黄蘑多糖的辐射防护作用及其机理的初步探讨

本文研究了黄蘑多糖的辐射防护作用及其对受照小鼠肝脏组织中LPO含量及SOD、GSH-PX和CAT活性的影响。结果表明,黄蘑多糖可明显提高受致死剂量照射小鼠的30 d存活率(P <0.05),延长存活天数,保护指数可达1.32,并且,黄蘑多糖的这种辐射防护效果与有效的辐射防护药物人参多糖相似。进一步研究发现,小鼠经8.0Gy X射线照射后72h,黄蘑多糖能明显降低受照小鼠肝脏中LPO含量(P <0.01),且显著增强其SOD、GSH-PX和CAT活性(P <0.05)。提示,黄蘑多糖具有明显的辐射防护作用,且可与人参多糖相比。其作用机理与促进自由基清除,抑制或阻断自由基引发的脂质过氧化反应,提高机体抗氧化能力有关。     

不同敷料组合对烧伤患者供皮区创面微环境的影响

Objective To investigate the effect of dressing materials in various combinations on bum wound microenvironment and healing condition. Methods Two hundred donor sites with wounds of 0.3 mm in depth in 186 burn patients, who needed skingrafting and admitted to our ward were enrolled in study, and they were divided into A ( with dressing composed of aiginate + cotton pad for donor area), B ( with dressing composed of vaseline gauze + cotton pad for donor area) , C (with dressing composed of algi-nate+ foam dressing for donor area), D ( with dressing composed of vaseline gauze + foam dressing for donor area) groups according to random table method. Effect of dressings on wound evaporation and pH value were observed. Bacterial colonization, degree of pain complained by patients after dressing change, and wound healing time in each group were compared. Results One hundred and eighty-four patients complied with the study, while 2 patients were excluded due to untimely falling-off of the dressing. Wound evaporaton in respectively, among them B group showed optimal effect of keeping humidity (P<0.01). Wound pH value in A, B, C, D groups was 7.22±0.06, 7.41±0.03, 7.05±0.03, 7.34±0.06, respectively, among them it was highest in B group. The positive rate of bacteria in D group was highest (22.4%), and lowest in C group (4.0%). Pain was lightest in C group (score was 0.98±0.12), and most serious in B group ( score was 8.14±0.82). The shortest wound healing time was seen in C group (6.7±0.8 d) , and longest in D group (15.6±3.5 d). Conclusions Application of various dressings on similar wounds can pro-duce different wound microenvironment, which is closely related to wound healing time. Compared with pH value, humidity is the more important factor for wound healing.     

不同敷料组合对烧伤患者供皮区创面微环境的影响

Objective To investigate the effect of dressing materials in various combinations on bum wound microenvironment and healing condition. Methods Two hundred donor sites with wounds of 0.3 mm in depth in 186 burn patients, who needed skingrafting and admitted to our ward were enrolled in study, and they were divided into A ( with dressing composed of aiginate + cotton pad for donor area), B ( with dressing composed of vaseline gauze + cotton pad for donor area) , C (with dressing composed of algi-nate+ foam dressing for donor area), D ( with dressing composed of vaseline gauze + foam dressing for donor area) groups according to random table method. Effect of dressings on wound evaporation and pH value were observed. Bacterial colonization, degree of pain complained by patients after dressing change, and wound healing time in each group were compared. Results One hundred and eighty-four patients complied with the study, while 2 patients were excluded due to untimely falling-off of the dressing. Wound evaporaton in respectively, among them B group showed optimal effect of keeping humidity (P<0.01). Wound pH value in A, B, C, D groups was 7.22±0.06, 7.41±0.03, 7.05±0.03, 7.34±0.06, respectively, among them it was highest in B group. The positive rate of bacteria in D group was highest (22.4%), and lowest in C group (4.0%). Pain was lightest in C group (score was 0.98±0.12), and most serious in B group ( score was 8.14±0.82). The shortest wound healing time was seen in C group (6.7±0.8 d) , and longest in D group (15.6±3.5 d). Conclusions Application of various dressings on similar wounds can pro-duce different wound microenvironment, which is closely related to wound healing time. Compared with pH value, humidity is the more important factor for wound healing.     

