Fabrication and Thermal Dissipation Properties of Carbon Nanofibers Derived from Electrospun Poly(Amic Acid) Carboxylate Salt Nanofibers

Polyimides (PIs) possess excellent mechanical properties, thermal stability, and chemical resistance and can be converted to carbon materials by thermal carbonization. The preparation of carbon nanomaterials by carbonizing PI‐based nanomaterials, however, has been less studied. In this work, the fabrication of PI nanofibers is investigated using electrospinning and their transformation to carbon nanofibers. Poly(amic acid) carboxylate salts (PAASs) solutions are first electrospun to form PAAS nanofibers. After the imidization and carbonization processes, PI and carbon nanofibers can then be obtained, respectively. The Raman spectra reveal that the carbon nanofibers are partially graphitized by the carbonization process. The diameters of the PI nanofibers are observed to be smaller than those of the PAAS nanofibers because of the formation of the more densely packed structures after the imidization processes; the diameters of the carbon nanofibers remain similar to those of the PI nanofibers after the carbonization process. The thermal dissipation behaviors of the PI and carbon nanofibers are also examined. The infrared images indicate that the transfer rates of thermal energy for the carbon nanofibers are higher than those for the PI nanofibers, due to the better thermal conductivity of carbon caused by the covalent sp² bonding between carbon atoms.     

Betel Nut Arecoline Induces Different Phases of Growth Arrest between Normal and Cancerous Prostate Cells through the Reactive Oxygen Species Pathway

Prostate cancer (PCa) is a reproductive system cancer in elderly men. We investigated the effects of betel nut arecoline on the growth of normal and cancerous prostate cells. Normal RWPE-1 prostate epithelial cells, androgen-independent PC-3 PCa cells, and androgen-dependent LNCaP PCa cells were used. Arecoline inhibited their growth in dose- and time-dependent manners. Arecoline caused RWPE-1 and PC-3 cell cycle arrest in the G2/M phase and LNCaP cell arrest in the G0/G1 phase. In RWPE-1 cells, arecoline increased the expression of cyclin-dependent kinase (CDK)-1, p21, and cyclins B1 and D3, decreased the expression of CDK2, and had no effects on CDK4 and cyclin D1 expression. In PC-3 cells, arecoline decreased CDK1, CDK2, CDK4, p21, p27, and cyclin D1 and D3 protein expression and increased cyclin B1 protein expression. In LNCaP cells, arecoline decreased CDK2, CDK4, and cyclin D1 expression; increased p21, p27, and cyclin D3 expression; had no effects on CDK1 and cyclin B1 expression. The antioxidant N-acetylcysteine blocked the arecoline-induced increase in reactive oxygen species production, decreased cell viability, altered the cell cycle, and changed the cell cycle regulatory protein levels. Thus, arecoline oxidant exerts differential effects on the cell cycle through modulations of regulatory proteins.     

Outer Membrane Vesicle Production by Helicobacter pylori Represents an Approach for the Delivery of Virulence Factors CagA,VacA and UreA into Human Gastric Adenocarcinoma (AGS) Cells

Helicobacter pylori infection is the etiology of several gastric-related diseases including gastric cancer. Cytotoxin associated gene A (CagA), vacuolating cytotoxin A (VacA) and α-subunit of urease (UreA) are three major virulence factors of H. pylori, and each of them has a distinct entry pathway and pathogenic mechanism during bacterial infection. H. pylori can shed outer membrane vesicles (OMVs). Therefore, it would be interesting to explore the production kinetics of H. pylori OMVs and its connection with the entry of key virulence factors into host cells. Here, we isolated OMVs from H. pylori 26,695 strain and characterized their properties and interaction kinetics with human gastric adenocarcinoma (AGS) cells. We found that the generation of OMVs and the presence of CagA, VacA and UreA in OMVs were a lasting event throughout different phases of bacterial growth. H. pylori OMVs entered AGS cells mainly through macropinocytosis/phagocytosis. Furthermore, CagA, VacA and UreA could enter AGS cells via OMVs and the treatment with H. pylori OMVs would cause cell death. Comparison of H. pylori 26,695 and clinical strains suggested that the production and characteristics of OMVs are not only limited to laboratory strains commonly in use, but a general phenomenon to most H. pylori strains.     

