| Semi-LC/MS技术快速定量分析黄芪样品中黄芪甲苷含量 |
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| 引用本文： | 郑重,宋凤瑞,刘舒,赵先恩,刘志强,刘淑莹.Semi-LC/MS技术快速定量分析黄芪样品中黄芪甲苷含量[J].质谱学报,2012,33(4):208-211. |
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| 作者姓名： | 郑重 宋凤瑞 刘舒 赵先恩 刘志强 刘淑莹 |
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| 作者单位： | 1. 中国科学院长春应用化学研究所，长春质谱中心，吉林 长春130022；2. 中国科学院研究生院，北京100049 |
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| 基金项目： | 国家自然科学基金(20953001);吉林省与中国科学院科技合作资金(2010SYHZ0052,2011CJT0015)项目资助 |
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| 摘 要： | 为了测定黄芪中的黄芪甲苷含量，应用半高效液相色谱-质谱串联技术（Semi-LC/MS），以薯蓣皂苷为内标化合物，仅用一段色谱保护柱分离掉大部分干扰性成分，同时使目标化合物富集，采用Waters公司的多反应监测（MRM）扫描模式检测。结果表明：黄芪甲苷在2.85~57.0 mg/L的范围内线性关系良好，稳定性和重复性实验RSD<3%，样品回收率达98.9%。Semi-LC/MS方法可以对黄芪样品中黄芪甲苷进行快速定量分析，是一种黄芪甲苷的简单、高效、灵敏的定量检测方法。
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| 关 键 词： | 黄芪甲苷 半高效液相色谱-质谱串联技术（Semi-LC/MS） 多反应监测（MRM） 定量分析 |
Rapid Quantitative Analysis of Astragaloside in the Astragalus Samples by Semi-LC/MS |
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| ZHENG Zhong
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SONG Feng-rui
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LIU Shu
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ZHAO Xian-en
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LIU Zhi-qiang
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LIU Shu-ying.Rapid Quantitative Analysis of Astragaloside in the Astragalus Samples by Semi-LC/MS[J].Journal of Chinese Mass Spectrometry Society,2012,33(4):208-211. |
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| Authors: | ZHENG Zhong SONG Feng-rui LIU Shu ZHAO Xian-en LIU Zhi-qiang LIU Shu-ying |
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| Affiliation: | 1. Changchun Institute of Applied Chemistry, Chinese Academy of Science，Changchun 130022, China；2. Graduate School of Chinese Academy of Science，Beijing 100049, China |
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| Abstract: | In order to determinate the astragaloside in the astragalus by Semi-LC/MS,diosgenin is used as an internal standard compound,a chromatography guard column was used to remove most interference components and enrich the target compound which was subsequently determined by MS under multiple reaction monitoring(MRM) scanning-mode.The calibration curve is linear over the range of 2.85—57.0 mg/L of astragaloside.The RSDs for the stability and reproducibility experiments are less than 3%,and sample recovery rate is 98.9%.Semi-LC/MS is one simple,efficient,sensitive method for rapid quantitative analysis of astragaloside in astragalus samples. |
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| Keywords: | Astragaloside semi-high performance liquid chromatography-mass spectrometry(Semi-LC/MS) multiple reaction monitoring(MRM) quantitative analysis |
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