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41.
High rates of deforestation in the Brazilian Amazon have the potential to alter the storage and cycling of carbon (C) and nitrogen (N) across this region. To investigate the impacts of deforestation, we quantified total aboveground biomass (TAGB), aboveground and soil pools of C and N, and soil N availability along a land-use gradient in Rondônia, Brazil, that included standing primary forest, slashed primary and secondary forest, shifting cultivation, and pasture sites. TAGB decreased substantially with increasing land use, ranging from 311 and 399 Mg ha–1 (primary forests) to 63 Mg ha–1 (pasture). Aboveground C and N pools declined in patterns and magnitudes similar to those of TAGB. Unlike aboveground pools, soil C and N concentrations and pools did not show consistent declines in response to land use. Instead, C and N concentrations were strongly related to percent clay content of soils. Concentrations of NO3-N and NH4-N generally increased in soils following slash-and-burn events along the land-use gradient and decreased with increasing land use. Increasing land use resulted in marked declines in NO3-N pools relative to NH4-N pools. Rates of net nitrification and N-mineralization were also generally higher in postfire treatments relative to prefire treatments along the land-use gradient and declined with increasing land use. Results demonstrate the linked responses of aboveground C and N pools and soil N availability to land use in the Brazilian Amazon; steady reductions in aboveground pools along the land-use gradient were accompanied by declines in inorganic soil N pools and transformation rates. 相似文献
42.
The nitrifying bacteria in activated sludge and biofilms consisting of the bacteria immobilized on polypropylene packing were subjected to an electric current via two electrodes. In activated sludge, the metabolism of nitrifying bacteria was inhibited when the applied current was over 2.5 A m–2, whilst in biofilms, inhibition began when the current reached 5 A m–2. At 15 A m–2, the nitrification rate of NH4
+-N in a biofilm with a bacterial density of 1.62 g total solids, dry wt m–2 decreased to about 80% of its initial value. Ninety-two % of the initial biomass on the packing was retained after 36 h. 相似文献
43.
Simultaneous nitrification and denitrification using a mixed methanotrophic culture was investigated. When both NO3
–-N (108 mg l–1) and NH3-N (59 mg l–1) were added into batch reactors, nitrate removal was complete within 10 h at the rate of 47 mg NO3
–-N g VSS–1 day–1 when dissolved oxygen (DO) concentration was maintained at 2 mg DO l–1. Ammonia removal started simultaneously with nitrate removal at a slower rate of 14 NH3-N g VSS–1 day–1. No significant accumulation of nitrite or nitrate during ammonia utilization suggested the occurrence of simultaneous nitrification and denitrification. 相似文献
44.
New pathways for ammonia conversion in soil and aquatic systems 总被引:9,自引:0,他引:9
Ammonia conversion processes are essential for most soil and aquatic systems. Under natural conditions, the many possible reactions are difficult to analyze. For example, nitrification and denitrification have long been regarded as separate phenomena performed by different groups of bacteria in segregated areas of soils, sediments or aquatic systems sequentially in time. It has now been established that strict segregation in place and time of the two processes is not necessary and that both denitrifiers and nitrifiers have versatile metabolisms. However, the rates described for aerobic denitrifiers are very low compared to the rates observed under anoxic conditions. Also the rates of nitrifier denitrification are quite low, indicating that these conversions may not play an important role under natural conditions. In addition, these processes often result in the emission of quite large amounts of undesirable products, NO and N2O. Heterotrophic nitrification might be of relevance for systems, that contain a high carbon to nitrogen ratio. Recently, a novel process (Anammox) has been discovered in which ammonium serves as the electron donor for denitrification of nitrite into dinitrogen gas. 15N labeling studies showed that hydrazine and hydroxylamine were important intermediates in this process. Enrichment cultures on ammonium, nitrite and bicarbonate resulted in the dominance of one morphotypical microorganism. The growth rate of the cultures is extremely low (doubling time 11 days), but the affinity for ammonium and nitrite and the conversion rates (9.2 10–4 mol kg–1 s–1) are quite high. Some of the reported high nitrogen losses in soil and aquatic systems might be attributed to anaerobic ammonium oxidation. In addition, this conversion offers new opportunities for nitrogen removal, when it is combined with recently developed processes for partial nitrification. 相似文献
45.