辅酶Q10在荷瘤小鼠放疗中对机体的保护作用及抑瘤机理

辅酶Ｑ１０在荷瘤小鼠放疗中对机体的保护作用及抑瘤机理冯乐平陈军苏士杰董永兰放疗在杀伤机体肿瘤细胞的同时对正常细胞也产生损害作用，如何能够既有效地杀灭肿瘤细胞，又减少对正常细胞的危害，已成为当今临床放射生物学界亟待解决的问题。辅酶Ｑ１０（简称Ｑ１０）是...     

低水平X射线对荷瘤鼠机体的生物效应

低水平X射线在小鼠肿瘤大剂量放疗中的应用表明,在局部放疗前适当给予低水平X射线预照射,可诱导胸腺细胞自发增殖能力增强,使脾细胞损伤后修复合成能力增加,从而提高了机体的抗肿瘤作用。本文结果为低剂量辐射在临床肿瘤放疗中的应用提供了理论基础。     

新型羟乙基淀粉应用于烧伤休克期液体复苏的疗效及安全性评价

目的评价新型羟乙基淀粉(HES)制剂在烧伤患者休克期液体复苏中的应用疗效及安全性。方法选择笔者单位收治的烧伤休克期需液体复苏患者66例,按表格随机化方法分成HES组(33例)和血浆组(33例)。两组患者伤后48 h内给予的液体中胶体成分仅为HES(130／0．4, 60 g／L)或血浆,伤后3-7 d两组均替换成白蛋白作为胶体成分[5 d共给予白蛋白各(111±4)、(105±5)g],本试验全过程两组所给予的营养支持、抗感染等措施相同。监测患者伤后24 h内的心率、血压、中心静脉压、尿量,并记录伤后第1、2个24 h的胶体输入量、进出量比,于伤后指定时相点检测血清总蛋白、白蛋白、血红蛋白、凝血酶原时间、纤维蛋白原、血小板计数及某些肝、肾功能指标,观察患者有无变态反应发生及出血情况。结果两组患者伤后心率、血压、中心静脉压、尿量及第1、2个24 h的胶体输入量、进出量比差异无统计学意义(P>0．05)．患者伤后1、3、7、14 d的血红蛋白及1、3、14 d的凝血酶原时间、纤维蛋白原、血小板计数组间比较,差异亦无统计学意义(P>0．05);HES组在伤后1、3 d的血清总蛋白和白蛋白各为(31±3)、(30±3)只／L和(20．4±3．6)、(18．4±2．3) g/L,均明显低于血浆组的(45±4)、(39±3)g/L和(24.5±4．3)、(21．3±3．9)g／L(P<0.02);伤后7 d两组血清白蛋白水平较接近(P>0．05),但HES组血清总蛋白(40±4)g／L仍明显低于血浆组的(45±4)g／L(P<0．01)。两组患者肝功能指标变化基本一致,肾功能无异常改变,均未见明显的出血倾向。HES组无变态反应发生,血浆组有4例出现荨麻疹。结论新型HES130／0．4可部分代替血浆用于烧伤患者休克期的液体复苏治疗,但要注意在一定时相点及时补充血浆蛋白。     

黄蘑多糖对小鼠H_(22)腹水肝癌细胞周期的影响

黄蘑属担子菌类,其学名根据子实层上有无囊状体称为侧耳[Pleurotus ostreatus(Jaeq:Fr.)Quei.]和亚侧耳[Hohenbuehelia seiotina(Schard.:Fr.)Sing.],俗称元蘑或黄蘑。主要产于长白山区,为营养丰富、味道鲜美、肉质肥厚的野生食用菌,具有疏风活络、强筋壮骨的效     

