电针对佐剂性关节炎大鼠关节滑膜细胞内TAK1表达的影响

目的：观察电针足三里、关元穴对佐剂性关节炎（AIA）大鼠踝关节滑膜细胞内转化生长因子β激活激酶1（TAK1）表达的影响,探索电针对类风湿性关节炎（RA）可能的干预机制。方法40只SD大鼠随机分为空白组、模型组、甲氨蝶呤（MTX）组、电针组,每组10只。采用Fueund 氏完全佐剂(FCA)法造模。电针组予以电针大鼠关元穴、双侧足三里穴,MTX组予以0.35 mg/kg剂量的MTX灌胃治疗,空白组与模型组每日予以特制大鼠固定器固定后松绑放回笼内饲养。实验期间每3天1次测量大鼠体质量、足爪容积,并对关节炎指数进行评分;采用放射免疫法检测TNF-α、用Western Blot 检测TAK1、NF-κB蛋白表达。实验结束后比较各组大鼠踝关节滑膜滑膜组织中TNF-α,滑膜细胞内TAK1,胞核内NF-κB表达水平。结果与空白组比较,模型组大鼠体质量增长明显缓慢、足趾肿胀明显、关节炎指数明显升高（P<0.01）,滑膜组织内TNF-α显著升高、滑膜细胞内TAK1含量显著升高、胞核内NF-κB表达显著升高（P<0.01）;与模型组比较,电针组大鼠体质量增长较快、足趾肿胀较轻、关节炎指数较低（P<0.05）,滑膜组织中 TNF-α低于模型组、滑膜细胞内 TAK1、NF-κB 表达较低(P<0.05)。结论电针AIA大鼠足三里、关元等穴能够有效抗炎,其干预机制可能与抑制关节滑膜细胞内TAK1的表达,良性调控NF-κB这一炎症信号通路有关。     

艾叶浴足剂治疗小儿脾虚泻60例

目的:观察艾叶浴足剂治疗小儿脾虚泻的疗效。方法:120例均为中医科就诊患者,随机分成两组,各60例。治疗组在口服白术散、健脾散的基础上,每日1剂温泡双足,同时按摩涌泉、三阴交、足三里等穴。时间为20min,6天为1个疗程。对照组单纯口服白术散、健脾散。结果:治疗组总有效率95%;对照组总有效率为65%(P﹤0.05)。应用艾叶浴足剂的治疗组明显优于对照组。结论:艾叶浴足剂治疗小儿脾虚泻疗效好。     

浅谈足三里穴保健灸

从足三里穴保健灸源流、功效及用途、定位及取穴法、施灸方法、现代研究等方面进行论述,指出操作不便之处,展望未来发展前景。     

电针对心肌缺血模型大鼠孤束核c-fos表达及心电图ST_(Ⅱ)的影响

目的:观察针刺"内关""足三里"对心肌缺血模型大鼠孤束核(nucleus of the solitary tract,NTS)c-fos表达及心电图STⅡ的影响,阐明延髓初级中枢在"内关""足三里"穴共同调节心功能中的作用。方法:将36只雄性SD大鼠随机分为生理盐水组、模型组、足三里组、内关组、偏历组及合阳组,每组6只,腹腔注射异丙肾上腺素造成心肌缺血模型。电针治疗20 min,采用抗Fos蛋白免疫组织化学技术观察大鼠NTS内c-fos表达和电生理技术观察大鼠心电图STⅡ的变化。结果:心肌缺血模型大鼠NTS内c-fos的表达明显增加(P<0.01),而足三里组、内关组与模型组比较,NTS内c-fos阳性细胞数均明显减少(P<0.01);足三里组、内关组NTS内c-fos阳性细胞数显著低于偏历组、合阳组(P<0.01)。足三里组、内关组大鼠缺血性心电图STⅡ电位值显著降低(P<0.01),其电位降低值大于偏历组、合阳组(P<0.01)。结论:NTS是针刺内关、足三里共同调节心功能的整合中枢之一。     

足三里穴注射斯奇康对脑卒中后肺部感染的老年患者免疫功能的影响

目的：观察穴注射斯奇康对脑卒中后肺部感染老年患者免疫功能的影响。方法：将符合纳入标准的60例患者随机分治疗组、对照1组和对照2组。3组患者均给予常规脱水、降颅压、吸氧、营养支持、化痰和抗感染治疗，在此基础上，对照2组加用斯奇康注射液肌肉注射；治疗组加用斯奇康注射液足三里穴注射。观察3组患者治疗前后免疫功能指标的变化和格拉斯哥昏迷评分。结果：治疗组免疫功能指标的改善情况和格拉斯哥昏迷评分均明显优于对照组，差异具有统计学意义（P〈0．05）。结论：足三里穴注射斯奇康能提高脑卒中后肺部感染的老年患者的免疫功能。     

艾灸治疗高脂血症临床观察

目的观察艾灸丰隆、足三里穴治疗高脂血症的临床疗效。方法将66例患者随机分为治疗组34例,采用艾灸丰隆、足三里穴治疗,对照组32例,采用普伐他汀片口服治疗,并检测两组患者治疗前后总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白-胆固醇（LDL-C）、高密度脂蛋白-胆固醇（HDL-C）的变化。结果两组在调节TC、TG及总有效率方面比较差异无统计学意义（P〉0.05）。结论艾灸丰隆、足三里穴对血脂有较好的良性调节作用。     

