全文获取类型
收费全文 | 494篇 |
免费 | 38篇 |
国内免费 | 125篇 |
学科分类
生物科学 | 657篇 |
出版年
2023年 | 7篇 |
2022年 | 8篇 |
2021年 | 10篇 |
2020年 | 17篇 |
2019年 | 16篇 |
2018年 | 18篇 |
2017年 | 19篇 |
2016年 | 20篇 |
2015年 | 13篇 |
2014年 | 21篇 |
2013年 | 20篇 |
2012年 | 29篇 |
2011年 | 28篇 |
2010年 | 8篇 |
2009年 | 25篇 |
2008年 | 18篇 |
2007年 | 36篇 |
2006年 | 29篇 |
2005年 | 21篇 |
2004年 | 24篇 |
2003年 | 19篇 |
2002年 | 25篇 |
2001年 | 22篇 |
2000年 | 14篇 |
1999年 | 13篇 |
1998年 | 16篇 |
1997年 | 17篇 |
1996年 | 19篇 |
1995年 | 17篇 |
1994年 | 12篇 |
1993年 | 9篇 |
1992年 | 15篇 |
1991年 | 10篇 |
1990年 | 16篇 |
1989年 | 12篇 |
1988年 | 8篇 |
1987年 | 5篇 |
1986年 | 5篇 |
1985年 | 5篇 |
1984年 | 1篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 2篇 |
1979年 | 1篇 |
1977年 | 1篇 |
排序方式: 共有657条查询结果,搜索用时 15 毫秒
61.
First exploration of Nitrobacter diversity in soils by a PCR cloning-sequencing approach targeting functional gene nxrA 总被引:1,自引:0,他引:1
Nitrite oxidoreductase (NXR) is the key enzyme responsible for the oxidation of NO(2)(-) to NO(3)(-) in nitrite-oxidizing bacteria. For the first time a molecular approach for targeting the nxrA gene was developed, encoding the catalytic subunit of the NXR, to study diversity of Nitrobacter-like organisms based on the phylogeny of nxrA gene sequences in soils. NxrA sequences of the Nitrobacter strains analysed (Nitrobacter hamburgensis, Nitrobacter vulgaris, Nitrobacter winogradskyi, Nitrobacter alkalicus) by PCR, cloning and sequencing revealed the occurrence of multiple copies of nxrA genes in these strains. The copy number and similarity varied among strains. The diversity of Nitrobacter-like nxrA sequences was explored in three soils (a French permanent pasture soil, a French fallow soil, and an African savannah soil) using a cloning and sequencing approach. Most nxrA sequences found in these soils (84%) differed from nxrA sequences obtained from Nitrobacter strains. Moreover, the phylogenetic distribution and richness of nxrA-like sequences was extremely variable depending on soil type. This nxrA tool extends the panel of functional genes available for studying bacteria involved in the nitrogen cycle. 相似文献
62.
Reigstad LJ Richter A Daims H Urich T Schwark L Schleper C 《FEMS microbiology ecology》2008,64(2):167-174
Archaea have been detected recently as a major and often dominant component of the microbial communities performing ammonia oxidation in terrestrial and marine environments. In a molecular survey of archaeal ammonia monooxygenase (AMO) genes in terrestrial hot springs of Iceland and Kamchatka, the amoA gene encoding the alpha-subunit of AMO was detected in a total of 14 hot springs out of the 22 investigated. Most of these amoA-positive hot springs had temperatures between 82 and 97 degrees C and pH range between 2.5 and 7. In phylogenetic analyses, these amoA genes formed three independent lineages within the known sequence clusters of marine or soil origin. Furthermore, in situ gross nitrification rates in Icelandic hot springs were estimated by the pool dilution technique directly on site. At temperatures above 80 degrees C, between 56 and 159 mumol NO(3)(-) L(-1) mud per day was produced. Furthermore, addition of ammonium to the hot spring samples before incubation yielded a more than twofold higher potential nitrification rate, indicating that the process was limited by ammonia supply. Our data provide evidence for an active role of archaea in nitrification of hot springs in a wide range of pH values and at a high temperature. 相似文献
63.
64.
65.
Stability and microbial community structure of a partial nitrifying fixed-film bioreactor in long run 总被引:1,自引:0,他引:1
A partial nitrification system was investigated for 471 days under DO varying concentrations for assessing its stability and population dynamics. Within 130 days of operation at feed DO concentration of 1.0 ± 0.1 mg/L, more than 85% of nitrite was accumulated. Efficiency deteriorated when the feed DO concentration was increased to 4.2 ± 0.3 mg/L. Nitrite accumulation could not be re-established on decreasing feed DO to 1.0 ± 0.1 mg/L. Even at DO concentration of <0.05 mg/L, nitrate production was observed; a condition termed as anoxic nitrification. NOB was detected in the biomass even under this condition by Fluorescence in-situ hybridization (FISH) analysis. Through 16S rRNA gene sequencing a major fraction of unknown bacterial sequences closely resembling haloalkalophilic bacteria of marine origin were detected. The study indicated that these bacterial species might play a role in anoxic nitrification and that NOB could survive extreme low DO condition. 相似文献
66.