Ironhill, near Liphook, UK, was the site of a forest fumigation experiment. Nitrogen cycling within the humoferric podzol soil was a component of the study into the impacts of sulphur dioxide and ozone on coniferous trees. Variation in total soil N and N mineralisation was too great to determine impacts from the fumigant gases. Differences in the nitrogen mineralisation potential of the soils were unrelated to the initial levels of mineral or total N, or to pH. Mineralisation potential was affected by temperature and a Q10 of approximately 3 was demonstrated. Mineralisation potential was reduced in very dry soils, but the wetting of these dry soils did not result in enhanced mineralisation, relative to fresh samples of equivalent moisture content. Nitrification potential was detected in this forest soil of pH 3 (in 0.01 m CaCl2).The soil N data and those from the analysis of N within vegetation were used to prepare N budgets for the second and third seasons' growth of a mixed conifer forest; by the third year, N appeared to limit tree growth.The relative magnitude of proton fluxes from plant growth, nitrification and atmospheric inputs was estimated. Acidity generated from the balance of cations and anions in plant uptake, and soil N transformations was estimated to be comparable to that from `acid rain'. This comparison was based on only parts of the N cycle because they may occur remotely, in time or space, from other transformations of N. The comparison is valid, therefore, at the scale of individual trees or small-scale experimental plots, but at forest scale, wet and dry deposition were predicted to be the more significant for ecosystem acidification. 相似文献
46.
Magid Jakob Henriksen Ole Thorup-Kristensen Kristian Mueller Torsten 《Plant and Soil》2001,228(1):73-82
We examined the decomposition of Medicago lupulina, Melilotus alba and Poa pratensis at 3, 9, and 25 °C during 4 weeks. There was a strong temperature effect on the rate of CO2 evolution, and thus the extent of energy exhaustion from the added substrates. However, there was no concomitant retardation of N mineralisation at low temperatures. In the analysis of variance of mineralized N the residue type gave a 10 times larger contribution to the regression than the temperature (T), whereas for CO2 evolution residue type and temperature were equally important contributors. This indicates that although the temperature has a statistically significant effect on N-mineralisation it is substantially less than compared with the effect on carbon mineralisation in the materials examined. The retardation of carbon mineralisation was least strong in Melilotus alba that had a relatively low cellulose content, and a higher content of low molecular compounds. Though more research will be necessary to consolidate and explain this phenomena, it is likely that an important factor is a decrease in the bioavailability of C-rich polymers at low temperatures, and thus a preferential utilization of N-rich low molecular substances. Nitrification was not effectively deterred at 3 °C. Thus, in terms of management, it is pertinent to reconsider the timing of green manure and catch crop incorporation in cool temperate climate regions, since the rapid release of nitrogen, coupled with the relatively undeterred nitrification may result in a high N leaching risk by early incorporation, but a low risk for N immobilization at late incorporation, if N rich residues are used. 相似文献
47.
Tracing the Sources of Exported Nitrate in the Turkey Lakes Watershed Using 15N/14N and 18O/16O isotopic ratios 总被引:3,自引:0,他引:3
John Spoelstra Sherry L. Schiff Richard J. Elgood Ray G. Semkin Dean S. Jeffries 《Ecosystems》2001,4(6):536-544
Nitrate produced by bacterially mediated nitrification in soils is isotopically distinct from atmospheric nitrate in precipitation.
