入特定的Na⁺ 和 Li⁺ 等碱金属离子,极大地提高了马钱苷化合物质谱分析的灵敏度,获得了更多的结构信息,建立了马钱苷化合物质谱鉴定和分析的新方法。     

金属离子与钙调蛋白相互作用的基质辅助激光解吸电离飞行时间质谱研究

Calmodulin is a ubiquitous calcium-binding protein in eukaryote cells and engages in various important biological pathways. In the present study, binding interactions between several metal ions and calmodulin were investigated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The results revealed that the specific binding of metal ions with the protein could be detected using MALDI-TOF MS.