Expression of type Ⅰ interferon in monocyte-derived dendritic cells after Toll like receptor 3 triggered in patients with chronic hepatitis B

Objective To detect the expression of type Ⅰ interferon in monocyte-derived dendritic cells(MoDCs)after Toll like receptor(TLR)3 triggered in patients with chronic hepatitis B(CHB),and to evaluate immune responses of CHB patients and its roles in the mechanisms of persistent infection of hepatitis B virus(HBV)and chronicity of hepatitis.Methods Peripheral blood mononuclear cells(PBMCs)were isolated and purified using magnetic beads(plasma was saved simultaneously)from 26 CHB patients and 18 healthy volunteers(HV).Dendritic cells(DCs)were induced and proliferated in a culture medium with recombinant human granulocyte macrophage colony stimulating factor(rhGM-CSF)and recombinant human interleukin(rhIL-4).EX3s were stimulated with Poly Ⅰ:C and the supernatants were collected at 0 h and 24 h after stimulation.Type Ⅰ interferon(IFN-α and IFN-β)in plasma and supernatants were examined by enzyme linked immunosorbent assay (ELISA).Results The levels of type Ⅰ interferon in plasma were not significantly different in groups of HV and CH B.IFN-α and IFN-β expressions in supernatants before Poly Ⅰ:C stimulation were(80.00±16.15)ng/L,(36.39±13.90)ng/L in CHB group and(76.76±15.90)ng/L,(37.14±13.68)ng/L in HV group,respectively.And there were no statistical differences between two groups(t=1.651,t=0.178;both P＞0.05).IFN-α expressions in supernatants at 24 h after stimulation in two groups were both higher than those before stimulation(at 0 h),but there were no statistical differences(t=1.534,t=1.243;both P＞0.05).IFN-β expressions in supernatants at 24 h after stimulation in HV group was(54.57±16.80)ng/L,which was significantly higher than that at 0 h(37.14±13.68)ng/L(t=4.061,P＜0.05).However,there was no significant difference at 24 h than tht at 0 h in CHB group(t=1.796,P＞0.05).At 24 h after stimulation.IFN-β level was(54.57±16.80)ng/L in HV group,which was significantly higher than that[(41.64±12.57)ng/L]in CHB group(t=2.921,P＜0.05).Conclusions Functions of MoDCs from CHB patients are impaired and MoDCs could not express type Ⅰ interferon normally.Expression of type Ⅰ interferon after TLR3 triggered in CHB patients is mainly IFN-β.     

抗肿瘤雷公藤内酯醇半合成衍生物理化性质及其有关物质的研究

目的研究雷公藤内酯醇半合成衍生物(MC002)的理化性质和有关物质,为剂型设计提供依据。方法采用紫外分光光度法测定MC002在甲醇中的吸收系数;采用红外光谱仪测定MC002的红外吸收光谱;按中国药典2010版二部中溶解度的测定方法测定在不同溶剂中的近似溶解度;采用摇瓶法测定MC002在正辛醇-水、正辛醇-pH=7.4磷酸盐缓冲液中的油水分配系数;用Thiele管测定熔点;采用X-射线粉末衍射法测定其晶型。结果 MC002在甲醇中的吸收系数(E1%)为226.23±29.18;样品红外吸收光谱图与标准品谱图基本一致,易溶于水、甲醇、乙醇、丙酮,微溶于氯仿和乙酸乙酯,在乙醚中极微溶解;在正辛醇-水和正辛醇-pH=7.4磷酸盐缓冲液中的分配系数分别为0.074 0和1.080;熔点为50⁵2℃;X-射线粉末衍射说明MC002为非结晶型粉末。3批有关物质测定均<1%,样品纯度>99%。结论 MC002是一个亲水性很强的盐类药物,本实验确定的MC002部分理化性质和有关物质分析,可为其生物药剂学的研究及剂型设计提供依据。     

Expression of type Ⅰ interferon in monocyte-derived dendritic cells after Toll like receptor 3 triggered in patients with chronic hepatitis B