SOINN-Based Abnormal Trajectory Detection for Efficient Video Condensation

With the evolution of video surveillance systems, the requirement of video storage grows rapidly; in addition, safe guards and forensic officers spend a great deal of time observing surveillance videos to find abnormal events. As most of the scene in the surveillance video are redundant and contains no information needs attention, we propose a video condensation method to summarize the abnormal events in the video by rearranging the moving trajectory and sort them by the degree of anomaly. Our goal is to improve the condensation rate to reduce more storage size, and increase the accuracy in abnormal detection. As the trajectory feature is the key to both goals, in this paper, a new method for feature extraction of moving object trajectory is proposed, and we use the SOINN (Self-Organizing Incremental Neural Network) method to accomplish a high accuracy abnormal detection. In the results, our method is able to shirk the video size to 10% storage size of the original video, and achieves 95% accuracy of abnormal event detection, which shows our method is useful and applicable to the surveillance industry.     

External stimulus-responsive biomaterials designed for the culture and differentiation of ES,iPS, and adult stem cells

The physical and chemical characteristics of biomaterial surface and hydrogels can be altered by external stimuli, such as light irradiation, temperature changes, pH shifts, shear stress forces, electrical forces, and the addition of small chemical molecules. Such external stimulus-responsive biomaterials represent promising candidates that have been developed for the culture and differentiation of embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, and adult stem cells. Biomaterials that are designed to respond in a reversible manner to specific external signals can be formed on micropatterned or non-micropatterned surface, in hydrogels, or on microcarriers. Stem cells and the cells differentiated from them into specific tissue lineages can be cultured and/or differentiated on dishes with immobilized external stimulus-responsive polymers. Cells can be detached from these dishes without using an enzymatic digestion method or a mechanical method when the appropriate external stimulus is generated on the surface. This review discusses the polymers and polymeric designs employed to produce surface and hydrogels for stem cell culture, differentiation, and/or cell detachment using various external stimuli.     

分析位于沉积地层中建物产生过大沉降量之新观念

在软弱地基土层上设计建筑物时，除了考虑基础下土层的承载力必须达到安全要求外，地基土的长期沉降变形也同样重要。本文在追踪一工程实例中，发现依据传统土力学观念在深厚沉积土层中，标准贯入击数Ｎ值已达１５～２５之地层，设计４５ｍ长摩擦桩基础之谷仓结构物，在使用１９年后仍产生因差异沉降过量导致之严重结构破坏。传统土力学较偏于可靠的承载力，对于各土层的先期固结应力，仅能由单向度固结试验求得，但实际工作时，取得完全不扰动土样几乎不可能，因而亦很难取得正确的先期固结应力，便无法计算正确沉降量。故设计者常因本身主观的观念，造成研判的疏忽。本文提出“由Ｎ值判断各土层超固结比（ＯＣＲ）法及应力还原法”观念，由不同深度土层的标准贯入击数Ｎ值及地质历史，可协助设计者于设计阶段便能轻易而明确地判断不同深度土层的固结比（ＯＣＲ），精确计算各土层沉降量，以便能事先设计出防止类似本实例于１９年后才发现的疏忽。     

Numerical investigation of airflow pattern and particulate matter transport in naturally ventilated multi-room buildings

This study reports on a numerical investigation of transport behavior of indoor airflow and size-dependent particulate matter (PM) in multi-room buildings. An indoor size-dependent PM transport approach, combining the Eulerian large-eddy simulation of turbulent flow with the Lagrangian particle trajectory tracking, was developed to investigate indoor airflow pattern and PM1/PM2.5/PM10 removal efficiency in naturally ventilated multi-room buildings. A displacement ventilation with a measured indoor PM10 profile in Taipei Metropolis as the initial condition was carried out to characterize spatial and temporal variations of indoor PM1/PM2.5/PM10 removal behavior. The effects of indoor airflow pattern on particle transport mechanisms, e.g., deposition, suspension, migration and escape, were analyzed. Two comparison scenarios, which considered the effects of no indoor partition and different air change rate, respectively, were also conducted. In comparison with the effectiveness of PM1/PM2.5/PM10 removal, the simulated results showed that coarse particles were easier to be removed out of the building than fine particles. Natural ventilation was not an effective way to remove fine particles such as PM1 and PM2.5 in a multi-room building. Indoor partitions can impede 12% of the mean streamwise velocities and significantly increase 30-50% turbulence intensities. However, indoor partitions increased particle deposition and decreased particle escape. As a result of the two opposite particle removal mechanisms, i.e., deposition and escape, the impact of indoor partitions on PM1/PM2.5/PM10 removal behavior was not as significant as the results of airflow velocities. PRACTICAL IMPLICATIONS: This work developed a computational fluid dynamics technique to investigate indoor airflow patterns and PM1/PM2.5/PM10 removal ability in ventilated multi-room buildings. The results of this paper can help to identify adequate PM1/PM2.5/PM10 cleaning procedure and provide useful size-dependent PM control strategy in multi-room buildings.