吴茱萸热熨联合针刺足三里对胃癌术后FTS的疗效观察

目的探讨中西医结合干预对胃癌术后快速康复(FTS)的疗效观察。方法 55例行胃癌根治术的患者,分为常规治疗组(FTS组,n=30)及中西医结合快速康复组(FTS+中西医结合组,n=25)。从术后肛门第一次排气排便、拔除胃管、开始进食时间、住院时间、住院费用、术后并发症及C-反应蛋白方面进行评估。结果中西医结合快速康复组在术后肛门第一次排气排便、拔除胃管、开始进食时间、住院时间、住院费用及术后并发症方面较A组有显著差异(P0.05)。结论中西医结合干预对胃癌术后患者可促进康复、缩小住院时间、减少住院费用及术后并发症。     

Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease

OBJECTIVE: Infl ammation and lung function decline are the main pathophysiological features of chronic obstructive pulmonary disease(COPD). Acupuncture can improve lung function in patients with COPD, but the underlying mechanisms are not well understood. Orexins(OXs), which are found in peripheral plasma, are neuropeptides that regulate respiration and their levels are related to COPD. Therefore, we hypothesized that acupuncture might alter OXs, reduce lung infl ammation and improve lung function in COPD.METHODS: COPD was induced in rats by exposure to cigarette smoke for 8 weeks and injecting with lipopolysaccharide twice. Electroacupuncture(EA) was performed at Feishu(BL13) and Zusanli(ST36) for 30 min/d for 2 weeks. Rat lung function and morphology were assessed after EA. The levels of tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) in bronchoalveolar lavage fl uid(BALF) and orexin A and B levels in the lung tissue were detected by enzyme-linked immunosorbent assay. OX receptor mRNA levels and immunopositive cells were assessed with real-time polymerase chain reaction and immunohistochemical methods, respectively. The relationships among lung function, cell factors, and OX levels were analyzed by Pearson correlation analyses.RESULTS: Compared with the control group, lung function was signifi cantly decreased in the rats with COPD(P0.05). There were obvious increases in TNF-α and IL-1β levels in BALF(P0.05 and P0.01, respectively), orexin A level in lung tissue(P0.01; but not orexin B) and mRNA expressions of OX 1 type receptor(OXR1) and OX 2 type receptor(OXR2) in lung tissue(P0.05 and P0.01, respectively); the integrative optical densities(IODs) of both receptors were greater in the COPD group(P0.05). For rats with COPD subjected to EA, lung function was improved(P0.05). There were notable decreases in TNF-α and IL-1β levels(P0.05 and 0.01, respectively) in BALF. Orexin A, but not orexin B, levels in lung tissue also decreased(P0.01), as did mRNA expression of OX1 R and OX2 R in lung tissue(P0.05 and P0.01, respectively). Receptor IODs were also reduced after EA treatment(P0.05). Furthermore, orexin A levels and ratio of forced expiratory volume in 0.3 s to forced vital capacity were strongly negatively correlated(P0.01), and orexin A was positively correlated with TNF-α and IL-1β(P0.001 and P0.05, respectively).CONCLUSION: EA at Zusanli and Feishu improved lung function of rats with COPD and had an anti-infl ammatory effect, which may be related to down-regulation of OXA and its receptors.     

穴位按摩对人胃电图的影响

以体表胃电图为指标，对２１位健康人和各类胃病患者进行试验，测试按摩穴位对胃电图的影响，从而探讨按摩穴位对胃运动功能的作用。结果显示按摩右胃俞、右足三里对胃窦基本电节律（ＢＥＲ）的幅值有兴奋和抑制两种效应，即呈良性“双向性”调整作用。按摩非经非穴对胃窦ＢＥＲ的幅值也有一些作用，但其影响较按摩穴位者为弱，提示穴位具有相对特异性。     

艾灸预处理对应激性溃疡大鼠胃黏膜HSP70蛋白及mRNA表达的影响

目的:观察艾灸足三里和梁门穴预处理对应激性溃疡大鼠胃黏膜热休克蛋白70(HSP70)及其基因表达的影响,探讨艾灸足阳明经穴保护胃黏膜的作用机制.方法:将60只大鼠完全随机分为空白组(A)、模型组(B)、艾灸足三里等穴组(C)和对照组(D)4组,采用水浸-束缚应激法(WRS)制备应激性溃疡模型.按Guth法计算胃黏膜损伤指数(UI),用免疫组织化学法、逆转录聚合酶链式反应(RT-PCR)法和放射免疫法分别检测处理后大鼠胃黏膜HSP70,HSP70mRNA的表达和内皮素(ET)、前列腺素E2(PGE2)的含量.结果:SU大鼠胃黏膜损伤指数B组与A、C组(P=0.000,P=0.001),D组与A、C组(P=0.001)有显著性差异,艾灸足三里等穴可使SU大鼠胃黏膜损伤指数明显下降.胃黏膜PGE2含量A组与B、D组比较差异显著(P=0.011,P=0.028),C组与B、D组比较差异显著(P=0.020,P=0.048),经艾灸预处理的大鼠胃黏膜PGE2含量均有不同程度升高.ET含量B组与A组之间有显著差异(P=0.040),经艾灸预处理的大鼠ET含量下降显著,B组与C组相比差异显著(P=0.020).造模后胃黏膜的HSP70蛋白和mRNA表达均有不同程度的增强,B组与A组相比有显著性差异(P=0.039,P=0.008);经艾灸预处理后HSP70蛋白和mRNA显著增强,C组与B、D组比较有统计学意义(蛋白:P=0.003,P=0.035;mRNA:P=0.000,P=0.001).结论:艾灸足三里、梁门穴能通过增强HSP70的蛋白和基因表达,达到对胃黏膜的保护作用,并有一定的穴位特异性.