口腔扁平苔藓病损区白细胞介素12p40和干扰素-γ的表达及意义

目的 探讨白细胞介素12p40(IL-12p40)及干扰素-γ(IFN-γ)在口腔扁平苔藓(OLP)病损形成及发展中的意义.方法 正常口腔黏膜组织11例,OLP组织43例,采用免疫组织化学Envision二步法检测IL-12p40和IFN-γ的表达情况,分析其与OLP患者临床病理特征的关系.结果 1)OLP组IL-12...     

Intraoral myxoid nerve sheath tumour

A case of an intraoral myxoid nerve sheath tumour of the dorsum of the tongue in a 73-year-old Caucasian male is reported. This case describes the oldest patient with this pathology to date. Immunoperoxidase staining for neuron-specific enolase (NSE) and epithelial membrane antigen (EMA) expression demonstrated the perineural origin of the lesion.     

口腔粘膜良性淋巴组织增生症的临床病理和免疫组化分析

目的明确口腔粘膜良性淋巴组织增生症（BLOM）的临床病理特征及其本质。方法收集分析6例BLOM的临床病理资料，并进行文献复习；对该组病例的组织标本进行免疫组化研究。结果发生在唇部和其它口腔解剖部位的口腔粘膜良性淋巴组织增生症的临床表现和诊断各有其特点；B淋巴细胞是淋巴滤泡和浸润炎症细胞的主要细胞万分。结论BLOM分为唇型和非唇型两种临床类型；BLOM是一种B淋巴细胞介导的增殖性局部体液免疫反应疾病。     

年轻恒牙牙根发育过程中牙髓中Ⅲ型胶原的表达

目的：研究牙根发育不同阶段的人年轻恒牙牙髓中Ⅲ型胶原的表达变化：方法：根据牙根的发育情况，分为牙根刚开始发育、牙根发育中和根尖闭合共3组：采用SP免疫组化法。对各组牙髓标本的石蜡切片进行Ⅲ型胶原的免疫组化染色：结果：图像分析显示、3个组的冠髓中心染色强度之间有非常显著性差异，以牙根刚开始发育时染色最强，根尖闭合时染色最弱：在牙根刚开始发育组、牙髓中心区染色强于牙髓外层。结论：随着年轻恒牙牙根的逐渐发育，Ⅲ型胶原在牙髓中的表达逐渐减弱，因而可能参与牙根的发育。     

核心结合因子a1在小鼠牙齿发育过程中的表达

目的：观察Cbfal蛋白在小鼠牙胚发育过程中的表达情况。方法：用自制的Cbfal多克隆抗体，采用免疫组化染色观察其时空表达特性。结果：Cbfal蛋白在帽状期牙胚中表达较广泛，在钟状早、中期牙胚表达于内釉细胞、成釉细胞以及紧靠成牙本质细胞层的牙乳头细胞，而在钟状晚期，Cbfal在牙乳头表达为阴性，成牙本质细胞层弱阳性，成釉细胞为阳性或强阳性。结论：Cbfal可能在小鼠牙胚发育，尤其是在成牙本质细胞和成釉细胞分化过程中具有重要作用。     

基质金属蛋白酶-7和c-myc在口腔鳞状细胞癌中的表达和意义

目的研究基质金属蛋白酶-7(MMP-7)与c-myc在口腔鳞状细胞癌组织中的表达及其意义。方法应用免疫组化EliVisionTM法分别检测MMP-7和c-myc在49例口腔鳞状细胞癌和5例正常口腔黏膜上皮中的表达情况。结果1)MMP-7和c-myc在口腔鳞状细胞癌组织中的阳性表达率分别为83.7%和77.6%。2)在不同病理分级、不同临床分期和有无淋巴转移的口腔鳞状细胞癌组织中MMP-7阳性表达率的差异有统计学意义(P<0.05)。3)在不同病理分级和有无淋巴转移的口腔鳞状细胞癌组织中c-myc阳性表达率的差异亦有统计学意义(P<0.05)。4)口腔鳞状细胞癌组织中MMP-7和c-myc的表达呈正相关(P<0.05)。结论MMP-7和c-myc在口腔鳞状细胞癌组织中均有高表达,在口腔鳞状细胞癌的发病过程中,其高表达具有协同效应,共同促进口腔鳞状细胞癌的发生和发展。     

Immunohistochemical analysis of CD1a-labeled Langerhans cells in human dental periapical inflammatory lesions--correlation with inflammatory cells and epithelial cells