15N/14N and 18O/16O isotopic ratios of nitrate can therefore be used to distinguish between these two sources of nitrate in surface waters and
groundwaters. Two forested catchments in the Turkey Lakes Watershed (TLW) near Sault Ste. Marie, Ontario, Canada were studied
to determine the relative contributions of atmospheric and microbial nitrate to nitrate export. The TLW is reasonably undisturbed
and receives a moderate amount of inorganic nitrogen bulk deposition (8.7 kg N · ha−1· yr−1) yet it exhibits unusually low inorganic nitrogen retention (average = 65% of deposition). The measured isotopic ratios for
nitrate in precipitation ranged from +35 to +59‰ (VSMOW) for δ18O and −4 to +0.8‰ (AIR) for δ15N. Nitrate produced from nitrification at the TLW is expected to have an average isotope value of approximately −1.0‰ for
δ18O and a value of about 0 to +6‰ for δ15N, thus, the isotopic separation between atmospheric and soil sources of nitrate is substantial. Nitrate produced by nitrification
of ammonium appears to be the dominant source of the nitrate exported in both catchments, even during the snowmelt period.
These whole catchment results are consistent with the results of small but intensive plot scale studies that have shown that
the majority of the nitrate leached from these catchments is microbial in origin. The isotopic composition of stream nitrate
provides information about N-cycling in the forested upland and riparian zones on a whole catchment basis.
Received 5 October 1999; accepted 18 August 2000 相似文献
48.
氨氧化由氨氧化细菌(AOB)和氨氧化古菌(AOA)共同执行,是土壤硝化过程的第一步和限速步骤。放牧过程中,动物啃食、排泄和践踏等行为将影响土壤氨氧化微生物群落,但目前关于不同类型放牧对湿地氨氧化微生物群落结构及其多样性的影响尚不清楚。利用Illumina Mise高通量测序技术,对比研究牦牛放牧和藏香猪放养两种放牧类型对泥炭沼泽土壤氨氧化微生物群落结构及其多样性的影响。结果表明,牦牛放牧显著增加土壤容重,显著降低土壤pH、TN、TOC、NH~+_4-N和NO~-_3-N含量;藏香猪放养显著增加土壤NO~-_3-N含量和硝化潜势(PNR)。牦牛放牧显著降低土壤AOA的丰富度和AOB的α多样性,藏香猪放养降低土壤AOA的α多样性和AOB的丰富度。放牧显著降低泉古菌门(Crenarchaeota)的相对丰度。AOA的α多样性与土壤NO~-_3-N含量和PNR呈显著负相关。AOB的α多样性与pH、TOC、TN和NH~+_4-N含量呈显著正相关。放牧影响下土壤pH、TN和NO~-_3-N含量的变化是影响AOA群落结构的主要因素。藏香猪放养对AOA和AOB群落的影响更显著,由放牧引起的土壤环境条件的变化是导致氨氧化微生物群落发生改变的重要因素。 相似文献
49.
为了获得异养硝化-好氧反硝化菌株,从养殖池塘污泥中分离筛选到一株具有异养硝化-好氧反硝化能力的酵母菌,命名为DW-1。经形态学观察和26S rDNA序列分析后鉴定为皱褶念珠菌DW-1(Diutina rugosa DW-1)。以氨氮为唯一氮源,初步探讨了碳源、C/N、初始pH值、培养温度、摇床转速对菌株DW-1除氮性能的影响。结果表明,在以乙酸钠为唯一碳源,C/N为25,pH为6.0、适宜培养温度为32℃、转速为170 r/min的条件下,菌株DW-1氨氮降解率和总氮去除率分别为94.94%、48.69%,而整个过程中亚硝氮积累量仅为0.067 mg/L。皱褶念珠菌DW-1的异养硝化-好氧反硝化特性表明其在降解含氮废水方面具有良好的应用前景。 相似文献
50.
亚硝酸盐是氮循环过程的中间产物,其积累超过一定量则会抑制微生物的生长与代谢,也会给人与水生生物带来健康风险。而在高氮污水生物脱氮工艺中,持续维持亚硝酸盐的积累能实现短程硝化过程,降低生物脱氮的能耗进而降低运营成本。本文综述了在水环境中亚硝酸盐积累的生物过程与积累原因,并对影响亚硝酸盐积累的因素进行了总结,旨在为提高污水处理过程中氮的去除效率,降低运营成本,减少排放污水及自然水体中亚硝态氮含量提供参考。 相似文献