核酸环介导等温扩增技术

介绍环介导等温扩增技术的基本原理、特点及应用前景。环介导等温扩增技术具有简单、快速、特异性强和扩增效率高等特点,目前可检测乙型肝炎病毒、流感病毒、疱疹病毒、水痘-带状疱疹病毒、腮腺炎病毒、麻疹病毒、腺病毒、SARS冠状病毒、呼吸道合胞病毒、西尼罗河病毒、结核分支杆菌、痢疾志贺菌、大肠杆菌、螺旋体、肺炎链球菌和耶氏菌等。     

Effect of insulin on the differential expression of VLDL receptor isoforms of SGC7901 cell and its biological implication

This study examined the effect of insulin on the expression of very low density lipoprotein receptor (VLDLR) subtypes of SGC7901 cells and discussed its biological implication.In vitro, moderately or poorly-differentiated human gastric adenocarcinoma cell line SGC7901 was incubated with insulin for different lengths of time, and then the expression of protein and RNA level in VLDLR subtypes were detected by Western blotting and real-time PCR, respectively.The results showed that, at certain time interval, insulin could down-regulate expression of type Ⅰ VLDLR and up-regulate the expression of type Ⅱ VLDLR in SGC7901 cells, at both protein and RNA level.We are led to conclude that insulin serves as a regulator in maintaining the balance between glucose and lipid metabolism in vivo, possibly through its effect on the differential expression of VLDLR subtypes.     

PBL联合CBL教学法在住院医师规范化培训心电图临床教学中的应用研究

探索PBL联合CBL教学法在心内科住院医师规范化培训学员心电图临床教学的教学效果.与传统LBL教学相比,PBL联合CBL教学显著提高学员心电图判读成绩,并显著提高学员的学习兴趣、理论掌握、应用能力及主动学习能力.     

环介导等温扩增技术及在传染性疾病检测中的应用

介绍环介导等温扩增技术的的基本原理、特点及在传染性疾病中检测和诊断的应用。环介导等温扩增技术具有简单、快速、特异性强和扩增效率高等的特点,目前可检测HBV、流感病毒、疱疹病毒、水痘_带状疱疹病毒、腮腺炎病毒、麻疹病毒、腺病毒、SARS病毒、呼吸道合胞病毒、西尼罗病毒、结核分枝杆菌、痢疾志贺菌、大肠埃希菌、螺旋体、肺炎链球菌和耶氏菌等。