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91.
The natural 15N abundances (δ15N values) were measured for nitrate and free and bound amino acids from the leaves of field-grown spinach (Spinacia oleracea L.) and komatsuna (Brassica campestris L.), as well as ureides and free and bound amino acids in the leaves and roots of hydroponically grown soybean (Glycine max L.) totally depending on dinitrogen. Nitrate from the spinach and komatsuna leaves and ureides from leaves and roots of soybean showed higher δ15N values than the total tissue N and N in free or bound amino acid fractions. The δ15N values of individual free and bound amino acids, determined by GC/C/MS using their acetylpropyl derivatives, were similar in leaf tissues except for proline but varied in soybean root tissues. The order of 15N enrichment was similar in the four samples: aspartic acid > glutamic acid > threonine, proline, valine > glycine + alanine +serine, γ-amino butyric acid, and phenylalanine. 相似文献
92.
不同类型水稻土微生物群落结构特征及其影响因素 总被引:5,自引:1,他引:4
选取基于我国土壤地理发生分类的不同类型土壤发育的四种水稻土,利用15N2气体示踪法测定生物固氮速率,采用实时荧光定量PCR(Real-time PCR)技术测定细菌丰度,通过16S rRNA基因高通量测序分析微生物群落组成和多样性。结果表明:变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、绿弯菌门(Chloroflexi)和蓝藻门(Cyanobacteria)是水稻土中优势微生物类群。四种类型土壤发育的水稻土细菌群落结构差异显著(Stress<0.001),群落结构分异(NMDS1)与土壤pH存在极显著正相关关系(P<0.01)。土壤有机碳和碱解氮含量显著影响水稻土中细菌丰度和群落多样性(P<0.01)。红壤发育的水稻土细菌16S rRNA基因拷贝数显著高于其他三种类型水稻土,但OTU数量、Chao1指数和PD指数均低于其他三种类型水稻土。土壤pH对水稻土生物固氮速率有显著影响(P<0.01),紫色土发育的水稻土具有最高的生物固氮速率(3.2±0.7 mg×kg-1×d-1),其中优势类群细鞘丝藻属(Leptolyngbya)可能是生物固氮的主要贡献者。研究结果丰富了对水稻土微生物多样性的认识,为通过调控土壤pH和微生物群落组成来提高稻田生物固氮潜力提供了理论依据。 相似文献
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Md. Milon Sarker Mohammad Belal Hossain Md. Monirul Islam Abu Hena Mustafa Kamal Mohd. Hanafi Idris 《Aquaculture Research》2021,52(1):167-184
Homestead ponds may offer an ideal opportunity in contribution to household income, fish consumption through fish culture. Phytoplankton plays a key indicator in pond productivity. However, the phytoplankton communities in the homestead ponds are nearly undetermined in Bangladesh. This study summarizes the phytoplankton diversity, assemblage and ecological parameters of the homestead ponds in central coastal belt, Bangladesh, based on two seasons. The results revealed that significant differences (p < .05) were found in pH, DO, conductivity, TDS, nitrates, phosphates and sulphates except temperature in monsoon, and transparency in both seasons. A total of 34 phytoplankton genera representatives of five major groups were recorded such as Euglenophyceae (41.5%), Chlorophyceae (30.5%), Cyanophyceae (13%), Dinophyceae (10%) and Bacillariophyceae (5%). A significant difference (p < .05) in phytoplankton abundance was found in both seasons with the highest mean of 186.13 ± 129.34 × 104 cells L?1 in monsoon and the lowest mean of 144.40 ± 107.26 × 104 cells L?1 during winter. The diversity (H'), evenness (J'), richness (d) and dominance (D) ranged from 1.62 to 2.47, 0.52 to 0.97, 0.37 to 1.15 and 0.10 to 0.28 respectively. Based on SIMPER, the most dominant (>10%) genera for dissimilation within stations and seasons were Euglena spp., Phacus spp., Chlorella spp., Strombomonas spp. and Peridinium spp. ANOSIM results stated that 12 genera of phytoplankton were significant contributors based on average dissimilarity. Pearson's correlation coefficient and canonical correspondence analysis (CCA) revealed that phytoplankton abundance, diversity and community were mainly governed by transparency, dissolved oxygen and nitrates. 相似文献
96.