Objective To detect the expression of type Ⅰ interferon in monocyte-derived dendritic cells(MoDCs)after Toll like receptor(TLR)3 triggered in patients with chronic hepatitis B(CHB),and to evaluate immune responses of CHB patients and its roles in the mechanisms of persistent infection of hepatitis B virus(HBV)and chronicity of hepatitis.Methods Peripheral blood mononuclear cells(PBMCs)were isolated and purified using magnetic beads(plasma was saved simultaneously)from 26 CHB patients and 18 healthy volunteers(HV).Dendritic cells(DCs)were induced and proliferated in a culture medium with recombinant human granulocyte macrophage colony stimulating factor(rhGM-CSF)and recombinant human interleukin(rhIL-4).EX3s were stimulated with Poly Ⅰ:C and the supernatants were collected at 0 h and 24 h after stimulation.Type Ⅰ interferon(IFN-α and IFN-β)in plasma and supernatants were examined by enzyme linked immunosorbent assay (ELISA).Results The levels of type Ⅰ interferon in plasma were not significantly different in groups of HV and CH B.IFN-α and IFN-β expressions in supernatants before Poly Ⅰ:C stimulation were(80.00±16.15)ng/L,(36.39±13.90)ng/L in CHB group and(76.76±15.90)ng/L,(37.14±13.68)ng/L in HV group,respectively.And there were no statistical differences between two groups(t=1.651,t=0.178;both P＞0.05).IFN-α expressions in supernatants at 24 h after stimulation in two groups were both higher than those before stimulation(at 0 h),but there were no statistical differences(t=1.534,t=1.243;both P＞0.05).IFN-β expressions in supernatants at 24 h after stimulation in HV group was(54.57±16.80)ng/L,which was significantly higher than that at 0 h(37.14±13.68)ng/L(t=4.061,P＜0.05).However,there was no significant difference at 24 h than tht at 0 h in CHB group(t=1.796,P＞0.05).At 24 h after stimulation.IFN-β level was(54.57±16.80)ng/L in HV group,which was significantly higher than that[(41.64±12.57)ng/L]in CHB group(t=2.921,P＜0.05).Conclusions Functions of MoDCs from CHB patients are impaired and MoDCs could not express type Ⅰ interferon normally.Expression of type Ⅰ interferon after TLR3 triggered in CHB patients is mainly IFN-β.     

颌面部皮瘘一例误诊

[病例] 女,27岁.因反复发作左侧颌面部瘘管18个月就诊.18个月前无任何诱因出现左侧颌面部肿痛,在他院诊断为颌面部脓肿,予切开引流,红肿消退后形成瘘管,流脓1月余,瘘管封闭,之后分别于11个月、4个月前颌面部又出现肿痛,并二次行瘘管手术切除,近日复发来我院就诊.     

复方控释药条治疗急性冠周炎的疗效观察

急性冠周炎进行局部的冠周冲洗和置药是行之有效的治疗手段,我们制备了局部应用于冠周炎的复方控释药条,应用于临床取得了满意的疗效。1资料与方法1.1一般资料患者152例,均为门诊患者,其中男85例,女     

围术期护理干预对骨科手术患者心理状况的影响

目的 探讨围术期护理干预对骨科手术患者心理状况的作用.方法 选取2009-10-2010-10于冀中能源峰峰集团总医院进行骨科手术治疗的600例患者为研究对象,将其随机分为干预组(护理干预)300例和对照组300例,分别用汉密顿焦虑量表(HAMA)评定神经症状及患者焦虑症状的严重程度和艾森克个性问卷,以龚氏法作为评定进行统计及比较.结果 干预组在焦虑发生率和易激动、情绪波动及易怒出现频率明显低于对照组,差异有统计学意义(P＜0.05或P＜0.01).结论 围术期护理干预对骨科手术患者心里状况明显改善,能更好的保证手术的成功和患者的康复,值得推广应用.     

补肾方剂对糖尿病大鼠牙周炎的疗效观察

目的观察补肾方剂对糖尿病大鼠牙周炎的治疗效果。方法选择60只大鼠建立糖尿病牙周炎模型,按照给药的不同分为单纯牙周炎组(不给药)、灌喂中药组(补肾方剂)、灌喂氨基胍(AG)组;同时选择16只正常大鼠作为对照组,给药4周、6周后均制作组织切片,观察牙周组织形态结构的变化,测定牙龈中一氧化氮(NO)含量。结果灌喂中药组的结缔组织附着丧失量、牙槽骨高度丧失量、牙龈组织中NO的含量均明显低于其他糖尿病牙周炎组,差异有统计学意义(P<0.05)。结论补肾方剂对促进糖尿病牙周炎牙周组织的修复,降低NO含量的作用优于AG。     

胃体癌贲门癌术后肠内营养的护理

胃体癌、贲门癌根治的最常用的手术方式是全胃切除或胃大部分切除。胃切除术对正常的生理状态有一定的破坏,患者术后会出现营养代谢障碍。肠内营养支持不仅可给机体提供营养素,纠正机体紊乱状态,而且还可以增加应激状态下胃肠道的血流量,给肠黏膜提供营养素,保护肠黏膜,减少应激反应的程度。     

308 nm准分子激光治疗白癜风临床分析

目的 分析308 nm准分子激光治疗白癜风的临床疗效的相关因素。方法 用308 nm准分子激光进行局部治疗,每4天接受治疗1次,10次为一疗程。结果 治疗总体有效率为90.16%,显效率78.69%。按年龄分儿童有效率及显效率均高于老年人(60岁以上)。病程越短有效率及显效率越高。按病期分稳定期与进展期有效率及显效率无统计学差异。按部位区分,有效率及显效率为,头面部＞颈部＞躯干部＞四肢＞手足＞黏膜。不良反应发生率为47.54%。结论 308 nm准分子激光治疗白癜风安全有效,患者的年龄、病程、部位对疗效有影响,病期对疗效无显著影响。