不同敷料组合对烧伤患者供皮区创面微环境的影响

Objective To investigate the effect of dressing materials in various combinations on bum wound microenvironment and healing condition. Methods Two hundred donor sites with wounds of 0.3 mm in depth in 186 burn patients, who needed skingrafting and admitted to our ward were enrolled in study, and they were divided into A ( with dressing composed of aiginate + cotton pad for donor area), B ( with dressing composed of vaseline gauze + cotton pad for donor area) , C (with dressing composed of algi-nate+ foam dressing for donor area), D ( with dressing composed of vaseline gauze + foam dressing for donor area) groups according to random table method. Effect of dressings on wound evaporation and pH value were observed. Bacterial colonization, degree of pain complained by patients after dressing change, and wound healing time in each group were compared. Results One hundred and eighty-four patients complied with the study, while 2 patients were excluded due to untimely falling-off of the dressing. Wound evaporaton in respectively, among them B group showed optimal effect of keeping humidity (P<0.01). Wound pH value in A, B, C, D groups was 7.22±0.06, 7.41±0.03, 7.05±0.03, 7.34±0.06, respectively, among them it was highest in B group. The positive rate of bacteria in D group was highest (22.4%), and lowest in C group (4.0%). Pain was lightest in C group (score was 0.98±0.12), and most serious in B group ( score was 8.14±0.82). The shortest wound healing time was seen in C group (6.7±0.8 d) , and longest in D group (15.6±3.5 d). Conclusions Application of various dressings on similar wounds can pro-duce different wound microenvironment, which is closely related to wound healing time. Compared with pH value, humidity is the more important factor for wound healing.     

不同敷料组合对烧伤患者供皮区创面微环境的影响

Objective To investigate the effect of dressing materials in various combinations on bum wound microenvironment and healing condition. Methods Two hundred donor sites with wounds of 0.3 mm in depth in 186 burn patients, who needed skingrafting and admitted to our ward were enrolled in study, and they were divided into A ( with dressing composed of aiginate + cotton pad for donor area), B ( with dressing composed of vaseline gauze + cotton pad for donor area) , C (with dressing composed of algi-nate+ foam dressing for donor area), D ( with dressing composed of vaseline gauze + foam dressing for donor area) groups according to random table method. Effect of dressings on wound evaporation and pH value were observed. Bacterial colonization, degree of pain complained by patients after dressing change, and wound healing time in each group were compared. Results One hundred and eighty-four patients complied with the study, while 2 patients were excluded due to untimely falling-off of the dressing. Wound evaporaton in respectively, among them B group showed optimal effect of keeping humidity (P<0.01). Wound pH value in A, B, C, D groups was 7.22±0.06, 7.41±0.03, 7.05±0.03, 7.34±0.06, respectively, among them it was highest in B group. The positive rate of bacteria in D group was highest (22.4%), and lowest in C group (4.0%). Pain was lightest in C group (score was 0.98±0.12), and most serious in B group ( score was 8.14±0.82). The shortest wound healing time was seen in C group (6.7±0.8 d) , and longest in D group (15.6±3.5 d). Conclusions Application of various dressings on similar wounds can pro-duce different wound microenvironment, which is closely related to wound healing time. Compared with pH value, humidity is the more important factor for wound healing.     

特重度烧伤甘油异体皮的应用与供皮区的选择

治疗特重度烧伤时 ,对深度烧伤创面进行早期切、削痂以大张异体皮覆盖并取自体皮混合移植至关重要。供皮区往往以头皮为主 ,而应用甘油异体皮覆盖创面 ,并用阴囊皮肤作二次供皮鲜有报道。我们在１３例特重度烧伤患者深度创面切痂后 ,应用甘油异体皮覆盖并选择Ⅱ度创面愈合后的足底、头皮 (多次供皮 )及阴囊 (两次供皮 )作为供皮区 ,进行混合移植 ,取得了满意的效果 ,现报道如下。１．１一般资料本组１３例 ,其中男１１例 ,女２例 ,平均年龄 (４０．４±１０．８)岁 ;烧伤面积最大占总体表面积 (ＴＢＳＡ)的９７% ,最小占ＴＢＳＡ的５２ % ,…