Penny R. Hirsch Lucy M. Gilliam Saran P. Sohi Jennie K. Williams Ian M. Clark Phil J. Murray 《Soil biology & biochemistry》2009,41(9):2021-2024
If soil communities rely on plant-derived carbon, is biodiversity lost when this primary source is removed? Soil microbial and mesofaunal communities at the Rothamsted Highfield site were compared under a mixed grass sward, arable rotation and a section maintained as a bare-fallow for the past 50 years by regular tillage. Organic matter reserves have been degraded and microbial and mesofaunal numbers and mite diversity have declined in this unique bare-fallow site, where fresh carbon inputs have been drastically reduced. However, it supports a species-rich metabolically active bacterial community of similar diversity to that in soil maintained as grass sward. Thus in contrast to soil mesofauna, bacterial diversity (but not abundance) is apparently independent of plant inputs. 相似文献
97.
Giani M.B. Bohm Segundo Urquiaga Gustavo R. Xavier Cesar V. Rombaldi 《Soil biology & biochemistry》2009,41(2):420-422
Over half of the 21 Mha of soybean planted in Brazil is now transgenic glyphosate-resistant (GMRR). A field experiment was carried out to investigate whether the application of glyphosate or imazethapyr to the GMRR variety reduced the input of N2 fixation (BNF). No effects on yield, total N accumulation, nodulation and BNF (δ15N) could be assigned to the genetic modification of the plant. Imazethapyr reduced soybean yield but had no significant effect on BNF. Even though yields were not affected by glyphosate, the significant reduction of nodule mass and BNF to the GMRR suggests that the use of this herbicide could lead to an increased dependence on soil N and consequently an eventual decrease of SOM reserves. 相似文献
98.
以青海省海南州贵德县拉鸡山地区植物群落为研究对象,从海拔3100~3920m,划分为9个海拔梯度,研究了植物生物量及物种丰富度沿海拔梯度的变化特征。结果表明:阳坡地上生物量与物种丰富度呈极显著正相关关系(r=0.564,P<0.01),总生物量与根冠比呈极显著正相关关系(r=0.714,P<0.01);海拔与地上生物量呈极显著负相关关系(r=-0.773,P<0.01),与地下生物量、总生物量、根冠比呈极显著正相关关系(r=0.769、r=0.753、r=0.692,P<0.01)。阴坡植物群落总生物量与地上生物量之间呈显著正相关关系(r=0.495,P<0.05),与地下生物量之间成极显著正相关关系(r=0.960,P<0.01),与海拔呈极显著负相关(r=-0.519,P<0.01);根冠比与地上生物量呈极显著负相关关系(r=-0.631,P<0.01)。地上生物量与海拔呈极显著负相关(r=-0.678,P<0.01)。 相似文献
99.
A natural‐13C‐labeling approach—formerly observed under controlled conditions—was tested in the field to partition total soil CO2 efflux into root respiration, rhizomicrobial respiration, and soil organic matter (SOM) decomposition. Different results were expected in the field due to different climate, site, and microbial properties in contrast to the laboratory. Within this isotopic method, maize was planted on soil with C3‐vegetation history and the total CO2 efflux from soil was subdivided by isotopic mass balance. The C4‐derived C in soil microbial biomass was also determined. Additionally, in a root‐exclusion approach, root‐ and SOM‐derived CO2 were determined by the total CO2 effluxes from maize (Zea mays L.) and bare‐fallow plots. In both approaches, maize‐derived CO2 contributed 22% to 35% to the total CO2 efflux during the growth period, which was comparable to other field studies. In our laboratory study, this CO2 fraction was tripled due to different climate, soil, and sampling conditions. In the natural‐13C‐labeling approach, rhizomicrobial respiration was low compared to other studies, which was related to a low amount of C4‐derived microbial biomass. At the end of the growth period, however, 64% root respiration and 36% rhizomicrobial respiration in relation to total root‐derived CO2 were calculated when considering high isotopic fractionations between SOM, microbial biomass, and CO2. This relationship was closer to the 50% : 50% partitioning described in the literature than without fractionation (23% root respiration, 77% rhizomicrobial respiration). Fractionation processes of 13C must be taken into account when calculating CO2 partitioning in soil. Both methods—natural 13C labeling and root exclusion—showed the same partitioning results when 13C isotopic fractionation during microbial respiration was considered and may therefore be used to separate plant‐ and SOM‐derived CO2 sources. 相似文献
100.