67.
Ammonia removal from a waste air stream using a biotrickling filter packed with polyurethane foam through the SND process 总被引:4,自引:0,他引:4
This paper presents the results of a bench-scale biotrickling filter (BTF) on the removal of ammonia gas from a waste stream using a simultaneous nitrification/denitrification (SND) process. It was found that the developed BTF could completely remove 100 ppm ammonia from a waste stream, with an empty bed retention time of 60 s and 98.4% nitrogen removal through the SND process under the tested conditions. It was elucidated that both autotrophic and heterotrophic bacteria were involved in the nitrogen removal trough the SND process in the BTF. Additionally, the elimination capacity of total nitrogen by the BTF increased from 3.5 to 18.4 g N/m3 h with an inlet load of 20.6 g N/m3 h (73.6%). The findings of this study suggest that the BTF can be operated to attain complete ammonia removal through the SND process, thereby making the treatment of ammonia-laden gas streams both short and cost-effective. 相似文献
68.
69.
Dinitrogen oxide production by a mixed culture of nitrifying bacteria during ammonia shock loading and aeration failure 总被引:2,自引:0,他引:2
Burgess JE Colliver BB Stuetz RM Stephenson T 《Journal of industrial microbiology & biotechnology》2002,29(6):309-313
A number of experiments was conducted in order to establish if N2O in the exhaust gas from an aerobic consortium of nitrifiers could be used as an indicator for monitoring the nitrification
process. Laboratory-scale experiments with an activated sludge system showed a strong correlation between ammonia shock loads
and both the concentration of N2O and the rate of increase of N2O in the exhaust gas for shock loads less than 1.60 mg ammonical nitrogen (NH3-N) per g total suspended solids (TSS). For greater ammonia shock loads, correlation was found between build-up of nitrite
in the aeration tank and the concentration of N2O in the exhaust gas from the tank. When subjecting the system to aeration failure, a similar pattern was seen, with a correlation
between nitrite build-up in the aeration tank and increases in the concentration of N2O in the exhaust gas. The results from this work suggest that the changes in N2O concentration in the exhaust gas from a nitrifying process may be a useful parameter for monitoring such processes.
Received 15 October 2001/ Accepted in revised form 05 June 2002 相似文献
70.
Nitrogen Dynamics in Ice Storm-Damaged Forest Ecosystems: Implications for Nitrogen Limitation Theory 总被引:3,自引:1,他引:2
Benjamin Z.?HoultonEmail author Charles T.?Driscoll Timothy J.?Fahey Gene E.?Likens Peter M.?Groffman Emily S.?Bernhardt Donald C.?Buso 《Ecosystems》2003,6(5):431-443
Despite the widely recognized importance of disturbance in accelerating the loss of elements from land, there have been few empirical studies of the effects of natural disturbances on nitrogen (N) dynamics in forest ecosystems. We were provided the unusual opportunity for such study, partly because the intensively monitored watersheds at the Hubbard Brook Experimental Forest (HBEF), New Hampshire, experienced severe canopy damage following an ice storm. Here we report the effects of this disturbance on internal N cycling and loss for watershed 1 (W1) and watershed 6 (W6) at the HBEF and patterns of N loss from nine other severely damaged watersheds across the southern White Mountains. This approach allowed us to test one component of N limitation theory, which suggests that N losses accompanying natural disturbances can lead to the maintenance of N limitation in temperate zone forest ecosystems. Prior to the ice storm, fluxes of nitrate (NO3
–) at the base of W1 and W6 were similar and were much lower than N inputs in atmospheric deposition. Following the ice storm, drainage water NO3
– concentrations increased to levels that were seven to ten times greater than predisturbance values. We observed no significant differences in N mineralization, nitrification, or denitrification between damaged and undamaged areas in the HBEF watersheds, however. This result suggests that elevated NO3
- concentrations were not necessarily due to accelerated rates of N cycling by soil microbes but likely resulted from decreased plant uptake of NO3
-. At the regional scale, we observed high variability in the magnitude of NO3
- losses: while six of the surveyed watersheds showed accelerated rates of NO3
– loss, three did not. Moreover, in contrast to the strong linear relationship between NO3
– loss and crown damage within HBEF watersheds [r
2: (W1 = 0.91, W6 = 0.85)], stream water NO3
– concentrations were weakly related to crown damage (r
2 = 0.17) across our regional sites. The efflux of NO3
– associated with the ice storm was slightly higher than values reported for soil freezing and insect defoliation episodes, but was approximately two to ten times lower than NO3
– fluxes associated with forest harvesting. Because over one half of the entire years worth of N deposition was lost following the ice storm, we conclude that catastrophic disturbances contribute synergistically to the maintenance of N limitation and widely observed delays of N saturation in northern, temperate zone forest ecosystems.
Present address: Department of Ecology and Evolutionary Biology, Princeton University, Guyot Hall, Princeton, New Jersey 08544, USA. 相似文献