OBJECTIVE: Distribution and density of CD1a-labeled Langerhans cells (LCs) were examined in human dental periapical inflammatory lesions, and compared with inflammatory cell infiltration or epithelial cell proliferation. MATERIALS AND METHODS: Eighty three periapical lesions (26 apical granulomas (AGs), 8 epitheliated granulomas (EGs), 34 radicular cysts (RCs), 15 residual radicular cysts (RRCs)) were collected. As control, specimens of periodontal ligaments including Malassez epithelial rests were curetted from 21 teeth. LC densities were measured and various degrees of inflammatory cell infiltration were examined immunohistochemically. Labeling indices for the cellular proliferation markers Ki-67 antigen and DNA topoisomerase II alpha were calculated in the epithelial components. RESULTS: LCs were found in all periapical lesions but not in Malassez epithelial rests. LCs were more abundant in epithelial components than in subepithelial layers. Intraepithelial LCs were more frequent in RCs than in RRCs, whereas subepithelial LCs were less frequent in RRCs than in AGs and EGs. T lymphocytes consistently outnumbered macrophages, plasma cells and B lymphocytes. The range of the CD4/CD8-positive cell ratio differed according to the lesions. Increased LC density was associated with the severity of CD3-positive cell infiltration. Ki-67- and Topo II-LI showed various degrees of epithelial immunoreactivity. There was a significant correlation between LC density and proliferative potential of the epithelium in periapical lesions. CONCLUSION: These findings suggested that LCs appeared to be associated with T lymphocyte infiltration and proliferative potential of the epithelial tissue in periapical lesions.     

Immunoexpression of extracellular matrix proteins in human salivary gland development

Immunoexpression of the extracellular matrix (ECM) proteins laminin, fibronectin, tenascin and types I, III and IV collagen was analyzed in the major and minor salivary glands of seven human fetuses at different gestational ages. The results showed the presence and localization of laminin, collagen IV and fibronectin around glandular structures at all stages of development. Tenascin was only detectable around excretory ducts. In the earliest stages of development, type I and type III collagen were presented as fine fibers delineating the glandular structures and delimiting the extension of the future lobule. As glandular development proceeded, the lobule was gradually filled with collagens and glandular tissue.     

Expression of CD44s, CD44v5, CD44v6 and CD44v7-8 in betel quid chewing-associated oral premalignant lesions and squamous cell carcinomas in Taiwan

Aberrant expression of the cell surface adhesion molecule CD44 and its variant forms has been shown to be associated with the invasive and metastatic potential of cancer cells, and with poor prognosis in several types of cancers. Expression of CD44 standard (CD44s) and variant (CD44v) forms in oral squamous cell carcinoma (SCC), epithelial dysplasia (ED), epithelial hyperkeratosis (EH) and normal buccal mucosa (NBM) have been examined using antibodies to CD44s, CD44v5, CD44v6 and CD44v7-8. Positive CD44s, CD44v5, CD44v6 and CD44v7-8 staining was detected in all the specimens from NBM, EH and ED. Positive staining of CD44s, CD44v5, CD44v6 or CD44v7-8 was detected in 55 (88.7%), 48 (77.4%), 59 (95.2%) and 22 (35.5%) of the 62 SCC specimens, respectively. The positive staining of CD44v7-8 in oral SCC was significantly less than that in NBM ( P <0.01). No significant correlation was found between CD44v7-8 expression and daily or total consumption of betel quids or cigarettes by the SCC patients. The 5-year survival rate for patients with CD44v7-8-positive tumours was significantly higher than that for the CD44v7-8-negative group ( P <0.03). These results indicate that loss of CD44v7-8 expression may be a valuable factor for determining prognosis in oral SCC patients.     

Expression of two drug-metabolizing cytochrome P450-enzymes in human salivary glands

Objective:  The oral cavity is constantly lubricated by saliva and even small amounts of xenobiotics and / or their metabolites in the saliva may affect the oral mucosa. Our aim was therefore to clarify if xenobiotic metabolizing enzymes CYP1A2 and CYP3A4 are expressed in salivary glands.
Methods:  Formalin-fixed paraffin-embedded specimens from parotid (10), submandibular (7) and labial (10) salivary glands were examined immunohistochemically and by in situ hybridization for expression of CYP1A2 and CYP3A4 protein and mRNA.
Results:  CYP1A2 and CYP3A4 protein and mRNA were detected in ductal and seromucous / serous acinar cells in all gland types although to a varying degree and intensity. Mucous acinar cells were positive to a lesser extent.
Conclusion:  The results indicate a xenobiotic metabolizing capability of salivary glands. This may have implications for development of oral mucosal disease as a result of mucosal exposure to metabolites originating from internal sources (blood) as well as from saliva.